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De novo Regeneration of Fertile Common Bean (Phaseolus vulgaris L.) Plants  

Albino Margareth M.C. (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes, Universidade de Brasilia, Departamento de Botanica, Instituto de Ciencias Biologicas)
Vianna Giovanni R. (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes)
Falcao Rosana (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes)
Aragao Francisco J.L. (Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes)
Publication Information
Journal of Plant Biotechnology / v.7, no.4, 2005 , pp. 267-272 More about this Journal
Abstract
Common bean (Phaseolus vulgaris L.) plants were regenerated via organogenesis from mature embryonic axes, cultured on MS medium supplemented with ildole-3-ecetic acid (IAA) and thidiazuron (TDZ) for one week in the dark. Embryonic axillary regions were excised, longitudinally cut to split the both sides, and cultured for two weeks on MS medium supplemented with IAA and TDZ. The combination 0.5 mg $l^{-1}$ TDZ/0.5 mg $l^{-1}$ IAA presented the higher efficiency in shoot regeneration and the combination 0.5 mg $l^{-1}$ TDZ/0.25 mg $l^{-1}$ IAA presented the higher efficiency in conversion of shoots to plants. Regenerating explants were transferred to MS medium containing 1 mg $l^{-1}$ BAP for shoot development. All elongated shoots were rooted in vitro, presented normal phenotype and produced viable seeds. Histological analysis confirmed the mode of regeneration as de novo shoot organogenesis.
Keywords
gene expression; gene transfer; organogenesis; regeneration; shoot formation;
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1 Akasaka Y, Daimon H, Mii M (2000) Improved plant regeneration from cultured leaf segments in peanut (Arachis hypogaea L.) by limited exposure to thidiazuron. Plant Sci 156: 169-175   DOI   ScienceOn
2 Aragao FJL, Barros LMG, Brasileiro ACM, Ribeiro SG, Smith FD, Sanford JC, Faria JC, Rech EL (1996) Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. Theor Appl Gent 93:142-150   DOI   ScienceOn
3 Aragão FJL, Vianna GR, Albino MMC, Rech EL (2002) Transgenic dry bean tolerant to the herbicide glufosinate ammonium. Crop Sci 42:1298-1302   DOI
4 Bajaj YPS, Dhanju MS (1979) Regeneration of plants from apical meristem tips of some grain legumes. Curr Sci 48: 906-907
5 Bohmer P, Meyer B, Jacobsen HJ (1995) Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Rep 15: 26-29   DOI   ScienceOn
6 Cruz de Carvalho MH, Van Le B, Zuily-Fodil Y, Pham Thi AT, Tran Thanh Van K (2000) Efficient whole plant regeneration of common bean (Phaseolus vulgaris L.) using thincell-layer culture and silver nitrate. Plant Sci 159: 223-232   DOI   ScienceOn
7 Franklin CI, Trieu TN, Gonzales RA, Dixon RA (1991) Plant regeneration from seedlings explants of green bean (Phaseolus vulgaris L.) via organogenesis. Plant Cell Tissue Organ Cult 24: 199-206   DOI
8 Malik KA, Saxena PK (1992) Thidiazuron induces high frequency shoot regeneration in intact seedling of pea (Pisum sativum), chickpea (Cicer arietinum L.) and lentil (Lens culinaris). Aust J Plant Physiol 19: 731-740   DOI
9 McClean P, Grafton KF (1989) Regeneration of dry bean (Phaseolus vulgaris) vis organogenesis. Plant Sci 60: 117-122   DOI   ScienceOn
10 Russel DR, Wallace KM, Bathe JH, Martinell BJ, McCabe DE (1993) Stable transformation of Phaseolus vulgaris via electric-discharge mediated particle acceleration. Plant Cell Rep 12: 165-169
11 Sanford JC, Devit MJ, Russel JA, Smith FD, Harpending PR, Roy MK, Johnston AS (1991) An improved helium driven biolistic device. Technique 3: 3-16
12 Svetleva D, Velcheva M, Bhowmik G (2003) Biotechnology as a useful tool in common bean (Phaseolus vulgaris L.) improvement. Euphytica131: 189-200   DOI   ScienceOn
13 Sylvester AC, Ruzin.SE (1994) Light microscopy: I. Dissection and microtechnique. In Freeling M, Walbot V (eds), The Maize Handbook, Springer-Verlag New York, pp. 83-95
14 Kanyand M, Dessai AP, Prakash CS (1994) Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro. Plant Cell Rep 14: 1-5
15 Fakhrai F, Evans PK (1989) Morphogenic potential of cultured explants of Crocus chrysanthus Herbert. cv. E. P. Bowles. J Exp Bot 40: 809-812   DOI
