• Journal of the Korean Wood Science and Technology
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• v.42 no.6
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• pp.758-767
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• 2014

In this study, we selected an Escherichia coli strain (E. coli MG 1655) metabolizing arabinose derived from acid hydrolyzed black liquor as a carbon source and investigated effect of organic acids (acetic acid, formic acid, and lactic acid) presented in black liquor on growth of the E. coli MG 1655. We measured growth of E. coli MG 1655 under various concentration of each and combined three kinds of organic acids. The E. coli MG 1655 shows tolerance to acetic acid, lactic acid and formic acid at these concentrations ($1.0g/{\ell}$ acetic acid, $1.2g/{\ell}$ lactic acid and $0.8g/{\ell}$ formic acid, respectively), but displays some growth retardation over $1.5g/{\ell}$ acetic acid, lactic acid $2.0g/{\ell}$, and formic acid $1.2g/{\ell}$, respectively. In addition, formic acid was shown to be a critical factor affecting growth of the E. coli MG 1655 in the presence of three kinds of organic acids. These results indicate that the inhibitors should be removed at least $1.0g/{\ell}$ of acetic acid, $1.2g/{\ell}$ of lactic acid, $0.8g/{\ell}$ of formic acid for normal cell growth required for high yield fermentation. In addition, there is a need to construct recombinant strains that may be resistant to the same or higher organic acids concentration (> $1.2g/{\ell}$) in the growth.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.9
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• pp.1282-1289
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• 2001

One feeding trial and two metabolic trials were conducted to investigate the effects of lysine to protein ratio in practical swine diets on growth performance and efficiency of nitrogen retention and utilization in different growing phases. In Trial one (the feeding trial), 90 mixed sex pigs weighing $9.1{\pm}1.4kg$ (Duroc ${\times}$ Landrance ${\times}$ Beijing Black) were used to study the effects of concentrations of 5.2, 5.3, 5.8, 6.4 and 7.2 g lysine/100 g CP in diets containing 1.2% lysine on growth performance and serum urea nitrogen. The results showed that feed conversion efficiency and economic efficiency were best for pigs fed the diet containing the lysine concentration of 5.8 g /100 g crude protein. Serum urea nitrogen concentration decreased linearly (p=0.0009) and serum free lysine content increased linearly (p=0.0017) as the lysine to protein ratio in diets increased from 5.2 to 7.2 g/100 g. In Trials two and three (the metabolic trials), five growing barrows (Duroc ${\times}$ Landrance ${\times}$ Beijing black), with initial body weights of approximately $26{\pm}2.4kg$ and $56.3{\pm}3.5kg$, respectively, were allotted to five dietary treatments according to a $5{\times}5$ Latin square design. Trial two contained 5.2, 5.7, 6.1, 6.7 and 6.8 g lysine/100 g CP treatments. Trial three contained 4.6, 5.0, 5.6, 6.1 and 6.6 g lysine/100 g CP treatments. The results showed that nitrogen retention in growing pigs decreased linearly (p=0.0011 in Trial two; p=0.0099 in Trial three) as the lysine to protein ratio in diets increased. The ratio of lysine to protein in diets resulting in maximum nitrogen retention was 5.2 g/100 g and 5.0 g/100 g in Trial two and Trial three, respectively. In Trial two, apparent biological value and gross nitrogen efficiency increased linearly (p=0.0135 and p=0.0192, respectively) as the lysine to protein ratio increased from 5.2 to 6.8 g lysine/100 g CP. In summary, we concluded that the optimal Lysine to Protein Ratios for 8-20 kg and 20-80 kg pigs were 5.8 g/100 g and 5.0 to 5.2 g/100 g, respectively.

• Journal of Naturopathy
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• v.7 no.2
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• pp.63-69
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• 2018

Purpose: The purpose of this study was to investigate the effects of Bacillus fermentation broth (ENM) on the human NK cell activity, and bone matrix density, matrix content and area of hamsters fed ENM. Methods: NK cell activity was tested, and bone mineral density were measured by x-ray. Results: NK cell activity was significantly higher in the control group (644.71 pg/ml) and in the test group (1796.37 pg/ml) (p<.004). Test groups were significantly increased by 1110.37 pg/ml in the pre-test and 1796.37 pg/ml in the post-test(p<.001). Behavioral observations after feeding ENM to hamsters showed normal behavior with no difference between control and test groups. The hamster body weight of the control was 106 g at the initial and final 27 days. In the test group, the initial weight was 96.6 g and the final 27 days was 114 g. No morphological changes were observed in the X-ray photographs of the hamster hind legs. The bone matrix density was 0.059 g/cm³ in control and 0.062 g/cm³ in the test, which was increased by 0.003 g/cm³. The bone matrix content was 0.175 g in the control and 0.196 g in the test. The bone area was 2.95 cm² in the control and 3.19 cm² in the test, which was increased by 0.19 cm². In the autopsy, neither the control nor the test group showed any remarkable abnormality, and each organ was normal. Conclusions: It is thought that ingestion of ENM is useful for immunity enhancement.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.5
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• pp.233-240
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• 1989

