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A Protocol of Ludox Treatment for Physiological and Molecular Biological Research of Freshwater Cyanobacteria (퇴적층 남조류 휴면세포의 생리적-분자생물학적 연구를 위한 Ludox 처리법)

  • Keonhee Kim;Kyeong-eun Yoo;Hye-in Ho;Chaehong Park;Hyunjin Kim;Soon-Jin Hwang
    • Korean Journal of Ecology and Environment
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    • v.56 no.1
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    • pp.94-103
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    • 2023
  • Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria's early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

Development and Testing of a RIVPACS-type Model to Assess the Ecosystem Health in Korean Streams: A Preliminary Study (저서성 대형무척추동물을 이용한 RIVPACS 유형의 하천생태계 건강성 평가법 국내 하천 적용성)

  • Da-Yeong Lee;Dae-Seong Lee;Joong-Hyuk Min;Young-Seuk Park
    • Korean Journal of Ecology and Environment
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    • v.56 no.1
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    • pp.45-56
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    • 2023
  • In stream ecosystem assessment, RIVPACS, which makes a simple but clear evaluation based on macroinvertebrate community, is widely used. In this study, a preliminary study was conducted to develop a RIVPACS-type model suitable for Korean streams nationwide. Reference streams were classified into two types(upstream and downstream), and a prediction model for macroinvertebrates was developed based on each family. A model for upstream was divided into 7 (train): 3 (test), and that for downstream was made using a leave-one-out method. Variables for the models were selected by non-metric multidimensional scaling, and seven variables were chosen, including elevation, slope, annual average temperature, stream width, forest ratio in land use, riffle ratio in hydrological characteristics, and boulder ratio in substrate composition. Stream order classified 3,224 sites as upstream and downstream, and community compositions of sites were predicted. The prediction was conducted for 30 macroinvertebrate families. Expected (E) and observed fauna (O) were compared using an ASPT biotic index, which is computed by dividing the BMWPK score into the number of families in a community. EQR values (i.e. O/E) for ASPT were used to assess stream condition. Lastly, we compared EQR to BMI, an index that is commonly used in the assessment. In the results, the average observed ASPT was 4.82 (±2.04 SD) and the expected one was 6.30 (±0.79 SD), and the expected ASPT was higher than the observed one. In the comparison between EQR and BMI index, EQR generally showed a higher value than the BMI index.

The Economic Impact of the Korean Port Industry on the National Economy : from the Viewpoint of Macroeconomics (한국항만산업이 국가경제에 미치는 영향에 관한 분석 - 거시경제의 관점에서 -)

  • Moon, S.H.
    • Journal of Korean Port Research
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    • v.6 no.2
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    • pp.65-92
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    • 1992
  • The Korean central government has not appreciate the full extent of the impact of seaports on the national economy. As a consequence port investment has not been given sufficient priority and capacity has failed to keep pace with demand. The principal reason for this failure is the fact that the linkages (or relationships) of the port transport industry with other sectors have not been quantified and fully appreciated. To overcome this dificiency this paper developed a port input-output model to determine the economic impact of the port industry on the national economy. This impact study was conducted by analysing the impact of the Korean port industry upon the national economy from the macroeconomic viewpoint, and identifying the spreading effects of port investments upon the nation's economy. The analysis of the economic impact of the port industry suggests that its contribution to the Korean economy is substantial. What the model shows is, in quantifiable terms, there are the strong economic linkages between the port industry and the other sectors of the national economy. The contribution of the port industry to the Korean economy was summarised in the Conclusion section.

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Development of Carbon Emission Factors and Biomass Allometric Equations for Metasequoia glyptostroboides and Platanus occidentalis in Urban Forests (정주지의 메타세쿼이아와 양버즘나무의 탄소 배출 계수 및 바이오매스 상대생장식 개발)

