• Title/Summary/Keyword: 20M10

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Monitoring the Manufacturing Characteristics of Aloe Gel-State Food (알로에 겔상 식품의 제조특성 모니터링)

  • 이기동;김숙경;권도영;박상렬
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.1
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    • pp.89-95
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    • 2003
  • Four-dimensional response surface methodology was used for monitoring the manufacturing characteristics of aloe gel-state food. The optimum conditions predicted for each corresponding sensory properties of aloe gel-state food were 87.38 mL (content of aloe juice), 0.16 g (content of konjac) and 0.19 g (content of carrageenan) in coloror of gel-state food, 83.84 mL, 0.17 g and 0.20 g in aroma of aloe gel-state food, 83.20 mL.0.15 g and 0.27 g taste of aloe gel-state food and 98.95 mL, 0.10 g and 0.23 g in texture of aloe gel-state food. Maximum chewiness of aloe gel-state food was in 113.05 mL aloe juice, 0.27 g konjac and 0.21 g carrageenan. The optimum conditions, which satisfied all sensory properties of gel-state food, were 88.23 mL, 0.15 g and 0.49 g in content of aloe juice, content of konjac and content of carrageenan, respectively.

Open Channel Block of Kv3.1 Currents by Genistein, a Tyrosine Kinase Inhibitor

  • Choi, Bok-Hee;Park, Ji-Hyun;Hahn, Sang-June
    • The Korean Journal of Physiology and Pharmacology
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    • v.10 no.2
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    • pp.71-77
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    • 2006
  • The goal of this study was to analyze the effects of genistein, a widely used tyrosine kinase inhibitor, on cloned Shaw-type $K^+$ currents, Kv3.1 which were stably expressed in Chinese hamster ovary (CHO) cells, using the whole-cell configuration of patch-clamp techniques. In whole-cell recordings, genistein at external concentrations from 10 to $100{\mu}M$ accelerated the rate of inactivation of Kv3.1 currents, thereby concentration-dependently reducing the current at the end of depolarizing pulse with an $IC_{50}$ value of $15.71{\pm}0.67{\mu}M$ and a Hill coefficient of $3.28{\pm}0.35$ (n=5). The time constant of activation at a 300 ms depolarizing test pulses from -80 mV to +40 mV was $1.01{\pm}0.04$ ms and $0.90{\pm}0.05$ ms (n=9) under control conditions and in the presence of $20{\mu}M$ genistein, respectively, indicating that the activation kinetics was not significantly modified by genistein. Genistein $(20{\mu}M)$ slowed the deactivation of the tail current elicited upon repolarization to -40 mV, thus inducing a crossover phenomenon. These results suggest that drug unbinding is required before Kv3.1 channels can close. Genistein-induced block was voltage-dependent, increasing in the voltage range $(-20\'mV{\sim}0\'mV)$ for channel opening, suggesting an open channel interaction. Genistein $(20{\mu}M)$ produced use-dependent block of Kv3.1 at a stimulation frequency of 1 Hz. The voltage dependence of steady-state inactivation of Kv3.1 was not changed by $20{\mu}M$ genistein. Our results indicate that genistein blocks directly Kv3.1 currents in concentration-, voltage-, time-dependent manners and the action of genistein on Kv3.1 is independent of tyrosine kinase inhibition.

Changes in the Quality and Physiological Activity of Angelica acutiloba Leaves in Various Packaging Materials during Storage (일당귀 잎의 포장방법이 저장 중 품질 및 생리활성에 미치는 영향)

