• 동서간호학연구지
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• 제27권2호
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• pp.124-133
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• 2021

Purpose: This study examined the effects of death perception, terminal care stress, and resilience on the terminal care performance of clinical nurses. Methods: As a correlational study, this study conducted a survey total of 189 nurses working at K university hospital and a P university hospital in B metropolitan city for six months or more. The data was collected from August 20th, 2020 to September 15th, 2020. Data were analyzed using the SPSS, Windows version 25.0, according to the purpose of the analysis by descriptive statistics, t-test, ANOVA, Scheffe test, Pearson's correlation coefficient, and hierarchical multiple linear regression. Results: The factors having effects on the terminal care performance of subjects were resilience, perception of death, and the experience of terminal care education. The total explanatory power of those variables on the terminal care performance was 21.0%. Conclusion: For the improvement of terminal care performance, it would be necessary to develop a systematic and professional terminal care education program that could increase the positive perception of death and resilience, and also include knowledge and skills necessary for terminal care. Also, the efficiency of terminal care performance should be increased through periodic terminal care-related nursing education.

• 중환자간호학회지
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• 제9권2호
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• pp.61-70
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• 2016

Purpose: The purpose of this study was to identify the influences of death perception, terminal care attitude on clinical nurses' terminal care performance for cancer patients. Methods: Data were collected through self-reported questionnaires filled by 526 nurses at a General Hospital in Seoul. Data were analyzed using a multiple regression analysis. Results: Death perception showed a positive correlation with terminal care attitude (r = .45, p < .001), while there was no correlation with terminal care performance. Additionally, terminal care attitude had a positive correlation with terminal care performance (r = .18, p < .001). The explanatory power of nurses' death perception and terminal care attitude toward terminal care performance was 14%. Conclusions: The study results imply that nurses' death perception and terminal care attitude are significant variables affecting terminal care performance.

• 전기학회논문지
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• 제62권12호
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• pp.1798-1802
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• 2013

We present a technique for measuring low resistance ranges of a decade resistor with a 4-Terminal connection. With the technique the accuracy of 0.8 % was obtained for a 1 $m{\Omega}$ resistance of the decade resistor. We suggested a proper pattern 4-Terminal measurement results with several 4-Terminal pattern and adapters. Additionally, we should that precise measurements for low resistance can be made usung a digital multimeter(DMM) only.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.41-41
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• 2003

Small conductance calcium-activated potassium channels (or SKCa channels) are potassium selective, voltage-independent, and activated by intracellular calcium concentration. These channels play important roles in excitable cells such as neuron in the central nervous system (Vergara et al., 1998). The activity of SKCa channels underlies the slow afterhyperpolarization that inhibits neuronal cell firing (Hille, 1991; Vergara et al.,1998). Until now, N-terminal region of rSK2 isn't characterized. To study the role of N-terminus, we constructed the N-terminal deletion mutant and characterized by electrophysiological means. Interestingly, N-terminal deletion mutant be trafficked to membrane couldn't evoke any ionic currents. Thus, N-terminal region has a role in functional rSK2 channel formation. To elucidate the function of N-terminal region, (His)6-conjugated protein was purified and filtrated by affinity column chromatography. Surprisingly, N-terminal region was shown in tetramer size that was supported by cross-linking result. Thus, we predicted that N-terminal region might be involved in the tetramerization of rSK2.

• BMB Reports
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• 제56권3호
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• pp.172-177
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• 2023

BEST family is a class of Ca²⁺-activated Cl- channels evolutionary well conserved from bacteria to human. The human BEST paralogs (BEST1-BEST4) share significant amino acid sequence homology in the N-terminal region, which forms the transmembrane helicases and contains the direct calcium-binding site, Ca²⁺-clasp. But the cytosolic C-terminal region is less conserved in the paralogs. Interestingly, this domain-specific sequence conservation is also found in the BEST1 orthologs. However, the functional role of the C-terminal region in the BEST channels is still poorly understood. Thus, we aimed to understand the functional role of the C-terminal region in the human and mouse BEST1 channels by using electrophysiological recordings. We found that the calcium-dependent activation of BEST1 channels can be modulated by the C-terminal region. The C-terminal deletion hBEST1 reduced the Ca²⁺-dependent current activation and the hBEST1-mBEST1 chimera showed a significantly reduced calcium sensitivity to hBEST1 in the HEK293 cells. And the C-terminal domain could regulate cellular expression and plasma membrane targeting of BEST1 channels. Our results can provide a basis for understanding the C-terminal roles in the structure-function of BEST family proteins.

• Journal of Hospice and Palliative Care
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• 제19권2호
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• pp.154-162
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• 2016

목적: 본 연구는 요양병원 간호사의 임종간호수행과 죽음불안, 자아존중감 및 임종간호스트레스간의 상관관계를 확인하고 임종간호스트레스에 영향을 미치는 요인을 파악하기 위한 서술적 조사연구이다. 방법: K시와 B광역시 소재 10개 요양병원에 근무하는 212명의 간호사를 대상으로 자가 보고식 설문지를 이용하여 자료를 수집하였다. 결과: 요양병원간호사의 임종간호스트레스에 영향을 미치는 요인은 죽음불안과 임종간호수행이었으며 죽음불안과 임종간호수행 정도가 높을수록 임종간호스트레스가 높았다. 이들 변수들은 임종간호스트레스에 대하여 32.5%의 설명력을 나타냈다. 결론: 요양병원간호사의 임종간호스트레스를 완화시키기 위하여 효율적인 인력배치 등의 행정적인 방안을 수립하고 죽음불안을 감소시킬 수 있는 임종간호교육프로그램 개발 및 효과검증 연구 수행을 제안하는 바이다.