The concentration of mecury was measured in the seawater, sediment, Mytilus edulis and suspended solid in the coastal area of Onsan. Samples were collected at spring and neap tide in the period from October 9 to November 2, 1984. The range and mean of mecury concentration in the seawater are $0.08\~0.73{\mu}g/\iota\;and\;0.19{\mu}g/\iota$. The range and mean of mecury concentration in the suspended solid was $0.70\~17.0{\mu}g/g$ TSS and $4.6{\mu}g/g$ TSS respectively. The average concentration of mecury in Mytilus edulis was 0.10mg/kg which was higher than that of the reference area, Chung Mu. The mean value of concentration factor of mercury in wet base is 500 in Mytilus edulis. The average concentration of mecury in sediment was 0.15mg/kg.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.9 no.2
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• pp.63-71
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• 2011

A large volume of uranium electrokinetic leachate has been generated during the electrokinetic decontamination to remove uranium from contaminated soil. The treatment technology for the reuse of the uranium leachate was developed. The concentration of uranium in the generated uranium leachate was 180 ppm and concentrations of Mg(II), K(I), Fe(II), and Al(III) ions ranged from 20 ppm to 1,210 ppm. The treatment process for uranium leachate consisted mainly of mixing and cohesion, precipitation, concentration, and filtration. In order to obtain the pH=11 of a precipitate solution, the calcium hydroxide needs to be 3.0g/100ml and the sodium hydroxide needed to be 2.7g/100ml. The results of several precipitation experiments showed that a mixture of NaOH+0.2g alum+0.15g magnetite was an optimal precipitant for filtration. The average particle size of precipitate with NaOH+alum+0.15g magnetite was $600\;{\mu}m$. Because the total value of metal concentrations in supernatant at pH=9 was the smallest, sodium hydroxide should be added with 0.2g alum and 0.15g magnetite for pH=9 of leachate.

• KSBB Journal
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• v.13 no.5
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• pp.615-620
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• 1998

The readily fermentable carbon sources in swine were acetic acid, propionic acid and butyric acid at the average concentrations of 7.2 g/L, 2.2 g/L and 2.7 g/L, respectively. The swine waste also contained excess nitrogen and other mineral sources. In shake flask experiments, the optimal range of cell growth for Azotobacter vinelandii UWD were 1.0∼3.5 g/L of acetic acid, 0.7∼2.0 g/L of propionic acid and 0.5∼2.0 g/L of butyric acid. A mixture of these three acids simulating two times diluted swine waste supported the best cell growth but the amount of carbon sources was limited. In shake flask and fermentor experiments, an addition of 30 g/L of glucose increased the final cell dry weight 8 times while the final poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) concentration increased 86 times compared with using acid mixture only. A. vinelandii UWD preferred organic acids in the sequence of acetic acid, propionic acid, butyric acid, and valeric acid.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.5
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• pp.1-12
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• 2009

This research was initiated to investigate the revegetation characteristics under the seeding amount treatments of cool-season turfgrasses on the disturbed slope. 4 different seeding amounts of cool-season turfgrasses (0.0 $g/m^2$, 1.5 $g/m^2$, 3.0 $g/m^2$, 6.0 $g/m^2$) with the same seeding amounts of native woody (Rhus chinensis, Albizzia julibrissin) and herbaceous plants (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena) were treated with 3 replications on the experimental disturbed slope. Data such as soil hardness (mm), moisture content (%), surface coverage rate (%), the number of each germinating plant and plant height were analyzed. There were no statistic differences observed in the soil hardness and the soil moisture content while a significant difference was observed in the surface coverage rates. The surface coverage rates were higher in 3.0 $g/m^2$ and 6.0 $g/m^2$ plots compared to the rest two plots of cool-season turfgrasses. However, the emergence of native woody and herbaceous plants was relatively low in 3.0 $g/m^2$ and 6.0 $g/m^2$ plots of high seeding amounts of cool-season turfgrasses. This result indicated that high seeding amounts of cool-season turfgrasses could decrease the emergence of native woody and herbaceous plants. We concluded that 1.5 g~3.0 $g/m^2$ seeding amounts of cool-season turfgrasses was optimum level for the balanced emergence of cool-season turfgrasses, native woody and herbaceous plants in the viewpoint of the ecological disturbed slope revegetation.

