• IMMUNE NETWORK
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• 제3권3호
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• pp.219-226
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• 2003

Background: Phage display is the most widely used technique among display methods to produce monoclonal antibody fragment with a specific binding activity. Having a large library for efficient antibody display/selection is quite laborious process to have more than $10^9$ members of transformants. To overcome these limitations, several in vitro selection approaches have been reported. Ribosome display that links phenotypes, proteins, directly to genotype, mRNA, is one of the in vitro display methods. Ribosome display can reach the size of scFv library up to $10^{14}$ molecules and it can be further diversified during PCR steps. To select the high affinity scFv from one pot library, we established ribosome display technique by modifying the previously reported eukaryotic translation system. Methods: To establish the antibody selection system by ribosome display, we used 3D8, anti-DNA antibody. A 3D8 scFv was synthesized in vitro by an in vitro transcription-translation system. The translated 3D8 scFv and the encoding 3D8 mRNA are connected to the ribosome. These scFv-ribosome-mRNA complexes were selected by binding to their specific antigens. The eluted mRNAs from the complexes are reverse transcribed and re-amplified by PCR. To apply this system, antibody library from immunized mouse with terminal protein (TP)-peptide of hepatitis B virus DNA polymerase TP domain was also used. This TP-peptide encompasses the 57~80 amino acid residues of TP. These mRNA/ribosome/scFv complexes by our system were panned three times against TP-peptide. The enrichment of antibody from library was determined by radioimmunoassay. Results: We specifically selected 3D8, anti-DNA antibody, against ssDNA as a model system. The selected 3D8 RNAs sequences from translation complexes were recovered by RT-PCR. By applying this model system, we enriched TP-peptide-specific scFv pools through three cycles of panning from immunized library. Conclusion: We show that our translating ribosome complexes are well maintained and we can enrich the TP-specific scFv pools. This system can be applied to select specific antibody from an antibody library.

• Journal of Information Display
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• 제11권2호
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• pp.76-83
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• 2010

This paper presents the multi-user autostereoscopic 3D display system constructed and operated by the authors using the time-multiplexing approach. This prototype has three main advantages over the previous versions developed by the authors: its hardware was simplified as only one optical array is used to create viewing regions in space, a lenticular multiplexing screen is not necessary as images can be produced sequentially on a fast 120Hz LCD with full resolution, and the holographic projector was replaced with a high-frame-rate digital micromirror device (DMD) projector. The whole system in this prototype consists of four major parts: a 120Hz high-frame-rate DMD projector, a 49-element optical array, a 120Hz screen assembly, and a multi-user head tracker. The display images for the left/right eyes are produced alternatively on a 120Hz direct-view LCD and are synchronized with the output of the projector, which acts as a backlight of the LCD. The novel steering optics controlled by the multiuser head tracker system directs the projector output to regions referred to as exit pupils, which are located in the viewers’eyes. The display can be developed in the "hang-on-the-wall"form.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.I
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• pp.46-49
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• 2005

In this paper, a novel 2D/3D convertible display system based on integral imaging is proposed. Combining two liquid crystal display panels with integral imaging, it is possible to convert the display between two-dimensional mode and three-dimensional mode without mechanical movement. The proposed method is proven by preliminary experiments.

• 한국게임학회 논문지
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• 제18권1호
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• pp.115-124
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• 2018

다수의 디스플레이 장치를 타일 형태로 구성하는 타일 기반 대규모 디스플레이 장치에 컴퓨터 게임과 같은 3D 어플리케이션을 표현하기 위해서는 일반적으로 이러한 디스플레이 장치에 적절한 어플리케이션을 별도로 개발하거나 이를 목적으로 특별히 개발된 API를 사용해야 한다. OpenGL, Direct3D 등의 레가시 3D API 기반의 원격 렌더링 기술을 확장하여 원격 분할 렌더링 시스템의 구현한다면 추가적인 개발 없이 타일 기반 대규모 디스플레이 장치에 3D 게임을 표현할 수 있다. 본 논문은 기 구현된 Direct3D 기반 원격 렌더링 시스템을 확장하여 원격 분할 렌더링 시스템을 구현한다. 이러한 원격 렌더링 시스템을 샘플 Direct3D 어플리케이션에 적용하여 몇가지 실험을 실시함으로써 기술적 실현 가능성을 확인한다.

• 한국통신학회:학술대회논문집
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• 한국통신학회 2004년도 하계종합학술발표회논문초록집
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• pp.48-48
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• 2004

• Journal of the Optical Society of Korea
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• 제18권3호
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• pp.225-229
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• 2014

A reflection-type three-dimensional (3D) screen using retroreflector is proposed to improve the visibility of a projected 3D image while keeping its perspective. For the projection-type 2D display, the diffuser is used to represent the 2D scene, overcoming the limitation of the aperture of the projection lens set. If the diffuser is adopted for the projected 3D image, only 2D images sectioned and blurred should be displayed on the screen. The proposed screen can make the 3D image with the aperture limitation visible to be applied to the 3D image projection systems. The feasibility of the proposed screen is verified by experiments.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 추계학술대회
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• pp.665-668
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• 2012

본 논문에서는 스마트폰이나 태블릿 PC등의 매체에서 2차원 적으로 보이는 컨텐츠를 프로젝션 도구를 사용해서 3차원 입체영상으로 볼 수 있게 하는 연구를 했다. 이를 위해 4장의 브라운 유리를 피라미드 모양으로 구성했고, PC로부터 출력되는 4대의 LCD 모니터 화면을 역 피라미드 모양의 거울 4면으로 각각 프로젝션 시켜서 거울의 중심부에 3차원 입체 영상이 나타나게 시스템을 구성했다. 실험은 태블릿 PC와 서버측 PC(데스크탑 PC)를 무선으로 연동시켜, 태블릿 PC에서 선택한 콘텐츠가 서버측 PC에서 실행되어 역 피라미드형 거울의 중심부에 3차원 입체영상으로 보이게 했다. 실험결과 관찰자의 관찰 각도에 따라서 조금 차이가 나지만 2차원 컨텐츠를 3차원적으로 디스플레이 하는 것이 가능함을 보였다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2000년도 제1회 학술대회 논문집
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• pp.181-182
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• 2000

The glass compositions of $PbO-SiO_2-B_2O_3$ system and $P_2O_5-PbO-ZnO$ system for the transparent dielectric materials for front panel and $P_2O_5-ZnO-BaO$ and $SiO_2-ZnO-B_2O_3$ for the reflective dielectric materials for back panel of PDP(Plasma Display Panel) were investigated. As a transparent dielectric materials for front panel, $PbO-SiO_2-B_2O_3$ glass showed good dielectric properties, high transparency and proper thermal expansion matching to soda-lime glass substrate. And the reflective dielectric materials for back panel were prepared from parent glass of $SiO_2-ZnO-B_2O_3$ system and oxide filler. It was found that these glass-ceramics are useful materials for reflective dielectric layers, as those have a similar thermal expansion to soda-lime glass plate, high reflectance, low sintering temperature.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.1033-1036
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• 2008

In this paper, we describe the development of the polarized-light stereoscopic monitoring system based on full HDTV. The system consists of the stereoscopic camera part, image processing device for the stereoscopic image recording and stereoscopic display. The developed stereoscopic imaging display system reduces eyestrain and viewer fatigue.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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• pp.86-91
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• 2006

This paper introduces two systems for editing and evaluating 3D content. The systems, a non-linear editing system and an evaluation system, were developed to improve viewing safety and comfort for 3D content observers and provide better usability for the creators from the viewpoint of ergonomics.