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Effect of oral administration of egg yolk on oxidation and esterification of hepatic fatty acid in rats (랫드에서 난황의 경구투여가 간 지방산의 산화, 에스터화에 미치는 영향)

  • Kim, Chang-Hyun;Um, Kyung-Hwan;Shin, Jong-Suh
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.3
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    • pp.398-408
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    • 2020
  • The purpose of the present study was to investigate the mechanism of metabolic partitioning between oxidization and esterification of liver fatty acids synthesized and secreted by the liver from egg yolk-fed laboratory rats. Animals were divided into four groups and orally administered egg yolk daily for 30 days: CON (control group, 1.0 g of saline solution), T1 (1.0 g of pork belly oil), T2 (1.0 g of egg yolk), and T3 (1.0 g of pork belly oil and 1.0 g of egg yolk, alternatively each week). The accumulation rate of [14C]-labelled lipid in liver was lowest in T2 among all treatments (P<0.05). Phospholipid secretion was higher in T2 than other treatments (P<0.05). The triglycerol secretion was highest in T1 and higher in the order of CON, T3, and T2 (P<0.05). Metabolic partitioning rate of phospholipid from total glycerolipid was highest in T2, followed by T3, CON and T1 (P<0.05). The 14CO2 production from total glycerolipid was the highest in T2 and showed a high oxidation rate compared to CON, T1, and T3 (P<0.05). Metabolic partitioning of glycerolipid from the liver decreased in triglycerol of T2 compared to CON, T1, and T3, whereas phospholipids of T2 increased (P<0.05).

실관 생물반응기대 고정화된 Enterococcus faecalis RKY1에 의한 숙신산 생산특성

  • 위영중;윤종선;류화원
    • KSBB Journal
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    • v.17 no.2
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    • pp.182-188
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    • 2002
  • Enterococcus faecalis RKY1 cells were immobilized in an asymmetric hollow fiber bioreactor for application to the continuous production of succinic acid. The media was fed into shell-side of the module using a peristaltic pump, and the products were collected through lumen-side outlet. The number of hollow fibers within the module did not affect the bioreactor efficiency in the transverse operated hollow fiber bioreactor. The steady state at the outlet of hollow fiber bioreactor was reached after 24 hr cultivation at flow rate of 0.25 mL/mim, 12 hr at 0.5 L/min, and 9 hr at 1.0 mL/mm, respectively. The succinate and fumarate concentrations within the hollow fiber bioreactor, however, were as changeful as increasing the flow rate. During continuous operation with the flow rates between 0.5 and 2.0 mL, the productivity of succinate was 8.0-10.9 g/L $.$ hr at 30 g/L fumarate, 4.9-14.9 g/L hr at 50 g/L fumarate, and 7.2-17.1 g/L hr at 80 galL fumarate, respectively.

Nutritional Value of Yacon as a Food (야콘의 식품 영양적 가치)

  • Kim, Su-Jeong;Jin, Yong-Ik;Nam, Jung-Hwan;Hong, Soo-Young;Chang, Dong-Chil;Jeong, Jin-Cheol
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2012.05a
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    • pp.17-17
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    • 2012
  • 야콘의 유전자원 4계통(HLY1, HLY2, HLY3, HLY4)에 대하여 단백질, 회분, 탄수화물, 식이섬유, 비타민, 프락토올리고당 등의 영양 성분을 분석하였다. 2010년 강원도 평창군 진부면(해발고도 560 m) 시험 포장에서 5월 12일에 정식하여 10월 20일에 수확한 야콘을 시료로 사용하여 농촌진흥청 식품 분석표에 준하여 분석하였다. 야콘은 신선한 괴근 100g당 열량 44~59kcal, 수분함량이 86~87%, 회분 0.2~0.4g, 단백질 0.5~0.7g, 탄수화물 11.0~13.1g, 지방 0.1~0.2g, 식이섬유 1.4~1.7g, 칼슘 8~10mg, 인 24~31mg, 철 0~0.1mg, 나트륨 1~2mg, 칼륨 154~208mg, 레티놀 0.001~0.004mg, 카로틴 0.001~0.024mg, 티아민 0.03~0.11mg, 리보플라빈 0.02~0.03mg, 니아신 0.3~0.4mg, 아스코르브산 10.6~29.6mg이 들어 있는 것으로 조사되었다. 또한 야콘의 괴근에는 당 함량이 많았는데 생체중 100g당 프락토올리고당 5.7~10.1g, 글루코스 0.3~0.9g, 프럭토스 0.2~0.7g, 슈크로스 0.4~3.3g이 함유되어 있었다. 따라서 야콘은 베타카로틴, 칼슘, 탄수화물 등 14가지 필수영양소를 함유하고 있는 알칼리성 식품으로서 향후 기능성 식품소재로도 이용가치가 높으리라 판단된다.

