• Journal of Microbiology and Biotechnology
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• 제18권9호
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• pp.1606-1612
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• 2008

Interleukin (IL)-32 is a recently identified proinflammatory cytokine that is one of the IL-18 inducible genes, and plays an important role in autoimmune and inflammatory diseases. We produced antibodies against IL-32 and studied the expression of IL-32 in human stomach cancer. We detected IL-32 secreted from K-562 cells which were stably transfected with IL-32 and in the sera of stomach cancer patients by a sandwich ELISA using a monoclonal antibody KU32-52 and a polyclonal antibody. In order to optimize a sandwich immunoassay, recombinant IL-32a was added, followed by the addition of a biotinylated KU32-52 into microtiter plate wells precoated with a goat anti-IL-32 antibody. The bound biotinylated KU32-52 was probed with a streptavidin conjugated to HRP. This sandwich ELISA was highly specific and had a minimal detection limit of 80 pg/ml (mean${\pm}$SD of zero calibrator) and measuring up to 3,000 pg/ml. This ELISA showed no cross-reaction with other cytokines such as hIL-1$\alpha$, hIL-1$\beta$, hIL-2, hIL-6, hIL-8, hIL-10, hIL-18, and hTNF-$\alpha$. Intra-assay coefficients of variation were 18.5% to 4.6% (n=10), and inter-assay coefficients were 23% to 9% (n=10). The average IL-32 level in the sera of 16 stomach cancer patients (189 pg/ml) was higher than that of 12 healthy control men (109 pg/ml). Our results indicate that serum IL-32 level can be detected by using an established ELISA, and that this immunoassay and mAb KU32-09 specific for immunohistochemistry can be used in the detection of expressed and secreted IL-32 in stomach cancer patients.

• 호남수학학술지
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• 제32권2호
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• pp.333-362
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• 2010

We define H*H-fuzzy set and form a new category Set(H*H) consisting of H*H-fuzzy sets and morphisms between them. First, we study it in the sense of topological universe and obtain an exponential objects of Set(H*H). Second, we investigate some relationships among the categories Set(H*H), Set(H) and ISet(H).

• Journal of Plant Biology
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• 제10권1_2호
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• pp.36-40
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• 1967

1. The effect of the pH value on P32 absorption of leaves of three forest trees under the water culture is investigated. 2. The degree of the P32 absorption to the pH value is quite different in each forest tree. 3. The phosphorus contents are proved higher in the leaves of Quercus accutissima(the saw shaped oak) than in the leaves of Pinus densiflora(the red pine) and Lespedeza bicolar (bush clover). 4. Larger contents of phosphorus are found in acidity plots than in alkalinity. The leaves of treated plot of the pH 4 (red pine), of the pH 5.0 (bush clover), and of the pH 5.0-6.5 (saw shaped oak) have a higher nutrient capital as indicated by P32 compared with that of the other pH values. 5. It is also noticed that P32 absorption capacity is decreased with the higher pH values. 6. The content of P of leaves shows the lowest value in the plot of the pH 6.5 (pine), of the pH 7.5(bush clover), and of the pH 9.0(saw shaped oak). It is also noticed that the red pine is to do very well in acid cultivation and then follow bush clover and saw shaped oak in the order.

• 생명과학회지
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• 제27권8호
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• pp.930-936
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• 2017

