• 환경생물
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• 제34권2호
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• pp.107-115
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• 2016

전 세계적으로 원자력 발전소는 442기가 가동 중이며, 62기가 충원될 예정이다. 원자력 발전소의 증가에 따라 방사성 폐기물 유출에 대한 위험성도 증가하였다. 이러한 이유 때문에, 방사성 폐기물의 처리는 인간, 동물, 식물을 포함하는 자연 생태계를 보전하는 관점에서 중요하다. 또한, 방사성 폐기물 유출은 그 지역뿐만 아니라 전 세계적으로 심각한 문제를 야기한다. 본 연구는 입체 배양세포에 방사성 핵종원소(세슘, 스트론튬, 코발트)를 처리하였고 이에 대한 영향력을 확인하였다. 입체 배양 구조체는 아가로오스 하이드로겔을 이용하여 제작했으며 암세포 및 정상세포 (HeLa, HepG2, COS-7)를 사용하여 입체 배양을 실시 하였다. 입체 형태로 세포를 배양한 후 세슘, 스트론튬, 코발트 농도 변화에 따라 세포 생존능력을 분석하였다. 이때 입체 배양세포에서 생존능력이 단층 배양세포 보다 최대 42% 우수한 것을 확인하였다. 입체 배양구조체는 세포가 형태 및 생리학적으로 in vivo환경인 조직과 비슷하게 배양을 가능하게 하였다. 따라서, 입체 배양구조체는 기존의 단층 배양 한계점인 in vivo 환경에 적용시킬 수 없다는 한계를 극복하였다. 본 입체 배양 기술이 중금속 독성평가 및 단시간 내에 다수의 물질 분석을 수행하는 고속 대량 스크리닝 기술에 활용될 것으로 기대한다.

• 복식문화연구
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• 제19권2호
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• pp.316-323
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• 2011

This research aim concerns questioning how we can generate environments suggestive of nature fused with built environments through textiles. Through literature reviews and experiments with available the 3D imaging techniques of Holography, Lenticular and other new technologies. We also have researched towards finding the most effective method for 3D imaging techniques for textile applications. The advantage of the combining technique is to create the possibility of seeing a number of different floating 3D illusory images, depending on the viewing angle. This objective is to produce intriguing textile patterns and images in which the objects and colours change as viewpoints change. Experimental work was carried out in collaboration with professional textile researchers, scientists, artists and designers conducting research in this field.

• International Journal of Advanced Culture Technology
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• 제2권1호
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• pp.30-32
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• 2014

3D printing technology polymeric material or plastic and metallic powder to suit the drafting of additive manufacturing would gradually products soars. 3D printing technologyapplication of a wide variety of industrial sectors. 3D printing technology enables raw materials consumption is less, the supply chain are shorter depending on the load and reduce the use of fossil fuels.Emergence of 3D printing technology so called the third industrial revolution in ICT market, quickly spread worldwide.In the future, 3D printing technology is simply beyond bio-technology, Nano-engineering, the manufacture of the product, incorporating a variety of technologies to improve the quality of life of human beings have played an important role will be.

• 한국환경과학회지
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• 제8권5호
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• pp.575-586
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• 1999

From the environmental aspects, primary productivity of phytoplankton plays the most improtant role in enhancement of marine culture oyster production. This study may be divided into two branches; one is estimation of maximum oyster meat production per unit facility(Carrying Capacity) under the present enviromental conditions in Kamak Bay, the other is improvement of carrying from increase of primary productivity by changing the environmental conditions that cause not ot form an unfavorable environment such as the formation of oxygen deficient water mass using the eco-hydrodynamic model. By simulation of three-dimensional hydrdynamic model and ecosystem model, the comparison between observed and computed data showed good agreement. The results of sensitivity analysis showed that phytoplankton maximum growth rate was the most important parameter for phytoplankton and dissolved oxygen. The estimation of mean primary productivity of Wonpo, Kamak, Pyongsa, and Kunnae culture grounds in Kamak Bay during culturing period were 3.73gC/$m^2$/d, 2.12gC/$m^2$/d, 1.98gC/$m^2$/d, and 1.26gC/$m^2$/d, respectively. Under condition not ot form the oxygen deficient water mass, four times increasing of pollutants loading as much as the present loading from river increased mean primary productivity of whole culture grounds to 4.02gC/$m^2$/d. Sediment N, P fluxes that allowed for 35% increasing from the present conditions increased mean primary productivity of whole culture grounds to 3.65gC/$m^2$/d. Finally, ten times increasing of boundary loadings from the present conditions increased mean primary productivity of whole culture grounds to 3.95gC/$m^2$/d. The maximum oyster meat production per year and that of unit facility in actual oyster culture grounds under the present conditions were 6,929ton and 0.93ton, respectively. This 0.93ton/unit facility is considered to be the carrying capacity in study area, and if the primary productivity is increased by changing the environmental conditions, oyster production can be increased.