• Korean journal of food and cookery science
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• v.15 no.2
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• pp.197-202
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• 1999

The objective of this study was to standardized the cooking method of Imjasootang, a traditional health food in summer. By mixing chicken soup with 20 g (I$_1$), 30 g (I$_2$), 40 g (I$_3$), 50 g (I$_4$) of seasam seeds, the distinctive characteristic and level of preference of Imjasootang were examined through the analyses of the proximate composition, fatty acid contents, color index, turbidity, brix$^{\circ}$, and sensory evaluation. 1. The proximate composition of Imjasootang was 90.2∼95.1% of total moisture, 0.985∼1.271% of crude protein, 0.90∼0.98% of crude fat, 1.592∼2.456% of nonfiber, 0.003∼0.004% of fiber, and 0.77∼0.97% of crude ash. Fatty acid were composed of 15.15∼17.36% of saturated fatty acid and 79.73∼82.54% of unsaturated fatty acid. Imjasootang contains high level of oleic acid and linoleic acid which were dependant on the content of seasame seeds. 2. For color index of samples, the lightness ranged 47.2∼56.9, “a”values 1.82∼2.15%, and “b”values 15.5∼16.5%. The turbidity of samples ranged 39∼61%, in which I$_4$ (chicken stock 100 g+sesame seed 50 g+water 25 g) was the most turbid. Solid content was also the highest in I$_4$, ranging 1.4∼5.1%. 3. In sensory evaluation, all sesame seed Imjasootangs had no significance differences in appearance. The higher the level of sesame seed in Imjasootangs, the more Imjasootang was preferred in terms of nutty aroma, color nutty taste. and viscosity. I$_4$ had the strongest nutty aroma. I$_2$ (chicken stock 100 g+sesame seed 30 g+water 15 g) and I$_3$ (chicken stock 100 g+sesame seed 40 g+water 20 g) were the best in the overall preference.

• Journal of Korean Neurosurgical Society
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• v.61 no.6
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• pp.669-679
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• 2018

Objective : To compare the spinal bone fusion properties of activin A/BMP2 chimera (AB204) with recombinant human bone morphogenetic protein (rhBMP2) using a rat posterolateral spinal fusion model. Methods : The study was designed to compare the effects and property at different dosages of AB204 and rhBMP2 on spinal bone fusion. Sixty-one male Sprague-Dawley rats underwent posterolateral lumbar spinal fusion using one of nine treatments during the study, that is, sham; osteon only; $3.0{\mu}g$, $6.0{\mu}g$, or $10.0{\mu}g$ of rhBMP2 with osteon; and $1.0{\mu}g$, $3.0{\mu}g$, $6.0{\mu}g$, or $10.0{\mu}g$ of AB204 with osteon. The effects and property on spinal bone fusion was calculated at 4 and 8 weeks after treatment using the scores of physical palpation, simple radiograph, micro-computed tomography, and immunohistochemistry. Results : Bone fusion scores were significantly higher for $10.0{\mu}g$ AB204 and $10.0{\mu}g$ rhBMP2 than for osteon only or $1.0{\mu}g$ AB204. AB204 exhibited more prolonged osteoblastic activity than rhBMP2. Bone fusion properties of AB204 were similar with the properties of rhBMP2 at doses of 6.0 and $10.0{\mu}g$, but, the properties of AB204 at doses of $3.0{\mu}g$ exhibited better than the properties of rhBMP2 at doses of $3.0{\mu}g$. Conclusion : AB204 chimeras could to be more potent for treating spinal bone fusion than rhBMP2 substitutes with increased osteoblastic activity for over a longer period.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.8
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• pp.1098-1106
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• 2004

In a split-unit design, 12 Javan rusa (Cervus timorensis russa) stags (6 castrated and 6 entire) were used to investigate seasonal (winter, spring and summer) effects on intake, digestibility of food constituents, volatile fatty acid profile, and nitrogen retention, when given lucerne (Medicago sativa) or rhodes grass (Chloris guyana) hays. Entire stags ate 9% more dry matter (DM) than castrates (p<0.05). Both castrated and entire stags exhibited seasonal changes in DM intake, these being higher (p<0.05) in winter (62.3 g/kg $W^{0.75}$) than spring (56.9 g/kg $W^{0.75}$) or summer (55.3 g/kg $W^{0.75}$). Intakes of lucerne hay (211 g protein/kg DM) were significantly higher than of rhodes grass hay (49 g protein/kg DM) in all seasons (p<0.05). Digestibilities of DM (0.58), neutral detergent fibre (0.59) and acid detergent fibre (0.47) were similar between castrates and entires. DM digestibility was higher (p<0.0001) for lucerne (0.66) than rhodes grass (0.55), and was higher (p<0.05) in winter (0.60) than spring (0.58) or summer (0.57). The ruminal acetate concentrations were higher (p<0.001) in spring than summer (78 and 73 molar % respectively). Apparent digestibility of protein was significantly less (p<0.0001) for rhodes grass (0.37) than lucerne (0.75). N retention was positive for lucerne (15.2 g/d) but negative for rhodes grass (-2.8 g/d) (p<0.0001), and was higher (p<0.001) in summer (12.0 g/d) than spring (4.3 g/d) or winter (2.4 g/d). The tropical rusa deer exhibits seasonal variations in feed intake, food constituent digestibilities, VFA profile and N retention. Castration did not alter these traits. The results do not support the view that rusa deer can thrive on low-quality pastures. The productivity and commercial exploitation of rusa deer could be optimised if they are given high-protein feed during spring and summer.