  • Choi, Ji-Weon;Lee, Ji Hyun;Kim, Won Bae;Kim, Chang-Kug;Jung, Hyun Kyung;Hong, Yoon Pyo;Kim, Ji Gang
    • Korean Journal of Plant Resources
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    • v.30 no.1
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    • pp.29-37
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    • 2017
  • The effect of packaging materials on the quality and physiological activity of Angelica acutiloba leaves during storage at $4^{\circ}C$ for 20 days and after being transferred to room temperature for 2 days was studied. The experiment was conducted using three packaging materials: the corrugated box (control); corrugated box with $20{\mu}m$ HDPE perforated film liner; and corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner. Changes in weight, color, aroma, overall visual quality, antioxidant activity, and total flavonoids were investigated. Based on the result, the treatments using corrugated box with $20{\mu}m$ HDPE perforated film liner and the corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner, were effective in minimizing weight loss, and maintaining external color and overall quality as compared to corrugated box only (control). The corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner was more effective in maintaining antioxidant activity and total flavonoids than other treatments. Prolonging the freshness using corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner could increase the marketability of Angelica acutiloba leaves in the domestic market.

The Evaluation of UWB Wireless Communication Position Determination Function in an Enclosed Space for Fire Fighting (소방활동을 위한 밀폐공간 무선통신 측위성능 평가)

  • Kim, Dong-Hyun;Kim, Chung-Il
    • Fire Science and Engineering
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    • v.31 no.5
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    • pp.117-122
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    • 2017
  • The absence of fire fighters' connection to communication can bring about increased loss of life due to errors and delays in rescue and actions and danger to their own safety. Therefore, when communication infrastructure has been destroyed in an enclosed space, it is necessary to set up an emergency wire or wireless telecommunication environment to fire fighting and to secure safer environment to deal with the disaster by checking the location of fire fighters. In this research, for the establishment communication environment in an enclosed inside using Ultra-Wide Band (UWB) wireless communication network, the performance of communication position determination of a single UWB communication module was evaluated regarding 6 kinds of indoor surroundings. When the communication distance in an indoor environment of each 6 channels bandwidth of UWB communication frequency was measured, the results were as follows: the open space (Ave. 15.5 m, Max. 20 m), the corridor (Ave. 17.33 m, Max 20 m), inside retaining home furnishings (Ave. 15.3 m, Max. 20 m), vertical stairs (Ave. 4.33 m, Max. 6 m), horizontal space with a closed fire door (Ave. 6.5 m, Max. 17 m). It was also found out that the communication function and the distance function were best in the frequency range from 6489.6 to 1081.6 MHz by 7 Ch. Accordingly, the establishment of communication environment in an enclosed space is judged to be possible when UWB communication module is set up at 20 m apart and multiple channels are used.

A numerical study of the air fuel ratio effect on the combustion characteristics in a MILD combustor (공연비 변화가 MILD 연소 특성에 미치는 영향에 관한 해석적 연구)

  • Ha, Ji-Soo;Kim, Tae-Kwon;Shim, Sung-Hoon
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.6
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    • pp.587-592
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    • 2010
  • A numerical analysis of reactive flow in a MILD(Moderate and Intense Low oxygen Dilution) combustor is accomplished to elucidate the characteristics of combustion phenomena in the furnace with the variation of air fuel ratio. For the smaller magnitude of air injection velocity(10 m/s), the air flow could not penetrate toward upper part of furnace. On the other hand, the air flow suppresses the fuel flow for the case of air injection velocity 30 m/s. The air velocity 18 m/s is corresponding to the stoichiometric air flow velocity, and for that case, the air flows to relatively more upper part of the furnace when compared with the case of air injection velocity 10 m/s. The reaction zone is produced with the previous flow pattern, so that the reaction zone of the air injection velocity 10 m/s is biased to the air nozzle side and for the case of air injection velocity 30 m/s, the reaction zone is inclined to the fuel nozzle side. For the cases with the air injection velocities 16, 18, 20 m/s, the reaction zone is nearly located at the center between air nozzle and fuel nozzle. The maximum temperatures and NOx concentrations for the cases of air injection velocity 16, 18, 20 m/s are lower than the cases with air injection velocity 10, 30 m/s. From the present study, the stoichiometric air fuel ratio is considered as the most optimal operating condition for the NOx reduction.

