• Journal of Oral Medicine and Pain
• /
• v.30 no.4
• /
• pp.447-455
• /
• 2005

The purpose of this study was to examine the effect of Nd:YAG laser irradiation for sensory nerve conduction change. Nd:YAG laser was irradiated to mental region for 5 minutes with the parameters that 10 Hz and 20 Hz of 100 mJ/pulse. Target size of irradiation was 30 mm diameter of circle and target-tip distance was about 10 mm. Therefore, the power density were 212 $mW/cm^2$ and 424 $mW/cm^2$. Sensory nerve conduction was evaluated with $Neurometer^{\circled}$ CPT/C before and after Nd:YAG laser irradiation. As an outcomes, the current perception threshold(CPT) and pain tolerance threshold(PTT) were obtained. We made a comparison of these two values and the results were as follows. 1. There was no significant difference in CPT at 1W, 10 Hz and 2 W, 20 Hz Nd:YAG laser irradiation. 2. There was no significant difference in PTT at 10 Hz, 20 Hz of 100 mJ/pulse Nd:YAG laser. 3. There were no significant differences in CPT and PTT between 1 W, 10 Hz group and 2 W, 20 Hz group. Therefore, We can make a conclusion that Nd:YAG laser irradiation to mental region have no effect on mental nerve conduction in our study model.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.25 no.2
• /
• pp.70-74
• /
• 1989

This paper, described on the maneuverabilities of M.S. Cheju 402, the stern trawler (159 G.T.), training ship of Cheju National University which carried out the turning circle tests at helm angle 35$^{\circ}$ and 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$Ztests of her. The results obtained were as follows; 1. The advances of the starboard and port turning circles were 79.1m, about 2.6 times of the length of the ship, and 81.4m, about 2.7 times of it, respectively. 2. The rates of speed reduction were about 0.49 together, and mean values of turning angular velocity of her at helm angle 35$^{\circ}$ into the starboard and port sides were 4.3$^{\circ}$/sec and 4$^{\circ}$/sec during the turning movement. 3. Overshoot angles of starboard side werelarger than those of port side at all Z tests, and mean values of them of the starboard and port sides at 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$Z tests were 110.5$^{\circ}$ 20.5$^{\circ}$, 28.5$^{\circ}$ respectively. 4. The maneuvering indices K and T of experimental ship at 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$, Z tests were 0.755 and 3.468, 0.566, and 1.621, and 0.481 and 1.547 respectively. Consequently, the experimental ship showed that her turning ability was more in effective as the helm angle was becomed larger and her obeying ability was more effective as it was becomed larger.

• Biomolecules & Therapeutics
• /
• v.8 no.4
• /
• pp.338-348
• /
• 2000

The present study was attempted to determine the effect of 5'-(N'-ethylcarboxamido) adenosine (NECA), which is an potent $A_2$-adenosine receptor agonist, on catecholamine (CA) secretion evoked by cholinergic stimulation, membrane depolarization and calcium mobilization from the isolated perfused rat adrenal gland. NECA (20 nM) perfused into the adrenal vein for 60 min produced a time-related inhibition in CA secretion evoked by ACh (5.32x10$^{-3}$ M), high $K^{+}$(5.6x10$^{-2}$ M), DMPP (10$^{-4}$ M for 2 min), McN-A-343 (10$^{-4}$ M for 2 min), cyclopiazonic acid (10$^{-5}$ M for 4 min) and Bay-K-8644 (10$^{-5}$ M for 4 min). Also, in the presence of $\beta$,${\gamma}$-methylene adenosine-5'-triphosphate (MATP), which is also known to be a selective $P_{2x}$-purinergic receptor agonist, showed a similar inhibition elf CA release evoked by ACh, high potassium, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid. However, in adrenal glands preloaded with 20$\mu$M NECA for 20 min under the presence of 20$\mu$M 3-isobutyl-1-methyl-xanthine (IBMX), an adenosine receptors antagonist, CA secretory responses evoked by ACh, high potassium, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were much recovered in comparison to the case of NECA-treatment only. Taken together, these results indicate that NECA causes the marked inhibition of CA secretion evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors as well as by membrane depolarization. This inhibitory effect may be mediated by inhibiting influx of extracellular calcium and release in intracellular calcium in the rat adrenomedullary chromaffin cells through the adenosine receptor stimulation. Therefore, it is suggested that the inhibitory mechanism of adenosine receptor stimulation may play a modulatory role in regulating CA secretion.n.n.

