• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.897-905
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• 2003

Phospholipase D (PLD) and ADP-ribosylation factor (ARF) were partially purified on a series of column chromatography, and their biochemical properties were characterized to understand the regulatory mechanism of PLD activation by ARF protein in the antigen-induced immune responses in guinea pigs. Heparin Sepharose and high-Q Sepharose column chromatographies were used for the purification of PLD, and Sephadex G-25, DEAE Sephacel, Source 15 PHE (HIC), Superdex-75, and Uno-Q column chromatographies were used for the purification of ARF. The purified PLD and ARF proteins were identified with anti-rabbit PLD- or ARF-specific antibodies, showing about 64 or 85 kDa for the molecular mass of PLD and 29 or 35 kDa for the sizes of ARF. Partial cDNA of ARF3 was cloned by RT-PCR in guinea pig lung tissue and its nucleotides and amino acids were sequenced. Guinea pig ARF3 showed 92% of nucleotides sequence identity and 100% of amino acid sequence homology with human ARF3. The ARF-regulated PLD activity was measured in the oleate or ARFs-containing mixed lipid vesicles. The purified and recombinant ARF (rARF) activities were assessed with the $GTP{\gamma}S$ binding assay. The PLD activity was induced by oleate in a dose-dependent manner. The purified ARF and recombinant ARF3 increased PLD activity in guinea pig lung tissues. These data show that the activity of membrane-bound PLD can be regulated by the cytosolic ARF proteins, suggesting that ARF proteins in guinea pig lung can act as a regulatory factor in controlling the PLD activity in allergic reaction.

• International Journal of Internet, Broadcasting and Communication
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• v.10 no.2
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• pp.13-18
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• 2018

Drones are controlled by the remote pilot from the ground stations using the radio control or autonomously following the pre-programmed flight plans. In this paper, we develop a method and an optimal path search system for providing 3D augmented reality flight (ARF) images for safe and efficient flight control of drones. The developed system consisted of the drone, the ground station and user terminals, and the optimal path search server. We use the Dijkstra algorithm to find the optimal path considering the drone information, flight information, environmental information, and flight mission. We generate a 3D augmented reality flight (ARF) image overlaid with the path information as well as the drone information and the flight information on the flight image received from the drone. The ARF image for adjusting the drone is generated by overlaying route information, drone information, flight information, and the like on the image captured by the drone.

• Water for future
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• v.25 no.3
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• pp.79-86
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• 1992

ARF(Areal Reduction Factor) have been developed and used to convert point I-D-F to areal I-D-F in many countries. In Korea, through ARF was calculated in Han river basin by several researchers, it has limit to apply to other regions \ulcorner 새 low density of rainfall gauge station and shortage of data. In this study ARF has developed in areas of high density of rainfall gauge station, Pyungchang river(han river), Wi stream(nakdong river), and Bochung stream(Guem river) basin by fixed-area method. And coefficient of variation of annual mean precipitation was presented to use ARF in othere areas and its applicability was analyzed.

• Clinical and Experimental Pediatrics
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• v.50 no.3
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• pp.277-283
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• 2007

Purpose : Acute renal failure (ARF) is an important complication of rhabdomyolysis. The purpose of this study was to identify the major causes of rhabdomyolysis in children and to identify the factors associated with the developmet of ARF. Methods : A retrospective chart review between January 1997 to June 2005 was conducted of 60 patients with a diagnosis of rhabdomyolysis. Rhabdomyolysis was defined by an elevation of serum creatine phosphokinase (CK) greater than 1,000 IU/L with a MM fraction more than 95% or serum myoglobin>300 mg/dL or positive urine myoglobin. Patients were excluded if they had evidence of myocardial ischemia, or cerebrovascular insufficiency. Results : Sixty patients (37 males, 23 females) were enrolled, with the median age of 4.25 year. The most common causes of rhabdomyolysis were repiratory tract infection (9), seizure (7), hypoxia or asphyxia (6). Fifteen patients (25.0%) developed ARF and ten of them (66.0%) died. The initial serum creatinine, uric acid, potassium, pH and peak serum creatinine, initial systolic blood pressure, and mental status were statistically correlated with the development of ARF. The peak serum CK was associated with mortality of rhabdomyolysis. Conclusion : Acute renal failure was significant complication of rhabdomyolysis in children. Several clinical and laboratory factors were statistically associated with the development of ARF and death.