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Drag Characteristics According to Ground Rope and Seabeds in the Bottom Trawl (저층트롤의 발줄 및 저질의 종류에 따른 저항특성)

  • 신정욱;이주희;권병국
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.1
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    • pp.58-68
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    • 2002
  • The model experiments for the Eastern sea bottom trawl were conducted for the 3 types of ground ropes and 2 types of seabeds to examine drag characteristics according to towing speed. The model net was based on the net of trawler with 750ps and made by 1/78 of a full scale net. This experiments focused on the drags for 3 types of ground rope, the serving wire, the original and the chain types and 2 types of seabeds, mud and sand of bottom. The results obtained are summarized as follows 1. In the case of the sand bottom, the drags by 3 types of ground rope were orderly increased in turn of the chain, the serving wire and the original type. But, in the case of the mud bottom, the drag of the original type is higher than that of the serving wire type and less than the chain type. 2. In the case of the serving wire type of ground rope, the drags by the diameters of ground rope, ø 1.3, ø 1.9, ø 2.6 and ø 3.25 were increased respectively by 23g, 25g, 32g and 42g at the towing speed of 0.4m/s. 3. The drags of ground ropes were increased exponentially in proportion to the towing speed, in the same way in all of three types of ground rope. 4. The ratios of drag of ground rope against that of the trawl net by the type of ground rope at the towing speed of 0.4m/s on the mud bottom were 0.69 in the chain type, 0.64 in the serving wire type and 0.67 in the original type respectively. 5. The Coefficient of drag($C_d$) by type of ground rope according to the towing speed were 10.0~56.7, 3.0~l6.0 and 1.5~8.5 respectively in turn of the chain type, the serving wire type and the original type on the mud bottom, and that on the sand bottom were 10.0~60.0, 3.0~14.0 and 1.2~6.0 respectively In turn of the chain type, the serving wire type and the original type.

Characteristics of Traditional Mejus of Nation-Wide Collection (전국적으로 수집한 전통식 메주의 특성 조사)

  • 유진영;김현규
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.2
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    • pp.259-267
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    • 1998
  • Typical characteristics of Mejus must be understood to get the basic data for setting up mass production system of traditional fermented soybean products. One hundred and twenty one Mejus were collected from various places and analyed. Most of shapes were rectangular and some were spherical, conical, cylindrical and doughnut types. The weight of Mejus was 0.4~4.2kg. Chemical analysis showed: moisture content, 9.73~58.22% ; pH, 4.95~8.15; acidity, 0.6~3.8% ; soluble protein content, 4.45~12.31%; soluble sugar content, 0.82~10.95%. Enzyme assay showed: $\alpha$-amylase activity, 5.0~874.2 units/g; $\beta$-amylase activity, 0.02~27.74units/g; acidic protease activity, 31.3~225.1unts/g; lipase activity 1.0~53.0units/g. Total viable cells were 3.72$\times$107~1.35$\times$1010cfu/g, and yeast and mold count 6.46$\times$104~8.91$\times$106cfu/g. respectively. $\alpha$-Amylase activity of a traditional Meju from Incheon showed the highest activity of 732.8 units/g(interior section) and 823.2units/g (exterior section). $\beta$-Amylase activity was the highest{3.57 units/g (interior sectin) and 4.25units/g (exterior section)} in Meju from Chunbuk. Acidic protease activity was the highest in sample from Seoul, whereas traditional Meju from Kyongnam showed the highest activity of 21.5units/g(interior section) and 37.5units/g(exterior sectin).

