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Optimization of Culture Conditions for the Bioconversion of Vitamin $D_3\;to\;1{\alpha}$,25-Dihydroxyvitamin $D_3$ Using Pseudonocardia autotrophica ID9302  

Kang, Dae-Jung (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
Lee, Hong-Sub (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
Park, Joon-Tae (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
Bang, Ji-Sun (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
Hong, Soon-Kwang (Department of Biological Science, Myongji Unviersity)
Kim, Tae-Yong (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.5, 2006 , pp. 408-413 More about this Journal
Abstract
We assessed the ability of a Pseudonocardia sp. from soil samples to bioconvert vitamin $D_3$. The optimal culture conditions for the bioconversion of vitamin $D_3$ to active $1{\alpha}$,25-dihydroxyvitamin $D_3$ were investigated by varying the carbon and nitrogen sources, the metal salt concentrations, the initial pH, and the temperature. Microbial transformations were carried out with the addition of vitamin $D_3$ dissolved in ethanol. They were sampled by extraction with methanol-dichloromethane and the samples were examined by HPLC. Optimum culture conditions were found to be 0.4% yeast extract, 1% glucose, 3% starch, 1% fish meal, 0.2% NaCl, 0.01% $K_2HPO_4$, 0.2% $CaCO_3$, 0.01% NaF, and pH 7.0 at $28^{\circ}C$. The optimal timing of the addition of vitamin $D_3$ for the production of calcitriol by Pseudonocardia autotrophica ID9302 was concurrent with the inoculation of seed culture broth. Maximum calcitriol productivity and the yield of bioconversion reached a value of 10.4mg/L and 10.4% respectively on the 7th day in a 75L fementer jar under the above conditions.
Keywords
bioconversion; calcitriol; calcifediol; Pseudonocardia autotrophica ID9302; fermentation;
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