  • Jun-Young Jung;Subin Im;Hyun-Jun Kim;Kye-Han Lee
    • Journal of Korean Society of Forest Science
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    • v.112 no.2
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    • pp.127-135
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    • 2023
  • This study aimed to develop biomass allometric equations and estimate carbon emission factors, such as the wood density, biomass-expansion factor, and root-to-shoot ratio, for Platanus occidentalis and Metasequoia glyptostroboides planted in urban areas. Twenty M. glyptostroboides and 25 P. occidentalis trees were harvested, and the dry weights and stem volumes of stems, branches, leaves, and roots (>5 mm) were measured. The wood densities of M. glyptostroboides and P. occidentalis were 0.293 ± 0.008 g cm-3 and 0.509 ± 0.018 g cm-3, and the biomass-expansion factors were 1.738 ± 0.031 and 1.561 ± 0.035. The root-to-shoot ratios were 0.446 ± 0.009 and 0.402 ± 0.012. The uncertainty tests (coefficient of variation, %) gave 2.8% and 3.5% values for wood density, 1.8% and 2.3% for biomass-expansion factor, and 2.1% and 2.9% for root-to-shoot ratio, respectively. Among the developed allometric equations, Model I using the diameter at breast height (DBH) was suitable. The allometric equations of M. glyptostroboides and P. occidentalis above ground were y = 1.679 (DBH)1.315 and y = 0.505 (DBH)1.896, and the allometric equations of the root and total were y = 0.746 (DBH)1.315, y = 0.301 (DBH)1.751, y = 2.422 (DBH)1.316, and y = 0.787 (DBH)1.858. If the carbon-emission factors of this study and biomass allometric equations of the three developed models are used to estimate the carbon storage and biomass of urban forests, errors caused by not considering the use of fixed factors and the environmental differences can be reduced.

An Exploratory Study on Consumer Behavior of Digital Banking Deposits: Focusing on Bank Loyal Customers (디지털 뱅킹 정기예금의 소비자 행동 실태에 관한 탐색적 연구 -은행 충성고객을 중심으로-)

  • Inkwan Cho;Soo Kyung Park;Bong Gyou Lee
    • Journal of Service Research and Studies
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    • v.13 no.2
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    • pp.130-145
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    • 2023
  • The digital transformation of finance is accelerating, and digital banking has already become a major banking channel. Banks have traditionally placed importance on CRM(Customer Relationship Management) and have tried to retain their loyal customers, who contribute significantly to the bank, such as long-term transactions, holding accounts with a certain balance or more, and holding loans. In this situation, this study exploratorily analyzed the consumer behavior of digital banking deposits in a major bank of Korea(1,145 samples). Statistical analysis was performed using SPSS. The main findings of the study are summarized as follows. It was found that there were differences of consumer behavior in digital banking deposits by generation, and the MZ generation used digital banking more on holidays than other generations. As a result of analyzing the behavior of existing loyal customers and regular customers of digital banking deposit, there was a significant difference in both the amount and period of the deposit. It was confirmed that the existing loyal customers of the bank also engage in consumer behavior that contributes to the bank in digital banking. In addition, the interaction between the customer type and the date of sign up for the deposit period, which is the goal setting of financial consumers, it was found that there was a significant effect. This study empirically analyzed the consumer behavior of digital banking in a situation where decrease of bank branches and encounters with digital banking. The major concepts of the consumer behavior theory are Loyal Customer, Goal Pursuit, and Habit, which were confirmed in an example of digital banking. The results of this study can suggest practical implications for existing banks and Internet-only banks, including the importance of customer management in digital banking.

The Formation Mechanism and Distribution of Benthic Foraminiferal Assemblage in Continental Shelf of the northern East China Sea (북동중국해 대륙붕 저서성 유공충 군집 분포와 형성 기작)

  • Daun Jeong;Yeon Gyu Lee
    • Journal of Marine Life Science
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    • v.8 no.1
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    • pp.8-31
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    • 2023
  • To understand the distribution and formation mechanism of benthic foraminiferal assemblages, grain size analysis, 14C radiocarbon dating, and benthic foraminifera analysis were conducted on thirty-two surface sediments collected from the continental shelf of the northern East China Sea, respectively. Surface sediment was composed of sandy mud~muddy sand facies with an average of 52.04% of sand, 13.72% of silt, and 34.20% of clay. These sedimentary facies are palimpsest sediment. Benthic foraminifera was classified into a total of 48 genera and 104 species, including agglutinated foraminifera, calcareous-hyaline, and calcareous-porcelaneous foraminifera. The production rate of agglutinated foraminifera increased toward the Yangtze River area while that of planktonic foraminifera increased toward Jeju Island. Dominant species are Ammonia ketienziensis, Bolivina robusta, Eggella advena, Eilohedra nipponica, Pseudorotalia gamardii, Pseudoparrella naraensis. 14C radiocarbon datings of Bolivina robusta and Pseudorotalia gamardii with the highest production rate were 2,360±40 yr B.P. and 2,450±40 yr B.P., respectively. In the result of cluster analysis, three assemblages composed of P. gaimardii, B. robusta, and A. ketienziensis-P. naraensis were classified broadly. P. gaimardii assemblage is thought to be formed from about 2.5 yr B.P. at the sea area of the Yangtze River to 50 m in water depth affected by fresh water. B. robusta assemblage is thought to be formed from about 2.4 yr B.P. at the sea area of Jeju Island to 50~100 m affected by offshore water. And then, A. ketienziensisP. naraensis assemblage was formed in the northwest sea area (Central Yellow Sea Mud). These distributions and composition of benthic foraminiferal assemblages formed from about 2.5 yr B.P. in the northern East China Sea are thought to be due to the change of benthic ecology environment that occurred by the sea level increase during the late Holocene.

