• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.328-333
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• 2008

A series of pep tides binding to the HCV NS5B polymerase was selected from phage display peptide libraries. A conserved motif of Ser-Arg-X-Arg/Leu was identified among the selected peptides, and Pep2 (Trp-Ser-Arg-Pro-Arg-Ser-Leu) was chosen for further characterization. The binding of Pep2 to HCV NS5B in vivo was shown by a yeast two-hybrid assay and by subcellular colocalization analysis using immunofluorescence confocal microscopy. The in vitro interaction was also confirmed by GST pulldown assay. The replication of the HCV 1b subgenomic replicon was efficiently inhibited by the presence of the peptide. By using a subtractive biopanning against Pep2, the binding site of the peptide was mapped at the pocket of Pro388 to Pro391 in the thumb subdomain of the polymerase. A yeast two-hybrid analysis using Pro388Ala and Pro391Ala mutants of NS5B confirmed the binding.

• 공업화학
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• 제10권3호
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• pp.407-414
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• 1999

능금산법으로 제조된 페롭스카이트형 산화물에서 CO에 의한 NO의 환원반응에 대한 연구를 행하였다. 촉매는 주로 Lanthanoid계 페롭스카이트를 사용하였고, 활성을 증가시키기 위해 A, B site에 Sr, Ba 및 Fe, Mn 등을 치환시켰다. $LaCoO_3$ 촉매에서 A site에 Sr을 일부 치환시키면 NO전환율이 증가하였다. 한편 B site에 Fe나 Mn을 일부 치환시키면 NO의 전환율이 증가하였으나 Fe의 치환량이 커지면 오히려 전환율이 감소하였다. 한편 $La_{0.6}Sr_{0.4}Co_{0.8}Fe_{0.2}O_3 $ 촉매에 $SnO_2$나 $MnO_2$를 혼합하면 촉매활성이 증가하는 상승효과를 보였다. 반응물에 첨가된 물은 촉매활성을 감소시켰으나 촉매에 대한 물의 작용은 어느 정도 가역적이었다. 또한 반응물에 첨가된 이산화황은 NO의 전환율을 감소시켰다.

• 경영과정보연구
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• 제21권
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• pp.99-114
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• 2007

According as Internet based EC(Electronic Commerce) has been activated, More requirements for exchanging digital documents are being proposed in B2B(Business to Business) and B2G(Business to Government) environments. However, the documents can not be processed automatically, because the structures of documents in each site(companies, central/local governments) do not match. Therefore, W3C(World Wide Web Consortium) has proposed XML(eXtensible Markup Language) DTD(Document Type Declaration) technology to support structuring documents. The XML DTD technology can support interoperability between EC sites. And, XML DTD based documents can be processed automatically in every sites. They will improve efficiency of services in B2B and B2G environments. In this paper, we propose interoperable DTD architecture and IDEP(Interoperable DTD Exchange Protocol) protocol which supports searching DTDs and exchanging DTDs. This technology can popularize DTDs and digital documents.

• 한국산림과학회지
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• 제112권1호
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• pp.23-31
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• 2023

산림생산력의 예측은 지속가능한 산림경영이나 산림생태계서비스 증진을 위한 산림관리에 필수적인 것으로 알려져 있다. 본 연구는 전국 112개 신갈나무 임분을 대상으로 입지 및 토양 특성의 속성변수를 이용하여 지위 추정 모형을 개발하였다. 신갈나무 임분의 지위지수는 입지 및 토양 특성을 독립변수 한 일반최소제곱(Ordinary Least Squares, OLS) 및 부분최소제곱(Partial Least Squares, PLS) 회귀모형을 이용하여 유의적인 추정(P<0.05)이 가능하였다. 지위추정 회귀모형의 수정 결정계수(adjusted R²)는 입지 및 토양단면 속성변수의 회귀모형(A층: R²=0.29; B: R²=0.32)이, 토양 물리·화학적특성의 속성변수(A층: R²=0.09; B층: R²=0.21)보다 높게 나타났다. 한편, PLS 회귀모형(R²=0.20~0.32)은 OLS 회귀모형(R²=0.09~0.31)에 비해 지위지수 추정식의 설명력이 높았다. 본 연구로부터 신갈나무 임분의 입지 및 토양 특성을 이용한 지위 추정 회귀 모형이 개발되었으나, 결정계수 값이 낮아 회귀모형의 설명력을 향상시킬 수 있는 새로운 변수 개발이 필요할 것으로 사료되었다.

