• Bulletin of the Korean Chemical Society
• /
• v.30 no.10
• /
• pp.2341-2344
• /
• 2009

Metallacyclodimers, [Ag(OTf)($L1)]_2$ and [Ag($L2)]_2(OTf)_2$ (L1 = 1,3-dibromo-2,2-bis[(isonicotinoyloxy)methyl] propane; L2 = 2,5-dimethyl-2,5-bis(isonicotinoyloxy)hexane) were constructed and characterized. The crystal structure of [Ag(OTf)($L1)]_2$ reveals a 32-membered cyclodimer, whereas that of [Ag($L2)]_2(OTf)_2$ shows a linked 34-membered cyclodimer chain via intercyclic argentophilic (Ag…Ag) interactions. [Ag(OTf)($(L1)]_2$ affords “intramolecular $\pi-\pi$ interaction cyclodimer” whereas [Ag($L2)]_2(OTf)_2$ produces a racemic mixture of “twisted cyclodimer”. Ring-conformation of the cyclodimers was affected via the competition of electrostatic interaction and argentophilic interaction.

• Journal of Plant Biotechnology
• /
• v.7 no.4
• /
• pp.267-272
• /
• 2005

Common bean (Phaseolus vulgaris L.) plants were regenerated via organogenesis from mature embryonic axes, cultured on MS medium supplemented with ildole-3-ecetic acid (IAA) and thidiazuron (TDZ) for one week in the dark. Embryonic axillary regions were excised, longitudinally cut to split the both sides, and cultured for two weeks on MS medium supplemented with IAA and TDZ. The combination 0.5 mg $l^{-1}$ TDZ/0.5 mg $l^{-1}$ IAA presented the higher efficiency in shoot regeneration and the combination 0.5 mg $l^{-1}$ TDZ/0.25 mg $l^{-1}$ IAA presented the higher efficiency in conversion of shoots to plants. Regenerating explants were transferred to MS medium containing 1 mg $l^{-1}$ BAP for shoot development. All elongated shoots were rooted in vitro, presented normal phenotype and produced viable seeds. Histological analysis confirmed the mode of regeneration as de novo shoot organogenesis.

• Journal of Korean Society of Forest Science
• /
• v.79 no.2
• /
• pp.109-114
• /
• 1990

The axillary buds of 15-year-old Tilia amurensis were cultured on Saito and Ide (IS), Murashige and Skoog (MS) media and woody plant medium (WPM) to establish an effective micropropagation method. Five levels of 6-benzylaminopurine (BAP) were tested. On IS medium and WPM addition of 1.0/l BAP enhanced shoot development and shoot elongation, whereas addition of 0.5/l BAP was effective on MS medium. A better results were obtained from WPM with 1.0/l BAP and MS with 0.1/l BAP. Developed shoots were subcultured on each basal media but with 0.2/l BAP, Multiple shoots were almost doubled in a month. Root formation could be enhanced at higher concentration of indole-3-butyric acid (IBA). Better rooting rate (83.3%) was achieved on a half-strength MS medium with 3.0 /l IBA. Regenerated plantlets were successfully transferred to soil. To investigate the clonal variation in shoot development and shoot elongation by axillary bud culturing, seven plus tree clones were tested, Clonal variation in tissue culturability among plus trees was recognized by the Duncan's multiple range test at the 5% level. Kang Won No. 12 showed the best response on WPM with 1.0/l BAP.

• The Korean Journal of Mycology
• /
• v.25 no.4 s.83
• /
• pp.257-267
• /
• 1997