A Study on the Garden Culture and Ideology based on the Confucianism and Taoism of the Song Dynasty - Focused on Zhū Xī(朱熹) and Báiyùchán(白玉蟾) - (송대(宋代) 유가와 도교에 근거한 원림 문화와 사상 고찰 - 주희(朱熹)와 백옥섬(白玉蟾)을 중심으로 -)

  • Park So-Hyun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.41 no.1
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    • pp.10-20
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    • 2023
  • Zhū Xī, the representative of Confucianism, and Báiyùchán, the representative of Taoism in the South Song Dynasty, showed different sense of appreciation and enjoyment on the same space that was Mountain Wǔyí in their ideologically cultural ways. Based on the temples Wŭyíjīngshè(武夷精舍) where Zhū Xī stayed and Zhĭzhĭān(止止庵) where Báiyùchán resided, this study revealed their lives in such temples to look into their appreciation on ideology and space. Then, based on the words 'YiBoEumYeong [移步吟詠]' shown on the poetry they chanted in relation with Wǔyíjiǔqū from its 1st valley to its 9th valley, this study examines their understanding of scenery and system of appreciation that appeared in dynamic ways to conclude: First, even same scenery shows different understanding of scenery and appreciation of space in accordance with the viewers' thinking ways of culture. Second, as the Confucianism and Taoism influenced in ideologically cultural ways to develop each other in the Song dynasty, they absorbed their merits each other to supplement shortcomings in their own. In this process, they made it clear that their own propositions were different between them in their essential meanings although they used common terms for such propositions. Third, as the Confucian master who compiled the Neo-Confucianism of the South Song dynasty, Zhū Xī regarded Wŭyíjīngshè and Wǔyíjiǔqū as a place of learning and a place of seeking the truth to go for 'being unified with nature' so that everyday life can be united with Tao of Li [理] everywhere beyond the limited appreciation of the scenery. That is, this thought works for 'recovery of nature of our own [復其性]', the learning goal of Confucianism, and is aimed to 'cultivate the essential nature of our own(性情涵養)' through such beautiful nature. Fourth, as the master of Keumdan family of the South Song Taoism, Báiyùchán regarded Zhĭzhĭān and Wǔyíjiǔqū as a Taoist temple that has a long history rooting from Taesangwon temple, a clean place of discipline to become a Taoist hermit through hard training. He, therefore, directly referred to Zhĭzhĭān and Wǔyíjiǔqū in relation with the Taoist legends remaining in Wǔyíjiǔqū such as hermits' dinners, female hermits, leaving the human world as a hermit and so on as ways for becoming a hermit so that he went for the level of perfectly going out of human world and becoming a hermit. He, therefore, defined Mountain Wǔyí as a world and universe of hermits where he himself too hovered between outside and inside of poetry literature as a hermit through the mood and attitude of keeping himself enjoying the scenery as a hermit.

Temporal variation in the community structure of green tide forming macroalgae(Chlorophyta; genus Ulva) on the coast of Jeju Island, Korea based on DNA barcoding (DNA 바코드를 이용한 제주도 연안 파래대발생(green tide)을 형성하는 갈파래(genus Ulva) 군집구조 및 주요 종 구성의 시간적 변이)

  • Hye Jin Park;Seo Yeon Byeon;Sang Rul Park;Hyuk Je Lee
    • Korean Journal of Environmental Biology
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    • v.40 no.4
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    • pp.464-476
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    • 2022
  • In recent years, macroalgal bloom occurs frequently in coastal oceans worldwide. It might be attributed to accelerating climate change. "Green tide" events caused by proliferation of green macroalgae (Ulva spp.) not only damage the local economy, but also harm coastal environments. These nuisance events have become common across several coastal regions of continents. In Korea, green tide incidences are readily seen throughout the year along the coastlines of Jeju Island, particularly the northeastern coast, since the 2000s. Ulva species are notorious to be difficult for morphology-based species identification due to their high degrees of phenotypic plasticity. In this study, to investigate temporal variation in Ulva community structure on Jeju Island between 2015 and 2020, chloroplast barcode tufA gene was sequenced and phylogenetically analyzed for 152 specimens from 24 sites. We found that Ulva ohnoi and Ulva pertusa known to be originated from subtropical regions were the most predominant all year round, suggesting that these two species contributed the most to local green tides in this region. While U. pertusa was relatively stable in frequency during 2015 to 2020, U. ohnoi increased 16% in frequency in 2020 (36.84%), which might be associated with rising sea surface temperature from which U. ohnoi could benefit. Two species (Ulva flexuosa, Ulva procera) of origins of Europe should be continuously monitored. The findings of this study provide valuable information and molecular genetic data of genus Ulva occurring in southern coasts of Korea, which will help mitigate negative influences of green tide events on Korea coast.

