• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.1241-1245
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• 2015

Background: Thyroid carcinoma is the most common malignancy of the endocrine organs. Although the majority of thyroid cancer patients experience positive outcomes, anaplastic thyroid carcinoma is considered one of the most aggressive malignancies. Current therapeutic regimens do not confer a significant survival benefit, and new therapies are urgently needed. Oncolytic herpes simplex virus (oHSV) may represent a promising therapy for cancer. In the present study, we investigated the therapeutic effects of a third-generation HSV vector, $G47{\Delta}$, on various human thyroid carcinoma cell lines in vitro. Two subcutaneous (s.c.) models of anaplastic thyroid carcinoma were also established to evaluate the in vivo anti-tumor efficacy of $G47{\Delta}$. Materials and Methods: The human thyroid carcinoma cell line ARO, FRO, WRO, and KAT-5, were infected with $G47{\Delta}$ at different multiplicities of infection (MOIs) in vitro. The survival rates of infected cells were calculated each day. Two s.c. tumor models were established using ARO and FRO cells in Balb/c nude mice, which were intratumorally (i.t.) treated with either $G47{\Delta}$ or mock. Tumor volumes and mouse survival times were documented. Results: $G47{\Delta}$ was highly cytotoxic to different types of thyroid carcinomas. For ARO, FRO, and KAT-5, greater than 30% and 80% of cells were killed at MOI=0.01 and MOI=0.1, respectively on day 5. WRO cells displayed modest sensitivity to $G47{\Delta}$, with only 21% and 38% of cells killed. In the s.c. tumor model, both of the anaplastic thyroid carcinoma cell lines (ARO and FRO) were highly sensitive to $G47{\Delta}$; $G47{\Delta}$ significantly inhibited tumor growth and prolonged the survival of mice bearing s.c. ARO and FRO tumors. Conclusions: The oHSV $G47{\Delta}$ can effectively kill different types of human thyroid carcinomas in vitro. $G47{\Delta}$ significantly inhibited growth of anaplastic thyroid carcinoma in vivo and prolonged animal survival. Therefore, $G47{\Delta}$ may hold great promise for thyroid cancer patients.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.39 no.5
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• pp.544-555
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• 2002

The aim of this study is to develop the $^1H$-MRS data postprocessing software for both single-voxel and multi-voxel technique, which plays and important role as a diagnostic tool in clinical field. This software is based on graphical user interface(GUI) under windows operating system of personal computer(PC). In case of single-voxel MRS, both of raw data in time-domain and spectrum data in frequency-domain are simultaneously displayed in a screen. Several functions such as DC correction, zero filling, line broadening, Lorentz-Gauss filtering and phase correction, etc. are included to increase the quality of spectrum data. In case of multi-voxel analysis, spectroscopic image reconstructed by 3-D FFT was displayed as a spectral grid and overlapped over previously obtained T1- or T2-weighted image for the spectra to be spatially registered with the image. The analysis of MRS peaks were performed by obtaining the ratio of peak area. In single-voxel method, statistically processed peak-area ratios of MRS data obtained from normal human brain are presented. Using multi-voxel method, MR spectroscopic image and metabolite image acquired from brain tumor are demonstrated.

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.262-269
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• 2016

Subway station scales are determined by peak predictions. In this study, the purpose behind the installation of a subway is public transportation convenience and public interest, but economic validity is also important. By proving that the scale of the station is excessive with regard to the target station size for Seoul subway Line 5-8, a reasonable plan. can be sought. According to station installation standards, the area of the station under investigation here is out of the service levels by six stages (A~F), and it must be four or more levels (D). The Actual level for the B level is a two-step design. The Actual ratio for over- Peak predictions is only 17.8% on average. The results of measurements of the excess area and determination of the excessive costs were analyzed by subdividing the area and by calculating it based on the B level, finding that it is possible to provide benefits for customers only in the current design, with an area ratio of 16.3%. Given the weight, it was estimated that current conditions can meet the needs of only 18.6% of the current area. Simplifying the scale calculation method of the station, it is convenient, safe, and advantageous to move citizens only if the scale can be streamlined. Then, with a reduced initial investment, maintenance costs during the operation can be reduced.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.3
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• pp.12-21
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• 2012

