• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.508-514
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• 2001

A stuructural gene (ycl) encoding novel yeast cell wall hydrolase, YCL, was cloned from alkalophilic Bacillus alcalophilus subsp. YB380 by PCR, and transformed into E. coli JM83. Based on the N-terminal and internal amino acid sequences of the enzyme, primers were designed for PCr. The positive clone that harbors 1.8 kb of the yeast cell wall hydrolase gene was selected by the colony hybridization method with a PCR fragment as a probe. According to the computer analysis, this gene contained a 400-base-paired N-terminal domain of the enzyme. Based on nucletide homology of the cloned gene, a 850 bp fragment was amplified and the C-terminal domain of the enzyme was sequenced. With a combination of the two sequences, a full nucleotide sequence for YCL was obtained. This gene, ycl, consisted of 1,297 nucleotides with 27 nucleotides with 27 amino acids of signal sequence, 83 redundant amino acids of prosequence, and 265 amino acids of the mature protein. This gene was then cloned into the pJH27 shuttle vector and transformed into the Bacillus subtilis DB104 to express the enzyme. It was confirmed that the expressed cell wall hydrolase that was produced by Bacillus subtilis DB104 was the same as that of the donor strain, by Western blot using polyclonal antibody (IgY) prepared from White Leghorn hen. Purified yeast cell wall hydrolase and expressed recombinant protein showed a single band at the same position in the Western blot analysis.

• YAKHAK HOEJI
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• v.27 no.3
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• pp.185-205
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• 1983

Penicillins and cephalosporins are biosynthesized from L-.alpha.-aminoadipic acid, L-cysteine and L-valine. A tripeptide, LLD-$\delta$-($\alpha$-aminoadipyl)cysteinylvaline(LLD-ACV) was isolated from fermentation broths of Cephalosporium acremonium as well as of Penicillium chrysogenum and it was proved that the LL-$\delta$-($\alpha$-aminoadipyl cysteine was formed first in mycelia, to which valine would be connected to give LLD-ACV. However, several points are still unsolved; first, what mechanism is involved in the configurational change from L-valine to D-valine, second, what kind of cyclization mechanism gives a $\betha$-lactam ring and a thiazolidine ring and third, what is the pathways for the ring expansion from penicillins to cephalosporins. At present, it seems clear that LLD-ACV is cyclized to give isopenicillin N, which is transformed to penicillin N and further to cepbalosporin C. Other hydrophobic penicillins, including benzyl penicillin and penicillin V, are formed from isopenicillin N by acyl-exchange reactions catalyzed by penicillin transferase, rather than by acylation reaction on 6-aminopenicillanic acid(6-APA), which was isolated from the fermentation broth of P. chrysogenum and which would be formed by hydrolysis of $\delta-(\alpha$-amincadipyl)amido moiety at the C-6 position in isopenicillin N or penicillin N by penicillin acylase. Acylation of 6-APA is catalyzed also by penicillin acylase, but the reaction is proved not to be involved in penicillin biosynthesis. Understanding the biosynthesis of penicillins and cephalsoporins would provide solutions to increase in fermentation yields of penicillins, especially of cephalosporins and a solution to biological production of 7-aminocepbalosporanic acid (7-ACA) which is of importance in pharmaceutical industry. Still regulation mechanisms in penicillin and cephalosporin biosynthesis are unveiled at all.

• Korean Journal of Optics and Photonics
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• v.27 no.4
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• pp.133-142
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• 2016

This paper presents the design and simulation of a three-dimensional pixel-by-pixel scanning light detection and ranging (LIDAR) system with a microelectromechanical system (MEMS) scanning mirror and direct sequence optical code division multiple access (DS-OCDMA) techniques. It measures a frame with $848{\times}480$ pixels at a refresh rate of 60 fps. The emitted laser pulse waves of each pixel are coded with DS-OCDMA techniques. The coded laser pulse waves include the pixel's position in the frame, and a checksum. The LIDAR emits the coded laser pulse waves periodically, without idle listening time to receive returning light at the receiver. The MEMS scanning mirror is used to deflect and steer the coded laser pulse waves to a specific target point. When all the pixels in a frame have been processed, the travel time is used by the pixel-by-pixel scanning LIDAR to generate point cloud data as the measured result.