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Chromium and Nickel Concentrations in Urine and Serum of None Exposed Group and Workers in Electroplating Plants (정상인과 도금업 근로자의 요 및 혈청중 크롬 및 니켈 농도)

  • Choi, Ho-Chun
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.5 no.1
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    • pp.1-7
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    • 1995
  • The exposure levels of chromium and nickel for chromeplating workers were evaluated. Chromium and nickel concentrations in urine and serum from 82 exposed workers and 69 controls, who were not exposed occupationally to metals, were analyzed by flameless atomic absorption spectrophotometry. The results were as follows: 1. Chromium concentrations in urine of exposed group and control were $3.49{\pm}1.83g/g$ of creatinine, $5.59{\pm}2.83g/g$ of creatinine, and in serum were $0.69{\pm}0.30g/l$, $2.31{\pm}1.16g/l$ respectively. There were significant difference of concentrations for chromium in urine and serum by group respectively. 2. Nickel concentrations in urine of exposed group and control were $0.92{\pm}0.23g/g$ of creatinine, $2.20{\pm}1.93g/g$ of creatinine, and serum concentrations were $0.52{\pm}0.34g/l$, $1.41{\pm}0.74g/l$ respectively. There were significant difference of concentrations for nickel in serum by groups statistically. 3. Chromium and nickel concentrations in urine and serum of exposed groups were not significant by workplaces(grinding, electroplants, packaging).

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Effect of Various Cooking Method on Pectin in Vegetables (여러 가지 조리방법에 따른 펙틴 함량의 변화)

  • Gye, Su-Gyeong
    • Journal of Applied Tourism Food and Beverage Management and Research
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    • v.17 no.2
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    • pp.27-38
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    • 2006
  • The contents and physicochemical properties of fiber in 15 kinds of vegetables being consumed commoly in Korea were investigated, and the effects of various cooking and Kimchi fermentation) on fiber were studied. Contents of total pechin in raw vegetables were 0.89-2.75g/100g, and most were 1-2g/100g, on dry weight basis. Considering each fraction of pectic substances, the content of HWSP of vegetables ranged in 0.33-0.98g/100g, HXSP, 0.29-0.81g/100g and HCLSP, 0.30-1.40g/100g. HCLSP of three pectic fractions showed the greatest variation according to the kinds of vegetables. Every vegetables showed similar contents of three pectic fraction. Cooking decreased total pectin content in all vegetables regardless of cooking methods and the greatest reduction was observed in boiling. Among three pectic fractions, hot water soluble pectin(HWSP) increased, and sodium hexamtaphosphate soluble pectin(HXSP) and HCL soluble pectin(HCLSP) decreased.

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Simultaneous Analysis for Veterinary Drug Residues in Honey by HPLC/MS/MS (HPLC-MS/MS를 이용한 벌꿀 중 동물용의약품 동시분석방법 연구)

