• BMB Reports
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• v.44 no.9
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• pp.572-577
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• 2011

Elevated phospholipase D (PLD) expression prevents cell cycle arrest and apoptosis. However, the roles of PLD isoforms in cell proliferation and apoptosis are incompletely understood. Here, we investigated the physiological significance of the interaction between PLD2 and protein kinase CKII (CKII) in HCT116 human colorectal carcinoma cells. PLD2 interacted with the CKII${\beta}$ subunit in HCT116 cells. The C-terminal domain (residues 578-933) of PLD2 and the N-terminal domain of CKII${\beta}$ were necessary for interaction between the two proteins. PLD2 relocalized CKII${\beta}$ to the plasma membrane area. Overexpression of PLD2 reduced CKII${\beta}$ protein level, whereas knockdown of PLD2 led to an increase in CKII${\beta}$ expression. PLD2-induced CKII${\beta}$ reduction was mediated by ubiquitin-dependent degradation. The C-terminal domain of PLD2 was sufficient for CKII${\beta}$ degradation as the catalytic activity of PLD2 was not required. Taken together, the results indicate that the C-terminal domain of PLD2 can regulate CKII by accelerating CKII${\beta}$ degradation in HCT116 cells.

• Journal of the Korean Operations Research and Management Science Society
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• v.35 no.2
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• pp.1-17
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• 2010

This paper aims to compare four operating priority rules of RMGC (Rail Mounted Gantry Crane) used in semi-automated container terminal. The four priority rules employed in this paper are FCFS (First-come-first-served), LCFS (Last-come-first-served), TOS (Turn-over-served) and NFS (Nearest-first-served). And to compare the four operating priority rules, this paper analyzed productivity of RMGC and CC (Container Crane), waiting time of YT (Yard Truck) and RT (Road Truck) in container yard, and turnaround time of RT in container terminal of each priority rule by using stevedoring simulation. As a result, NFS was evaluated as the best rule to improve the overall productivity of container terminal in terms of all criteria.

• Journal of Sasang Constitutional Medicine
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• v.12 no.2
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• pp.195-200
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• 2000

For many patients suffered from a terminal cancer, the best care is to extend a period and improve a quality of life. So that the pain, a major symptom of patients with terminal cancer, should be effectively controled. Otherwise it causes anorexia, nausea, vomiting, general weakness, loss of body weight, insomnia and becomes worse the condition of patients. The case is a report about a patient diagnosed as terminal gastric cancer and suffered abdominal pain. The patient was treated by Taeumin Chongsim Yonja Tang and the abdomnal pain decreased. This report described the process and contents about the way the patient was cured.

• Journal of the Korean Home Economics Association
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• v.41 no.7
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• pp.25-38
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• 2003

This study explored the terminal values(Rokeach's Value) and 5-money spending styles of girls in Yanbian, China and those in South Korea. The subjects were 466 school girls in Yanbian, China and 498 high school girls in South Korea. We used Cronbach' α, t-test, and Pearson correlation analysis as statistical analysis. The results were summarized as follows : 1) at the top of the terminal value hierarchy of girls in the two countries were family security and happiness. 2) resulting from the t-test, there found significant differences between the 5-money spending styles of the Yanbian girls and that of the Korean girls. 3) resoling from the Pearson correlation analysis, there found similarities and differences in relationship between terminal values and money spending styles in two groups.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.175-178
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• 1993

Alkaline .alpha.-amylase expressed in the transformant, Baciollus subtills MB809, containing alkaline amylase gene cloned from alkalophilic Bacillus sp. AL-8, was purified through for step separation processes. The purified alkaline .alpha.-amylase had molecular weight of app[roximately 59, 000 daltons on SDS-PAGE and Sephaex G-100 gel filtration. Amino acid sequence of terminal portion of the enzyme was analyzed with pure amylase eluted form the SDS-PAGE gel. N-terminal amino acid sequence of .alpha.-amylase was determined by the Edman degradation method and resulted in $NH_{2}$-ser-thr-ala-pro-ser-(ile)-lys-ala-gly-thr-(ile)-leu. For C-terminal amino acid sequencing, purified .alpha.-amylase was digested with carboxypuptidase A and B, and reverse-phase HPLC gradient elution system resulted in -thr-trp-pro-lys-COOH.

• Proceedings of the Korean Statistical Society Conference
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• 2003.10a
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• pp.73-78
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• 2003

This presentation derives a distribution function of the terminal value and running maximum of two-dimensional Brownian motion {X(t) = (X$_1$(t), X$_2$(T))', t > 0}. One random variable of the joint distribution is the terminal time value of the Brownian motion {X$_1$(t), t > 0}. The other random variable is the partial-time running maximum of the Brownian motion {X$_2$(t), t > 0}. With this distribution function, this presentation also derives an explicit pricing formula for a barrier option whose monitoring period of the option starts at an arbitrary date and ends at another arbitrary date before maturity.

