• Biomolecules & Therapeutics
• /
• 제14권3호
• /
• pp.137-142
• /
• 2006

Apigenin, a natural flavonoid found in a variety of vegetables and fruits, has been shown to possess many biological functions. In this study we investigated the role of apigenin in the production of reactive oxygen species (ROS) through the modulation of activity of $K^+-Cl^-$-cotransport (KCC) in IMR-32 human neuroblastoma cells. Apigenin induced $Cl^-$-dependent $K^+$ efflux, a hallmark of KCC activity, which was markedly prevented by different kinds of KCC inhibitors (calyculin-A, genistein and $BaCl_2$). These results indicate that KCC is functionally present, and activated by apigenin in the IMR-32 cells. Treatment with apigenin also induced a sustained increase in the level of intracellular ROS. The KCC inhibitors also significantly inhibited the apigenin-induced ROS generation. Taken together, these results suggest that apigenin can modulate ROS generation through the activation of a membrane ion transporter, KCC. These results further suggest that the alteration of KCC activity may play a role in the mechanism of degenerative diseases and/or carcinogenesis in neuronal tissues through the regulation of ROS production.

• 한국생물물리학회:학술대회논문집
• /
• 한국생물물리학회 2003년도 정기총회 및 학술발표회
• /
• pp.40-40
• /
• 2003

Spontaneous firing rate and patterns of dopaminergic neurons in midbrain are key factors in determining the level of dopamine at target loci as well as in the mechanisms such as reward and motor coordination. Although glutamate, as a major afferent, is reported to enhance firing rate, the detailed actions of NMDA-, AMPA/kainate-, and metabotropic glutamate receptors (mGluR) on filing patterns are not clear. Thus we have investigated the role of glutamate receptors on the spontaneous firing activities using the network-free, acutely isolated dopamine neurons from substantia nigra pars compacta(SNc) of the 9-14 days rat. The isolated cells showed spontaneous regular firings of near 2.5 Hz, whose rate was enhanced by glutamate at submicromolar levels (0.3 $\square$M) but abolished by high concentrations more than 10 $\square$M.

• 전자공학회논문지C
• /
• 제34C권2호
• /
• pp.1-11
• /
• 1997

We developed a base station modulator ASIC for CDMA digital cellular system. In CDMA digital cellular system, the modulation is performed by convolutional encoding and QPSK with spread spectrum. The function blocks of base station modulator are CRC, convolutional encoder, interleaver pseudo-moise scrambler, power control bit puncturing, walsh cover, QPSK, gain controller, combiner and multiplexer. Each function block was designed by the logic synthesis of VHDL codes. The VHDL code was described at register transfer level and the size of code is about 8,000 lines. The circuit simulation and logic simulation were performed by COMPASS tools. The chip (ES-C2212B CMB) contains 25,205 gates and 3 Kbit SRAM, and its chip size is 5.25 mm * 5,45 mm in 0.8 mm CMOS cell-based design technology. It is packaged in 68 pin PLCC and the power dissipation at 10MHz is 300 mW at 5V. The ASIC has been fully tested and successfully working on the CDMA base station system.

• Journal of Communications and Networks
• /
• 제18권3호
• /
• pp.420-428
• /
• 2016

In this paper, we investigate the scheduling and power allocation for coordinated multi-point transmission in downlink long term evolution advanced (LTE-A) systems, where orthogonal frequency division multiple-access is used. The proposed scheme jointly optimizes user selection, power allocation, and modulation and coding scheme (MCS) selection to maximize the weighted sum throughput with fairness consideration. Considering practical constraints in LTE-A systems, the MCSs for the resource blocks assigned to the same user need to be the same. Since the optimization problem is a combinatorial and non-convex one with high complexity, a low-complexity algorithm is proposed by separating the user selection and power allocation into two subproblems. To further simplify the optimization problem for power allocation, the instantaneous signal-to-interference-plus-noise ratio (SINR) and the average SINR are adopted to allocate power in a single cell and multiple coordinated cells, respectively. Simulation results show that the proposed scheme can improve the average system throughput and the cell-edge user throughput significantly compared with the existing schemes with limited feedback.