• ALGAE
• /
• v.26 no.1
• /
• pp.79-86
• /
• 2011

Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and $25^{\circ}C$), at photon flux densities (10, 20, 40, and $80\;{\mu}mol\;m^{-2}s^{-1}$) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and $40\;{\mu}mol\;m^{-2}s^{-1}$ under 14 L : 10 D. Conchosporangial branches were produced under $10-80\;{\mu}mol\;m^{-2}s^{-1}$ at only $25^{\circ}C$, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of $10-20^{\circ}C$, 10 L : 14 D and $15-20^{\circ}C$, 14 L : 10 D. The foliose thalli grew very slowly at $5^{\circ}C$ and $30^{\circ}C$, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at $10-2^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at $10^{\circ}C$ under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.

• Microbiology and Biotechnology Letters
• /
• v.13 no.4
• /
• pp.391-395
• /
• 1985

In order to develope a protoplast fusion system for citric acid and SCP producing Candida lipolytica, the optimal conditions for the formation and regeneration of protoplast were examined and the protoplast fusion was performed. At the optimal conditions of growth phase and Zymolyase treatment, frequencies of protoplast formation were 98%. Approximately 20-30% of protoplasts were regenerated on the regeneration minimal medium containing 3% agar and 30mM $CaCl_2$ with the overlay of the same medium. The fusion frequencies, 4-5${\pm}$10$^{-4}$, were accomplished by the treatment of two nutritionally complementary auxotrophic protoplasts, L-14 ($lys^-$) and T-24 (X$30^-$), with 30% PEG 6000 containing 100mM $CaCl_2$ at $30^{\circ}C$ for 20 minutes.

• Journal of radiological science and technology
• /
• v.37 no.2
• /
• pp.135-141
• /
• 2014

This study was evaluated the linearity and reproducibility according to dose, and reproducibility according to delay time by changing tube current amount (5 mAs, 10 mAs, 16 mAs, 20 mAs, 25 mAs, 32 mAs respectively, which are low energy radiations) using Glass Dosimeter (GD) and piranha semiconductor dosimeter which are used for measuring exposure dose. Measurements of radiation dose were performed using external detector of piranha 657 which is multi-function QA device (RTI Electronic, Sweden). Conditions of measurement were 80 kVp, SSD 100 cm and exposure region is $10cm{\times}10cm$. Glass dosimeter was exposed to radiation. Twenty-four glass dosimeters were divided into six groups (5 mAs, 10 mAs, 16 mAs, 20 mAs, 25 mAs, 32 mAs respectively), then measured. This study was resulted by measuring the linearity and reproducibility according to change of tube current in low energy field. In dose characteristic of GD, this study could be useful as previous study with regard to dose characteristic according to change of tube voltage in low energy field.

• Journal of Plant Biology
• /
• v.32 no.4
• /
• pp.331-341
• /
• 1989

DNA methyltransferase was purified 282.6-fold from Chlamydomonas reinhardtii 21gr (mt+) gametic cell to examine the effect of polyamine on the enzyme acctivity. Polyacrylamide gel electrophoresis(PAGE) revealed at least three bands(1 major band, 2 minor bands). Among these, the major band represents DNA methyltransferase. Polyacrylamide gel electrophoresis in the presence of 0.1% sodium dodecylsulfate(SDS-PAGE) revealed a major band with M.W. 60,000. DNA methyltransferase activity was inhibited more effectively by spermine than by spermidine, and the inhibition by putrescine was smaller than spermine and spermidine. DNA methyltransferase activity was inhibited by 40% and 53% at 5mM and 20mM spermine, respectively. In the case of spermidine, the inhibition was 35% at 10mM and 44% at 20mM. However, the inhibition by putrescine appeared only above 5mM and reached about 25% at 20mM.