• Journal of Korean Association for Spatial Structures
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• v.15 no.4
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• pp.49-56
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• 2015

The roof grid of single-layer space frame structure, for Energy Core of Incheon Airport Second Terminal, is very simple and aesthetic, but it is apt to buckle under external force because of mild curvature and complex shape. The object of this study is to estimate the stability of single-layer space frame structures for Energy Core of Incheon Airport Second Terminal with the analytical conditions of structural design. The results show that the buckling load of model(pin-pin, uniform load, rigid joint), that is, the most similar model to the analytical conditions of structural design. was $10.7kN/m^2$.

• Journal of Navigation and Port Research
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• v.29 no.2
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• pp.165-173
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• 2005

As the international society becomes more and more open, the international marine transportation demand is growing. And the growth of international tour demands and the developments of new passenger ship routes make the role of passenger terminals crucial. In this context this study analyzes and compares the terminals of Korea and Japan and defines design criteria for the construction of a passenger terminal. As a result of this study, it is proposed that an urban context, an architectural type and spatial characteristics be taken into account for the construction cf a passenger terminal. The ultimate purpose cf this study is to build a future-oriented international passenger terminal based on the result of this study, which will lead the ocean culture.

• Journal of Navigation and Port Research
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• v.44 no.2
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• pp.88-97
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• 2020

In the past 20 years, passenger and cargo transportation performance at the international passenger ports in Korea have increased by 8.6% on the compound annual growth rate, respectively. The Korean government had expected to continue expanding the market of car ferries and the government included plans for the development of additional routes and lanes with neighboring countries such as China, J apan, and Russia as a part of the Plan of the Korean Ministry of Oceans and Fisheries in 2019. However, compared to the development of routes, the management and operation of passenger terminals are inefficient. The purpose of this paper was to introduce the overall improvement of the passenger terminal operating system that reflects the recent changes in the supply and demand of international passenger vessels and the needs of the government and private sector passengers. To this end, a basic survey was conducted on the status of operations and facilities at the Busan International P assenger Terminal and a questionnaire survey was administered on the subject of use. The results of the survey were analyzed using the IPA model. As a result of the analysis, three items in the operation sector, three items in the facility sector, and four items in the system sector of the Busan International Passenger Terminal were identified as in need of improvement. Based on the results, the need for investment to improve the international passenger terminal operating system is suggested.

• BMB Reports
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• v.33 no.6
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• pp.531-536
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• 2000

Tropomyosin (TM) is an important actin binding protein involved in regulation of muscle contraction. Unacetylated striated tropomyosin failed to bind to actin whereas unacetylated smooth tropomyosin bound well to actin. It has been demonstrated that high actin affinity of unacetylated ${\alpha}-tropomyosin$ was ascribed to the carboxyl terminal amino acid residues. In order to define the role of the carboxyl terminal residues of tropomyosin molecule on actin binding, two mutant tropomyosins were constructed. TM11 is identical to the striated tropomyosin except that the carboxyl terminal last three amino acids was replaced with $^{282}NNM^{284}$ whereas in TM14 $^{276}HA^{277}$ was substituted with smooth specific $^{276}QT^{277}$. TM11 and TM14 were overproduced in Escherichia coli and analyzed for actin affinity. The apparent binding constants (Kapp) of unacetylated tropomyosins were $2.2{\times}10^6M^{-1}$ for sm9, $1.03{\times}10^6M^{-1}$ for TM14, $0.19{\times}10^6M^{-1}$ for TM11, $>0.1{\times}10^6M^{-1}$ for striated, respectively. This result indicated that higher actin affinity of the unacetylated smooth tropomyosin was primarily attributed to the presence of QT residues in the smooth sequence. In case of the Ala-Ser (AS) dipeptide extension of the amino terminus of tropomyosin, Kapp were $21.1{\times}10^6M^{-1}$ for AS-sm9, $8.0{\times}10^6M^{-1}$ for AS-11, $4.7{\times}10^6M^{-1}$ for AS-14, $3.8{\times}10^6M^{-1}$ for AS-striated. AS-TM11 showed considerably higher actin affinity than AS-TM14, implying that interaction of Ala-Ser of the amino terminus with the carboxyl terminal residues. Since Kapp of AS-TM11 was significantly lower than that of AS-sm9, the presence of QT might be required for restoration of high actin affinity of the smooth ${\alpha}-tropomyosin$. These results suggested that the carboxyl terminal amino acid residues Glutamine275-Threonine276 are important for actin affinity of the recombinant smooth ${\alpha}-tropomyosin$, particularly of unacetylated smooth ${\alpha}-tropomyosin$.