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Studies on the Evaluation of the Spent Composts of Selenium-Enriched Mushrooms as a Feed Selenium Source (셀레늄강화 버섯폐배지에 대한 사료 셀레늄공급원으로의 평가 연구)

  • Kim, W.Y.;Min, J.K.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.7 no.1
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    • pp.118-130
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    • 2005
  • This study was conducted to evaluate the spent composts of selenium-enriched mushrooms as a feed selenium Source. Total selenium (Se) contents and Se profiles in the spent mushroom composts (SMC) were determined. In addtion, we also investigated the metabolism in relation to Se accumulation in the mushroom. Mushrooms used in this study were Flammulina velutipes and Se enriched mushrooms were grown for 60 days by adding 2 mg of inorganic Se (Na2SeO3) per kg of mushroom composts (MC) on as-fed basis and it was compared with mushrooms not to add Se to the MC. Total Se contents for Se-treated mushrooms were significantly increased (P<0.0001) by 20-fold (4.51 ㎍/g of dry) compared to Se-untreated (0.23 ㎍/g of dry). On the contrary, organic Se proportion was significantly lower (P<0.0001) in the Se-treated mushroom (72.3%) than Se-untreated (100%, not analytically detected of inorganic Se). Se distribution upon a length in the Se-treated mushrooms was the highest in the bottom part (6.86 ㎍/g of dry) near to MC, and top and middle parts were significantly lower (3.71 and 3.01 ㎍/g of dry, respectively) than the bottom (P<0.001). In the SMC from Se-treated mushrooms, a high concentration of Se (5.04 ㎍/g of dry) was still remained, but that from Se-untreated mushrooms was significantly low (P<0.0001) as 0.08 ㎍/g of dry. Se-treated SMC showed a high rate of organic Se (65.67%), suggesting that most of inorganic Se in the SMC was converted to organic Se by mushroom mycelia, and Se-untreated SMC showed 100% of organic Se, not being detected of inorganic Se. Prior to mycelia inoculation in the mushroom culture, the sterilization of MC brought approximately 18% of Se loss in the MC. This result is in accordance with facts generally known that Se is weak in the high temperature and it is consequently volatilized under that condition. Apparent and net accumulation rates (%) for Se into mushrooms were 14.81 and 10.14%, respectively and their difference (4.67%) is considered that it is due to the volatilization into the air via metabolic process of mushroom itself. From the result of this study, inorganic Se addition to MC for mushroom improved the Se content in the mushroom and SMC from Se-enriched mushrooms contained a high concentration of Se. Mycelium and fruiting body from mushrooms converted inorganic Se in MC to organic Se, indicating a high proportion of organic Se in the mushroom and SMC. Therefore, Se in Se-enriched mushroom and SMC was recognized as Se sources of food for human as well as feed for livestock.

A Model Experiment Study to Secure the Straight Line Distance between the Air Inlet and Exhaust Section of the Living Room (거실제연설비중 공기유입구와 배출구간 직선거리 확보를 위한 모형실험연구)

  • Saeng-Gon Lee;Se-Hong Min
    • Journal of the Society of Disaster Information
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    • v.19 no.2
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    • pp.439-450
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    • 2023
  • Purpose: When conducting fire inspections in Korea, there are objects that violate the fire protection regulations that require a straight line distance of more than 5m between the air inlet and the discharge section if the floor area is less than 400m2, and this paper analyzes the reasons and conducts a model experimental study to support the need for related fire protection regulations. Method: Domestic firefighting objects were investigated and confirmed, domestic and foreign papers, policies, and laws and regulations were examined, and spaces with a straight line distance of less than 5m and more than 5m between the air inlet and discharge section were selected and analyzed through model experiments in a living room of less than 400m2 . Result: When examining the domestic fire protection regulations (NFPCNational Fire Perpormance Code), the separation distance between the air inlet and the outlet is more than 5m when the floor area is less than 400m2 , but as a result of the actual investigation, it was confirmed that there are firefighting objects that cannot keep the separation distance. In addition, when a paper review of overseas fire protection regulations for a straight line distance of more than 5m showed that there was no regulation on the straight line distance between the air inlet and the discharge section, the model experiment showed that the discharge speed was better when the straight line distance between the air inlet and the discharge section was more than 5m than when it was less than 5m. Conclusions: In this study, when examining overseas fire laws and regulations by comparing the performance of the fire protection ratio for the straight line distance between the air inlet and the exhaust section, there is no mandatory regulation for the straight line distance, but the domestic fire protection regulations (NFPCNational Fire Perpormance Code) require more than 5m. It is hoped that this will be reflected in the design stage in the future, and a foundation will be laid to reduce the responsibility and burden of fire superintendents.