The proximities of Cheonggye creek was a maj or space for the gathering of large populations from the past times and it is also a place containing the joy, anger, sorrow, pleasure and lives of the citizens. The Cheonggye creek which flowed downtown of Seoul throughout many historical events had disappeared into history completely in the modernization process due to the contamination with life sewages and overflow. However, the city authority of Seoul decided to restore it to its shape before covering the creek by means of dismantling the old elevated ways in Cheonggye creek in line with the Large Scale City Movement Project to revive the grey city and to improve the quality of the city landscape in 2002. The cultural space was created by activating the old town sphere in Cheonggye Creek and the amenities of the giant city was improved by the cultural and natural wave created along the creek. In addition, the educational opportunity has been provided to the citizens by means of reviving its historical nature. The design and cultural value of the Cheonggye Plaza were restored for the mean time and the urban environment requested during modem times was established. The Cheonggye Plaza which heightened the balanced development in Seoul which has been developed in an unbalanced way by dividing into northern and southern area of the river is very meaningful in the viewpoint of the landscape architect who designs the urban space. The re-birth as a cultural space of downtown owing to the efforts of the said cultural catalysts is meaningful.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.6
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• pp.393-398
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• 2012

Mast cells are involved in allergic responses, protection against pathogens and autoimmune diseases. Dexamethasone (Dex) and other glucocorticoids suppress $Fc{\varepsilon}RI$-mediated release of inflammatory mediators from mast cells. The inhibition mechanisms were mainly investigated on the downstream signaling of Fc receptor activations. Here, we addressed the effects of Dex on Fc receptor expressions in rat mast cell line RBL-2H3. We measured mRNA levels of Fc receptors by real-time PCR. As expected, Dex decreased the mRNA levels of activating Fc receptor for IgE ($Fc{\varepsilon}R$) I and increased the mRNA levels of the inhibitory Fc receptor for IgG $Fc{\gamma}RIIb$. Interestingly, Dex stimulated transcriptions of other activating receptors such as Fc receptors for IgG ($Fc{\gamma}R$) I and $Fc{\gamma}RIII$. To investigate the mechanisms underlying transcriptional regulation, we employed a transcription inhibitor actinomycin D and a translation inhibitor cycloheximide. The inhibition of protein synthesis without Dex treatment enhanced $Fc{\gamma}RI$ and $Fc{\gamma}RIII$ mRNA levels potently, while $Fc{\varepsilon}RI$ and $Fc{\gamma}RIIb$ were minimally affected. Next, we examined expressions of the Fc receptors on cell surfaces by the flow cytometric method. Only $Fc{\gamma}RIIb$ protein expression was significantly enhanced by Dex treatment, while $Fc{\gamma}RI$, $Fc{\gamma}RIII$ and $Fc{\varepsilon}RI$ expression levels were marginally changed. Our data showed, for the first time, that Dex regulates Fc receptor expressions resulting in augmentation of the inhibitory receptor $Fc{\gamma}RIIb$.

• The Journal of Korean Institute of Information Technology
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• v.15 no.12
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• pp.181-188
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• 2017

As a population of leisure activities grows and diversifies, there is a great demand for portable and environment-friendly power generation systems. A small wind power generation system is emerging as a suitable power generation equipment to meet these needs. The most important thing when developing a small portable wind turbine is to reduce the weight of the generator and increase the efficiency. The existing 300W wind turbine generator weighs about 10kg, which is heavy to carry. Therefore, a new generator weighing less than 4kg to make it easy to carry with high efficiency has been developed. In addition, considering complicated characteristics of wind volume and topography of Korea, a small wind turbine that can be used in urban and rural areas individually was constructed. Through basic designing and optimization, the lightweight and efficient generator was manufactured. It is a 300W wind turbine designed and fabricated with reduced weight as a prototype. The average output voltage of the generator was 24.7V at 900rpm no-load test. On a load test with the average line voltage 36.8V and the average phase current 2.62A, when the mechanical input was 339.84W, an average voltage output of the generator was measured as 289.5W with efficiency of 85.18%. The generator weight was 3.84kg.

• Nutrition Research and Practice
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• v.13 no.1
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• pp.58-63
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• 2019

BACKGROUND/OBJECTIVES: Telomeres are located at the chromosomal ends and progressively shortened during each cell cycle. Telomerase, which is regulated by hTERT and c-MYC, maintains telomeric DNA sequences. Especially, telomerase is active in cancer and stem cells to maintain telomere length for replicative immortality. Recently we reported that walnut phenolic extract (WPE) can reduce cell viability in a colon cancer stem cell (CSC) model. We, therefore, investigated the effect of WPE on telomere maintenance in the same model. MATERIALS AND METHODS: $CD133^+CD44^+$ cells from HCT116, a human colon cancer cell line, were sorted by Fluorescence-activated cell sorting (FACS) and treated with WPE at the concentrations of 0, 10, 20, and $40{\mu}g/mL$ for 6 days. Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA ($ddC_t$). Telomerase activity was also measured by qRT-PCR after incubating the PCR mixture with cell protein extracts, which was adjusted with reference DNA ($dC_t$). Transcriptions of hTERT and c-MYC were determined using conventional RT-PCR. RESULTS: Telomere length of WPE-treated cells was significantly decreased in a dose-dependent manner ($5.16{\pm}0.13$ at $0{\mu}g/mL$, $4.79{\pm}0.12$ at $10{\mu}g/mL$, $3.24{\pm}0.08$ at $20{\mu}g/mL$ and $3.99{\pm}0.09$ at $40{\mu}g/mL$; P = 0.0276). Telomerase activities concurrently decreased with telomere length ($1.47{\pm}0.04$, $1.09{\pm}0.01$, $0.76{\pm}0.08$, and $0.88{\pm}0.06$; P = 0.0067). There was a positive correlation between telomere length and telomerase activity (r = 0.9090; P < 0.0001). Transcriptions of both hTERT and c-MYC were also significantly decreased in the same manner. CONCLUSION: In the present cell culture model, WPE reduced telomere maintenance, which may provide a mechanistic link to the effect of walnuts on the viability of colon CSCs.

