• Journal of Conservation Science
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• v.33 no.1
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• pp.43-49
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• 2017

The ancient paper document we chose as a case study for our research is "One Hundred Poets of the Tang Dynasty", which is housed in the Ryukoku University Library. The purpose of this research is to introduce a method to analyze the surface roughness and microstructure at a high resolution. In addition, we attempt to quantitatively measure the surface unevenness of the types and curve structure. We used a tridimensional digital microscopy as a non-contact and a non-destructive method to study ancient cultural paper. The information contained in the paper may be lost in the process of applying strong pressure to clean and lining or press. However, this microscopic measurement method can non-destructively analyze a large amount of data in old printed books. Moreover, it enables observing them directly with reflected light. Therefore, this method may be useful for collecting printed information remaining on the surface of the paper.

• The Journal of the Acoustical Society of Korea
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• v.41 no.5
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• pp.507-517
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• 2022

The PhotoAcoustic Microscopy (PAM) has been proved to be a useful tool for biological and medical applications due to its high spatial and contrast resolution. PAM is based on transmission of laser pulses and reception of PA signals. Since the strength of PA signals is generally low, not only are high-performance optical and acoustic modules required, but high-performance electronics for imaging are also particularly needed for high-quality PAM imaging. Most PAM systems are implemented with a combination of several pieces of equipment commercially available to receive, amplify, enhance, and digitize PA signals. To this end, PAM systems are inevitably bulky and not optimal because general purpose equipment is used. This paper reports a PA signal receiving system recently developed to attain the capability of improved Signal to Noise Ratio (SNR) and Contrast to Noise Ratio (CNR) of PAM images; the main module of this system is a low noise, wideband signal receiver that consists of two low-noise amplifiers, two variable gain amplifiers, analog filters, an Analog to Digital Converter (ADC), and control logic. From phantom imaging experiments, it was found that the developed system can improve SNR by 6.7 dB and CNR by 3 dB, compared to a combination of several pieces of commercially available equipment.

• Journal of ILASS-Korea
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• v.21 no.2
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• pp.88-94
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• 2016

In this study digital holographic microscopy system for measurements of 3-D velocities of particles in MR fluid is developed. Holograms are recorded using either a CCD camera with a double pulse laser or a high-speed camera with a continuous laser. To process recorded holograms, the correlation coefficient method is used for focal plane determination of particles. To remove noise and improve the quality of holograms and reconstructed images, a Wiener filter is adopted. The two-threshold and image segmentation methods are used for binary image transformation. For particle pairing, the match probability method is adopted. The developed system will be applied to measurements of the characteristics of unsteady 3-D particle velocities in MR fluids through the next stage of this study.

• Proceedings of the Mineralogical Society of Korea Conference
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• 2003.05a
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• pp.80-80
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• 2003

포천-기산리 일대에 분포하는 쥬라기의 대보화강암류의 변형정도를 결정하기 위해 석영의 파동소광의 강도(IWE : Intensity of Wavy Extinction)를 편광현미경, 디지털 카메라, NIH Image로 측정하였다. 파동소광이 나타나는 석영입자의 한 부분이 편광현미경 상에서 최대 소광이 될 때 IWE를 측정하고, 이 때 나타나는 파동소광의 아입자경계에 수직한 방향으로 측정을 시도하였다 본 연구에 사용된 NIH Image는 파동소광을 보여주는 각 부분의 광도를 256단계의 흑백 농담변화로 나타낼 수 있다. 이를 이용하여 측정 길이에 대한 농담변화로 IWE를 구하였다. 이렇게 획득한 IWE를 5$^{\circ}$ 단위로 묶어 돗수분포표로 처리하였으며, 자료의 분포특성상 히스토그램의 최빈값과 중간값 사이의 중간값을 그 암석의 대표 IWE로 정의하였다. 이를 다시 5단계의 변형대(무변형, 저변형, 중변형, 고변형, 최고변형)와 각각의 변형지수(D1, D2, D3, D4, D5)로 묶었다. 본 연구지역에 분포하는 화강암류는 흑운모화강암(Gb), 동부의 석류석흑운모화강암(Ggb) 그리고 북부의 복운모화강암(Gtm)으로 구성되어 있는 바 본 연구에는 크게 포천읍을 기준으로 서부에 분포하는 Gb와 동부에 분포하는 Ggb에서 파동소광의 강도 측정이 이루어 졌다. 연구결과 Gb는 D2 이하의 낮은 변형도가 나타났고, Ggb는 D3이상의 고변형이 나타나, 전반적으로 조사지역의 북서부에서 남동부로 갈수록 변형도가 증가하는 양상을 보여주었다.

