• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.144.1-144
• /
• 2003

Attenuated viruses can protect their hosts against challenge to their related viruses. Increasing evidence shows that mutations of the tobamoviral 126/183 kDa protein play a major role in the viral attenuation and contribute to the cross protection mechanism. In this study, four mutants of Pepper mild mottle virus (PMMoV) have been constructed by mutagenesis; two mutants, pTPpoly348 and pTPpoly762, were substituted in the middle of replicase gene, and the others, pTPL3D:: $\Delta$6207 and pTPL3D:: $\Delta$6219, were deletion mutants made by deleting some parts of pseudoknot structures of the 3' noncoding region (NCR) of the virus. Progeny viruses generated from the four mutants were infectious on N. benthamiana plants with symptomless or mild mosaic symptom. Replication efficiency and viral product accumulations of four mutants were assessed by Northern and Western blot analyses on BY-2 protoplast cells. Accumulation of CP for the pTPL3D:: $\Delta$6207 and pTPL3D:: $\Delta$6219 were lower than that of other mutants and wild type virus. These data suggest that the 3'-NCR mutations contribute to the viral gene expression in host tissues, while mutants of replicase gene rather govern the symptom expression.

• 한국안전학회지
• /
• 제33권3호
• /
• pp.1-7
• /
• 2018

Recently, various engineering approaches have been widely used in the accident investigation field to identify the cause of the accident and to predict damage by accident. Computational analysis is the most commonly used method of accident investigation technique. This technique is mainly used to identify the mechanism of the accident generation and to determine the cause when it is difficult to reproduce the situation at the time of the accident or when it is impossible to perform a reproduction experiment. In this study, The gas explosion analysis for LPG explosion accident generated by defect of the blocking action was performed to determine the accident object, gas leakage amount and predicted the damage caused by the accident using 3D laser scanner and FLACS program. We can quantify the explosive power by LPG gas accident and predict the gas leakage amount, damage by accident and evaluate the stability of the structure through this study. In the future, This method can be widely used in the field of gas safety by improving the reliability and validity of the analysis.

• International Journal of Advanced Culture Technology
• /
• 제8권3호
• /
• pp.260-268
• /
• 2020

Currently, it is usually a 3D scanner processing method as a laser method. However, the laser method has a disadvantage of slow scanning speed and poor precision. Although optical scanners are used as a method to compensate for these shortcomings, optical scanners are closely related to the distance and precision of the object, and have the disadvantage of being expensive. In this paper, 3D scanner using variable focus lens-based structured light module with improved measurement precision was designed to be high performance, low price, and usable in industrial fields. To this end, designed a telecentric optical system based on a variable focus lens and connected to the telecentric mechanism of the step motor and lens to adjust the focus of the variable lens. Designed a connection structure with optimized scalability of hardware circuits that configures a stepper motor to form a system with a built-in processor. In addition, by applying an algorithm that can simultaneously acquire high-resolution texture image and depth information and apply image synthesis technology and GPU-based high-speed structured light processing technology, it is also stable for changes to external light. We will designed and implemented for further improving high measurement precision.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
• /
• 제9권6호
• /
• pp.623-634
• /
• 2003

본 논문에서는 시간 편집 환경과 공간 편집 환경을 하나의 삼차원 저작 환경으로 통합한 멀티미디어 프레젠테이션 공동 저작 시스템을 소개한다. 본 저작 시스템은 하나의 삼차원 좌표계에서 멀티미디어 프레젠테이션의 공간 속성과 시간 속성을 동시에 표현한다. 한 개의 축은 일반적인 시간 정보(T-zone)를 표현하고 나머지 두개의 축은 공간 정보(XY-zone)를 나타내며 시간적인 미디어 객체들은 삼차원 육면체로 표현하고 오디오 객체는 원통 모양으로 표현하고 있으므로, 사용자들이 자유자제로 삼차원공간을 이동하며 시간 편집과 공간 편집을 할 수 있어 효율적이다. 또한 본 논문에서는 제안하는 공동 저작 시스템에서 생성되는 공유 객체들에 대한 효율적인 동시성 제어 기법을 설계하고 구현하였다. 구현된 동시성 제어 기법은 다중버전 기법(multiple versions)을 가시화하여 공동 저작에서의 사용자 인지(user awareness)를 높였으며 공유 객체에 대한 접근허가권(access permissions)을 이용하여 네트워크 통신에서의 지연이나 장애로 발생할 수 있는 충돌(collision)을 최소화하였다. 그리고 이 동시성 제어 기법은 잠금 단위를 세밀하게 하여 사용자의 편집 자유도를 최대한 보장하였으며, 공유 객체의 일관성을 유지하면서 낙관적 동시성 제어(optimistic concurrency control)를 지원하므로 사용자들에게 멀티미디어 프레젠테이션을 동시 편집할 때 빠른 응답 시간을 제공할 수 있다.