• 생명과학회지
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• 제14권3호
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• pp.459-466
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• 2004

영도 절영산과 단절된 산림지 3곳에 대한 식물종, 목본류의 다양성 양상을 조사하기 위해 18 plot(12개 50$\times$50m plot와 6곳 10$\times$100 m plots)를 설정하였다. 50${\times}$50m plots에 는 총 68종이 동정되고 목본류의 개체는 1562개였다. 최소 유의성 차이(LSD)에서 A지점(절영산)이 B지점(낮은 구릉지역)보다 높았다. Shannon-wiener의 다양성 지수에서 세 지역간 유의한 차이가 있었다(F=4.12, p＜0.05). 곰솔-갈참나무군락이 대부분 지역에서 우점하였다. 절영산과 태종대 지역에서 곰솔군락이 다른 방향보다 NE방향에 잘 분포하였다. 천선과 나무-곰솔군락은 C지역의 남쪽 plot에 잘 분포하였다. 영도 절영산과 해안 지역에 대한 수목의 공간적 분포는 이질적이며 패치(patchy)구조를 나타내었다.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 춘계학술발표대회
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• pp.29.2-29.2
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• 2011

B-site substitution with isovalent and donor impurities was compared in terms of the piezoelectric properties of Bi-based ABO3 perovskite ceramics. X-ray diffraction study revealed that both impurities bring about degradation in their ferroelectric properties as well as piezoelectric characteristics. However, there existed a difference between the isovalent and heterovalent impurities that influence a phase transformation of ferroelectric anisotropic-to-electrostrictive pseudocubic symmetry. Based upon analyses including the crystal, microstructure, dielectric, ferroelectric properties, we believed that A-site vacancies in the ABO3 ceramic significantly contribute to a ferroelectric-nonpolar phase transition, which give rise to lead to a giant strains. The present paper will discuss the origin of giant strains in Bi-based perovskite ceramics.

• Journal of Chest Surgery
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• 제23권4호
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• pp.707-714
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• 1990

Despite of mortality and morbidity rates that are higher than other forms of therapy, surgical resection has been mainstay for the treatment of esophageal cancer because of a prompt completeness as well as a high possibility of cure. But a substantial numbers of patients are unsuitable for surgical treatment and those undergoing resection have still poor long term survival rate. With hopes of improving long term survival, we have attempted multimodal approach, composed of preoperative induction chemotherapy utilizing Cisplatin and 5 \ulcornerFU, surgery and postoperative loco-regional radiotherapy for the treatment of esophageal cancer since 1985. During the period of 1967 \ulcorner1985, 27 patients[group A] were treated by surgery only and during recent 5 years 28 patients[group B] by multimodal treatment, Clinical review and comparison between the two groups were as follows: l. Applied surgical procedures were hand-sewn esophagogastrostomy, esophagocologastrostomy and esophagojejunostomy in group A. In group B, only esophagogastrostomy was underwent using stapler mainly. 2. Incidence of peri and postoperative complication showed no remarkable differences between the two groups, but the occurrences of leakage from the anastomotic site were 5[19% ] out of group A and 1[4%] out of group B. 3. The response rate to induction chemotherapy was 36% in group B, 4. The number of local cancer recurrence at the site of anastomosis was 6[22%] out of group A, whereas 2[7%] out of group B. 5. Postoperative I year and 2 year survival were 61%, 15% in group A and 75%, 42% in group B.

