• Title/Summary/Keyword: 2차 압

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Effects of Culture Medium and Osmolarity on In Vitro Maturation of Follicular Oocytes and Development of Parthenogenetic Embryos in Porcine (배양액과 삼투압이 돼지 난포란의 성숙과 단위발생란의 발육에 미치는 영향)

  • Kim M. K.;Kwon D. J.;Park C. K.;Yang B. K.;Cheong H. T.
    • Reproductive and Developmental Biology
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    • v.29 no.3
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    • pp.169-174
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    • 2005
  • This study was comducted to examine the effects of culture medium, and the osmolarity and osmotic change of the culture medium on in vitro maturation of porcine oocytes and developement of porcine parthenogenetic embryos. In Experiment 1, cumulus-oocyte complexes were matured in NCSU-23, mWM and mKRB, respectively, There was no difference in maturation rate($62.1\~71.3\%$) among groups. In Experiment 2, matured oocytes in each medium were activated and cultured for 6 days in the same media. Blastocyst formation rate was higher in NCSU-23($22.9\%$) than those of others($0\~0.6\%$, P<0.05). In Experiment 3, parthenogenetic embryos were cultured for 6 days in NCSU-23 with different osmolarity(300, 280 and 256 mOsmols) adjusted by NaCl. There were no differences in development rates to the blastocyst stage($11.0\~14.4\%$) among groups. In Experiment 4, activated oocytes were cultured for 2 days in NCSU-23 with 300, 280 and 256 mOsmols and then transferred to increased or decreased osmotic condition. Blastocyst formation rate was higher in a group which was transferred from the higher osmotic condition to the lowe. osmotic condition($21.0\%$) than a contrary group( $11.8\%$, (P<0.05). This result shows that the culture medium and the osmolarity of the culture medium affect the development of porcine parthenogenetic embryos, and the change of osmolarity from the higher condition to the lower condition at a certain developmental stage can enhance the development of porcine parthenogenetic embryos.

Influence of Dietary Supplemental Betaine on Performance and Egg Quality of Laying Hens during the Heat Stress (고온 스트레스기에 비태인의 사료첨가가 산란계의 생산성 및 계란의 품질에 미치는 영향)

  • 류경선;류명선;신원집;조기행
    • Korean Journal of Poultry Science
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    • v.29 no.2
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    • pp.117-124
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    • 2002
  • Betaine functions as an osmoregulators in the cells and its inclusion in diet can spare the choline and carcass fat reduction in chicken. Thus, two hundred eighty eight laying hens were fed with 0, 500, 1,000, 2,000 ppm of betaine from seventy eight to eighty six weeks of age during the environmentally high temperature stress. Com and soybean basal diets contained 2,800 kcal/kg ME and 16% CP. Egg production, feed intake, and feed conversion were examined for eight weeks. Egg quality characters, serum cholesterol, liver betaine, and lower ileal osmolality were measured at the end of experiment. Egg Production rates of hens fed with 500 or 2,000 ppm of betaine were 75.06 and 75.02%, respectively and tended to increase compared to the control. The feed conversion(FCR) of these treatments was significantly(P<0.05) improved compared to that of control although it did not significantly differ in the e99 Production rates between 500 and 2,000 ppm of betain groups. Eggshell breaking strength of hens fed betaine was significantly(P<0.05) higher than those of control. However, betaine supplements did not influence to improving the albumen height and Haugh unit. Liver betaine in hens fed with betaine was linearly increased unto 2,000 ppm. The birds fed with 2,000 ppm betaine showed significantly(P<0.05) higher in the liver betaine than the control birds. Total cholesterol and triglyceride tended to be increased by dietary betaine supplement. The lower ileal osmolality in betaine supplement group tended to be slightly decreased. As a result, dietary betaine supplement tended to improve the egg Production and eggshell Quality in laying hens during heat stress.

The Effects of Nitric Oxide Inhibitor on Hyperdynamic Circulation in Portal Hypertensive Rats (산화 질소 억제제가 문맥 고혈압 쥐의 혈역학 변화에 미치는 영향)