• Journal of the Korean Solar Energy Society
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• v.28 no.6
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• pp.78-86
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• 2008

The aim of this paper is to show the daylight environment of a light pipe system according to sun movement. A light pipe system has been mounted on the roof of the windowless full scale model: the solar spot has diameter of 0.65m and is 1.3m long, giving an aspect ratio of 1:2. The full scale model was installed on the rooftop of the SHINAN apartment in Yongin city that has no obstructions against sunlight. The test room is equipped with sensors for the measurements of the internal illuminance and has an area of 6m(W)$\times$6m(D)$\times$4m(H). The system has been monitored with a data-logger to evaluate the cumulative distribution of illuminance on a floor-plane from 16th, April to 29th, May, 2008 over one month and selected clear sky condition. For the daylight performance of floor area, the totally 49 measuring points has been used to determine the internal illuminance and an HP datalogger(HP34970A) records the measurements for one consecutive month. The horizontal external illuminance has been measured with two outdoor sensors. This paper presents the results of monitoring light pipe system with internal/external illuminance ratio and cumulative frequency distribution of floor-plane illuminance are discussed The results show that lightpipe is proficient device for introducing daylight into the building. However It provided different daylight indoor environment with wide or narrow Interquatile range of illuminance, internal/external illuminance ratio and cumulative frequency distribution according to solar positions under suuny sky condition. For more achieving the improvement of lightpipes also include energy savings, user visual comfort with various indicators; seasonal solar height, room and lightpipes geometries.

• Journal of Korean Society for Geospatial Information Science
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• v.14 no.3 s.37
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• pp.47-53
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• 2006

In this contribution, an integration of seamless navigation system for the pedestrian is introduced. To overcome the GPS outages in various situations, multi-sensor of GPS, INS, electronic barometer and compass are considered in one Extented Kalman filter. Especially, the integrated system is designed for low-cost for the practical applications. Therefore, a MEMS IMU is considered, and the low quality of the heading is compensated by the electronic compass. In addition, only the pseudoranges from GPS measurements are considered for possible real-time application so that the degraded height is also controlled by a barometer. The mathematical models for each sensor with systematic errors such as biases, scale factors are described in detail and the results are presented in terms of a covariance analysis as well as the position and attitude errors compared to the high-grade GPS/INS combined solutions. The real application scenario of GPS outage is also investigated to assess the feasible accuracy with respect to the outage period. The description on the current status of the development and future research directions are also stated.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.4
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• pp.893-902
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• 2016

The purpose of this study is to investigate the effect of wearing a weightlifting belt, which is an auxiliary equipment used during squat, by measuring and analyzing biomechanical difference in lower limb and proposing safer and to suggest a more effective exercise method for general population. Selected 8 male participants in their 20s who have not performed regular resistance exercise for at least a year, but have experience of performing squat. The comprehensive method of study is as follows: subjects were notified of the purpose of the study and were told to practice warm-up and the squat motion for the experiment for 20 minutes. When the participant believed they were ready to begin, the experiment was started. At controlled points, foot pressure distribution sensor has been installed. Then left and right feet have been placed on the pressure distribution sensor, from which data for successful squat position that does not satisfy the criteria for failure have been collected and computed with Kwon3D XP program and TPScan program. For data processing of this study, SPSS 21.0 was used to calculated mean (M) and standard deviation (SD) of the analyzed values, and paired t-test has been conducted to investigate the difference before and after wearing the weightlifting belt, with p-value of ${\alpha}<.05$. As for time consumed depending on usage of weightlifting belt in squat, statistically significant difference has been found in P2, which is recovery movement. Lower limb angle depending on usage of weightlifting belt in squat has shown statistically significant difference in E1 foot joint(p<. 001). There has been statistically significant difference in E2 knee joint. Foot pressure percentage depending on usage of weightlifting belt in squat were found to be statistically significant (p<. 01) in both regions of anterior and posterior foot.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.10
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• pp.64-76
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• 1998