The Development of Arginine-Selective Membrane Electrode Using Tissue Slices of the Rose of Sharon (무궁화꽃을 이용한 L-Arginine Sensor 開發에 關한 硏究)

  • Kim Ui-Rak;Nam Ki-jae;Choi Sung-Moon
    • Journal of the Korean Chemical Society
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    • v.36 no.5
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    • pp.753-759
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    • 1992
  • It is shown that receptacle tissue of the rose of sharon can serve as an effective biocatalyst by the construction of a potentiometric membrane electrode with response and selectivity for arginine. Electrodes using this tissue in conjunction with $NH_3$ gas sensing probes yield good response to arginine in $10^{-1}M to 10^{-3}M$ range and retain activity for a least 1 weeks. The arginine-selective membrane electrode using tissue slices of the rose of sharon have good selectivity in presence of other amino acids. The optimum conditions are pH 8.0, $25^{\circ}C$ and $20 {\mu}m$ thickness of slices in 0.1 M phosphate buffer solution and $K_m$ values is $6.4 {\times} 10^{-6}(M)$.

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Studies on the Survival Rate of Rapidly Frozen Bovine Immature Oocytes (소 미성숙 난포란의 급속동결 융해후 생존성에 관한 연구)

  • 김상근;이봉구
    • Journal of Embryo Transfer
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    • v.10 no.2
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    • pp.115-120
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    • 1995
  • This study was carried out to investigate On in vitro fertilization, survival rate and developmental rate of rapidly frozen bovine immature oocytes. Immature oocytes cultured for 1, 12, 24, 48 hours in 20% FCS + TCM-199 medium and thereafter rapidly freezing-thawed oocytes inseminated with capacitated sperm. The immature oocytes following dehydration by 1.5M DMSO + 2.0M glycerol + 0.25M sucrose + TCM 199 media + 20% FGS were directly plunged into liquid nitrogen and thawes in 3$0^{\circ}C$ water. Rapid freezing embryos co-cultured in 20% FCS + TCM-199 media containing hormones(21U/mL PMSG, 21U /mL hGG and 1 $\mu$g /mL 17$\beta$-estradiol) and cumulus cells(1 x 105-6 cells). Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro maturation and fertilization rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing4hawed were 57.1%, 45.7%, 37.1%, 25.7% and 40.0%, 31.4%, 20.0%, 11.4%, respectively. 2. The survival rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing-thawed were 33.3%, 26.7%, 20.0%, and 10.0%, respectively. The survival rate of rapid freezing4hawed immature oocytes was significantly lower than that of non-freezing oocytes. 3. The survival rate of rapid freezing4hawed excellent and good bovine embryos co-cultured in 20% FCS + TCM-199 media containing hormones(PMSG, hCG, 17$\beta$-estradiol) and cumulus cells 4 to 5 hrs and 20 to 24 hrs were 35.0%, 15.0% and 25.0%, 15.0% and 40.0%, 20.0% and 30.0%, 15.0%, respectively. The survival rate of embryos co-cultured in TCM-199 media containing hormones and cumulus cells was significantly higher than that of non co-culture.

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Distribution of Root System and Several Chemical Components of Soil on Low-Cutting Mulberry Field (낮추베기 뽕나무 뿌리와 몇몇 토양화학성 분포에 관한 조사연구)

