• Food Science and Preservation
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• v.12 no.2
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• pp.156-160
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• 2005

To obtain basic data utilizing chestnuts as a raw food material, proximate analysis was conducted. Chemical component of chestnut flesh were $63.60\%$ moisture, $118\%$ ash, $3.02\%$ crude protein, $0.615\%$ crude fat, $1.21\%$ crude fiber, and $30.37\%$ nitrogen free extract, respectively. The weight ratio of tegmen, seed coat and flesh of chestnut sample were 17.05, 14.9, and $68.05\%$, respectively. The total amino acid contents of flesh and seed coat were $2,994\;mg\%$ and $1,450\;mg\%$, respectively. The total amount of free amino acids was less than that of total amino acids. As results of mineral analysis, the content of K was higher than that of any other minerals. The contents of maltose and sucrose were higher than those of fructose and glucose. The total polyphenol contents of tegmen, seed coat, fresh, leaf and bark were $9.56\;mg\%$, $0.047\;mg\%$, $0.23\;mg\%$, $15.44\;mg\%$ and $17.85\;mg\%$, respectively.

• Journal of Life Science
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• v.24 no.9
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• pp.995-1000
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• 2014

The studies of marine viruses in terms of viral isolation and detection have been limited due to the high mutation rate and genetic diversity of marine viruses. Of the modern methods currently used to detect marine viruses, serological methods based on enzyme-linked immunosorbent assay (ELISA) are the most common. They depend largely on the quality of the antibodies and on highly purified suitable antigens. Recently, a new experimental system for using viral capsid protein as an antigen has been developed using the yeast surface display (YSD) technique. In the present study, the capsid protein gene of the red-spotted grouper nervous necrosis virus (RGNNV) was expressed and purified via YSD and HA-tagging systems, respectively. Two regions of the RGNNV capsid protein gene, RGNNV1 and RGNNV2, were individually synthesized and subcloned into a yeast expression vector, pCTCON. The expressions of each RGNNV capsid protein in the Saccharomyces cerevisiae strain EBY100 were indirectly detected by flow cytometry with fluorescently labeled antibodies, while recognizing the C-terminal c-myc tags encoded by the display vector. The expressed RGNNV capsid proteins were isolated from the yeast surface through the cleavage of the disulfide bond between the Aga1 and Aga2 proteins after ${\beta}$-mercaptoethanol treatment, and they were directly detected by Western blot using anti-HA antibody. These results indicated that YSD and HA-tagging systems could be applicable to the expressions and purification of recombinant RGNNV capsid proteins.

• Proceedings of the Korean Society of Life Science Conference
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• 2000.12a
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• pp.20-26
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• 2000