Effect of temperature on oviposition of Spodoptera frugiperda (Lepidoptera: Noctuidae) and ovipositional characteristics in corn fields (온도가 열대거세미나방 산란에 미치는 영향 및 옥수수 포장에서의 산란 특성)

  • Hyung Cheol Moon;Min Kyung Choi;Su Ji Jang;Jang Ho Lee;Ju Hee Kim;Hyong Gwon Chon
    • Korean Journal of Environmental Biology
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    • v.40 no.3
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    • pp.281-289
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    • 2022
  • The effect of five different constant temperatures (18, 21, 24, 27, and 30±1℃) and a photoperiod of 14 : 10 (L :D) h on the reproduction parameters of Spodoptera frugiperda was studied. The longevity of adult female S. frugiperda decreased with increasing temperature (22.4 days at 21℃ and 13.9 days at 30℃) but not at 18℃. The pre-oviposition period and oviposition period was the shortest at 30℃ compared to the other temperatures. The total fecundity egg count was 887.4, 1,246.4, 1,348.9, 1,154.9, and 1,034.2 at 18, 21, 24, 27 and 30℃, respectively, during its life span. The survival rate of female S. frugiperda decreased rapidly after 13 days at 18℃, after 14 days at 21℃, after 15 days at 27℃, and after 9 days at 24℃, and 30℃. On the third day after the start of oviposition, 50% of the total fecundity was accomplished. In corn fields at less than the 10-leaf stage, the distribution of S. frugiperda egg masses was observed in the middle and lower plant regions, corresponding to 46.8% and 41.4% of the total egg masses, respectively. Egg masses were mostly found on the underside of the leaf blade (abaxial) of corn(66.7%). After releasing S. frugiperda adults on May 12, May 17, May 25, and May 30, the number of eggs per egg mass was 89.9, 88.5, 126.6, and 127.9, respectively. Egg masses of the subsequent generations of S. frugiperda were observed from late June, and the number of eggs per egg mass was 155.8 in late June, 270.7 in early July, and 303.5 in mid-July.

Effect of Planting Date and Hybrid on the Agronomic Characteristics, Forage Production and Feed Value of Corn for Silage (파종시기 및 품종이 사일리지용 옥수수의 생육특성, 사초생산성 및 사료가치에 미치는 영향)

  • Bae, Myeong Jin;Chung, Sung Heon;Kim, Jong Duk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.1
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    • pp.54-60
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    • 2022
  • The planting date of corn for silage has been delayed because of spring drought and double cropping system in Korea. This experiment was conducted to evaluate agronomic characteristics, forage production and feed value of corn at April and May in 2019. Experimental design was a split-plot with three replications. Planting dates (12 April and 10 May) were designated to the main plot, and corn hybrids ('P0928', 'P1543' and 'P2088') to the subplot. The silking days of the early planting date (12 April) was 79 days and that of the late planting date (10 May) was 66 days (p<0.0001), however, there were no significant differences among the corn hybrids. Ear height of the late planting date was higher than that of the early planting (p<0.05), while there were no significant differences in plant height of corn. Insect resistance at the early planting was lower than that of late planting (p<0.05), however, lodging resistance was no significant difference at planting date. The rice black streaked virus (RBSDV) infection of early planting was 3.7% and that of late planting was 0.3% (p<0.001). Dry matter (DM) contents of stover, ear and whole plant had significant difference at planting date (p<0.05). And differences in ear percentages were observed among the corn hybrids (p<0.01). And ear percentages of early maturing corn ('P0928') was higher than for other hybrids. Ear percentage at the early planting date was higher than that at the late planting date (p<0.01). DM and total digestible nutrients (TDN) yields had significant difference at planting date, however, there were no significant differences among the corn hybrids. DM and TDN yields at the late planting (21,678 kg/ha and 14,878 kg/ha) were higher than those of the early planting (13,732 kg/ha and 9,830 kg/ha). Crude protein content at the early planting date was higher than that of the late planting. Acid detergent fiber content of the late planting was lower than that of the early planting date (p<0.01), while there were no significant neutral detergent fiber content difference among the corn tested. Calculated net energy for lactation (NEL) and TDN at the early planting were higher than those of at the late planting (p<0.01). Results of this our study indicate that the late planting date (May) is better than early planting date (April) in forage yield and feed value of corn. Therefore, the delay of planting date by May was more suitable for use in cropping system.