• Molecules and Cells
• /
• 제43권2호
• /
• pp.198-202
• /
• 2020

Comprehensive inhibition of RUNX1, RUNX2, and RUNX3 led to marked cell suppression compared with inhibition of RUNX1 alone, clarifying that the RUNX family members are important for proliferation and maintenance of diverse cancers, and "cluster regulation of RUNX (CROX)" is a very effective strategy to suppress cancer cells. Recent studies reported by us and other groups suggested that wild-type RUNX1 is needed for survival and proliferation of certain types of leukemia, lung cancer, gastric cancer, etc. and for their one of metastatic target sites such as born marrow endothelial niche, suggesting that RUNX1 often functions oncogenic manners in cancer cells. In this review, we describe the significance and paradoxical requirement of RUNX1 tumor suppressor in leukemia and even solid cancers based on recent our findings such as "genetic compensation of RUNX family transcription factors (the compensation mechanism for the total level of RUNX family protein expression)", "RUNX1 inhibition-induced inhibitory effects on leukemia cells and on solid cancers through p53 activation", and "autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells". Taken together, these findings identify a crucial role for the RUNX cluster in the maintenance and progression of cancers and suggest that modulation of the RUNX cluster using the pyrrole-imidazole polyamide gene-switch technology is a potential novel therapeutic approach to control cancers.

• The Korean Journal of Physiology and Pharmacology
• /
• 제21권2호
• /
• pp.205-213
• /
• 2017

Quercetin, a plant-derived flavonoid found in fruits, vegetables and tea, has been known to possess bioactive properties such as anti-oxidant, anti-inflammatory and anti-cancer. In this study, anti-cancer effect of quercetin and its underlying mechanisms in triple-negative breast cancer cells was investigated. MTT assay showed that quercetin reduced breast cancer cell viability in a time and dose dependent manner. For this, quercetin not only increased cell apoptosis but also inhibited cell cycle progression. Moreover, quercetin increased FasL mRNA expression and p51, p21 and GADD45 signaling activities. We also observed that quercetin induced protein level, transcriptional activity and nuclear translocation of Foxo3a. Knockdown of Foxo3a caused significant reduction in the effect of quercetin on cell apoptosis and cell cycle arrest. In addition, treatment of JNK inhibitor (SP 600125) abolished quercetin-stimulated Foxo3a activity, suggesting JNK as a possible upstream signaling in regulation of Foxo3a activity. Knockdown of Foxo3a and inhibition of JNK activity reduced the signaling activities of p53, p21 and GADD45, triggered by quercetin. Taken together, our study suggests that quercetin induces apoptosis and cell cycle arrest via modification of Foxo3a signaling in triple-negative breast cancer cells.

• 한국전자파학회논문지
• /
• 제13권3호
• /
• pp.263-268
• /
• 2002

OFDM(orthogonal frequency division multiplexing) 시스템은 ISI와 주파수 선택적 채널에서 매우 강건한 (robust) 특성을 갖기 때문에 차세대 고속 데이터 전송에 효과적인 기술이다. 그러나, OFDM 시스템에서는 많은 부반송파를 사용하므로 높은 PAPR(peak to averahe power ratio)이 발생된다. 높은 PAPR의 OFDM 신호가 송신단의 비선형 증폭기를 통과할 때 심각한 왜곡이 발생한다. 본 논문에서는 프리엠파스시스 및 클리핑 기법을 이용하여 PAPR를 감소하였다. 이 방법은 IFFT 출력 신호를 프리엠파시스(de-emphasis)시키는 과정을 이용하여 효과적인 성능 개선을 갖는다. 부반송파의 수가 16개, QPSK 변조방식을 사용하고, 프리엠파시스 변화점이 IFFT 출력신호 최대진폭의 3/9이고, IFFT 출력진폭이 11인 지점에서 클립을 할때, CCDF(complementary cumulative density function)확률이 10/녀ㅔ -3/에서 PAPR이 약5.7㏈ 정도이고, BER=$10^{-3}$에서 요구 SNR은 기존의 OFDM보다 약 2 ㏈성능 개선을 보인다.