호흡기계 및 소화기계에 감염된 전염성 바이러스에 대한 역학적 기초자료로 이용하고자 1999년 1월부터 12월까지 부산지역에서 분리된 전염성 바이러스의 특징과 계절적 발생추이, 환자의 성별, 연령별 발생에 대해 조사한 결과는 다음과 같다. 1. 1999년도 바이러스 가검물 2261건에서 분리한 호흡기계감염 바이러스 279건과 소화기계 감염 바이러스 83주를 분리하였으며, 이중 인플루엔자 바이러스 A형이 96주(29.6%), B형이 107주(33.0%)로 대부분을 차지하였다. 2. 1999년의 바이러스 분리의 성별 분포는 총 360명의 환자 중 179명(49.7%)의 남성 및 181명(50.3%)의 여성으로 비슷한 양상을 나타내었다. 이중 호흡기계의 경우 279명의 감염환자 중 남성이 130명(46.6%), 여성이 149명(53.4%)으로 여성의 감염율이 비교적 높았으나, 소화기계의 경우 83명의 감염환자 중 남성이 51명(61.4%), 여성이 32명(38.5%)으로 남성의 감염율이 거의 2배정도 높게 나타났다. 3. 1999년의 연령별 분포는 10세 이하의 어린이가 194명(59.9%)으로 대부분을 차지하였으며, 이중 인플루엔자 바이러스가 99명(30.6%)으로 가장 높은 감염을을 나타내었다 유행성이하선염 바이러스의 감염어린이 중에 $l1{\sim}15$세의 연령층이 15명으로(53.3%)로 가장 높게 나타났다. 4. 1999년 월별 감염율은 호흡기계 감염증 바이러스의 경우 1월부터 4월까지, 그리고 12월에 증가 추세를 보이면서 4월에 가장 높은 감염율을 나타내었다 소화기계 감염증 바이러스의 경우 9, 10, 11월을 제외한 모든 월별에 관찰되었으며, echo와 coxsackie 바이러스는 무균성 수막염 환자에서 하절기에 집중적으로 발생하였다. 동절기에 유행하는 설사 바이러스는 12월에 비교적 높은 양상을 나타내었다. 5. 인플루엔자 바이러스는 MDCK 세포에서, 아데노 바이러스와 유행성 이하선염 바이러스는 HEp-2 세포에서, 파라인플루엔자 바이러스는 Vero 세포에서, 그리고 echo, coxsackie B 바이러스와 장내 바이러스는 HEp-2, Vero, BGM 세포에서 뚜렷한 세포병변 효과를 나타내었다. 6. 분리한 바이러스는 전자현미경으로 관찰한 결과 인플루엔자 바이러스 A 형(HINI, H3N2)은 95nm, B 형은 70nm크기의 구형을 나타내었으며, 바이러스표면의 지질 이중층이 뚜렷하게 관찰되었다. 아데노 바이러스는 외피가 관찰되지 않았으며, nucleocapsid는 symmetry이고 크기는 71nm로서 바이러스 입자 표면에 icosaheral capsomer의 배열이 명확하게 관찰되었고, 파라인플루엔자 바이러스와 유행성 이하선염 바이러스는 외피가 있는 구형의 큰 viron으로180, 170nm 크기이었다. 7. Echo와 coxsackie B group 바이러스는 모두 외피가 없는 isometric 형으로 크기는 $30{\sim}45nm$ 이었고, enteric adeno 바이러스는 84nm 크기로서 외피가 없고, 입자 표면에 capsomer의 배열이 명확하게 관찰되었고, rotavirus는 크기가 70nm이며 외층 capsid 단백질과 내층 capsid 단백질이 두층으로 되어 있는 전형적인 수레바퀴 모양을 나타내었다. 이상의 결과로 보아 호흡기계 및 소화기계에 감염되는 전염성 바이러스는 연중 지속적으로 분리되고 있으며 전염성이 강하여 집단 발생은 일으키는 경우도 많고 최근 들어 유행성 이하선염과 흥역 바이러스의 발생률이 높은 추이를 나타내고 있지만 아직은 특이한 바이러스 치료제가 개발되어 있지 않았으므로 지속적인 대책과 아울러 장기적인 발생 가능에 대한 예방책을 흥보하여야 할 것으로 보이며 계속적인 바이러스성 전염병 유행예측조사 및 역학조사가 적극적으로 이루어져야 할 것으로 사료된다.

• Proceedings of the KIEE Conference
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• 2002.07c
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• pp.1437-1439
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• 2002

실리콘 고무는 내열성, 내후성, 발수성, 내오염성, 내트래킹성 등이 우수하여 옥외절연물용의 외피 재료로 널리 사용되고 있다. 폴리머 애자에서 전기방전 (코로나, 트래킹 등)에 의한 외피표면의 발수성 저하가 절연물의 장기성능에 있어서 가장 중요한 인자들 중에 하나이다. 실리콘 고무에는 ATH가 다량 첨가되는데 ATH의 첨가량, 표면처리상태, 입도 등에 의해 실리콘 고무의 발수성과 발수성회복 특성은 다르게 나타난다. 본 논문에서는 입도가 다른 2종의 ATH를 선택하여 실리콘 폴리머 대비 ATH의 첨가량을 달리하여 실리콘 고무의 발수성에 관련한 특성을 조사하였다. ATH의 입도 및 코로나 처리시간에 따라서 접촉각의 변화를 관찰하였으며, 코로나 처리 후 경과시간에 따른 발수성의 회복특성을 접촉각의 측정을 통하여 조사하였다.

• Journal of Energy Engineering
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• v.22 no.2
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• pp.83-89
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• 2013

Energy consumption is closely related to our lives. As Korea has four seasons, heating and cooling system is considered as essential facilities for the residential buildings. Accordingly, the government has continuously strengthened the design criteria to improve energy for new and existing building to save energy. The most important factor in the energy efficiency analysis for apartment is the heat transmission coefficient, and the value is significantly different as the heating energy amount is greatly different according to the window area ratio versus facade area ratio. Therefore, it is time to conduct continuous set-up for goal to enhance efficiency and restriction on window area ratio versus facade area ratio.

• Composites Research
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• v.23 no.2
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• pp.31-39
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• 2010

In this paper, composite panels with hat-shaped stiffeners were made using the co-curing, co-bonding and secondary bonding methods. Co-curing is a manufacturing method in which the hat part and the plate are cured simultaneously in a manner that is more cost effective than other methods. Co-bonding is a method in which the stacked prepregs are cured with other cured parts, and secondary bonding is a method in which cured parts are bonded together using an adhesive. A rubber mold was manufactured for co-curing of composite panel with hat-shaped stiffeners, and finite element analyses were done to evaluate the expanding pressure of the rubber mold consistent with the curing temperature. The manufactured panels were also evaluated using a 3-D measurement tester and an ultrasonic tester. Pull-off tests were performed to evaluate their mechanical properties.

