• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.193-198
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• 2007

The present study was examined the expression patterns of cdx2 gone, n lineage marker, in the mouse and bovine developmental stage embryos and whether one blastomere of two- and/or four-cell bovine embryos develop to specific lineage (ICM or TE) of blastocyst by injection of Texas red conjugated dextran as a lineage tracer. It was also investigated the allocation of ICM and n cells in bovine blastocysts derived from one blastomere of two-and/or four-cell stage embryos. Firstly, it was observed that expression of cdx2 appeared symmetric and asymmetric distribution at the two-cell stage mouse embryos. from four-cell to morula stage mouse embryos, the expression of cdx2 gene was observed in almost all blastomeres. In case of bovine embryos, localization of cdx2 was similar to pattern of mouse embryos. The Dextran-labeled blastomere of two- and/or four-cell embryos contributed to both ICM and TE cells in bovine blastocysts. And also, it was confirmed that a single blastomere derived from two-cell stage bovine embryos could develop to the normal blastocyst with both ICM and TE cells. These results show that two-and/or four-cell stage is not the specific stage to determine the cell rate for ICM and TE, and which is not correlated with the expression of cdx2 gene.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.187-194
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• 2011

The objective of this study was to determine the effects of light intensity and electrical conductivity (EC) of nutrient solution on the growth and nutrient uptake of potted kalanchoe plants (Kalanchoe blossfeldiana 'Marlene') with growth stage in ebb and flow subirrigation systems. The plants were grown at four ECs of 0.5, 1.0, 1.5, and 2.0 $dS{\cdot}m^{-1}$ for seedling stage and four ECs of 1.0, 1.5, 2.0, and 3.0 $dS{\cdot}m^{-1}$ for short day stage under three daily photosynthetic photon flux (PPF) of 6.5, 10.3, 18.2 $mol{\cdot}m^{-2}{\cdot}d^{-1}$. At seedling stage, plant height was the longest under the lowest light intensity, and particularly dry weights and leaf areas were the highest at PPF 10.3 $mol{\cdot}m^{-2}{\cdot}d^{-1}$. Dry weights and leaf areas were the highest at EC 1.5 $dS{\cdot}m^{-1}$ regardless of light intensity. At short day exposure, plant height was the longest under the lowest light intensity. Dry weights, leaf areas, and number of pedicels of the plants significantly increased as light intensity increased. Under all light intensity conditions, dry weights, leaf areas, and number of pedicles increased until EC becomes to 1.0 - 2.0 $dS{\cdot}m^{-1}$. And after reached the highest at EC 2.0 $dS{\cdot}m^{-1}$, they decreased at EC 3.0 $dS{\cdot}m^{-1}$. By comparing the ion uptakes at EC 1.5 $dS{\cdot}m^{-1}$ of seedling stage and EC 2.0 $dS{\cdot}m^{-1}$ of short day stage in which the plants grew better, we confirmed that ion balance of nutrient solution among $NO_3{^-}$-N, $H_2PO_4{^-}$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$ were significantly changed at short day stage compared to seedling stage. For better growth of the plants, both ion balance and EC of nutrient solution should be considered under different light intensities at short day stage while control of EC is enough at seedling stage.

• Journal of the Korean Chemical Society
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• v.36 no.4
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• pp.546-551
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• 1992

Reaction mechanism on the solvent extraction of nickel(Ⅱ) with N-benzylisonitrosoacetylacetone imine(HIAANB) was studied spectrophotometrically. Absorbance was measured by changing the ligand HIAANB concentration in the chloroform organic phase and the pH values in the agueous solution phase. From the absorbance data, the reaction rate was found to be the first order for HIAANB concentration and the inverse first one for [$H^+$]. The rate determining step of the extraction reaction and the rate equation are as follows; $Ni^{2+}$＋HIAANB ${\to}$ Ni-IAANB$^+$＋$H^+$ -d[Ni$^{2+}$] / dt = K'[Ni$^{2+}$][HIAANB]$_0$ / [H$^+$] Calibration curve for the spectrophotometric determination of nickel(Ⅱ) ion in the aqueous solution was linear below the concentration of 1.17 ppm at the optimum experimental condition. And the ligand-to-metal ratio, the relationship between extractability and pH of the aqueous phase, and the effect of diverse ion on the determination of nickel(Ⅱ) ion were examined.

• Korean journal of applied entomology
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• v.35 no.3
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• pp.205-208
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• 1996

Psylla pyricola population from Suwon($37^{\circ}$16' N) begins to enter diapause by exposure to a photoperiod of 14hr light. Over 93% of adults were induced to enter diapause when exposed to 13hr loght period, and at 18 and $25^{\circ}C$, the critical photoperiod was not influenced. When the photoperiod was switched during the nymphal stage from 16L to 10L, no morph change was occurred in the 4th and 5th instars. Average number of eggs laid per female was 486.2 in winter form adult and 387.2 in summer form adults, and average oviposition periods were 34 and 24 days, respectively.

