• Journal of Dental Rehabilitation and Applied Science
• /
• v.31 no.2
• /
• pp.75-85
• /
• 2015

Purpose: The purpose of this study is to evaluate which FRC-posts were more distinguishable from core. Materials and Methods: Nine extracted single-rooted mandibular premolars with similar lengths (${\pm}0.5 mm$) and widths (${\pm}0.5 mm$) were endodontically treated and obturated. One specimen which the three roots were embedded in a cold mounting resin was made. 3 specimens were made by this method. Post spaces were prepared for the following post system: FRC $Postec^{(R)}$ Plus, MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$, $Snowpost^{(R)}$. After three different posts were placed in the roots of a specimen, each three specimens received a direct core build-up: $Luxacore^{(R)}$ Dual A3, $Luxacore^{(R)}$ Dual blue, $Filtek^{TM}$ Z350 A1E. Digital images were taken of the post and core with and without air-blowing. We asked to fifty dentists and fifty dental college students which post was more clearly discriminated from the core. Results: In surveys, when core was $Luxacore^{(R)}$ Dual blue, among three types of posts people more easily discriminated the $Snowpost^{(R)}$ from core. When core was $Luxacore^{(R)}$ Dual A3, among three types of posts people similarly more easily discriminated $Snowpost^{(R)}$ from core. When core was $Filtek^{TM}$ Z350 A1E, among three types of posts people more easily discriminated distinguished MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$ post from core. People more easily distinguished MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$ post from core when temperature was lowered by air-blowing. Conclusion: Ability to discriminate between FRC-post and core is different according to color contrast. MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$ posts are more discriminable when temperature is lowered by air-blowing.

• Korean Journal of Microbiology
• /
• v.42 no.3
• /
• pp.185-194
• /
• 2006

The aim of this study was to identify the bacteria isolated from endodontic lesions by cell culture and to determine the antimicrobial susceptibility of them against 8 antibiotics. The necrotic pulpal tissues were collected from 27 infected root canals, which were diagnosed as endodontic infection. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing $500{\mu}l\;of\;1{\times}PBS$. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a $37^{\circ}C$ anaerobic chamber for 2 to 5 days. The bacteria grown on the agar plates were identified by comparison of 16S rRNA gene (rDNA) sequencing method at the species level. To test the sensitivity of the bacteria isolated from the infected root canals against 8 antibiotics, minimum inhibitory concentrations (MIC) were determined using broth dilution assay. The data showed that 101 bacterial strains were isolated and were identified. Streptococcus spp. (29.7%) and Actinomyces spp. (21.8%) were predominantly isolated. The 9 strains were excluded in antimicrobial susceptibility test because they were lost during the experiment or were not grown in broth culture. The percentage of bacteria susceptible for each antibiotic in this study was clindamycin, 87.0% (80 of 92); tetracycline, 75.0% (69 of 92); cefuroxime axetil, 75.0% (69 of 92); amoxicillin + clavulanic acid (5:1), 71.7% (66 of 92); penicillin G, 66.3% (61 of 92); erythromycin, 66.3% (61 of 92); amoxicillin, 44.6% (41 of 92); and ciprofloxacin, 31.5% (29 of 92). The susceptibility pattern of 8 antibiotics was dependent on the host of the bacteria strains rather than the kinds of bacterial species. These results indicate that antibiotic susceptibility test should be performed when antibiotics are needed for the treatment of infected root canals.

• Journal of Nutrition and Health
• /
• v.57 no.1
• /
• pp.53-64
• /
• 2024

Purpose: Mitochondria play a crucial role in preserving skeletal muscle mass, and damage to mitochondria leads to muscle mass loss. This study investigated the effects of oxypeucedanin hydrate, a furanocoumarin isolated from Angelica dahurica radix, on myogenesis and mitochondrial function in vitro and in zebrafish models. Methods: C2C12 myotubes cultured in media containing 0.1, 1, 10, or 100 ng/mL oxypeucedanin hydrate were immunostained with myosin heavy chain (MHC), and then multinucleated MHC-positive cells were counted. The expressions of markers related to muscle differentiation, muscle protein degradation, and mitochondrial function were determined by quantitative reverse transcription polymerase chain reaction. To investigate the effects of oxypeucedanin hydrate on mitochondrial dysfunction, Tg(Xla.Eef1a1:mito-EGFP) zebrafish embryos were treated with 5-fluorouracil, leucovorin, and irinotecan (FOLFIRI) with or without oxypeucedanin hydrate and analyzed for mito-EGFP intensity and mitochondrial length. Results: Oxypeucedanin hydrate significantly increased MHC-positive multinucleated myotubes (≥ 3 nuclei) and increased the expression of the myogenic marker myosin heavy chain 4. However, it decreased the expressions of muscle-specific RING finger protein 1 and muscle atrophy f-box (markers of muscle protein degradation). Furthermore, oxypeucedanin hydrate enhanced the expressions of markers of mitochondrial biogenesis (peroxisome proliferator-activated receptor-gamma coactivator 1 alpha, transcription factor a mitochondrial, succinate dehydrogenase complex flavoprotein subunit A, and cytochrome c oxidase subunit 1) and mitochondrial fusion (optic atrophy 1). However, it reduced the expression of dynamin-related protein 1 (a mitochondrial fission regulator). Consistently, oxypeucedanin hydrate reduced FOLFIRI-induced mitochondrial dysfunction in the skeletal muscles of zebrafish embryos. Conclusion: The study indicates that oxypeucedanin hydrate promotes myogenesis by improving mitochondrial function, and thus, suggests oxypeucedanin hydrate has potential use as a nutritional supplement that improves muscle mass and function.