• The Korean Journal of Physiology and Pharmacology
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• 제18권2호
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• pp.155-161
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• 2014

Overexpression of amyloid precursor protein with the Swedish mutation causes abnormal hyperphosphorylation of the microtubule-associated protein tau. Hyperphosphorylated isoforms of tau are major components of neurofibrillary tangles, which are histopathological hallmarks of Alzheimer's disease. Protein phosphatase 2A (PP2A), a major tau protein phosphatase, consists of a structural A subunit, catalytic C subunit, and a variety of regulatory B subunits. The B subunits have been reported to modulate function of the PP2A holoenzyme by regulating substrate binding, enzyme activity, and subcellular localization. In the current study, we characterized regulatory B subunit-specific regulation of tau protein phosphorylation. We showed that the PP2A B subunit PPP2R2A mediated dephosphorylation of tau protein at Ser-199, Ser-202/Thr-205, Thr-231, Ser-262, and Ser-422. Down-regulation of PPP2R5D expression decreased tau phosphorylation at Ser-202/Thr-205, Thr-231, and Ser-422, which indicates activation of the tau kinase glycogen synthase kinase 3 beta ($GSK3{\beta}$) by PP2A with PPP2R5D subunit. The level of activating phosphorylation of the $GSK3{\beta}$ kinase Akt at Thr-308 and Ser-473 were both increased by PPP2R5D knockdown. We also characterized B subunit-specific phosphorylation sites in tau using mass spectrometric analysis. Liquid chromatography-mass spectrometry revealed that the phosphorylation status of the tau protein may be affected by PP2A, depending on the specific B subunits. These studies further our understanding of the function of various B subunits in mediating site-specific regulation of tau protein phosphorylation.

• 대한수의학회지
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• 제42권3호
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• pp.309-320
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• 2002

Animal bones that were excavated with earthenware at the Jongdal-ri shell mound archaeological site IV on Jeju Island were investigated morpholgically and osteometrically. This archaeological site is estimated to date from the late Tamra period to the unified Shilla Dynasty (A.D. 7C) based on the type of earthenware. The number of animal bones was 3,430 pieces(Quarter B : 758, Quarter C : 2,672). The animal species consisted of Cervus spp., Sus spp., Bos spp and Canivore. The animal bone pieces excavated at Quarter B consisted of those of Cervus(53.3%), Bos(2.8%) and Sus(13.7%). The bones of Cervus(53.3%), Bos(2.8%), Sus(13.7%) and Canivore(4.6%) were found at Quarter C. The greatest number of heads was unearthed as a whole, and the classification according to animal species showed that the heads were more visible than other bones. Forelimbs and hindlimbs were distributed evenly on the part of Cervus. The skeletons from Bos were identified in the order of heads, forelimbs and hindlimbs, while nearly all the Sus bones were found to be heads. These data suggests that Cervus, Sus and Bos are major fauna at the estimated period.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.139-146
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• 2000

7- Bromomethylbenz[a]anthracene is a known mutagen and carcinogen. The two major DNA adducts produced by this carcinogen, i.e., $N^2$-(benz[a]anthracen-7-yl methyl)-2'-deoxyguanosine (2, b[a]$a^2$G) and $N^6$-(benz[a]anthracen-7-ylmethyl)-2'-deoxyadenosine (4, b[a]$a^6$/A), as wel 1 as the simpler benzylated analogs,$N^2$-benzyl-2'deoxyguanosine (1, $bn^2$G) and $N^6$-benzyl-2'-deoxyadenosine (3, $bn^6$/A), were prepared by direct aralkylation of 2'-deoxyguanosine and 2'-deoxyadenosine. To determine the site-specific mutagenicity of these bulky exocyclic amino-substituted adducts, the suitably protected nucleosides were incorporated into 16-base oligodeoxyribonucleotides in place of a normal guanine or adenine residues which respectively are part of the ATG initiation codon for the lac Z' \alpha-complementation gene by using an in situ activation approach and automated phosphite triester synthetic methods. The base composition and the incorporation of the bulky adducts into synthetic oligonucleotides were characterized after purification of the modified oligonucleotides by enzymatic digestion and HPLC analysis.