• 생명과학회지
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• 제6권3호
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• pp.185-192
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• 1996

박테리오파지 T7 gene 2.5 단백질은 single-stranded DNA 결합 단백질로 박태리오파지 T7의 DNA복제, 재조합, 및 수선에 필수적으로 요구된다. Gene 2.5 protein은 T7의 DNA 합성과 성장에 필수적인 단백질이다. Gene 2.5 Protein이 중요시 되는 이유는 이 단백질이 T7의 다른 복제 필수단백질인 T7의 다른 복제 필수단백질인 T7 DNA polymerase 와 gene 4 protein(helicase/primase)와 서로 상호작용할 것으로 제안되었기 때문이다. (Kim and Richardson, J. Biol. Chem., 1992;1994). 이 단백질의 단백질 상호작용을 가능하게 하는 domain은 carboxyl-terminal domain일 것으로 여러 실험에서 대두되었기에, 이 domain의 특성을 파악하기 위해 야생형과 변이체 gene 2.5 단백질들을 각각 GST에 융합한후 fusion 단백질을 정제하였다. 정제된 이 융합 단백질들의 carboxyl-terminal domain이 T7 복제 단백질들과 상호작용을 조사하는지를 조사하기 위해 affinity chromatography로 이용하였다. 실험 결과, 아생형 GST-gene 2.5 융합단잭질(GST-2.5 (WT))는 T7 DNA polymerase 와 상호작용을 하였지만. 변이형 융합단백질(GST-2.5$\Delta$21C)는 interaction을 하지 못했다. 이 결과는 carbohyl-terminal domain이 단백질-단백질 상호작용을 하는데 직접적으로 관여하는 것을 증명하였다. 또한,GST2.5(WT)는 gene 4 protein(helicase/primase)와 직접 상호작용을 하나. GST2.5$\Delta$21C는 상호작용을 하지 못하는 것으로 나타났다. 따라서 gene 4 proteins와의 상호작용에도 gene 2.5 protein의 carboxyl-terminal domain이 직접 관여 한다는 것이 증명되었다. 이상의 결과에서 gene 2.5 protein은 박테리오파지 T7 의 유전자 목제 시 단백질-단백질 상호작용에 관혀아며, 특히 gene 2.5 protein의 carboxyl-terminal domain이 이러한 상호작용에 직접적으로 관여하는 domain이라는 것을 알 수가 있었다.

• 한국항해항만학회지
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• 제33권2호
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• pp.153-159
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• 2009

The awareness of the high-value industry for container terminal leads competitiveness of container terminals to keep high fiercely. In regards to competitive factors of container terminal, the most important point among several factors is seemed to be the speed of container loading and unloading on quayside. In container terminals in Korea, the productivity shows big difference even though its condition is similar to each terminal. The objective of this paper is to find the critical factors of container terminal productivity, which is dependant upon the capability, quantity of quay crane, transfer vehicle, and so on. For this purpose, we have researched related literatures, and collected data about container terminals in South Korea. Furthermore, we tested sensitive analysis to evaluate the extent of productivity by changing independent variable. And then we established the regression model to evaluate which factor has had the biggest impact on productivity. The results of this paper can give terminal operators guideline to improve productivity.

• 한국농촌건축학회논문집
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• 제17권3호
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• pp.27-36
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• 2015

This research studies the adequate size standard of Seogwipo cruise terminal CIQ facility that is scheduled to be built around Gangjeong harbor area in Seogwipo-city. In order to respond to the highly increasing number of passenger cruise ships compared to Seogwipo cruise terminal design in 2010, the adequate size standard of Seogwipo cruise terminal CIQ facility was examined for passenger service level grade. Based on size computation elements such as the number of passengers of cruise ships with the largest size of port entry, ship landing rate, passenger processing ratio, and surge factor, the CIQ facility size for each service level grade was reviewed. As a result, the area of 2,971m2 (A grade), 2,409 m2 (B grade), and 2,088 m2 (C grade) were computed. This showed that the area of B grade was about 82% and C grade 70% compared to the area of A grade. The CIQ facility size computed for each service level grade in this research was analyzed that its area needed to be increased by 322% at least and 458% at most, compared to the CIQ facility area of 649m2 of the existing design (2010). In order to respond to the increasing number of cruise passengers, provide high-level passenger service, and improve the international image of Jeju, Seogwipo cruise terminal should secure the size that is equal to or higher than the B grade of service level.

• 한국보건간호학회지
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• 제37권2호
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• pp.275-289
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• 2023

Purpose: This study developed a structural model for explaining and predicting terminal care performance in long-term care hospital nurses. The model was based on the stress integration model of Ivancevich and Matteson(1980) and the results of previous studies. Method: Data was obtained from August to September 2022 from 267 nurses in 13 long-term care hospitals in G-do. Results: Results of model verification for this study, revealed that factors directly affecting the terminal care performance of long-term care hospital nurses were nursing work environment(β=0.43, p<0.001), death anxiety(β=-0.29, p<0.001), and terminal care stress(β=0.22, p=0.003). However, the attitude toward nursing care of dying(β=0.07, p=0.287) had no effect on the terminal care performance. Conclusion: The results of this study, confirmed the necessity of improving an individual's perceived nursing work environment, continuous education related to terminal care to reduce death anxiety, and an interventional approach for enhancing terminal care performance.