16 Aragao FJL, Ribeiro SG, Barros LMG, Brasileiro ACM, Maxwell DP, Rech EL, Faria JC (1998) Transgenic beans (Phaseolus vulqaris L.) engineered to express viral antisense RNAs show delayed and attenuated symptoms to bean golden mosaic geminivirus. Mol Breed 4: 491-499   DOI   ScienceOn
17 Zambre MA, De Clercq J, Vranova E, Van Montagu M, Angenon G, Dillen W (1998.) Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (terapy bean). Plant Cell Rep 17: 626-630   DOI   ScienceOn
18 Kartha KK, Pahl K, Leung NL, Mroginski LA (1991) Plant regeneration from meristems of grain legumes: soybean, peanut, chickpea and bean. Can J Bot 59:1671-1679
19 Pickardt T (2003) In vitro regeneration and genetic transformation of Vicia species. In Jaiwal PK, Singh RP (eds), Applied Genetics of Leguminosae Biotechnology, Kluwer Academic Publisher, Dordrecht, pp. 121-131
20 Sugiyama M (1999) Organogenesis in vitro. Curr Opi Plant Biol 2: 61-64   DOI   PUBMED   ScienceOn
21 Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: $\beta$-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907
22 Datla RS, Hammerlindl JK, Pelcher LE, Crosby WL, Selvaraj G (1991) A bifunctional fusion between beta-glucuronidase and neomycin phosphotransferase: a broad-spectrum marker enzyme for plants. Gene 101: 239-146   DOI   PUBMED   ScienceOn
23 Saunders JW, Hosfield GL, Levi A (1987) Morphogenetic effects of 2,4-dichlorophenoxyacetic acid on pinto bean (Phaseolus vulgaris L.) leaf explants in vitro. Plant Cell Rep 6: 46-49   DOI   ScienceOn
24 Allavena A (1984) Beans (Phaseolus). In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds), Handbook of Plant Cell Culture, Vol. 2, Macmillan Publishing CO., New York, pp137-168
25 Eapen S, Tivarekar S, George L (1998) Thidiazuron-induced shoot regeneration in pigeonpea (Cajanus cajan L. Millsp.). Plant Cell Tiss Org Cult 53: 217-220   DOI   ScienceOn
26 Joersbo M, Brunstedt J, Marcussen J, Okkels, FT (1999) Transformation of the endospermous legumeguar (Cyamopsis tetragonoloba L.) and analysis of transgene transmission. Mol Breed 5: 521-529   DOI   ScienceOn
27 Nagl W, Ignacimuthu S, Becker J (1997) Genetic engineering and regeneration of Phaseolus and Vigna. State of the art and new attempts. J Plant Physiol 150: 625-644   DOI   ScienceOn
28 Sen J, Guha-mukherjee S (1998) In vitro induction of multiple shoots and plant regeneration in Vigna. In Vitro Cell Dev Biol-Plant 34: 276-280   DOI
29 Singh ND, Lingaraj S, Neere BS, Pawan KJ (2003) The effect of TDZ on organogenesis and somtic embryogenesis in pigeonpea (Cajanus cajan L. Millsp). Plant Sci 164: 341-347   DOI   ScienceOn
30 Genga A, Allavena A (1991) Factors affecting morphogenesis from immature cotyledons of Phaseolus coccineus L. Plant Cell Tiss Org Cult 27:189-196   DOI
31 Martins IS, Sondahl MR (1984) Multiple shoot formation from shoot apex cultures of Phaseolus vulgaris. J Plant Physiol 115:205-208   DOI
32 Mohamed MF, Coyne DP, Read PE (1993) Shoot organo- genesis in callus induced from pedicel explants of common bean (Phaseolus vulgaris). J Am Soc Hort Sci 118:158-162
33 Zambre M, Geerts P, Maquet A, Van Montagu M, Dillen W, Angenon G (2001) Regeneration of fertile plants from callus in Phaseolus polyanthus Greenman (year bean). Ann Bot 88: 371-377   DOI   ScienceOn
34 Kumar AS, Gamborg OL, Nabors MW (1988) Regeneration from long-term cell suspension cultures of tepary bean (Phaseolus acutifolius). Plant Cell Rep 7: 322-325   DOI   ScienceOn
35 Dillen W, De Clercq J, Van Montagu M, Angenon G (1996) Plant regeneration from callus in a range of Phaseolus acutifolius A. Gray genotypes. Plant Sci 118: 81-88   DOI   ScienceOn
36 Kanyand M, Peterson CM, Prakash CS (1997) The differentiation of emergences into adventitious shoots in peanut, Arachis hypogea (L.). Plant Sci 126: 87-95   DOI   ScienceOn
37 Allavena A, Rossetti L (1983) Efforts in somatic embryogenesis of Phaseolus vulgaris L. Acta Hort 131: 239-246
38 Aragao FJL (2003) Genetic transformation of common bean via particle bombardment. In Jaiwal PK, Singh RP (eds), Applied Genetics of Leguminosae Biotechnology, Kluwer Academic Publisher, Dordrecht, pp. 35-45
39 Mohamed MF, Read PE, Coyne DP (1992) Plant regeneration from in vitro culture of embryonic axis explant in common bean and terapy bean. J Am Soc Hort Sci 117:332-336