• The Korean Journal of Zoology
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• v.4 no.2
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• pp.1-6
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• 1961

바지락(Tapes philippinarum) 직모연동능에 미치는 몇가지 중요한 환경 요인의 영향에 관하여 생리학적 방법으로 조사하였다. 1. 산화환워전위 측정결과, 치매일수록 성매에 비하여 무기상태에 대한 저항성이 낮았다. 2. 치매의 직모연동능은 17$\pm$1$^{\circ}C$에서 산소장력 저하상태로 \ulcorner긴 20분후, 성매의 약 1/2 이하로 감소되었따. 3. 한편 수온 13$\pm$1$^{\circ}C$dpt는 직모운동능이 같은 산소장력의 저하에 의하여서도 성매 치매 모두가 비슷한 경향으로 급격히 억제되었다. 4. 수온 1$0^{\circ}C$에서 20 $^{\circ}C$로 옮겼을 때 성매와 치매의 직모연동능은 수온으로 인하여 거의 평행하게 최고치 약 2305까지 촉진되었다. 5. 특히 치매에 있어서는 부유토분자의 낮은 혼촉도에 의하여 직모연동능이 감소되었다. 6 바지락은 광감성물질임을 밝혔다. 7 . 이들 결과로서 치매의 높은 치사율은 해저서식지의 불리한 환경요인 특히 중량요인에 좌우되는 것임이 확실하다. 치사율을 좌우하는 가장 중요한 요인은 용존산소의 결지, 저온 그리고 심한 부유토의 온촉도이며 반하여 약간의 염도변동은 그리 치명적인 것이 아니다.

• The Korean Journal of Mycology
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• v.17 no.3
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• pp.161-168
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• 1989

Mitochondria in L. edodes were separated and purified by stepped sucrose density gradient centrifugation. In our previous work, we have found that the activation wavelengths of the mitochondrial ATPase and ATP synthase were 680 nm and 470 nm within the range of 400-700 nm, respectively. The activities of the above enzymes with wavelengths of 300-400 nm region were investigated. The mitochondrial ATPase and ATP synthase were stimulated at 380 nm and 330 nm, respectively, for 30 min illumination compared with dark control group. They, however, were inhibited at 330 nm and 350 nm, respectively. The presence of FAD resulted in inhibition of the activity of the ATPase and stimulation of the activity of the ATP synthase by the activation and inhibition wavelengths. However, the activities of these enzymes were not changed by NADH for the above wavelengths. In the spectral properties, the oxidation of $FADH_2$ into FAD occurs in the presence of the enzymes for illumination of the activation and inhibition wavelengths. Therefore, we can predict that the mitochondrial ATPase and ATP synthase may function as oxidant in the redox reaction by the light illumination and that the light-induced pigment of the mitochondrial ATP synthase should be an oxidized form of a flavoprotein.

• Journal of Environmental Science International
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• v.5 no.5
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• pp.635-642
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• 1996

Rice plants, cv. Koshihikari, were subjected to the biologically effective ultraviolet-B(UV-BBE) radiation {daily dose : 0.0 (control) and 11.5 (enhanced UV-B) KJ m-2} to investigate the effect of enhanced UV-B radiation on lipid peroxidation and to determine whether carotenoids and polyamines are involved in protection mechanism against enhanced UV-B radiation. Enhanced UV-B radiation significantly depressed plant dW weight. Malondialdehyde (MDA) content in rice leaves was increased by about 30% after 6 days of UV-B irradiation. Total carotenoid contents tended to slightly decrease with the UV-B irradiation, even though there was no significance. In rice leaves, 3 major polyamines, putrescine, spermidine and spermine are observed. All of the polyamine contents were increased with UV-B irradiation. The results suggest that enhanced UV- B radiation caused oxidative stress on lipids and that polyamines may serve as a biochemiral protectant against increased UV-B radiation in rice plants.

• Journal of the Korean Chemical Society
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• v.37 no.10
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• pp.888-894
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• 1993

The electrochemical behaviors of copper-1-(2-pyridylazo)-2-naphthol(Cu-PAN) complex in acetonitrile (AN) solution have been investigated by the use of cyclic voltammetry, DC-polarography, controlled potential coulometry and UV-Vis spectroscopy. Cu-PAN complex in acetonitrile exhibit three reduction waves at -1.27 V, -1.64 V and -2.08 V vs. Ag/AgNO$_3$(AN). The numbers of electron involved in each reduction step was calculated with controlled potential coulometry, and reduction product was identified with UV-Vis spectrum. As the result, we proposed the reduction mechanism of the Cu-PAN complex in acetonitrile.

• KSBB Journal
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• v.19 no.4
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• pp.257-262
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• 2004

Recently, there is a growing interest in microalgae and the use of microalgae focused on the production of various high value metabolite used in food, pharmaceuticals and cosmetics. The key limiting factor in high density algal cultivation is the light and algal growth is defined by light intensity and light penetration depth into the culture medium. The effect of light with various light paths, S/V ratios, light intensities, and 50% duty cycle on the growth of microalgae was examined to enhance microalgal biomass productivity and photosynthetic efficiency. We confirmed that the utilization of efficient light energy was obtained from 4 cm of diameter, 57.6% of S/V ratio, 62 ${\mu}$mol/㎡/s of light intensity.

• Journal of Astronomy and Space Sciences
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• v.21 no.4
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• pp.417-428
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• 2004

We have investigated the optical property of the KAO(Korea Astronomy Observatory) wide field telescope (named NEOPAT-3; Near Earth Object and Satellite Patrol-3) and aligned optical system. The NEOPAT-3 is restricted to V,R,I-filters because of the refractive property of the correcting lens system. Because of the fast focal ratio, the optical performance of the NEOPAT-3 is very sensitive to its alignment factors of the optical system. To make the spot radius smaller than $8{\mu}m$ in rms over 2degree${\times}2$degree field, the optical system must satisfy the following conditions: 1) The tilt error between detector plane and focal plane should be less than 0.05degree. 2) The decenter error between the primary mirror and the correcting lens system should be less than 1mm. 3) The distance error between the primary mirror and the correcting lens system should be less than 2.3mm. In order to align the optical system accurately, we measured the aberrations of the telescope quantitatively by means of curvature sensing technique. NEOPAT-3 is installed temporary on the roof of the TRAO(Taeduk Radio Astronomy Observatory) main building to normalize system performance and to develop automatic observation.