• Journal of the Korean institute of surface engineering
• /
• v.34 no.1
• /
• pp.39-48
• /
• 2001

Fe-Cr-Al alloy has been studied for application in automobile muffler materials due to good corrosion and oxidation resistance. In order to develop the automobile muffler materials, corrosion behaviors of electron beam physical vapor deposition (EB-PVD) coated surface of muffler matericls of muffler materials were investigated using potentiostat. For 0.1M NaCl solution, corrosion potential and pitting potential of Fe-20Cr-10Al was higher than that of Fe-5Cr- 10Al samples. Especially, in the case of Ti and Nb coated samples, pitting potential increased remarkably compared with non-coated samples. For 0.1M $CaCl_2$ solution, Ti-coated Fe-20Cr-10Al showed remarkably improved pitting corrosion resistance in comparison with non-coated Fe-20Cr-10Al and Fe-5Cr-10Al. The number and size of pits were decreased in the case of Ti coated samples in the 0.1M NaCl and 0.1M $CaCl_2$ solution.

• Korean journal of food and cookery science
• /
• v.23 no.1 s.97
• /
• pp.99-106
• /
• 2007

This study investigated the effect of UV-B irradiation on the quality of Pleurotus ostreatus. The changes of vitamin $D_2$ contents, color value and flavor pattern in mushrooms were analyzed by high-performance liquid chromatography (HPLC), chromameter and gas chromatography - surface acoustic wave (GC-SAW) electronic nose. By exposure to UV-B irradiation (0 kj/m$^2$, 10 kj/m$^2$, 20 kj/m$^2$), vitamin $D_2$ content increased from 0 (control) to 48.50 g/g (DM: dry matter, 10 kj/m$^2$) and 61.58 g/g (DM, 20 kj/m$^2$). Although there was no significant difference in L, a, b values among the three groups, flavor changes were detected by GC-SAW electronic nose. The number of peaks increased from 10 in the control group (0 kj/m$^2$), to 14 and 15 for the 10 kj/m$^2$ and 20 kj/m$^2$ groups, respectively. Nevertheless, the changes of flavor pattern were not detrimental to the mushroom quality. These results suggested that UV-B irradiation is an effective method to increase the vitamin $D_2$ content without degrading the quality.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.10a
• /
• pp.177-177
• /
• 2003

고품질 기능성 고추장을 개발하는데 고추장의 품질은 사용하는 재료, 배합비율, 제조방법, 숙성조건등에 따라 맛과 향기, 색 등에서 차이가 나타난다. 시장경쟁력에 맞추어 공장산 고추장에 기능성을 강화시키고 소비자의 기호성을 충족하기 위해 유기산과 비타민, 무기질이 풍부하고 다양한 생리적 기능성을 가진 국내산 매실엑기스를 공장산 고추장의 품질에 영향을 미치는 반제품에 농도별로 (0％(M0), 10%(M10), 20%(M20), 30%(M30)) 첨가하여 숙성 시 일어나는 화학적 성분변화를 조사하였다. 환원당은 9.6∼10.3%에서 11.9∼12.8%로 증가하였으며 매실엑기스의 첨가 농도가 낮을수록 높은 값을 나타내었다. 아미노산성질소는 숙성이 진행됨에 따라 증가하였으며 매실엑기스가 첨가된 시료의 경우 그 변화폭이 큰 것으로 나타났다. M0(대조구)의 pH는 5.45에서 발효 중 미생물에 의한 유기산생성으로 다소 감소하였으나 M10, M20, M30은 매실에 들어있는 유기산과 키토산으로 초기 pH가 2.87∼3.38로 낮아 변화 폭은 적은 것으로 나타났다. 적정산도는 숙성기간 중 증가하였으며, 대조구에 비해 메실엑기스의 첨가농도가 증가할수록 높은 값을 보였다. 염분(NaCl)은 같은 기간 중 감소하였으며 매실엑기스의 농도가 증가할수록 낮은 수치를 보였다.