  • Kim, Pill-Young;Jang, Byeong-Ik;Kim, Tae-Nyeun;Chung, Moon-Kwan
    • Journal of Yeungnam Medical Science
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    • v.16 no.2
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    • pp.181-192
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    • 1999
  • Background: Nitric oxide, a vasodilator synthesized from L-arginine by vascular endothelial cells, accounts for the biological activity of endothelium derived relaxing factor. Previous studies demonstrated that nitric oxide inhibitor, $N^{\omega}$-Nitro-L-Arginine(NNA) diminished the hyperdynamic splanchnic and systemic circulation in portal hypertensive rats The present study was done to determine the role of nitric oxide in the development of hyperdynamic circulations in the prehepatic portal hypertensive rat model produced by partial portal vein ligation. Methods: The portal hypertensive rats were divided into water ingestion group and NNA ingestion group. After partial portal vein ligation, NNA ingestion group and water ingestion group received NNA 1mg/kg/day and plain water through the mouth for 14 days, respectively. Cardiac output, mean arterial pressure, organ blood flow and porto-systemic shunting were measured by radioisotope labeled microsphere methods. Vascular resistances were calculated by standard equation. Results: There were significant decreases in mean arterial pressure, increases in cardiac output and cardiac index, and decreases in total systemic and splanchnic vascular resistance in portal hypertensive rats compared to normal control group (p<0.01). Compared to the water ingestion group, significantly increased mean arterial pressure with decreased cardiac output and cardiac index were developed in the NNA ingestion group. Total systemic and splanchnic vascular resistance were significantly increased in the NNA ingestion group compared to water ingestion group (p<0.05). But, there was no significant difference in portal pressure between the two groups. Conclusion: The hemodynamic results of this study indicate that hyperdynamic circulation in prehepatic portal hypertensive rat mode1 was attenuated by ingestion of NNA. Nitric oxide may play an important role in the development of hyperdynamic circulation with splanchnic vasodilation in chronic portal hypertension.

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A Study on the Stability of the Accelerating Voltages in Scanning Electron Microscopy (주사전자현미경에서 가속전압의 안정성 연구)

  • Bae, Moon-Seob;Oh, Sang-Ho;Cho, Yang-Koo;Lee, Hwack-Joo
    • Applied Microscopy
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    • v.34 no.1
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    • pp.51-59
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    • 2004
  • The high acceleration voltage system used in scanning electron microscope were designed and manufactured to test its stability. The Cockcroft-Walton circuits are used both in the cathode voltage up to -30 kV and in the Wehnelt cylinder of -2 kV. The operating voltage of 6 V was applied to the heating of the filament. The wave forms which are formed in the second leg of the high voltage transformer were observed in the oscilloscope with 2 V of DC input. When the high voltages were in the range between 5 kV and 12 kV, the highest value of the stabilities of the generated voltages was obtained as 0.002%.

Development of 3kW LDC for High Efficiency using SiC for EV BUS (SiC를 이용한 전기버스용 3kW 고효율 저전압 전력변환장치 개발)

  • Kang, Min-Hyuck;Jung, Eun-Jin;Kang, Chan-Ho;Lee, Byoung Kuk
    • Proceedings of the KIPE Conference
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    • 2016.07a
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    • pp.223-224
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    • 2016
  • 본 논문은 상용급 전기버스의 24 V 전장전력공급장치로써 고전압배터리부터 저전압으로 변성하는 전력변환장치인 저전압 직류변환장치 (Low Voltage DC/DC Converter : LDC) 개발에 관하여 기술한다. 제안하는 LDC는 효율을 높이기 위해 트랜스포머 1차 측 위상천이 전브리지 스위칭 소자에 SiC MOSFET을 사용하고, 2차 측에 동기정류방식을 적용하였다. 고효율 성능을 검증하기 위해 시작품을 제작하고 시험을 통해 3 kW 97% 이상의 고효율, 고출력, 고밀도의 특성을 확인하였다.

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Fabrication and Performance of $Bi_{0.5}Sb_{1.5}Te_{3}/Bi_{2}Te_{2.4}Se_{0.6}$ Thin Film Thermoelectric Generators ($Bi_{0.5}Sb_{1.5}Te_{3}/Bi_{2}Te_{2.4}Se_{0.6}$계 박막형 열전발전 소자의 제작과 작동 특성)

  • Kim Il-Ho;Jang Kyung-Wook
    • Journal of the Korean Vacuum Society
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    • v.15 no.2
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    • pp.180-185
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    • 2006
  • Microwatt power level at relatively high voltage(order of volt) was produced by $Bi_{0.5}Sb_{1.5}Te_{3}/Bi_{2}Te_{2.4}Se_{0.6}$ thin film thermoelectric generators, and maximum output power varied with temperature difference in the square-law relation. Output voltage and current were possible to control by changing the way of electrical connection as well as the number of stacking plate-modules. Variation of open circuit voltage and short circuit current with temperature difference showed a linear relationship. There were, however, some differences in variations; open circuit voltage were dependent on the number of plate-module when connected in series, but it was not for parallel connection. On the other hand, short circuit current showed the opposite behavior to the case of open circuit current.

A Study on Portable Multi Electrical Cupping System (휴대용 멀티 전동 부항 시스템에 관한 연구)

  • Kim, Jong-Chan;Yang, Chul-Oh;Ban, Kyeong-Jin;Lee, Kwang-Eui;Kim, Chee-Yong
    • Journal of Korea Multimedia Society
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    • v.15 no.2
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    • pp.250-257
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    • 2012
  • Recently oriental medicine has become a new issue in the global medical field because of increasing demands for the treatment of old people. Western medical equipment is becoming popular with big companies related to IT, communication and medical treatment. But globalization of oriental medicine techniques is inadequate due to lack of knowledge about oriental medicine. The cupping equipment has great growth potential because it is easy for old people to use the equipment. The purpose of this paper is to promote the portable multi electrical cupping system. The system may be used by patients themselves without any help from others. By using many cupping cups at the same time and regulating the timer and vacuum pressure. The patient can decrease itching and relax the hardened muscles. The system is expected to grow in the future as oriental medicine will be in high demand in aging society with IT convergence industry.