Investigation of Heavy Metal Migration from Food Contact Materials used for Food Delivery Using an Inductively Coupled Plasma-Mass Spectrometer

  • Chae-Yeon Hwang;Young-Jun Kim
    • Journal of Food Hygiene and Safety
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    • v.38 no.2
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    • pp.37-45
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    • 2023
  • The surge in food delivery systems during the coronavirus 2019 pandemic necessitated this study of heavy metal migration from food contact materials (FCMs). A total of 104 samples of FCMs, comprising 51 polypropylene (PP), 21 polyethylene (PE), and 32 polystyrene (PS) samples of six different types of FCMs (containers, covers, table utensils, cups, pouches, and wrappers) used for food delivery distributed in Korea, were collected and investigated for migration of three heavy metals (Pb, Cd, and As) using inductively coupled plasma-mass spectrometry (ICP-MS) to determine whether they complied with Korea's Standards and Specifications for Utensils, Containers, and Packages. Acetic acid (4%, v/v) was used as the food simulant, and tests were performed at 100℃ (in harsh conditions) for 30 min. Linearity of Pb, Cd, and As showed acceptable results with a coefficient of determination (R2) value of 0.9999. Limit of detection (LOD) and limit of quantification (LOQ) of Pb, Cd, and As were 0.001, 0.001, and 0.001 ㎍/L and 0.002, 0.003, and 0.003 ㎍/L, respectively. Accuracy and precision results complied with the criteria presented in the European Commission Joint Research Centre guidelines. The average concentration of Pb, Cd, and As migration detected in a total of 104 samples was 0.009-0.260 ㎍/L, which was very low compared with the migration specification set in the Standards and Specifications for Utensils, Containers, and Packages. The maximum level of Pb corresponded to 0.23% of the migration limit. There were no samples exceeding the limit. Thus, this study confirmed that the heavy metal contents of FCMs used for delivery food distributed in Korea were safely managed. The data from this study represent an invaluable source for science-based safety management of hazardous heavy metals migrating from FCMs used in the food delivery industry.

Clinical Observation on East-West Integration Treatment in Stroke and Brain Disease (중풍 뇌 질환의 한 방향 협진에 관한 임상적 고찰)

  • Shin, Ae-sook;Lee, ln-whan;Kim, Na-hee;Kim, Hye-rni;Kim, Min-kyung;Sim, So-ra;Cho, Seung-yeon;Park, Seong-uk;Park, Jung-mi;Bae, Hyung-sup;Ko, Chang-nam
    • The Journal of the Society of Stroke on Korean Medicine
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    • v.11 no.1
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    • pp.9-17
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    • 2010
  • Objectives : This study was aimed to evaluate the current status of East-West integrated treatment in stroke and brain disease and to discuss further plans. Methods : The medical records of patients who visited the stroke and brain disease center at Kyung Hee University, East-West Neo Medical Center from May 2006 to August 2010 were evaluated. The general characteristics of patients who underwent integrated treatment, trend in the number of cross referrals were initially evaluated. Later major disorders, the reasons of referrals and the number of visits in outpatients were analyzed. Results : 1. 3496 patients were referred from the eastern medical hospital to the western medical hospital and 2440 patients from the western medical hospital to the eastern medical hospital. The number of patients reached a peak alter the opening of the hospital and has decreased from then on. Referrals of female patients were more than those of male patients and patients over 50 years old were the most. 2. Admitted patients with stroke of chronic stage were most commonly referred from the eastern medical hospital to the western medical hospital and cerebral infarction was most common from the western medical hospital to the eastern medical hospital. Among the outpatients cerebral infarction topped from east to west, and stroke of chronic stage from west to east. 3. 36.6% of the patients from east to west received integrated treatment more than 3 times and 28.6% from west to east. Headache was the second most common reason to be referred from west to east and 36.7% of patients didn't continue to have either of the treatment and 30.3% received eastern treatment only, Conclusions: According to this study, chronic stroke management was successfully performed in the outpatient clinic in the form of East-West integration treatment. Further research on other diseases such as headache is recommended.

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Evaluation of Cultivation Characteristics according to NO3- Ratio of Nutrient Solution for Korean Melon in Hydroponic Culture (양액의 NO3- 비율이 수경재배 참외의 생육과 수량에 미치는 영향)

