• Analytical Science and Technology
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• v.13 no.5
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• pp.584-591
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• 2000

The separation of Nd from the simulated $U_3Si/Al$ spent fuel solution with sequential two-step anion exchange separation has been studied. To prepare the simulated $U_3Si/Al$ spent nuclear fuel, unirradiated $U_3Si/Al$ whose composition consists of small $U_3Si$ particle dispersed in an Al matrix with Al cladding was dissolved with a mixture of 4 M HCl and 10 M $HNO_3$ and 8 or 15 fission product elements were added to the dissolved solution. The trace amount of silica in the solutions was removed by evaporating to dryness with HF and the U was adsorbed on the first anion exchange resin. Neodymium can be purely isolated from the fission product elements with a methanol-nitric acid eluent using the second anion exchange resin. A large excess of Al didn't influence on the elution velocity of Nd, but reduced the eluted contents of Nd, Al, Eu, Gd, Sm and Sr, A large amount of Al was removed first from the column with 3 mL of loading solution (0.8 M $HNO_3$/99.8% MeOH) before Nd elution by the eluent [0.04 M $HNO_3$-99.8% MeOH(1:9)]. The recovery of Nd was more than 94%, regardless of Al contents. Taking the 9 to 13 mL fraction of eluate was effective to purely isolate Nd.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.155-164
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• 2004

This involves identifying and cloning trapped genes from cultured cells carrying the gene-trap constructs and generating cloned zebrafish using these cells for functional study. Gene-trapping studies in gene-trapped cells were carried out in initial and cloned zebrafish carrying gene-trap events were successfully produced based on the nuclear transplantation technique. Two kind of retroviral gene-trap constructs were adopted. The first one(SA/GFP-TP), constructed in my laboratory, carries a GFP reporter gene containing a splicing acceptor and an internal neo gene. The second one(Neo-TP), obtained from Dr. Hicks (Hicks et al., 1997), contains a promoter-less neo gene located in the LTR sequence of a retroviral vector. The infected cells were subjected to drug selection(neomycin treatment) because the two constructs carry the neomycin resistant gene. All those cells survived the neomycin treatment should carry the proviral insertions. For Neo-TP, Isolated DNA from the neomycin-resistant fibroblast cells infected by Neo-TP, was digested with EcoR1 restriction enzyme and transformed into bacteria after ligation. This procedure led to the isolation of seven clones carrying flanking cellular DNA with a typical retroviral integration signature sequence. These clones contained genomic DNA ranging from 1kb to 7kb and sequences of 300-600 bp were obtained from each of the rescued plasmids. Database searching showed that all of them share high homology to zebrafish sequences. For fish cloning using tagged cells, initially, nucleus donors directly selected from a mixture of cells(Neo-TP cells) were used. A total of 44 embryos(3.7%) out of 1179 transplants were reached blastula stage; 8 of these embryos(0.8%) hatched and 3(0.3%) of them survived to adulthood. One out of three lived cloned zebrafish has an amplified fragment and was labeled with 32P.

• Journal of agriculture & life science
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• v.51 no.4
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• pp.139-147
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• 2017

This study was carried out in order to reduce the amount of sawdust for recycling the generated manure from livestock farms, and to investigate the effects on the reducing usage of sawdust and quality of produced compost. To do this, a cylindrical horizontal composting device were used in the experiments and compost was analyzed for judging produce compost quality. The experiment was carried out separately under different cases of operational control conditions. The first case was produced by using sawdust and pig manure mixture(Test-1); the second case was produced by using sawdust, pig manure and the previously produced compost(Test-2). In the second case, Except for some heavy metal content, The water content and C/N ratio were found to be suitable for fertilizer process specification of the RDA(Rural Development Administration) and it was found to reduce the sawdust 1.25tons usage.

• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.25-32
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• 2014

We produced the transgenic silkworm that expressed the enhanced blue fluorescent protein (EBFP) in the cocoon of silkworms. The EBFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EBFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the $3{\times}P3$-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 300 eggs of silkworms, Baegokjam. We obtained 5 broods. The cocoon displayed blue fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and western blot analysis. Accordingly, we suggest that the EBFP fluorescence silk will enable the production of the silk-based biomaterials.