In this paper, we propose the dynamic modeling, path planning and tracking algorithms of 4-wheeled 2-d.o.f.(degree of freedom) mobile robot(WMR). The gaussian functions are applied to design the smooth path of WMR. To calculate the WMR position in real time, we use three components of inertial measurement units(IMU). These units have initial error because of the rotation rate of earth, gravity acceleration and so on. Therefore we derive the initial error model of IMU, and compare the fitness diagnosis about probability characteristics of real data adn estimated data. The performance of IMU with error model and Kalman filter is compared to that without filter and error model. The simulation results show that the proposed dynamic model, path planning and tracking algorithms are more useful than the conventional control algorithm.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.53.2-53.2
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• 2014

IGRINS, the Immersion GRating INfrared Spectrometer, is a near-infrared wide-band high-resolution spectrograph jointly developed by the Korea Astronomy and Space Science Institute and the University of Texas at Austin. IGRINS employs three HAWAII-2RG focal plane array (FPA) detectors. The mechanical mounts for these detectors serves a critical function in the overall instrument design: Optically, they permit the only positional compensation in the otherwise "build to print" design. Thermally, they permit setting and control of the detector operating temperature independently of the cryostat bench. We present the design and fabrication of the mechanical mount as a single module. The detector mount includes the array housing, a housing for the SIDECAR ASIC, a field flattener lens holder, and a support base. The detector and ASIC housing will be kept at 65 K and the support base at 130 K. G10 supports thermally isolate the detector and ASIC housing from the support base. The field flattening lens holder attaches directly to the FPA array housing and holds the lens with a six-point kinematic mount. Fine adjustment features permit changes in axial position and in yaw and pitch angles. We optimized the structural stability and thermal characteristics of the mount design using computer-aided 3D modeling and finite element analysis. Based on the computer simulation, the designed detector mount meets the optical and thermal requirements very well.

• Steel and Composite Structures
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• v.27 no.6
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• pp.727-745
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• 2018

The design criteria and the corresponding performance levels characterize the response of extended stiffened end-plate beam-to-column joints. In order to guarantee a ductile behavior, hierarchy criteria should be adopted to enforce the plastic deformations in the ductile components of the joint. However, the effectiveness of thesecriteria can be impaired if the actual resistance of the end-plate material largely differs from the design value due to the potential activation of brittle failure modes of the bolt rows (e.g., occurrence of failure mode 3 in the place of mode 1 per bolt row). Also the number and the position of bolt rows directly affect the joint response. The presence of a bolt row in the center of the connection does not improve the strength of the joint under both gravity, wind and seismic loading, but it can modify the damage pattern of ductile connections, reducing the gap opening between the end-plate and the column face. On the other hand, the presence of a central bolt row can influence the capacity of the joint to resist the catenary actions developing under a column loss scenario, thus improving the joint robustness. Aiming at investigating the influence of these features on both the cyclic behavior and the response under column loss, a wide range of finite element analyses (FEAs) were performed and the main results are described and discussed in this paper.

• The Korean Journal of Rehabilitation Nursing
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• v.5 no.2
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• pp.113-123
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• 2002

The purpose of this study is to develop the standards of care in rehabilitation nurse specialist. This study was a descriptive survey. The data were collected 143 nurses who were worked in rehabilitation unit at 4 university hospital and 3 rehabilitation center in Korea from Aug. to Nov. 2000. The questionnaire was consisted of 78 items developed by the standards of care in rehabilitation nurse specialist in A.R.N. and the practice contents of care at rehabilitation unit in Korea. Collected data were analyzed by frequency, percentage, mean, S.D. ANOVA The results are as follows 1. For the adequency of the practice contents of rehabilitation nursing, area of skin care is the highest score, and safety, elimination, emotion, respiration, was ordered. 2. The adequency of the practice contents of rehabilitation nursing according to age, educational level, position, clinical experience, clinical experience in rehabilitation unit were significant difference.