• Geomechanics and Engineering
• /
• 제20권1호
• /
• pp.43-54
• /
• 2020

The limit analysis and response surfaces method were combined to investigate the reliability of pressurized tunnel faces subjected to seismic force. The quasi-static method was utilized to introduce seismic force into the tunnel face. A 3D horn failure mechanism of pressurized tunnel faces subjected to seismic force was constructed. The collapse pressure of pressurized tunnel faces was solved by the kinematical approach. The limit state equation of pressurized tunnel faces was obtained according to the collapse pressure and support pressure. And then a reliability model of pressurized tunnel faces was established. The feasibility and superiority of the response surfaces method was verified by comparing with the Monte Carlo method. The influence of the mean of soil parameters and support pressure, variation coefficients, distribution type and correlation of c-φ on the reliability of pressurized tunnel faces was discussed. The reasonable safety factor and support pressure required by pressurized tunnel faces to satisfy 3 safety levels were presented. In addition, the effects of horizontal seismic force, vertical seismic force and correlation of k_h-k_v on the reliability of pressurized tunnel faces were also performed. The method of this work can give a new idea for anti-seismic design of pressurized tunnel faces.

• 한국식품영양과학회지
• /
• 제23권3호
• /
• pp.443-452
• /
• 1994

This study describes the effects of novel1, 25-dihydroxyvitamin D$_3$ analong[1,25(OH)$_2$-16ene-23yne-26, 27-F6-D$_3$] on proliferation of the human histiocytic lymphoma cell line U937 in vitro. We also examined the expression of c-myc oncogene in U937 cells was apparently inhibited to 62% and 87% of the control level after 4 days in the presence of 10-8M and 10-7 M of this analog, respectively. This compound morpholgically and functionally differentiated U937 cells to nonocyte-macrophage phenotype showing the increase of adherence ability to surface and a decrease of N/C ratio in Giemsa staining . Especially, nonspecific esterase activity which is a marker of cell differentiation to monocyte-macrophage was positive, and production of the positive stained cells increased in a dose dependent fashion . The expression of c-myc oncogene by 1, 25(OH)$_2$D$_3$ analog(10-7 M) was reduced by 60% at the mRNA level as determined by Northern blotting. The effects of this novel analog on cell proliferation and cell differentiation may open op new therapeutic strategies for human disorders such as psoriassis and may provide a tool to understand the mechanism of action of vitamin D$_3$ seco-steroids in malignancy.