• Reproductive and Developmental Biology
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• 제36권1호
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.580-585
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• 1997

We selected two mutants namely strain D5 and Nu23 by mutagenesis of anthracycline producing Streptomyces: the former is an $\varepsilon$-rhodomycinone overproducing mutant selected from Streptomyces sp. C5, a baumycin producer and the latter, a blocked mutant of early pathway for doxorubicin biosynthesis obtained from Streptomyces peucetius ATCC 27952, a doxorubicin producer. The mutant strain Nu23 does not produce anthracycline metabolites but retains the most of enzyme activities converting aklavinone to doxorubicin and the mutant strain D5 produced $\varepsilon$-rhodomycinone at a level of 150 $\mu$g/ml. These strains were grown separately in NDYE medium and each was mixed at day 3 by equal volume of culture broth but the quantity of doxorubicin produced was far below an estimation based on the level of $\varepsilon$-rhodomycinone normaly produced by the strain D5. On the other hand doxorubicin was reached at maximum level after 4 days in the mixed culture condition which was composed of culture broth of strain D5 grown for 6 day and that of strain Nu23 grown for 3 day. It was turned out that the growth of mutant strain D5 was inhibited by the accumulation of daunorubicin and doxorubicin in mixed culture broth, which cause the limitation of $\varepsilon$-rhodomycinone.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.408-413
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• 2006

We assessed the ability of a Pseudonocardia sp. from soil samples to bioconvert vitamin $D_3$. The optimal culture conditions for the bioconversion of vitamin $D_3$ to active $1{\alpha}$,25-dihydroxyvitamin $D_3$ were investigated by varying the carbon and nitrogen sources, the metal salt concentrations, the initial pH, and the temperature. Microbial transformations were carried out with the addition of vitamin $D_3$ dissolved in ethanol. They were sampled by extraction with methanol-dichloromethane and the samples were examined by HPLC. Optimum culture conditions were found to be 0.4% yeast extract, 1% glucose, 3% starch, 1% fish meal, 0.2% NaCl, 0.01% $K_2HPO_4$, 0.2% $CaCO_3$, 0.01% NaF, and pH 7.0 at $28^{\circ}C$. The optimal timing of the addition of vitamin $D_3$ for the production of calcitriol by Pseudonocardia autotrophica ID9302 was concurrent with the inoculation of seed culture broth. Maximum calcitriol productivity and the yield of bioconversion reached a value of 10.4mg/L and 10.4% respectively on the 7th day in a 75L fementer jar under the above conditions.

• 문화기술의 융합
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• 제7권1호
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• pp.75-81
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• 2021

첨단 정보통신 기술의 발달에 따라 디지털 문화에 익숙한 Z세대는 미래 패션을 재편할 수 있는 패션 산업의 주요 소비층으로 관심이 집중되고 있다. 본 연구에서는 디지털 기기에 능숙하고 인터넷을 통한 정보 습득 및 모바일 라이프스타일에 친숙한 Z세대를 위한 캐쥬얼웨어를 3D 가상 시뮬레이션에 의해 디자인하여 걸리쉬, 스포티브, 이지, 컨템포러리의 4가지 룩으로 디자인 전개하였다. 의상디자인에 3D 가상 착의 시뮬레이션의 활용은 팬데믹 환경에서 디지털 패션 디자인기획 및 생산 프로세스와의 융합을 통한 미래 패션 산업의 디지털화를 구축하고 온라인 플랫폼의 유통을 강화시킬 수 있다. 언택트 패션 제작 시스템을 도입하여 업무의 효율성 강화를 위한 지속적인 혁신을 시도하고 있는 기업 환경에서 3D 가상의상 기술의 활용은 3D 샘플 제작, 온라인 피팅, 수정과 보정 및 품평을 통해 업무 프로세스에서 소통이 확대되고 인적, 물적 자원의 경감에 따른 긍정적 효과를 통해 지속 가능한 패션의 가치를 확대시킬 수 있다.

• 복식문화연구
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• 제18권6호
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• pp.1305-1317
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• 2010

본 연구는 다양한 3D imaging 기술이 생산해낼 수 있는 환영효과를 직물에 적용할 수 있는지에 대한 가능성을 통해서, 현실과 허구의 경계가 없어지는 새로운 환경을 조성하기 위한 직물을 개발하여 변형 가능한 삼차원의 살아있는 직물 같은, 관점에 따라 패턴과 색깔의 이미지가 바뀌는 흥미로운 직물패턴의 실현 가능성을 알아본다. 본 논문은 I, II로 나뉘어 있으며, 각 논문에서 각기 다른 실험을 실시하여 결과로의 적용가능성과 제한점을 살펴봄으로써 Holography, Lenticular, 등 가상의 3D 테크놀로지를 통해 2D 평면의 구조에 3D 가상 이미지의 텍스타일 적용 가능성을 기계적 실험을 통해 확인하며, 3D imaging 기술에 대한 경험과 이해를 얻고, 3D imaging 기술을 적용할 수 있는 잠재력을 연구하기 위한 것으로, 실험은 현장에 있는 전문 텍스타일연구가, 과학자, 예술가 그리고 디자이너들의 협업으로 이루어졌다.

• 한국의류산업학회지
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• 제21권4호
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• pp.412-419
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• 2019

This study is to help maintain the identity of traditional Korean culture and develop fashion culture products by applying images and composition forms of traditional lattice patterns for vest designs. Lattice can be distinguished as a shape created through intersecting vertical and horizontal lines, in the form of oblique lines in vertical and horizontal structures, and in the form of an oblique line. Lattice patterns represent the overall unity and order as well as the beauty of small spaces created by simple lines. Traditional lattice patterns of the Joseon Dynasty were examined theoretically through the literature. Based on theoretical grounds, there is study illustrates vest designs using Adobe Illustrator CS6 as well as works on a 3D virtual costume using CLO Trial 4.2, a 3D virtual dressing system of CLO Virtual Fashion. This study developed an easy to wear vest design categorized as clothes for both men and women. The geometric formality of the lattice pattern has been applied to fashion culture products. In this study, the design was developed focusing on lattice, kotsal (flower pattern), sosulbitsal (diagonal), sotdaesal, tisal (horizontal & vertical). This study can be used as basic data in the domestic fashion cultural product market that can help maintain the originality of Korean culture in the global era that also assists in the successful promotion of Korean culture and traditions.