  • Do Yeon Won;Ji Hye Choi;Chang Hyeon Baek;Na Yun Park;Min Gu Kang;Young Jin Seo
    • Journal of Bio-Environment Control
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    • v.32 no.3
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    • pp.249-255
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    • 2023
  • Korean melon (Cucumis melo L.) is grown mostly in Northeast Asia area, and as a fruit mainly produced in Korea, the yield per unit area continues to improve, but the cultivation method is limited to soil cultivation, so it is necessary to develop hydroponic cultivation technology for scale and labor-saving is needed. As the ratio of NO3- increased, the plant height, the leaf length, the leaf width, and the internode length became longer and larger. On the other hand, the SPAD value decreased. The lower the ratio of NO3-, the faster the female flower bloom, and there was no difference in fruit maturity between treatments. There was no difference in the shape of fruit according to the ratio of NO3-, and the hardness was higher as the ratio of NO3- was lower. The total yield from March to July was KM3 5,650 kg/10a and KM1 4,439 kg/10a, 27% higher in KM3 and, in particular, 36% higher in quantity from March to May, when Korean melon prices were high season. Therefore, it was judged that it would be appropriate to supply NO3- suitable for hydroponic cultivation of Korean melon, which was formalized in December and produced from spring, at the level of 6.5 to 10 me·L-1.

Effect of Band Application of Slow Release Fertilizer on Rice Growth and Yield in Puddled-soil Drill Seeding (벼 무논골뿌림재배시 완효성비료의 측조 시비가 생육 및 수량에 미치는 영향)

  • Kim, Sang-Su;Choi, Min-Gyu;Park, Keon-Ho;Lee, Seon-Yong;Cho, Su-Yeon;Cho, Dong-Sam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.1
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    • pp.68-76
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    • 1996
  • To elucidate the optimum fertilizer level and application method for band application under puddled-soil drill seeding in Jeonbuk series of fluvio-marine alluvial soil at National Honam Agricultural Experiment Station in 1995, using Dongjinbyeo, slow releasing compound fertilizer of 100% and 80% to conventional application level was applied totally as basal fertilizer simultaneously with seeding under 3cm and 5cm depth from soil surface in a distance of 4cm from the seeded row. Plant height was taller and tiller number was higher in band application than conventional application but ratio of effective tiller was vice versa. Panicle number was more but ratio of effective tiller ratio was lower in 100% than 80% level of band application and they were higher in 3cm than 5cm depth from soil surface. Leaf area index and dry weight was higher in conventional application at early growth stage but was vice versa after maximum tillering stage, and they were higher in 3cm depth at early growth stage but 5cm depth after maximum tillering stage. NH$_4$-N in soil was higher in conventional application at 25 days after seeding but, thereafter was lower than band application and it was higher in 3cm than 5cm depth till 40 days after seeding but was versa, thereafter. Lodging degree was slightly higher in band application, 100% level and 5cm depth than in their counterparts. Panicle number and grain number per $m^2$ was lower in conventional application than 80% or 100% level of band application without significant difference between band application levels or application methods. Yield was higher at 80% level of band application under 3cm depth than conventional application, but no significantly different among other application methods. Therefore, 80% level of band application under 3cm depth of soil surface was more effective for puddled-soil drill seeding on the basis of the reduction of application efforts, better plant growth and higher yield in rice.

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Does Sarcopenic Obesity Affect Physical Function and Physical Fitness of Korean Older Women? (근위축비만이 국내 여성고령자의 신체기능과 체력에 미치는 영향)

  • Hong, Seung-youn
    • 한국노년학
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    • v.30 no.3
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    • pp.831-842
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    • 2010
  • BACKGROUND: Sarcopenic obesity(SO), a condition of the reduction in muscle mass paired with an increased fat mass has been paid attention because of its association with disability in later life. A few evidence, however, has reported the association with these factors. PURPOSE: To explore the association among SO, physical function and fitness in older women. METHOD: 257 older women(age of 74) were recruited from Y city and 7 physical functions and 4 fitness tests were measured. Participants were classified into one of four groups based on their body fat and muscle mass: Normal group (GR-A), high fat(GR-B), sarcopenia(GR-C), and sarcopenic obese(GR-D). GLMand LSD-test were conducted with SPSS 12.0. RESULTS: Chair stand, arm-curl, back-scratch, 2-min steps of GR-A was higher than GR-C and GR-D(p<.05). One-leg stand of GR-A was higher than GR-D(p<.01) and of GR-C was higher than GR-D(p<.01).8ft-TUG of GR-D was lower than GR-A(p<.01). Grip strength, knee extension of GR-A was higher than that of GR-C and GR-D(p<.01) and knee flexion of GR-A was also higher than that of GR-C and GR-D(p<.01). CONCLUSION: Based on our findings, we conclude that SO is significantly associated with lower physical function and fitness in older Korean women, which alarm the risk of frailty induced by SO.