• Journal of Chest Surgery
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• v.40 no.1 s.270
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• pp.32-36
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• 2007

Background: Although acute renal failure (ARF) after coronary artery bypass graft (CABG) is relatively rare, but devastating complication with high mortality. Our study aims to evaluate the effectiveness of early application of CRRT in patients with ARF which developed after on-pump CABG. Material and Method: Two hundred and eighty seven patients underwent isolated on-pump CABG between May 2002 and Feb. 2006 at our institution, of whom 15 (5.2%) needed CRRT (11 patients for postoperatively developed ARF and the remaining 4 patients with preexisting dialysis-dependent chronic renal failure (CRF) for postoperative hemodynamic and metabolic control). Criteria for early application of CRRT were as follows; decreased urine output less than 0.5cc/h/kg for 2 consecutive hours and elevated serum creatinine level greater than 2.0 mg/dL. Result: The incidence of ARF requiring CRRT after on-pump CABG was 3.9% (11/283) and the overall hospital mortality of patient with CRRT was 33.3% (5/15). Of 5 deaths, 4 were patients with postoperatively developed ARF, and 1 was a patient with pre-existing dialysis-dependent CRF patient. The mean time between the operation and the initiation of CRRT was $25.8{\pm}5.8$ hours and the mean duration of CRRT was $62.1{\pm}41.2$ hours. Of the 7 survivors who were not on dialysis-dependent preoperatively, 6 patients fully recovered renal function during hospital stay and 1 patient required permanent renal supportive treatment after discharge from hospital. Conclusion: Early application of CRRT could maintain stable postoperative hemodynamic status and make outcomes better than those of previous reports in patients with ARF which developed after on-pump CABG.

• Journal of Biomedical Engineering Research
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• v.12 no.2
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• pp.79-88
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• 1991

Semi-transparent volume rendering technique can provide 3-D visualization well by voxel level Processing and alleviate segmentation arf, ifacts compared wish the surface rendering technique. In this Paper, we consider several new schemes which can improve she Perform ance of volume rendering. A directional interpolation method is proposed to reduce the artifact due to the anisotrophic resolution in X-ray CT data. The computation time for rendering is shortened by using the depth information of the 3-D object. And also, we reduce the quantization artifacts in the rendering by introducing the opacity-dependent sampling interval to sampling in ray-tracing.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.43.2-43.2
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• 2018

송전 및 배전선 선로에 사용되는 핵심 부품인 ACSR (Aluminum Conductor Steel Reinforced, 강심 알루미늄 연선) cable은 우수한 기계적 성질, 가벼운 중량, 내부식성 특징을 가지고 있어 송전 및 배전선 선로에 핵심 부품으로 사용된다. 하지만, 국내외 혹한 다설 지역에 설치된 ACSR cable에서 빙설해로 인한 단락 또는 지락 사고가 지속적으로 발생하고 있다. 빙설해에 의한 송전선로의 고장은 급격한 전압 강하로 인해 전기 품질에 큰 영향을 주어 민원제기의 주요 원인이 되며, 고장의 파급효과가 국지적으로 발생하지 않고 광범위하게 발생하는 특징이 있기 때문에 이에 대한 대응이 필요한 실정이다. 이러한 문제를 해결하기 위해 ACSR cable의 주 소재인 알루미늄에 대한 판상(Plate) 결빙강도 파악 및 결빙방지 소재개발 연구가 국내외에서 활발히 진행 중이나, 실제 원형의 전선다발이 나선형으로 감겨있는 구조의 ACSR cable 결빙 접합강도를 시험을 통해서 명확히 제시한 연구결과는 아직 보고된 바 없다. 본 연구에서는 실제 송전용 ACSR cable을 대상으로 얼음 간의 주 전단 응력, 파단에너지 등의 결빙특성을 정량적으로 측정할 수 있는 3D 스캔을 활용한 결빙특성 평가시험기를 개발하고, 345kV급 ACSR cable에 대한 결빙특성을 평가결과를 제시하였다. 또한 ACSR cable에 현재 상용화되고 있는 결빙방지 코팅소재를 적용함으로써 코팅소재의 적합성을 ARF(Adhesion reduction factor) 지표를 통해서 비교 평가한 결과를 포함한다.