• Journal of radiological science and technology
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• v.40 no.1
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• pp.121-126
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• 2017

A technique for producing the ear shell for a hearing aid using DICOM (Digital Imaging and Communication in Medicine) image and a 3D printing was studied. It is a new application method, and is an application technique that can improve the safety and infection of hearing aid users and can reduce the production time and process stages. In this study, the effects on the shape surface were examined before and after the printing of the ear shell using a 3D printer based on the values obtained from the raw data of the DICOM images at the volumes of 0.5 mm, 1.0 mm, and 2.0 mm, respectively. Before the printing, relative relationship was compared with respect to the STL (STereoLithography) file structure; and after the printing, the intervals of the layered structure of the ear shell shape surface were compared by magnifying them using a microscope. For the STL file structure, the numbers of triangular vertices, more than five intersecting points, and maximum intersecting points were large in the order of 0.5 mm, 1.0 mm, and 2.0 mm, respectively; and the triangular structure was densely distributed in the order of the bending, angle, and crest regions depending on the sinuosity of the external auditory meatus shape. As for the ear shell shape surface examined by the digital microscope, the interval of the layered structure was thick in the order of 2.0 mm, 1.0 mm, and 0.5 mm. For the STL surface structure mentioned above, the intersecting STL triangular structure was denser as the sinuosity of the 3D ear shell shape became more irregular and the volume of the raw data decreased.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.07a
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• pp.331-332
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• 2011

디저털 홀로그래피는 CCD를 이용하여 간섭 패턴을 기록하고 기록된 간섭무늬에 담겨진 물체의 정보로 입체영상을 복원하는 기술을 말하며, 현미경을 이용한 디지털 홀로그래피 기술은 3D 형상 복원이나 바이오포토닉스 등 다양한 분야에서 사용되고 있다. 하지만, 디지털 홀로그래피 기술은 촬영된 간섭패턴에서 픽셀의 위상차가 레이저 파장의 1/2 이상일 경우, 기존의 phase unwrapping 알고리즘으로는 해당 픽셀의 위상차이를 계산할 수 없는 문제가 있다. 이와 같은 문제를 해결하기 위해 본 논문에서는 다수의 홀로그램 영상과 깊이맵을 사용하여 완전한 형태의 깊이맵을 구하는 방법을 제안한다.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.06c
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• pp.433-438
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• 1998

본 연구에서는 time averaging과 depth profiling기술을 음향현미경에 적용하여 5MHz 대역의 초음파 영상처리 시스템을 구성하였으며 기존의 피크값 검출기술과 상호 비교, 분석하였다. time averaging기술에서는 한 지점에서 반사된 tone burst파 전체를 디지털 오실로스코프를 통해 시간영역에서 A/D변환하고 변환된 512개 데이터들의 평균값을 취함으로써 영상을 얻을 수 있었으며, 이 기술은 시간영역에서 smoothing효과를 이용하여 산란이 심한 영역에 대한 영상을 개선시킬 수 있었다. depth profiling기술은 기준신호에 대한 반사신호의 시간 지연값을 최소 분해능 2ns로 검출함으로써 샘플의 3차원적인 실제 기하학적인 모양을 상대적인 크기로 얻을 수 있었다.

• Journal of Korea Multimedia Society
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• v.10 no.7
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• pp.846-855
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• 2007

Significant feature extraction in cancer cell image analysis is an important process for grading cell carcinoma. In this study, we propose a method for 3D quantitative analysis of cell nuclei based upon digital image cytometry. First, we acquired volumetric renal cell carcinoma data for each grade using confocal laser scanning microscopy and segmented cell nuclei employing color features based upon a supervised teaming scheme. For 3D visualization, we used a contour-based method for surface rendering and a 3D texture mapping method for volume rendering. We then defined and extracted the 3D morphological features of cell nuclei. To evaluate what quantitative features of 3D analysis could contribute to diagnostic information, we analyzed the statistical significance of the extracted 3D features in each grade using an analysis of variance (ANOVA). Finally, we compared the 2D with the 3D features of cell nuclei and analyzed the correlations between them. We found statistically significant correlations between nuclear grade and 3D morphological features. The proposed method has potential for use as fundamental research in developing a new nuclear grading system for accurate diagnosis and prediction of prognosis.

• The Journal of Engineering Geology
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• v.12 no.4
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• pp.405-419
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• 2002

Fracture roughness of rock specimens is observed by a new confocal laser scanning microscope (CLSM; Olympus OLS1100). The wave length of laser is 488 nm, and the laser scanning is managed by a light polarization method using two galvano-meter scanner mirrors. The function of laser reflection auto-focusing enables us to measure line data fast and precisely. The system improves resolution in the light axis (namely z) direction because of the confocal optics. Using the CLSM, it is Possible to measure a specimen of the size up to $10{\;}{\times}{\;}10{\;}cm$ which is fixed on a specially designed stage. A sampling is managed in a spacing $2.5{\;}\mu\textrm{m}$ along x and y directions. The highest measurement resolution of z direction is $10{\;}\mu\textrm{m}$, which is more accurate than other methods. Core specimens of coarse and fine grained granite are provided. Fractures are artificially maneuvered by a Brazilian test method. Measurements are performed along three scan lines on each fracture surface. The measured data are represented as 2-D and 3-D digital images showing detailed features of roughness. Line profiles of the coarse granites represent more frequent change of undulation than those of the fine granite. Spectral analyses by the fast Fourier transform (FFT) are performed to characterize the roughness data quantitatively and to identify influential frequency of roughness. The FFT results suggest that a specimen loaded by large and low frequency energy tends to have high values of undulation change and large wave length of fracture roughness.

• Journal of the Institute of Convergence Signal Processing
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• v.12 no.3
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• pp.181-187
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• 2011

In this thesis, digital in-line holographic microscopy has been implemented with enhanced phase shifting technique. It was demonstrated that the zero-order diffraction noise and the twin image can be eliminated by phase-shifting interferometry very effectively. Also the experimental and numerical reconstruction has been incorporated into one set-up operating in real time. Experimental results and the analysis of the phase map indicate that the proposed system can be very useful for the measurement of microscopic objects and 3-D microscopy.