  • Kim, Jong-Hwa;Moon, Sun-Ea;Kim, Ki-Yu;Jung, You-Jung;Lee, Chang-Hee;Ku, Eun-Jung;Yoon, Mi-Hye;Lee, Jong-Bok
    • Journal of Food Hygiene and Safety
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    • v.31 no.2
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    • pp.94-98
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    • 2016
  • This study was conducted to establish the simultaneous analysis method for veterinary drug residues in honey by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The eleven targeting veterinary drugs with honey test method in Korean Food Standards Codex were divided into Group 1 (streptomycine dihydrostreptomycine, neomycine) and Group 2 (oxytetracycline, enrofloxacin, ciprofloxacin, cymiazole, chloramphenicol, amitraz, coumaphos, fluvalinate) to be analyzed simultaneously. From the results, the retention time (RT) of the targeting drugs was within 15 min, the range of detection limits was 0.0056 to $0.0643{\mu}g/g$ and the range of quantification limits was 0.0169 to $0.1948{\mu}g/g$. The coefficients of determination ($R^2$) for Group 1 ($0.05{\sim}1.0{\mu}g/mL$) and Group 2 ($0.01{\sim}1.0{\mu}g/mL$) were 0.9917~0.9987 and 0.9923~1.000 respectively, and showed the good linearity. The recovery rates for Group 1 (final conc. $0.25{\mu}g/g$) and Group 2 (final conc. $1.0{\mu}g/g$) were 65.1~80.6% and 64.2~90.3% respectively. Also, the analysis results of inter day (n = 3) and intra day (n = 6) RSD (%) for area and retention time showed that the RSD (%) for area and retention time was below 10.92% and 1.57%. Therefore, the simultaneous analysis method of this study is evaluated to be a good test method for veterinary drug residues in honey.

The Effect of Feeding Houttuynia cordata Thunb on Cholesterol and Cholesterol oxides of Pork Lion's (어성초 분말의 혼합 급이가 돈육의 cholesterol 및 산화물에 미치는 영향)

  • Kang, Min-Jung;Joo, Jong-Chan;Shin, Jung-Hye;Choi, Sun-Young;Yang, Seung-Mi;Sung, Nak-Ju
    • Journal of Life Science
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    • v.16 no.6
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    • pp.1029-1035
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    • 2006
  • The study was designed to investigate the influence of feeding Eosungcho(houttutnia cordata Thunb) on the meat quality of porks. Experimental groups were divided into control group(C), 5%(T1) and 10%(T2) Eosungcho powder feeding group, and than administered for 12 weeks. And then pork's loins were storaged at $4^{\circ}C$, for 23 days after vaccum packed. TBARS was increased(P<0.05) during storage days in all samples, while it's contents were lower Eosungcho feding groups than control group. Total cholesterol contents of pork's loin, were $48.9{\pm}0.2\;mg/100\;g$ in control group, $39.1{\pm}0.2\;mg/100\;g$ in T1 group and $37.8{\pm}0.3\;mg/100\;g$ in T2 group. 4 kinds of cholesterol oxides($7{\beta}-hydroxycholesterol$, 19-hydroxycholesterol, ${\alpha}-$ and ${\beta}-epoxide$) detected in pork's loin. Cholesterol oxides concentration of pork's loin was increased during storage and lowest in T2 group.

Potential Use of Microalgae Scenedesmus acuminatus for Tertiary Treatment of Animal Wastewater (축산폐수 고도처리를 위한 미세조류 Scenedesmus acuminatus의 이용 가능성)

  • Park, Ki-Young;Lim, Byung-Ran;Lee, Ki-Say;Lee, Soo-Koo
    • Journal of The Korean Society of Agricultural Engineers
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    • v.53 no.1
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    • pp.63-69
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    • 2011
  • The green algae Scenedesmus acuminatus was cultured in different media: animal wastewater and an artificial culture medium in order to evaluate potential use for tertiary treatment. The experiments were conducted with air flowrate 1~2 L/min at $28{\sim}30^{\circ}C$. The nitrogen and phosphorus showed very similar removal efficiencies (68~77 % and 69~80 % for nitrogen and phosphorus respectively). The optimal fed period was estimated as three days in the semi-continuous experiment. The effects of $CO_2$ (4.5 %) injection on nutrient uptake from animal wastewater (biological treatment effluent) were compared to an air injection under the same conditions of light and photoperiod. The uptake rates of nutrient with air injection were observed 0.009 gN/gChl-a/day, 0.028 gN/gChl-a/day and T-P 0.003 gP/gChl-a/day for nitrate, total nitrogen and phosphorus respectively. The rates were enhanced by addition of $CO_2$ to 0.026 gN/gChl-a/day, 0.076 gN/gChl-a/day and T-P 0.018 gP/gChl-a/day. This study establishes that $CO_2$ addition during nutrient deprivation of microalgal cells may accelerate tertiary wastewater treatment.