A Study on the Optimum of Closed ${CO}_{2}$ Gas Turbine Process for Nuclear Energy Power Plant(I) (원자력 발전소에 대한 밀폐 ${CO}_{2}$ 가스터빈 프로세스의 최적화 연구 I)

  • 이찬규;이종원
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.13 no.3
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    • pp.490-499
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    • 1989
  • These days the closed cycle gas turbine attracts considerable attention due to : (1) The possibility of directly coupling the closed cycle gas turbine with a high temperature gas cooled reactor ; (2) the economical use of dry coolers to reduce the thermal charge of the environment ; and (3) the reduction of pollution and energy consumption, by replacing the domestic hearth by a central heating and power station. In this paper, we selected the optimal cycle from the characteristic of thermodynamic cycle for the optimal design of closed CO$_{2}$ gas turbine cycle usuable in nuclear energy power plant. Also the effects of between the parameters and thermal efficiency were investigated by computer simulation. These results and design data will be added to basics in optimal designing closed CO$_{2}$ cycle gas turbine plant.

Effect of Glycopyrrolate on Cardiovascular System in Dogs Sedated with Medetomidine-Midazolam Combination (개에서 Medetomidine과 Midazolam 병용 투여 시 Glycopyrrolate가 심맥관계에 미치는 영향)

  • Han, Dae-Kyung;Shin, Beom-Jun;Lee, Jae-Yeon;Jee, Hyun-Chul;Park, Ji-Young;Kim, Myung-Cheol;Jeong, Seong-Mok
    • Journal of Veterinary Clinics
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    • v.26 no.4
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    • pp.317-323
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    • 2009
  • This study was performed to evaluate the effect of intravenous administration of glycopyrrolate on cardiovascular and respiratory system in dogs given intravenous medetomidine (20 ${\mu}g$/kg) and intramuscular midazolam (0.3 mg/kg) (MM). Prior to administration of MM, glycopyrrolate was administered intravenously at doses of 5 ${\mu}g$/kg (Gly-5), 10 ${\mu}g$/kg (Gly-10) or 20 ${\mu}g$/kg (Gly-20), respectively. For the control group saline was administered intravenously. In the cardiovascular system, HR, BP, RAP, PAWP, CI, SI, SVR, and PVR were measured. RR, $V_T$, $P_{ETCO2}$, and arterial blood gas analysis were measured for respiratory system. Although rapid and satisfied depth of sedation was obtained by MM, life-threatening bradycardia, the outstanding side-effect on cardiovascular system in dogs were observed. This combination also decreased CO and increased SVR, RAP, and PAWP significantly. The bradycardia could be prevented in all the glycopyrrolate treated groups, but tachycardia was observed in Gly-10 and Gly-20 groups. Significant increases in blood pressure were shown in glycopyrrolate treated groups. Also, tachycardia depends on dose of glycopyrrolate, compensating the CO. However, these were not fully reserved. In conclusion, MM combination could induce rapid and satisfied depth of sedation but was not the suitable method for the deep sedation of dogs with cardiovascular or circulatory problems.

Ring Shear Characteristics of Two Different Soils (이질 재료 간의 링 전단특성 연구)

  • Park, Sung-Sik;Jeong, Sueng-Won;Yoon, Jun-Han;Chae, Byung-Gon
    • Journal of the Korean Geotechnical Society
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    • v.29 no.5
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    • pp.39-52
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    • 2013
  • The shear stress characteristics of landslide materials can be affected by various factors. We examined the shear stress characteristics of two different soils using ring-shear apparatus, in which saturation-consolidation-shearing speed can be easily controlled. This paper presents (i) shear stress-time characteristics, (ii) shear stress depending on normal stress and shear speed and (iii) shear stress as a function of shearing speed. Materials used in this paper were the Nakdong River sand and muds taken from Jinhae coastal area in Korea. Samples were prepared in three types: Sand (upper)-Sand (lower), Clay (upper)-Clay (lower) and Sand (upper)-Clay (lower). The upper and lower indicate the samples placed in upper and lower ring shear boxes, respectively. For given normal stresses (50 and 100 kPa) and shearing speed (0.1 mm/sec), we performed ring shear tests. Then the failure lines were determined in the second test. Last, we determined the shear stress characteristics depending on different shearing speeds, such as 0.01, 0.1, 1, 10, 100 mm/sec. As a result, we found that shear stress characteristics are strongly dependent on above three factors. The shear stress of Sand (upper)-Clay (lower) is smaller than that of Sand (upper)-Sand (lower), but slightly larger than that of Clay (upper)-Clay (lower). The shear stress is also characterized by grain crushing and wetting process at slip surface.