• Journal of Chest Surgery
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• v.31 no.5
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• pp.494-501
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• 1998

From May 1, 1993 to May 31 1995, the authers studied retrospectively 211 patients who underwent cardiovascular operation with cardiopulmonary bypass(CPB). Because we were interested in new development of ARF(prevalence, mortality rate, and main risk factors), we performed a multivariate statistical analysis about data of patients with preoperative serum creatinine values of less than 1.5 mg/dL. Normal renal function before operation(serum creatinine level less than 1.5 mg/dL) was registered in 198(74%) patients. Of these, 27(14%) patients showed postoperative renal complication, including 20(10%) patients classified as renal dysfunction(serum creatinine level between 1.5 and 2.5 mg/dL) and 7(4%) patients as acute renal failure(serum creatinine level higher than 2.5 mg/dL). The mortality rate was 5.8% in normal patients, 5% in patients with renal dysfunction, and 43% when acute renal failure developed(p=0.036). Indeed, the renal impairment proved to be an independent predictor of mortality(odd ratio 2.52∼11.25), along with cardiovascular(odd ratio 4.20) and respiratory(odd ratio 2.18) complications. Multivariate analysis identified the following variables as independent risk factors for postoperative renal impairment : advanced age(odd ratio 1), need for emergency operation(odd ratio 3.78), low-output syndrome(odd ratio 3.66), respiratory complication(odd ratio 1.30), need for deep hypothermic circulatory arrest(odd ratio 1.4). The 13 patients(7%) with preoperative renal failure showed a significantly higher morbidity and mortality rate than those without renal complications before operation. We concluded that the likelihood of severe renal complications is resonably low in the patients undergoing cardiac operation without preexisting renal dysfunction, but associated mortality remains high. A prominant role of hemodynamic factor in the development of postoperative acute renal failure must be recognized during preoperative, intraoperative, and postoperative periods.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.6
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• pp.2629-2633
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• 2012

Background: Raw betel nut (RBN) chewing is an important contributing factor for esophageal squamous cell carcinoma (ESCC), although associated genomic changes remain unclear. One difficulty in assessing the effects of exclusively RBN induced genetic alterations has been that earlier studies were performed with samples of patients commonly using tobacco and alcohol, in addition to betel-quid. Both CDKN2A (at 9p21) and Rb1 gene (at 13q14.2) are regarded as tumor suppressors involved in the development of ESCC. Therefore, the present study aimed to verify the RBN's ability to induce ESCC and assess the involvement of CDKN2A and Rb1 genes. Methods: A panel of dinucelotide polymorphic markers were chosen for loss of heterozygosity studies in 93 samples of which 34 were collected from patients with only RBN-chewing habit. Promoter hypermethylation was also investigated. Results: Loss in microsatellite markers D9S1748 and D9S1749, located close to exon $1{\beta}$ of CDKN2A/ARF gene at 9p21, was noted in 40% ESCC samples with the habit of RBN-chewing alone. Involvement of a novel site in the 9p23 region was also observed. Promoter hypermethylation of CDKN2A gene in the samples with the habit of only RBN-chewing alone was significantly higher (p=0.01) than Rb1 gene, also from the samples having the habit of use both RBN and tobacco (p=0.047). Conclusions: The data indicate that the disruption of 9p21 where CDKN2A gene resides, is the most frequent critical genetic event in RBN-associated carcinogenesis. The involvement of 9p23 as well as 13q14.2 could be required in later stages in RBN-mediated carcinogenesis.