• Journal of Korea Water Resources Association
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• v.52 no.4
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• pp.265-277
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• 2019

The objective of this study was to develop and verify an effective vortex typed nonfilter nonpoint source pollution reduction device. To verify this pollution reduction device, a total of twelves scenarios (three rainfall intensities${\times}$two states${\times}$two steps) of experiments were conducted using pollutants. First, simulated inflow (rainfall intensity 2.5 mm/hr: $0.00152m^3/s$, rainfall intensity 3.395 mm/hr: $0.00206m^3/s$, rainfall intensity 6.870 mm/hr: $0.00326m^3/s$) was calculated. Second, pollutants (mixture of 25% of four particle sizes) were selected and injected. Third, pollutant removal efficiencies of this device at its initial state and operating states were measured. As a result of analysis based on rainfall intensity, the concentration of pollutants was decreased by the device at initial and operating states at all rainfall intensities. Its pollutant removal efficiency was more than 80%, the standard set by the Ministry of Environment. Its pollutant removal efficiency was gradually increased over time, reaching approximately 90%. Its pollutant removal efficiency was higher in its operating state than that in its initial state. Therefore, nonpoint source pollutants can be effectively removed by this vortex typed nonpoint source pollution reduction device developed in this study.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.4
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• pp.317-327
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• 1987

As a series of studies on the suitability as a second intermediate host of Clonorchis sinensis, artificial infection experiments were applied to Tilapia mossambica. And then, in order to elucidate the defence mechanism of the fish to Clonorchis, clonorchicidal substance in the epidermal mucus of the fish was isolated by silica gel column and thin layer chromatography and analyzed for its chemical structure by UV, IR and NMR-spectroscopy. The results obtained are summarized as follows: 1. The cercariae which attempted to contact with the fish in the water were observed under stereomicroscope. After contact, the cercariae began to separate their tail from the body after several minutes and then the number increased to $80\%$ more than 10 minutes after the encounter. But very few cercariae could actually invade the epidermis of the fish. 2. The fish were reared with Parafossarulus manchouricus which were shedding numerous cercariae of Clonorchis in the aquarium for 24 hours. Only a few cercariae could invade the epidermis but most of the invaded cercariae died out before forming their cysts. Very few number of the remaining encysted cercariae were also found to be in a state of suspended animation within 42 hours. 3. In the cases of the control fish, Pseudorasbora parva, numerous cercariae of Clonorchis were found to invade the fish through the epidermis under stereomicroscope. Then many metacercariae of Clonorchis were also found in the fish while they were kept in the aquarium. 4. A sample of the epidermal mucus of Tilapia mossambica was extracted with ethly ether 6 times repeatedly. In silica gel column chromatography, using petroleum ether: chloroform/30:70(v/v) as a first solvent and MeOH as a second solvent, the extract was fractionated into the yellow and brownish red solutions in the first solvent and the clonorchicidal brownish yellow solution in the second sovent. 5. The clonorchicidal brownish yellow solution was added to petroleum ether, and the mixture was stored for 5 days at $5^{\circ}C$ and was, then, separated into supernatant fraction and precipitate. Ten mg/ml of the supernatant fraction killed, in vitro, the excysted metacercariae in 45 minutes but the precipitate in 600 minutes. 6. In silica gel column chromatography, using acetone: benzene/10:90(v/v) as a solvent, the more clonorchicidal supernatant fraction was fractionated into the first fraction with Rf. 0.2966 and the second fraction with Rf. 0.072. In vitro, 10mg/ml of the first fraction killed the excysted metacercariae in 28 minutes, the second fraction in 80 minutes and the first fraction was, therefore, determined to be a final clonorchicidal substance. 7. By this purification procedures, the most clonorchicidal substance from the epidermal mucus of Tilapia mossambica was purified 71-folds with $0.2075\%$ yield. Infra red, nuclear magnetic resonance and ultraviolet spectrometric analysis of the purified substance revealed that the substance is linoleic acid. According to the results of the present studies it seemed that this species can not serve as a proper intermediate host of Clonorchis sinensis, and that defence mechanism to the fluke seems to be correlated with linoleic acid in the epidermal mucus of this species.

• Journal of the Korea institute for structural maintenance and inspection
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• v.18 no.2
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• pp.117-125
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• 2014