• Parasites, Hosts and Diseases
• /
• 제31권3호
• /
• pp.223-230
• /
• 1993

Cryptosporidium의 sporozoite에 대한 단클론항체(C6B6 C4Al)와 merozoite에 대한 단클론항체(Cmg-3)를 크립토스포리디움 오오시스트로 감염시킨, 생후 괄일된 생쥐에 4일 혹은 8일간 경구 투여하여 크립토스포리디움 감염에 미치는 효과를 조사하였다. 투여는 Cmg-3만으로, 혹은 C6B6, C4A1, Cmg-3를 함께 섞은 것으로, 그리고 식염수로 실행하였다. p < 0.05 수준에서 항체투여를 받은 것과 받지 않은 그룹 사이에 현격한 차이가 관찰되었다. Crng-3 한개만으로 투여하였을 때는 4일간 투여하였을 때만 효과가 있었던 반면, 3개의 단클론항체를 함께 투여하였을 때는 4일간. 8일간 모두 기생충의 증식을 현격히 감소시켰다. 이것은 경구투여된 항체가 장내에 기생하는 크립토스포리디움의 증식을 억제한 것을 보여주는 것이며 크립토스포리디움증에 대한 치료약이 없는 상황에서 치료법 개발에 새로운 접근 방법을 제시한다고 판단된다.

• Nutrition Research and Practice
• /
• 제8권2호
• /
• pp.220-226
• /
• 2014

BACKGROUND/OBJECTIVES: Arsenic, which causes human carcinogenicity, is ubiquitous in the environment. This study was designed to evaluate modulation of arsenic induced cancer by resveratrol, a phytoalexin found in vegetal dietary sources that has antioxidant and chemopreventive properties, in arsenic trioxide ($As_2O_3$)-induced Male Wistar rats. MATERIALS/METHODS: Adult rats received 3 mg/kg $As_2O_3$ (intravenous injection, iv.) on alternate days for 4 days. Resveratrol (8 mg/kg) was administered (iv.) 1 h before $As_2O_3$ treatment. The plasma and homogenization enzymes associated with oxidative stress of rat kidneys were measured, the kidneys were examined histologically and trace element contents were assessed. RESULTS: Rats treated with $As_2O_3$ had significantly higher oxidative stress and kidney arsenic accumulation; however, pretreatment with resveratrol reversed these changes. In addition, prior to treatment with resveratrol resulted in lower blood urea nitrogen, creatinine and insignificant renal tubular epithelial cell necrosis. Furthermore, the presence of resveratrol preserved the selenium content ($0.805{\pm}0.059{\mu}g/g$) of kidneys in rats treated with $As_2O_3$. However, resveratrol had no effect on zinc level in the kidney relative to $As_2O_3$-treated groups. CONCLUSIONS: Our data show that supplementation with resveratrol alleviated nephrotoxicity by improving antioxidant capacity and arsenic efflux. These findings suggest that resveratrol has the potential to protect against kidney damage in populations exposed to arsenic.

• 대한통합의학회지
• /
• 제8권4호
• /
• pp.163-169
• /
• 2020

Purpose : This study aimed to investigate the anti-allergic effect of Persicaria perfoliata water extract (PPWE) on IgE stimulated rat basophilic leukemia (RBL-2H3) cell line. Methods : P. perfoliata (L.) H. Gross has been used in traditional medicine as an anti-allergic agent, antipyretic, and diuretic and for respiratory disorders. To analyze the anti-allergic activity of PPWE, release of β-hexosaminidase in RBL-2H3 cells was estimated by enzyme linked immunosorbant assay (ELISA). Also, the cytotoxic effect of PPWE was identified by WST assay, and nuclear factor (NF)-κB and its upstream signaling molecules were assessed by western blot analysis. Results : PPWE treatment significantly attenuated β-hexosaminidase release in a dose dependent manner without any cytotoxicity. PPWE inhibited β-hexosaminidase activity by 38.4±1.2, 36.6±0.6, 32.5±2.2 and 26.5±1.2 at 500, 250, 100, and 50 ㎍/㎖ of PPWE, respectively, compared with the control group. In addition, an analysis of the expression level of NF-κB, an inflammation transcription factor, in RBL-2H3 cells upon IgE stimulation provided reults consistent with the results of β-hexosaminidase release. The phosphorylated status of upstream signaling molecules for transcription factor, mitogen activated protein kinases (MAPKs), was also analyzed. The results showed that PPWE treatment dose-dependently inhibited phosphorylation of extracellular regulatory kinase (ERK) and c-Jun N-terminal kinase (JNK). These results show that PPWE had a strong IgE-mediated degranulation inhibitory effect on RBL-2H3 cells. Conclusion : P. perfoliata ameliorated IgE-mediated allergic reaction via the modulation of MAPK and NF-κB signaling pathway in RBL-2H3 cells. These results indicate that P. perfoliata could be a potential candidate for a treatment strategy against various allergic disorders.