Digital Holographic microscopy based on phase shifting technique

위상천이가법에 의한 디지털 홀로그래피 마이크로스코피에 관한 연구

  • Received : 2011.05.20
  • Accepted : 2011.08.02
  • Published : 2011.07.30

Abstract

In this thesis, digital in-line holographic microscopy has been implemented with enhanced phase shifting technique. It was demonstrated that the zero-order diffraction noise and the twin image can be eliminated by phase-shifting interferometry very effectively. Also the experimental and numerical reconstruction has been incorporated into one set-up operating in real time. Experimental results and the analysis of the phase map indicate that the proposed system can be very useful for the measurement of microscopic objects and 3-D microscopy.

본 논문에서는 홀로그램정보의 손실이 없고, CCD 화소면의 최대 활용이 가능한 in-line 형태의 광학계를 구성하고, 보다 향상된 위상 천이법을 토대로 디지털 in-line 홀로그래피 마이크로스코피를 구현하였다 이러한 구성방식으로 디지털 홀로그래피의 최대 문제인 0차 회절 항에 의한 DC잡음과 in-line 홀로그래피에서 나타나는 트윈 이미지가 효율적으로 제거됨을 실험적으로 보였고, 위상 맵핑을 통해 계량적으로 분석하였다. 또한 광학장치 컨트롤과 영상처리가 연동하여 실시간적으로 이루어지므로, 본 논문에서 연구한 DHM 방식은 앞으로 미세분석을 위한 삼차원 현미경법에 많은 응용이 이루어 질것으로 기대된다.

Keywords

References

  1. Ting-Chung Poon, "Optical Scanning Holography with MATLAB", Springer pp.21-40, pp.49-64, Jan, 2007.
  2. Yves Emery, Etienne Cuche, Tristan Colomb, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Pierre Magistretti, "DHM(Digital Holography Microscope) for imaging cells", Journal of Physics : Conference Series 61, pp.1317-1321, 2007.
  3. Etienne Cuche, Pierre Marquet, Christian Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical roconstruction of Fresnel off-axis holograms", Applied Optics, Vol. 38, No. 34, pp.6994-7001 Dec, 1999. https://doi.org/10.1364/AO.38.006994
  4. W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, "Digital in-line holography of microspheres", Appl. Opt. Vol. 41, No. 25, Sep, 2002
  5. Myung K Kim, Lingfeng Yu, Christopher J Mann, "Interference techniques in digital holography", J. Opt. A : Pure Appl. pp.S518-S523, Opt, 2006.
  6. John F. Restrepo, Jorge A. Herrera R., Roman Castaneda, "Comparison of reconstruction methodologies for off-axis and in-line digital holographic microscopy", 2008 OSA, BIOMED/DHlLACSEA, 2008.
  7. Toyohiko Yatagai, Mark L. Tachiki, "Simultaneous depth determination of multiple objects by focus analysis in digital holography", 2008 OSA / BIOMED/DHlLACSEA, 2008.
  8. Emique Tajalmerce, Osamu Matoba, Steven C, Ven-all, Ballram J avidi, "Optoelectronic information encryption with phase-shifting interferometry" Appl. Opt. vol. 39, no. 14, pp.2313-2320, 2000 https://doi.org/10.1364/AO.39.002313