  • 이원주
    • Journal of Sericultural and Entomological Science
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    • v.22 no.1
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    • pp.52-58
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    • 1980
  • Root system and distribution of soil components (pH, available P, K, Ca and Mg) were studied in a 20-year old, low-cutting mulberry field. The variety, kairyonezumigaeshi (Morus alba L.), was planted in a spacing of 1.8m${\times}$0.6m(740 tiess/10a). Roots and soil samples were taken from each of 196 blocks in a demension of 26${\times}$17${\times}$10cm(as shown in Fig. 1) from the soil between rows. 1. Whereas root system concentrated towards the stock, chemical components, pH, available P, Ca and Mg, were increasing toward the center of the row spacing. 2. Root system and distribution of chemical components between trees were not significantly different among blocks. 3. Roots were distributed most densely in a depth of 10∼20, intermediate 20∼30 and 30∼40, and least 0∼10cm. However, the concentrbtion of chemical components was the highest in a depth of 0∼10cm and decreased with soil depth. The soil. a depth of 0∼10cm on the center of row spacing, was the lowest in the root system and the richest in a chemical components. The results indicate that special fertilizinng management is required for the efficient absorption of nutrients.

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Effect of Plant Spacing on the Competitive Ability of Rice Growing in Association with Various Weed Communities at Different Nitrogen Levels (잡초군락형별로 본 질소시비량과 재식밀도가 수도의 경합력에 미치는 영향)

  • S. C, Kim;Keith, Moody
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.4
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    • pp.17-27
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    • 1980
  • All types of weed community became dominant by Monochoria vaginalis (Burm.) Presl. as the nitrogen level increased. The importance value (IV.) of M vaginalis rapidly increased with increasing nitrogen level whereas the LV. of other weed species decreased. At the 10 $\times$ 10cm plant spacing, M. vaginalis was almost totally suppressed at all nitrogen levels. At the 20 $\times$ 20cm plant spacing, the degree of suppression declined with increasing nitrogen level. At the 30 $\times$ 30cm plant spacing, there was no suppression of M vaginalis at the highest nitrogen level. The yield obtained at the 10 $\times$ 10cm plant spacing when M. vaginalis was present was not significantly different from that obtained from the weed free plot for all rice cultivars while it was significantly reduced by M vaginalis competition at the 20 $\times$ 20cm and 30 $\times$ 30cm plant spacings. The difference in yield caused by M vaginalis competition was primarily due to a reduction in the number of panicles at all nitrogen levels. There was a high negative correlation between grain yield and weed weight at heading. The yield reduction due to weed competition varied depending upon the nitrogen level.

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Effect of continuous pulsed electric fields treatments on quality of apple juice (사과주스의 품질에 미치는 pulsed electric field 연속 처리효과)

  • Ahn, Seong-Hwan;Lim, Jeong-Ho;Kim, Young-Ho;Chung, Suk Jin;Park, Kee-Jai
    • Food Science and Preservation
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    • v.20 no.5
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    • pp.650-658
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    • 2013
  • Apple juices were sterilized by continuous pulsed electric field (PEF) treatments of pulse width of 25 ${\mu}s$ at electric field intensity of 20.0 kV/cm, and with the varied pulse frequencies of 35 Hz (40 kJ/L), 55 Hz (70 kJ/L), 72 Hz (100 kJ/L) and 85 Hz (130 kJ/L). The PEF treatments of apple juice reduced the microbial counts from 5.3 log CFU/mL of initial state to 3.0 log CFU/mL after PEF treatment at energy density of 130 kJ/L. Also yeast and fungi after PEF treatments were reduced from 5.3 log CFU/mL to 3.0 log CFU/mL and Escherichia coli were from 5.3 log CFU/mL of initial state to 4.7 log CFU/mL to < $10^1$ CFU/mL. The soluble solids and free sugars did not significantly differ (p<0.05) depending on conditions of PEF treatment. The total phenolic contents and antioxidant activity such as the DPPH and ferric reducing antioxidant power (FRAP) by PEF treatments were significantly partly reduced, but the PEF-reduced value came in smaller quantities than the heat treatment at $65^{\circ}C$. The iterative PEF treatments with pulse width of 25 ${\mu}s$ and pulse frequency of 85 Hz at electric field intensity of 20.0 kV/cm showed limited in microbial reduction. Also, total phenolic contents and antioxidant activity such as DPPH and FRAP, significantly decreased depending on treatment numbers of PEF (p<0.05).