• Analytical Science and Technology
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• v.36 no.4
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• pp.143-151
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• 2023

Considering the greater role of α-amino acids in our daily lives, the enantiomer resolution of seven α-amino acids derivatized with fluorogenic reagent (4-fluoro-7-nitro-2,1,3-benzoxadiazole, NBD-F) by chiral HPLC on amylose or cellulose trisphenylcarbamate derived chiral stationary phases (CSPs) under simultaneous ultraviolet (UV) and fluorescence (FL) detection was performed. The degree of enantioseparation and resolution was affected by nature and selector backbones of the CSPs as well as the kind of amino acids. Baseline enantiomer separation and resolutions were observed for the enantiomers of all analytes as NBD derivatives especially on coated type amylose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak AD-H and Lux Amylose-1). The other CSPs also showed good enantioselectivity except for the CSPs (Chiralpak IB, Chiralcel OD-H and Lux Cellulose-1) having cellulose tris(3,5-dimethylphenylcarbamate) as chiral selectors. The developed analytical chiral method was applied to determine the enantiomeric purity of seven commercially available L-α-amino acids and the impurities as D-forms were found to be in the range 0.08-0.87 %, respectively. The intra- and interday accuracy and precision assays showed high accuracy and precision of the developed analytical method. This chiral HPLC method for the enantiomer resolution of amino acids using fluorescent derivatization could be useful for the determination of enantiomeric purity of pharmaceuticals and biological study for amino acid type compounds among chiral drugs.

• Structural Engineering and Mechanics
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• v.55 no.4
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• pp.801-813
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• 2015

Flexural stiffness of bridge spans has become even more important parameter since Eurocode 1 introduced for railway bridges the serviceability limit state of resonance. For simply supported bridge spans it relies, in general, on accurate assessment of span moment of inertia that governs span flexural stiffness. The paper presents three methods of estimation of the equivalent moment of inertia for such spans: experimental, analytical and numerical. Test loading of the twin truss bridge spans and test results are presented. Recorded displacements and the method of least squares are used to find an "experimental" moment of inertia. Then it is computed according to the analytical method that accounts for joint action of truss girders and composite deck as well as limited span shear stiffness provided by diagonal bracing. Finally a 3D model of finite element method is created to assess the moment of inertia. Discussion of results is given. The comparative analysis proves efficiency of the analytical method.

• Archives of Pharmacal Research
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• v.5 no.2
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• pp.87-91
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• 1982

In order to develop an analytical method for amikacin and butirosin in presence of their parent antibiotics, kanamycin A and ribostamycin, high-performance liquid chromatographic technique and microbioassay method were evaluated and compared. Using high performance liquid chromatography, two acylated antibiotics, amikacin and butirosin was partially separated from their parent antibiotics, to provide a qualitative analytical method. In microbioassay using Pseudomonas aeruginosa TI-13, a producer of aminoglycoside-3-phosphotransferase I, only acylated antibiotics were selectively analyzed when paper disc-susceptibility assay was used. The standard curve showed a good correlation between the response and odse in semilogarithmic plat with correlation coefficients above 0.96, and analytical deviation from expected dose was within 10%.

• Clean Technology
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• v.28 no.4
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• pp.323-330
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• 2022

Microfluidic paper-based analytical devices (μPADs) have recently been in the spotlight for their applicability in point-of-care diagnostics and environmental material detection. This study presents a double-sided printing method for fabricating 3D-μPADs, providing simple and cost effective metal ion detection. The design of the 3D-μPAD was made into an acryl stamp by laser cutting and then coating it with a thin layer of PDMS using the spin-coating method. This fabricated stamp was used to form the 3D structure of the hydrophobic barrier through a double-sided contact printing method. The fabrication of the 3D hydrophobic barrier within a single sheet was optimized by controlling the spin-coating rate, reagent ratio and contacting time. The optimal conditions were found by analyzing the area change of the PDMS hydrophobic barrier and hydrophilic channel using ink with chromatography paper. Using the fabricated 3D-μPAD under optimized conditions, Ni²⁺, Cu²⁺, Hg²⁺, and pH were detected at different concentrations and displayed with color intensity in grayscale for quantitative analysis using ImageJ. This study demonstrated that a 3D-μPAD biosensor can be applied to detect metal ions without special analysis equipment. This 3D-μPAD provides a highly portable and rapid on-site monitoring platform for detecting multiple heavy metal ions with extremely high repeatability, which is useful for resource-limited areas and developing countries.