Fruit bodies similar to the Phellinus sp. residing on the mulberry were collected at Yang-yang in Kang-won-do province and one strain of Phellinus sp. was isolated from the fruit bodies. For mass production of the isolated mycelia in a submerged culture, the culture conditions, medium composition, and the effect of various culture systems on the mycelial growth, were investigated. The morphological characteristics of the fruit body were as follows: covered with blackish to black and rough, lower surface with yellowish-brown to dull-brown and smooth, 5-7 cm thick and hard woody. Also, the pure cultured mycelia showed yellowish-brown color, capability of purplish-brown pigment production on the PDA plate media, no-formation of clamp-connection, much binding branch, and enzyme activities such as laccase, tyrosinase and peroxidase. Therefore, pure cultured strain was identified to be Phellinus sp. In the flask culture, the optimum culture conditions for the mycelial production were obtained after cultivation of 8 days at inoculum level of 5%(v/v), media volume of 70 mL, 150 rpm, initial pH 6, and temperature of $30^{\circ}C$. Optimum medium composition from the response surface analysis were determined to be glucose 12.12 g/L, sucrose 12.12 g/L, yeast extract 11.15 g/L, malt extract 11.15 g/L, $KH_2PO_4$ 0.855 g/L and $CaCl_2$ 0.855 g/L. The production of the mycelia after 4 and 8 days of cultivation was 1.95 and 9.89 g/L, respectively. The maximum specific growth rate and productivity were $0.020\;hr^{-1}$ and 1.25 g/L/day, respectively. Among the three different culture systems for the growth of mycelia, the maximum mycelial dry weight of 7.5 g/L was obtained after cultivation of 4 days in the air-lift fermentor under aeration rate of 2.5 vvm. The maximum specific growth rate and productivity were $0.033\;hr^{-1}$ and 1.9 g/L/day, respectively, which were about 1.7 and 4.2 times higher than those of flask culture.

• Journal of Plant Biology
• /
• v.39 no.3
• /
• pp.167-172
• /
• 1996

Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

• Mass Spectrometry Letters
• /
• v.14 no.3
• /
• pp.110-115
• /
• 2023

The reaction of (COD)PdCl₂ with new C₃-symmetric tridentate L (COD = 1,5-cyclooctadien; L = 1,3,5-tris(picolinoyloxyethyl)cyanurate) in a mixture of acetone and dichloromethane produces single crystals consisting of unprecedented monometallacyclic [PdCl₂(L)]. This cyclic compound arises from trans-chelation of two of three donating pyridyl groups of L, while the third pyridyl group remains uncoordinated. Electrospray ionization mass spectrometry (ESI-MS) data on L exhibited the major peak corresponding to [C₂₇H₂₄N₆O₉ + H⁺]⁺. Fast atom bombardment mass spectrometry (FABMS) data on [PdCl₂(L)], however, showed the mass peak corresponding to the L instead of the present palladium(II) compound species, due to the insolubility and dissociation in solution. The physicochemical properties of the present palladium(II) compound were fully characterized by means of infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy, thermal analysis, single-crystal X-ray diffraction (SC-XRD) measurement.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2012.05a
• /
• pp.906-906
• /
• 2012

밭에서 발생하는 비점오염물질은 강우량, 강우강도, 경사도, 토양, 시비량 등에 영향을 받는다. 따라서 효율적인 비점오염물질의 관리를 위해서는 영향인자에 대한 자세한 분석이 선행되어야 한다. 이에 본 연구에서는 강원도 평창군 대관령면의 경사도가 다른 고랭지 밭 두 지점을 선정하여 비점오염물질의 유출특성을 파악하고, 향후 비점오염원의 효율적인 관리를 위한 기초자료를 제공하고자 하였다. 연구기간은 2011년 4월부터 11월까지 유출이 발생한 8회의 강우사상에 대해 모니터링을 수행하였다. 연구결과 경사도 4.9% 밭에서의 유출율은 0.05~0.48이었으며, 16.8% 경사에서는 0.31~0.57로 나타났다. 유량과 채취된 수질 시료를 이용해 EMC를 산정한 결과 경사도 4.9%인 밭에서는 SS 762.1~2422.7 mg/L $BOD_5$ 4.5~14.9 mg/L, $COD_{Cr}$ 16.1~62.0 mg/L, $COD_{Mn}$ 7.5~43.2 mg/L, TN 9.602~21.021 mg/L, TP 2.544~6.763 mg/L의 범위로 나타났다. 반면 16.8%의 경사밭에서는 SS 116.0~2015.5 mg/L $BOD_5$ 6.0~9.5 mg/L, $COD_{Cr}$ 21.9~75.4 mg/L, $COD_{Mn}$ 4.3~33.1 mg/L, TN 10.937~46.295mg/L, TP 2.611~11.197 mg/L의 범위로 나타났다. 두 지점의 유출률과 EMC를 비교한 결과, 경사가 증가함에 따라 유출율이 증가하는 것으로 나타났으며, SS를 제외한 모든 수질항목의 EMC가 증가한 것으로 나타났다. SS의 경우 경사가 큰 고랭지 밭에서 낮은 값을 보였는데, 이는 16.8%의 경사밭에서 등고선 방향으로 경운을 하여 흙 입자의 유실이 적었기 때문인 것으로 판단된다. 본 연구의 결과와 같이 밭에서 발생하는 비점오염물질의 유출특성은 강우조건(강우강도, 선행무강우일수), 시비량, 토양, 지표의 피복상태 등에 따라 다르기 때문에 장기적인 모니터링을 통해 기초자료를 축적하는 것이 중요하다고 판단된다.