This paper examines the fundamental properties of alkali-activated slag cement (AASC) activated by sodium hydroxide (NaOH). The water to binder (W/B) ratio was 0.4 and 0.5. And concentration of activator were 2M and 4M. Five mix design of each W/B ratios was considered. The N0 mixture was KS L 5109 method and N1~N4 were varied in different mixing time, mix step and entering points of fine aggregate. Test results clearly showed that the flow value, strength and drying shrinkage development of AASC were significantly dependent on the entering point of fine aggregate. The flow value tended to decreases with delaying entering point of fine aggregate. The compressive strength and flexural strength increases with delaying entering point. Moreover, the XRD analysis confirmed that there were sustain these results. The drying shrinkage increases with delaying entering point of fine aggregate. Futhermore, a modified mixing method incorporating all hereby experimentally derived parameters, is proposed to improvement the physical properties of AASC.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.673-680
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• 2017

sump pump is a system that draws in water that is stored in a dam or reservoir. They are used to pump large amounts of water for cooling systems in large power plants, such as thermal and nuclear plants. However, if the flow and sump pump ratio are small, the flow rate increases around the inlet port. This causes a turbulent vortex or swirl flows. The turbulent flow reduces the performance and can cause failure. Various methods have been devised to solve the problem, but a correct solution has not been found for low water level. The most efficient solution is to install an anti-vortex device (AVD) or increase the length of the sump inlet, which makes the flow uniform. This paper presents a computational fluid dynamics (CFD) analysis of the flow characteristics in a sump pump for different sump inlet lengths and AVD types. Modeling was performed in three stages based on the pump intake, sump, and pump. For accurate analysis, the grid was made denser in the intake part, and the grid for the sump pump and AVD were also dense. 1.2-1.5 million grid elements were generated using ANSYS ICEM-CFD 14.5 with a mixture of tetra and prism elements. The analysis was done using the SST turbulence model of ANSYS CFX14.5, a commercial CFD program. The conditions were as follows: H.W.L 6.0 m, L.W.L 3.5, Qmax 4.000 kg/s, Qavg 3.500 kg/s Qmin 2.500 kg/s. The results of analysis by the vertex angle and velocity distribution are as follows. A sump pump with an Ext E-type AVD was accepted at a high water level. However, further studies are needed for a low water level using the Ext E-type AVD as a base.

• Journal of Animal Environmental Science
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• v.15 no.3
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• pp.271-280
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• 2009

The best way to treat livestock manure is to recycle as much as possible. The composting of livestock manure is a safe and economical treatment process. This study was carried out to investigate decomposition effect of pig manure by adding fermented compost. The fermented compost was added in pig manure mixed with sawdust as an inoculator, and the mixture was fed to composting reactor. Supplemental levels of fermented compost on the pig manure mixed with sawdust were regulated at 5, 10, 15 and 20% (V/V) respectively. The results were as follows ; 1. In all cases, PH range was between 7.6~9.05 during composting period. 2. The highest temperature and the long duration of thermophilic stage were observed in control treatment. 3. The number of microorganism reached at maximum on day 4, which recorded the highest temperature 4. Compost pile mixed with 10% of inoculator (fermented compost) showed the highest C/N ratio reduction.

• Journal of Chest Surgery
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• v.35 no.3
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• pp.171-176
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• 2002

Background: Blood-foreign interaction cause activation of coagulation and inflammatory process that may lead to multiorgan dysfunction and determine the surgical outcomes. Of the methods for assessing the biocompatibility, the platelet adhesion study is considered as the most valuable evaluation step in blood-foreign interaction. As the most studies have used in-vitro or ex-vivo conditions, we have developed a technique of quantification for platelet adhesion on the blood contact surface by using in-vivo injection of radioactive platelets. Material and Method: A coupled bypass circuit was designed to connect the proximal and descending thoracic aorta in 6 piglets(20∼25 Kg). One side of the circuit tube was consisted of a heparin coated PVC tube(10mm in ID, n=6, Experimental group), and the other, a non-heparin coated PVC tube(10mm in ID, n=6, Control group). After cannulation, the blood was circulated through the circuit for 2 hours. Platelet concentrate was prepared from homologous pig blood 24 hours before the experiment. The platelet concentrate was incubated with Tc-99m-HMPAO for 30 min and then centrifuged for 10 min. The supernatant was discarded and the radio-labeling efficacy was measured. The radio-labeled platelet concentrate was mixed with the autologous plasma to make the volume 5 ml, and the mixture was injected intravenously into the experimental animal. After 2 hour circulation, 5 pieces of the specimen(10mm in length each) were obtained from each PVC tube. The radioisotopes were counted with a gamma counter(Cobra ll, Packard, USA), and the ratio of radioisotope count was compared between the control and experimental group. Result: The radioisotope count number was 537.3221.1 Ci/min in the control group and 311.1 184.5 Ci/min in the experimental group(p=0.0104). The ratio between the groups was 1 to 0.58 (p=0.004). Conclusion: In vivo quantification using technetium-99m-HMPAO labeled platelets is simple and reproducible in evaluating platelet adhesion on a foreign surface. We suggest this technique to be a useful tool for blood compatibility test.