Distribution and Frequency of SSR Motifs in the Chrysanthemum SSR-enriched Library through 454 Pyrosequencing Technology (국화 SSR-enriched library에서 SSR 반복염기의 분포 및 빈도)

  • Moe, Kyaw Thu;Ra, Sang-Bog;Lee, Gi-An;Lee, Myung-Chul;Park, Ha-Seung;Kim, Dong-Chan;Lee, Cheol-Hwi;Choi, Hyun-Gu;Jeon, Nak-Beom;Choi, Byung-Jun;Jung, Ji-Youn;Lee, Kyu-Min;Park, Yong-Jin
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.546-551
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    • 2011
  • Chrysanthemums, often called mums or chrysanths, belong to the genus Chrysanthemum, which includes about 30 species of perennial flowering plants in the family Asteraceae. We extracted DNA from Dendranthema grandiflorum ('Smileball') to construct a simple sequence repeat (SSR)-enriched library, using a modified biotin-streptavidin capture method. GS FLX (Genome Sequencer FLX System which provides the flexibility to perform the broad range of applications) sequencing (at the 1/8 run specification) resulted in 18.83 mega base pairs (Mbp) with an average read length of 280.06 bp. Sequence analyses of all SSR-containing clones revealed a predominance of di-nucleotide motifs (16,375, 61.5%) followed by tri-nucleotide motifs (6,616, 24.8%), tetra-nucleotide motifs (1,674, 6.3%), penta-nucleotide motifs (1,283, 4.8%), and hexa-nucleotide motifs (693, 2.6%). Among the di-nucleotide motifs, the AC/CA class was the most frequently identified (93.5% of all di-nucleotide types), followed by the GA/AG class (6.1%), the AT/TA class (0.4%), and the CG/GC class (0.03%). When we analyzed the distribution of different repeat motifs and their respective numbers of repeats, regardless of the motif class, of 100 SSR markers, we found a higher number of di-nucleotide motifs with 70 to 80 repeats; we also found two di-nucleotide motifs with 83 and 89 repeats, respectively, but their product lengths were within optimum size (297 and 300 bp). In future work, we will screen for polymorphisms of possible primer pairs. The results will provide a useful tool for assessing molecular diversity and investigating the population structure among and within Chrysanthemum species.

Functional Components and Antioxidant Activities of Perilla Leaf Genetic Resource (잎들깨 유전자원의 기능성분 및 항산화 활성)

  • Yeon Ju An;Jung In Kim;Sang Woo Kim;Sungup Kim;Eunyoung Oh;Jeongeun Lee;Eunsoo Lee;Eunae Yoo;Jung-Sook Sung;Myoung Hee Lee;Choon-Song Kim;Min Young Kim
    • The Korean Journal of Food And Nutrition
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    • v.36 no.5
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    • pp.379-386
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    • 2023
  • The objective of this study was to investigate the distribution of functional compounds in perilla leaves of various genetic resources and their antioxidant activities. A comprehensive analysis of functional compounds was conducted for 90 genetic resources, focusing on total polyphenol content (TPC), total flavonoid content (TFC), individual phenolic content (IPC), and lutein. Their antioxidant activities were then analyzed based on their radical scavenging capacity using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The TPC content exhibited a range of 13.19 to 35.85 mg gallic acid equivalent/g, whereas the TFC content varied from 11.74 to 46.51 mg catechin equivalent/g. Total IPC was detected in a range of 6,310.98 to 40,491.82 ㎍/g. Lutein was detected at levels between 70.97 and 597.97 ㎍/g. ABTS and DPPH radical scavenging activities of perilla leaves ranged from 30.39 to 58.58 mg trolox equivalent (TE)/g and from 7.74 to 46.56 mg TE/g, respectively. Furthermore, correlation analysis demonstrated that rosmarinic acid, a phenolic acid, exhibited a significantly positive correlation with antioxidant activity. These findings suggest that various genetic resource of perilla leaves could effectively mediate antioxidant capacity. Results of this study provide valuable information for use of perilla leaves in Korea as functional food materials.

Effect of Accelerated Storage on the Microstructure and Water Absorption Characteristics of Korean Adzuki Bean (Vigna angularis L.) Cultivar (팥의 가속화 저장에 따른 미세구조 및 수분흡수 특성)

  • Jieun Kwak;Seon-Min Oh;You-Geun Oh;Yu-Chan Choi;Hyun-Jin Park;Suk-Bo Song;Jeong-Heui Lee;Jeom-Sig Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.167-174
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    • 2023
  • This study investigated the microstructure and water absorption characteristics of the Korean adzuki bean (Vigna angularis L.) cultivar under accelerated storage. The germination rate, acid value, redness (a*), and yellowness (b*) values showed no significant differences after three months of storage compared to pre-storage under low temperatures (4℃). However, a statistically significant difference was observed under accelerated high temperatures (45℃). In particular, after storage for three months, the germination rate and acid value were 0% and 33.63 mg KOH/100g, respectively, under accelerated high temperatures. After storage for three months, the holes, hilum damage, and spaces between the seed coat and cotyledon shortened the time and speed of water absorption under accelerated high temperatures compared to that under low temperatures. Conversely, further research is required to investigate the reason for the low rate of parallel water absorption.