• Journal of the Korean earth science society
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• v.40 no.2
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• pp.177-187
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• 2019

Several volcanic activities have continued in Mt. Baekdu since the Millennium eruption, and these phenomena have increased the need for volcanic activity surveillance. Various geophysical approaches are needed to obtain the depth and size of magma chamber that lie several kilometers below the surface. We examined the applicability of direct-current resistivity survey in this study. In order to explore the deep magma chamber of Mt. Baekdu, which has a spatial limitation due to the borderline, a large-scale survey with a length of tens of kilometers should be conducted. This type of survey requires a distributed measurement system and optimized exploration designs. Therefore, we propose survey designs taking advantage of our developed distributed acquisition system and analyze the applicability using numerical simulation. We confirmed that our designs that use single survey line with offline transmitting points show comparable results to the conventional 3D survey. It is expected that our research result can contribute to the deep geophysical exploration in Mt. Baekdu.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.87-97
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• 2021

To investigate the current pest management status in Korea, a survey was conducted from 151 students and graduates in the Korea National College of Agriculture and Fisheries (KNCAF) by on-line. The questionnaire consists of two divisions, basic questions and pest control questions. The basic questions were including the respondent's age, academic status, cultivating crops and cultivating area. The pest control questions were including pest control methods, pesticide selection rationale, and pest forecasting methods. As a summary of basic questions, the respondents in their 20s accounted for 91.2%. Moreover, 34.5% of the respondents had over 3 hectares of cultivating area. The cultivating methods were differed by cultivating crops. As a summary of pest control questions, major control methods were using the conventional chemicals (>66%). To understand the pesticide selection rationale, farmers/respondents made their own decisions based on existing control techniques (30%) or depended on the decisions of pesticide vendors (29%). As for the pest forecasting method, it was mainly conducted by the Rural Development Administration affiliated organization (29%) and the National Crop Pest Management System (27%). Regarding the reliability of the pest diagnosis and pesticide prescription of pesticide vendors, 97% of the respondents marked above average. However, there was no choice on strong reliability. Interestingly, 79% of the respondents agreed to train experts for pest diagnosis and pesticide prescription with high necessity and, in particular, 47% of respondents were very strongly supported. These results suggest that the farmers might be need more qualified experts in pest diagnosis and pesticide prescriptions. Taken together, these survey results would provide important information to understand the current status of pest management by farmers' point of view and useful to set the direction of pest control.

• Korean Journal of Poultry Science
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• v.48 no.4
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• pp.161-168
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• 2021

The present study investigated production traits, including body weight, egg production, egg weight, and egg quality in Korean indigenous parent stocks. Parent stocks produced from a nine-combination association from five pure line strains (C, D, F, K, and Y) were used. The body weight analyses results showed that the CY combination had the highest body weight (1,604.9 g) and the DK combination had the lowest (1,424.4 g). The average age at first egg of the nine combinations was 118.6 days; however, that of the YD combination was 111.6 days, making it the fastest growing combination, whereas the DK combination was the slowest, at 126 days. Hen-day egg production was 74% or more for the CF, CK, and DK combinations. The DK combination hens showed excellent persistence in egg laying. Hen-housed egg production exhibited results similar to those of hen-day egg production. Egg weight was significantly higher in the DK combination than in the other combinations and was observed to increase from 20 (43.9 g) to 40 (58.1 g) weeks. The egg quality analyses results showed that the combinations based on the F and K strains had a bright eggshell color, with relatively high egg weights. Eggshell strength and thickness were the highest in the DK combination (3.8 kg/cm², 0.38 mm). In summary, the CF and DK combinations showed excellent egg production ability and egg quality, while the YC, YD, and YK combinations with the Y strain as the paternal strain exhibited poor performance.