• Journal of Korean Society of Environmental Engineers
• /
• v.22 no.2
• /
• pp.355-362
• /
• 2000

The objective of this paper is to estimate the pollutant load based on event mear concentrations(EMC) in industrial complex. Eight sub-basins in the Chongju industrial complex were selected for sampling and study with different characteristics during the period from June 1997 to August 1998. During the storm events, measured EMC ranges of $BOD_5$, COD, SS, TKN, TP, HEM, $NO_3-N$, $PO_4-P$, Cr, Pb, Cu and Fe in the industrial complex watersheds were 11~176mg/L, 40~502mg/L, 23~633mg/L, 104~20.9mg/L, 0.22~7.51mg/L, 12.7~548.7mg/L, 0.06~2.66mg/L, 0.12~3.39mg/L, 0.01~0.50mg/L. 0.02~0.42mg/L, 0.01~0.15mg/L and 1.29~11.51mg/L respectively. And the calculated annual average pollutant unit loadings of $BOD_5$ COD, SS, TKN, $NO_3-N$, $PO_4-P$, TP, HEM, Cr, Pb, Cu and Fe were 374.4kg/ha/yr 924.1kg/ha/yr, 983.6kg/ha/yr, 48.8kg/ha/yr, 8.1kg/ha/yr, 9.7kg/ha/yr, 17.8kg/ha/yr 943.0kg/ha/yr, 0.7kg/ha/yr, 0.9kg/ha/yr, 0.3kg/ha/yr and 28.9kg/ha/yr, respectively.

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.1
• /
• pp.78-85
• /
• 1996

The effects of growth regulators, sucrose and gelling agents were investigated to increase the efficiency of the callus growth and plant regenerarion in tissue culture of Angelica koreana Max. The fresh weight and dry weight of subcultured callus was highest in MS medium supplemented with 1 mg/l 2,4-D. Callus growth was excellent in 2% sucrose, but it was inhibited in propotion to sucrose content. Effect of gelling agents on callus growth was highest on 1.2% agar and 0.4% Gelrite medium, respectively. The browning of callus was protected on the media supplemented with 10 mg/l ABA and 5 or 10 mg/l $AgNO_3$. In the callus induction and growth from the peduncle of immature inflorescence, 2,4-D was more effective than NAA, and the frequency of callus induction was highest as 81.7% in 2 mg/l 2,4-D. Plant was not regenerated from the callus derived from young leaf. Somatic embryos were developed from the surface of callus drived from the peduncle of immature inflorescence in the medium containing 0.5 mg/l 2,4-D, 1 mg/l kinetin, 5 mg/l ABA and 5 mg/l $AgNO_3$. Plants were developed from the matured somatic embryos in the medium supplemented with 0.2 mg/l 2,4-D and 1 mg/l kinetin.

• Journal of Ginseng Research
• /
• v.23 no.3 s.55
• /
• pp.176-181
• /
• 1999

The purpose of this study was to investigate the antioxidant activities of water soluble browning reaction products (WS-BRPs) isolated from korea red ginseng. Antioxidant activities of WS-BRPs were examined with the various systems. All three WS-BRPs (L, S-1 and S-2) were found to have an ability to donate hydrogen to DPPH. Especially, L was more effective than S-1, S-2. and, L as well as S-1 and S-2 was the strongest than BHT, BHA $(5{\times}10^{-4}M),\;{\alpha}-tocopherol\;(1.0{\times}10^{-4})$ and ascorbic acid $(5.7{\times}10^{-3}M)$ previously known as antioxidants. These WS-BRPs (L, S-1 and S-2) also showed a synergistic effect against antioxidative activities of these antioxidants. Moreover S-2 had the strongest activity of these three WS-BRPs to scavenge free radicals such as hydrogen peroxide $(H_2O_2)$.