• International Journal of Naval Architecture and Ocean Engineering
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• v.13 no.1
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• pp.50-56
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• 2021

An accurate evaluation of three-dimensional (3D) Time-Domain Green Function (TDGF) in infinite water depth is essential for ship's hydrodynamic analysis. Various numerical algorithms based on the TDGF properties are considered, including the ascending series expansion at small time parameter, the asymptotic expansion at large time parameter and the Taylor series expansion combines with ordinary differential equation for the time domain analysis. An efficient method (referred as "Present Method") for a better accuracy evaluation of TDGF has been proposed. The numerical results generated from precise integration method and analytical solution of Shan et al. (2019) revealed that the "Present method" provides a better solution in the computational domain. The comparison of the heave hydrodynamic coefficients in solving the radiation problem of a hemisphere at zero speed between the "Present method" and the analytical solutions proposed by Hulme (1982) showed that the difference of result is small, less than 3%.

• Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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• 2000.02a
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• pp.60-62
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• 2000

It is widely acknowledged that the value of critical current in High Temperature Superconducting (HTS) tape has a great influence on B. Therefore, when HTS cable is designed, a method to reduce the B should be considered in order to improve the capacity. This paper deals with the influence of the space between each HTS tape by using 2D analytical method. From the analysis results, it is found that the decrease of the space causes the decrease of B. Moreover, another HTS cable, which has a very small space, is analyzed by 3D analytical method and it is manufactured. The validity of these analysis results are verified by comparison with experimental results.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.35D no.3
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• pp.46-55
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• 1998

This stud demonstrates the profiles of heavy ions (As, Sb) in silicon by high energy (1~10 MeV) implantation. Implanted profiles were measured by SIMS (Cameca 4f) and compared with simulation results (TRIM) program and analytical description method using Pearson function). The experimental results have a little bit deviation with simulation data in the case of As high energy implatation. But in the case of Sb, the experimental results are in good agreement with TRIM data. SIMS profiles are perfectly fitted with a analytical description method only using one pearson function in Sb implantation. but in the case of As, fitted profilesshow with a little bit deviations by channeling effects of SIMS profiles. Thermal annealing for electrical activation of implanted ions was carried out by furnace annealing and RTA(Rapid Thermal Annealing). Concentration-depth profile after heat treatement were measured by SR(Spreading Resistance) method.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.4 no.2
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• pp.153-163
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• 2000

The non-dimensional convective heat losses from each surface are investigated as a function of the non-dimensional fin length, width and the ratio of upper surface Biot number to bottom surface Biot number (Bi2/Bi1) using the three-dimensional separation of variables method. Heat loss ratio in view of each surface with the variation of Bi2/Bi1 is presented. The variation of the non-dimensioal temperare profile along the fin center line for a thermally asymmetric conditions is also presented.

• Journal of Mechanical Science and Technology
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• v.15 no.11
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• pp.1541-1547
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• 2001

The non-dimensional fin length for optimum heat loss from a thermally asymmetric rectangular fin is represented as a function of the ratio of the bottom surface Biot number to the top surface Biot number, fin tip surface Biot number and the non-dimensional fin width. Optimum heat loss is taken as 98% of the maximum heat loss. For this analysis, three dimensional separation of variables method is used. Also, the relation between the ratio of the bottom surface Biot number to the top surface Biot number and the ratio of the right surface Biot number to the left surface Biot number is presented.

• Journal of the Korean Society for Advanced Composite Structures
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• v.6 no.2
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• pp.77-84
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• 2015

The purpose of this study was the analytical safety evaluation on the super-structure of precast modular bridge using standardized modular members and robotic construction during the transportation routing and lifting conditions. In order to evaluate the safety performance of the bridge system, 3-D full scale Finite Element (FE) of 40 m standardized modular block was developed in ABAQUS, followed by the analytical study to classify the structural system according to steel girder structures: 1) modular bridge block lifting method including the steel girder system; 2) modular bridge block lifting method without the steel girder system. The results from the analytical study revealed that the maximum stress of each modular member was within the maximum allowable stresses during lifting condition. However, the stress concentration at the connected area was more critical in comparison to the behavior of 40 m combined modular blocks during lifting time