• Restorative Dentistry and Endodontics
• /
• v.23 no.1
• /
• pp.54-67
• /
• 1998

The purposes of this study were to evaluate the efficiency of dentin cutting and root-end cavity preparation, and to determine the incidence of tooth crack when root-end retrograde cavity preparation was done with. ultrasonic diamond instruments. To evaluate the efficiency of dentin cutting, ultrasonic diamond and stainless steel instruments were applied to 20 exposed bovine dentin surfaces perpendicularly or parallely at the low, and medium power settings for 1 minute ($Miniendo^{TM}$, EIE, CA, U.S.A.). The resultant cavity depth was measured. To evaluate the efficiency of cavity preparation and to investigate the incidence of tooth crack, 165 mesiobuccal, distobuccal and palatal root-ends of extracted human maxillary first molars were resected by 3 mm perpendicularly to the long axis of tooth using a slow speed diamond saw after root canal preparation and filling. Retrocavities were prepared using a ultrasonic diamond instrument or a stainless steel one of the low- or medium power settings of 2 or 6. Time consumed and the number of strokes used for the cavity preparation were measured and the incidence of tooth cracks was evaluated under a stereomicroscope. The results were as follows: Both at the low and medium power settings, and both with perpendicularly- and parallely applied tips to dentin, diamond instruments showed higher dentin cutting efficiency than stainless steel ones did (p<0.01). When tips were applied to dentin perpendicularly, both diamond instrument and stainless steel one showed higher cutting efficiency with medium power setting than with low power one (p<0.01). Both at the low- and medium power settings, both diamond instrument and stainless steel one showed higher cutting efficiency when tips were applied perpendicularly to dentin surface than applied parallely (p<0.01). At the medium power setting, the number of stroke and time consumed were less with diamond instrument than with stainless steel one (p<0.05) for the retrograde cavity preparation. At the low power setting, diamond instrument induced less tooth cracks than stainless steel one did (p<0.01).

• Korean Journal of Heritage: History & Science
• /
• v.51 no.1
• /
• pp.80-99
• /
• 2018

In premodern society, the central spaces in local counties were known as Eupchi (邑治), in which Gwan-a (官衙). The Eupchi located in seaside counties were in Eupseong, whereas the Eupchi of inland counties only included a Gwan-a, and did not have a fortress. Yanggeun-gun (楊根郡) and Jipyeonghyeon (砥平縣), which currently form Yangpyeong-gun (楊平郡), were counties where only Gwan-a existed. At present, there are few traces of the Gwan-a in Yanggeun and Jipyeong. In addition, it is impossible to exactly verify the history, scale and location of these two Gwan-as. Therefore, this study attempts to make a rough estimation of the histories, scales and sites of these Gwan-a by analyzing the relevant literature and ancient maps that are scattered according to the principles of spatial organization of local Gwan-a. The Yanggeun and Jipyeong Gwan-a appear to have been built in the early Joseon period. There are three reasons for this assertion: First, in Yanggeun and Jipyeong, Suryeong (守令) were first officially appointed after the early Joseon period. Once Suryoeng were appointed to such counties, the Gwan-a in which they would serve could be established. Second, the Dongheon (東軒) in Yanggengun and the Gaeksa (客舍) in Jipyeonghyeon were rebuilt in 1703 (29th year of the reign of King Sukjong) and 1465 (11th year of the reign of King Sejo), respectively; thus; the Gwan-a must have existed since the early Joseon period.. Third, in Yanggeungun, the Hyanggyo (鄕校) had existed since the early Joseon period, as the Joseon government necessarily established the Hyanggyo in areas where Suryeong were established. The facilities of local Gwan-a in the Joseon period were largely divided into gonghae (公?), educational institutes, and alters. The gonghae was a facility in which the Suryeong and Gwanwon (官員) managed administrative, judicial and military affairs, and consisted of the A-sa (衙舍), Hyeongcheong (鄕廳), Jackcheong (作廳), Gaeksa (客舍), Gungwancheon (軍官廳), and so on. The education institute was called a Hyeonggyo, while alters included the Sajikdan (社稷壇), Seonghwansa (城隍祠) and Yeodan (?壇). Both the Yeongeun and Jipyeong Gwan-a also consisted of such facilities as A-sa, Hyangcheong, Jakcheong, Gaeksa, Gungwancheong, Hyeonggyo, Changgo, Sajikdan, Seonghwangsa, Yeodan, Saryeongcheong, Jiincheong and Gwannocheong. The present sites for both Gwan-as cannot be identified with any certitude. However, they can be roughly identified by connecting the related literatures, oral statements and relics with the principles of spatial organization of local Gwan-a in the Joseon period. ${\bullet}$ Address of the Yanggeun A-sa (Dongheon): the whole of # 15, Gwanmun 1-gil, Yangpyeong-eup (Yanggeun-ri 151) in front of Yanggeun Junior High School at present. ${\bullet}$ Address of Yanggeun Gaeksa: around the Yangpyeong police station at present. ${\bullet}$ Address of Jipyeong A-sa(Dongheon): the whole of Jipyeong-ri 248-250, Jipyeong-myeon at present. ${\bullet}$ Address of Jipyeong Gaeksa: the whole of Jipyeong-ri 238, Jipyeong-myeon at present.