• BMB Reports
• /
• 제55권9호
• /
• pp.447-452
• /
• 2022

Neurogenic differentiation 1 (NeuroD1) is an essential transcription factor for neuronal differentiation, maturation, and survival, and is associated with inflammation in lipopolysaccharide (LPS)-induced glial cells; however, the concrete mechanisms are still ambiguous. Therefore, we investigated whether NeuroD1-targeting miRNAs affect inflammation and neuronal apoptosis, as well as the underlying mechanism. First, we confirmed that miR-30a-5p and miR-153-3p, which target NeuroD1, reduced NeuroD1 expression in microglia and astrocytes. In LPS-induced microglia, miR-30a-5p and miR-153-3p suppressed pro-inflammatory cytokines, reactive oxygen species, the phosphorylation of c-Jun N-terminal kinase, extracellular-signal-regulated kinase (ERK), and p38, and the expression of cyclooxygenase and inducible nitric oxide synthase (iNOS) via the NF-κB pathway. Moreover, miR-30a-5p and miR-153-3p inhibited the expression of NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasomes, NLRP3, cleaved caspase-1, and IL-1β, which are involved in the innate immune response. In LPS-induced astrocytes, miR-30a-5p and miR-153-3p reduced ERK phosphorylation and iNOS expression via the STAT-3 pathway. Notably, miR-30a-5p exerted greater anti-inflammatory effects than miR-153-3p. Together, these results indicate that miR-30a-5p and miR-153-3p inhibit MAPK/NF-κB pathway in microglia as well as ERK/STAT-3 pathway in astrocytes to reduce LPS-induced neuronal apoptosis. This study highlights the importance of NeuroD1 in microglia and astrocytes neuroinflammation and suggests that it can be regulated by miR-30a-5p and miR-153-3p.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
• /
• pp.344.1-344.1
• /
• 2002

We report the synthesis of several new 3-aminohydantoinyl-1.2-benzothiazine derivatives and propose an another mechanism of the cyclization to the hydantoins for the development candidates of COX-2 inhibitors. 3-Aminohydantoins 3a-d were prepared through cyclization of the condensation products that were formed by heating amino acids and tert-bytyl carbazate in quinoline according to the method of Lalezari. (omitted)

• 한국운동역학회지
• /
• 제31권3호
• /
• pp.155-161
• /
• 2021

Objective: There is a lack of studies using the 3D-2D image registration techniques on the mechanism of a shoulder injury for ice hockey players. This study aimed to analyze in vivo 3D glenohumeral joint arthrokinematics in collegiate ice hockey athletes and compare shoulder scaption with or without a hockey stick using the 3D-2D image registration technique. Method: We recruited 12 male elite ice hockey players (age, 19.88 ± 0.65 years). For arthrokinematic analysis of the common shoulder abduction movements of the injury pathogenesis of ice hockey players, participants abducted their dominant arm along the scapular plane and then grabbed a stick using the same motion under C-arm fluoroscopy with 16 frames per second. Computed tomography (CT) scans of the shoulder complex were obtained with a 0.6-mm slice pitch. Data from the humerus translation distances, scapula upward rotation, anterior-posterior tilt, internal to external rotation angles, and scapulohumeral rhythm (SHR) ratio on glenohumeral (GH) joint kinematics were outputted using a MATLAB customized code. Results: The humeral translation in the stick hand compared to the bare hand moved more anterior and more superior until the abduction angle reached 40°. When the GH joint in the stick hand was at the maximal abduction of the scapula, the scapula was externally rotated 2~5° relative to 0°. The SHR ratio relative to the abduction along the scapular plane at 40° indicated a statistically significant difference between the two groups (p < 0.05). Conclusion: With arm loading with the stick, the humeral and scapular kinematics showed a significant correlation in the initial section of the SHR. Although these correlations might be difficult in clinical settings, ice hockey athletes can lead to the movement difference of the scapulohumeral joints with inherent instability.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제13권3호
• /
• pp.1311-1324
• /
• 2019

In the traditional cellular network communication, the cellular user and the base station exchange information through the uplink channel and downlink channel. Meanwhile, device-to-device (D2D) users access the cellular network by reusing the channel resources of the cellular users. However, when cellular user channel conditions are poor, not only D2D user cannot reuse its channel resources to access the network, but also cellular user's communication needs cannot be met. To solve this problem, we introduced a novelty D2D communication mechanism in the downlink, which D2D transmitter users as half-duplex (HD) relays to assist the downlink transmission of cellular users with reusing corresponding spectrum. The optimization goal of the system is to make the cellular users in the bad channel state meet the minimum transmission rate requirement and at the same time maximize the throughput of the D2D users. In addition, i for the purpose of improving the efficiency of relay transmission, we use two-antenna architecture of D2D relay to enable receive and transmit signals at the same time. Then we optimized power of base station and D2D relay separately with consideration of backhaul interference caused by two-antenna architectures. The simulation results show that the proposed HD relay strategyis superior to existing HD and full-duplex (FD) models in the aspects of system throughput and power efficiency.