Pseudomonas sp. GP32가 생산하는 생물고분자응집제 Biopol32의 실제 산업폐수에서의 적용을 위하여 생물고분자 Biopol32의 응집특성을 조사하였다. Biopol32의 응집물질은 polysaccharide로 확인되었다. 음이온성 응집제들은 응집효율을 높이기 위하여 보조응집제로 counter ion을 사용하고 있다. Biopol32의 응집활성은 보조응집제로 $Ca^{2+}$, $Al^{3+}$와 같은 양이온을 첨가 하였을 때 크게 증가하였으며, 보조응집제로서 7.0 mM $CaCl_2{\cdot}2H_2O$을 첨가하였을 때 Biopol32의 응집활성이 가장 높게 나타났다. Kaolin 현탁액에 Biopol32를 1.5 mg/l의 농도로 첨가하였을 때 가장 높은 응집활성을 보였다. Biopol32의 pH와 온도에 따른 응집활성은 현재 폐수처리 현장에서 상업적으로 이용되고 있는 음이온성 유기합성고분자응집제 polyacrylamide와 Zoogloea ramigera로부터 생산된 생물고분자응집제 zooglan과 응집활성을 비교하였다. Biopol32의 응집활성은 pH 5.0에서 8.0의 넓은 범위의 pH에서 높은 응집활성을 보였으며, 또한 온도의 영향에서는 $60^{\circ}C$에서 가장 높은 응집활성을 나타내었으나, $70^{\circ}C$ 이상의 온도에서는 응집활성이 급격히 감소하는 것으로 나타났다. Jar fermentor를 이용한 batch culture를 통하여 Biopol32의 응집활성과 전하밀도와의 관계를 조사하였다. 음이온성 전하밀도와 겉보기 점도가 높을수록 Biopol32의 응집활성이 높아져, Biopol32의 응집활성과 겉보기점도는 Biopol32의 전하밀도와 밀접한 관계가 있는 것으로 나타났다.

• Bulletin of the Korean Chemical Society
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• 제32권10호
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• pp.3559-3560
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• 2011

• 호남수학학술지
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• 제32권4호
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• pp.763-767
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• 2010

In this note, we give a solution of a $H^{\infty}$-optimization problem.

• Bulletin of the Korean Chemical Society
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• 제32권spc8호
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• pp.3145-3148
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• 2011

• Bulletin of the Korean Chemical Society
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• 제32권11호
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• pp.4001-4004
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• 2011

Various 5-substituted 1H-tetrazole derivatives were synthesized in a simple and environmentally benign method from the reaction of aryl and benzyl nitriles with sodium azide in solvent-free media using montmorillonite K-10 clay as solid recyclable heterogeneous acidic catalyst and microwave irradiation in good yields and short reaction times.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1174-1179
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• 2009

An experiment was conducted to study the effect of post-hatch feed deprivation on yolk sac utilization andsubsequent performance of young broiler chickens (280) up to 35 days of age. The experimental treatments included access to feed at 8 h intervals after hatch, up to 48 h (0, 8, 16, 24, 32, 40 or 48 h). Water was offered ad libitum to all the groups immediately after placement. Results indicated that chicks with access to feed immediately after hatch used up the residual yolk more quickly. Access to feed between 8-24 h post-hatch, supported faster utilization of residual yolk compared to those chicks that remained unfed for 40-48 h (p<0.05). Further, deprivation of feed up to 24 h did not alter the lipid and protein contents in residual yolk, but fasting of chicks beyond 24 h (32, 40 and 48 h) led to retention of higher lipid and lower protein content in the yolk sac (p<0.05). At 7 days of age, the weights of proventiculus and gizzard were not affected by feed deprivation up to 48 h. However, the liver, pancreas and jejunum recorded significantly (p<0.05) heavier weights in chicks that were fed during the initial 24 h period compared to delayed feeding (32-48 h). Chicks fed within 24 h after hatch gained significantly (p<0.05) higher weight at 5 weeks of age than those that received feed between 32 and 48 h. Feed deprivation for 48 h was more detrimental to growth than 24-40 h. This study revealed the significance of early posthatch feeding (<24 h) on faster utilization of yolk sac nutrients and optimum development of intestines and organs, culminating in improved weight gain (>10.5%) of broilers at 5 weeks of age.

• Korean Journal of Mathematics
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• 제32권1호
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• pp.97-107
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• 2024

Let A ⊆ B be an extension of integral domains with characteristic zero. Let H(A, B) and h(A, B) be rings of composite Hurwitz series and composite Hurwitz polynomials, respectively. We simply call H(A, B) and h(A, B) composite Hurwitz rings of A and B. In this paper, we study when H(A, B) and h(A, B) are unique factorization domains (resp., GCD-domains, finite factorization domains, bounded factorization domains).