• Research in Plant Disease
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• v.21 no.3
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• pp.235-242
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• 2015

A 2014 nationwide survey in radish fields investigated the distribution of common viruses and possible emerging viruses. Radish leaves with virus-like symptoms were collected and 108 samples assayed by RT-PCR using specific primers for Radish mosaic virus (RaMV), Cucumber mosaic virus (CMV), and Turnip mosaic virus (TuMV); 47 samples were TuMV positive, and RaMV and CMV were detected in 3 and 2 samples, respectively. No samples showed double infection of TuMV/RaMV, or RaMV/CMV, but two double infections of TuMV/CMV were detected. TuMV isolates were sorted by symptom severity, and three isolates (R007-mild; R041 and R065-severe) selected for BLAST and phylogenetic analysis, which indicated that the coat protein (CP) of these isolates (R007, R041, and R065) have approx. 98-99% homology to a previously reported TuMV isolate. RaMV CP showed approx. 99% homology to a previously reported isolate, and the CMV CP is identical to a previously reported Korean isolate (GenBank : GU327368). Three isolates of TuMV showing different pathogenicity (degree of symptom severity) will be valuable to study determinants of pathogenicity.

• 건강소식
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• v.11 no.12 s.109
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• pp.2-7
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• 1987

금방이라도 무엇인가 토해낼 것처럼 꾸무럭거리는 하늘. 그 아래로 차운 바람을 타고 소금기어린 인천바다 냄새가 코를 스친다. 미쳐 마감하지 못한 마른 나뭇잎은 이리저리 굴리고 날리고..... 4계절의 변화가 뚜렷하여 인간생활에 가장 적절한 조건을 가졌다는 생각을 떠올리면서 옷을 뚫고 스미는 찬바람을 위로한다. 그런 외피적인 계절변화와 관계없이 우리는 더 많은 변화를 겪게 되는 것 같다. 그 중에서도 특히 육신의 고통은 이 꾸무럭거리는 날씨만큼이나 차운 바람을 몰아다 주겠지...... 이런 생각에 젖으면서 건협 인천부를 방문한 기자는 친구의 권유로 건협에서 건강검사를 받았다가 간 기능이상을 발견 투병생활을 해오고 있는 육인용씨(43, 경기도 시흥군 군자면 정왕리 858번지)를 만났다.

• Research in Plant Disease
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• v.24 no.4
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• pp.285-291
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• 2018

Two isolates of Strawberry mild yellow edge virus were newly isolated in strawberry (Fragaria x ananassa) cultivar Selhyang and Kamhong from Korea. The complete nucleotide sequence of the coat protein (CP) of two Korean isolates were determined and analyzed. Sequence identity of nucleotide and amino acid between SH and KH isolates was 90.4% and 95.5%, respectively. The comparison of three Korean isolates including previously reported KNS1 with 45 SMYEV sequences from other countries deposited in GenBank database revealed an identity ranging from 81.2% to 100%. The phylogenetic analysis of CP of all SMYEV isolates showed the five subgroups (I-V), with Korean isolates being divided into two different subgroups. The isolates KH and KNS1 were included in subgroup I, whereas SH was included in subgroup IV which is new phylogenetic subgroup. Genetic diversity analysis indicated that new subgroup had greater variability and nucleotide diversity between subgroups resulted in values ranging from 0.0863 to 0.18004. This report represents the first molecular characterization of SMYEV isolates from Korea.

• Land and Housing Review
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• v.10 no.3
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• pp.23-32
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• 2019

Vacuum Insulation Panel(VIP) has the lowest thermal conductivity among present insulations. It is composed of envelope, core material and getter. Aluminum film is usually used as the envelope of VIP, and it is important component to decide the useful life of VIP. In this research, the thermophysical properties of incombustible fiber glass core VIP were investigated with the possibility of its architectural applications. The results of this research can be summarized as follows: 1) The thermal conductivity of 20mm-thick fiber glass core VIP is resulted as 0.00177W/m·K, which means that 20mm-thick VIP can meet all the reinforced insulation guideline and it can be used in any envelope of any region in Korea. 2) As a result of the test of incombustion and gas toxicity, fiber glass core VIP was suitable for incombustible material. 3) As the test result for the long term thermal conductivity, fiber glass core VIP was found out that it would keep above 10 times insulating performance than polystyrene foam and glass fiber. 4) To meet the thermal transmittance of 0.12W/㎡K, limited-combustible insulation of expanded polystyrene foam and phenolic foam should be used respectively as thick as above 280mm and 170mm, incombustible VIP can meet the same insulation level with 20mm thickness. 5) The price competitiveness of incombustible VIP to meet the thermal transmittance of 0.12W/㎡·K was about 1,500won/㎡ higher than that of phenolic foam.