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Effects of Plant Age and Cultural Conditions on Leaf Shape of Korean Ginseng (년생 및 재배조건이 인삼의 엽형에 미치는 영향)

  • 이종철;최창렬
    • Journal of Ginseng Research
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    • v.8 no.2
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    • pp.178-183
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    • 1984
  • This study was conducted to know the influence of plant age and cultural conditions such as plant population density and light intensity under the shading on the leaf shape of panax ginseng. The result obtained were as follows; 1) Leaf length(L)/maximum width(W) was no difference with different age of over 3-over 3year old plant, but that of 1 or 2-yearold was smaller than those of over 3-year old. The values of L/W showed in the order of 2 or 4,3, 1 or 5 leaflet. 2) Ratios of leaflet area to leaf area were 32.0% in leaflet 3, from 209.% to 27.9% in leaflet 2 or 4, and from 6.5% to 7.1% in leaflet 1 or 5. 3) The coefficients of variability for L/W and ratio of leaflet area to leaf area of leaflet 3 were smallest among leaflets. 4) There were significant differences between largest and smallest leaflet 3, leaf areas and ratio of leaflet 3 area to leaf area in same plant. 5) LW and ratio of leaflet 3 area to leaf area were not affected by plant population density. 6) It showed a tendency that the L/W was increased with increasing the light transmittance rate (LTR). The ratio of leaflet 3 area to leaf area of ginseng grown under 20% LTR was not different comparing to that of plant grown under 5% LTR, but it was significantly increased in plant grown at 100% LTR.

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Effects of Photoperiod on Oviposition and Emergence of Egg Parasitoid, Trichogramma dendrolimi Mastumura (Hymenoptera, Trichogrammatidae) (광조건이 송충알벌(벌목, 알벌과)의 산란과 우화에 미치는 영향)

  • 박영규;이해풍;이기상;한만위;이정운
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.93-99
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    • 1999
  • We investigated the effect of constant photoperiod (16L : 8D, 12L : 12D, 8L : 16D), on the oviposition, parasitism, and emergence of Trichogramma dendrolimi. Also during 16L : 8D condition circadian, oviposition and emergence of this species on artificial eggs were checked every 2hrs. When twelve eggs of Antheraea pern~i and artificial eggs were provided, the wasps had the highest rate of oviposition in the first day, and decreased dramatically there after. In these different photoperiod, the total oviposition numbers per A. pernyi eggs by the wasp were 161.8 in 16L : 8D, 145.8 in 121 : 12D and 128.5 in 8L : 16D respectively in the first day. On the other hand for the artificial egg in 16L : 8D photoperiod, the total oviposition number per egg was 106.8 in the first day, 26.6 in the 2nd day and 21.3 in the 3rd day. The rate of emergence from A. pernyi eggs was not different depend on wasp's day age, however in the artificial eggs, after the first day's 84.7% decreased considerably. The emergence rate from artificial eggs was the highest comparatively between 04:OO-0600 hours.

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The Effect of Nutritional Balance between Carbon and other Nutrient Sources on the Growth of Sporobolomyces holsaticus (탄소원과 다른 영양원간의 영양균형이 Sporobolomyces holsaticus의 균체생육도에 미치는 영향)

  • Park, Wan-Soo;Koo, Young-Jo;Shin, Dong-Hwa;Min, Byung-Yong
    • Korean Journal of Food Science and Technology
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    • v.15 no.1
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    • pp.56-61
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    • 1983
  • Direct production of biomass from starch using amylolytic yeast, Sporobolomyces holsaticus FRI Y-5 was studied with varying the ratios between carbon and other nutrient sources in the medium. It was investigated under condition of constant C/P and C/S ratio to influence the initial concentration of starch $(S_o)$ and C/N ratio on its growth which is described as the specific growth rate $({\mu})$, cell yield (Y), the maximum concentration of cell $(X_m)$, and productivity (P). They were very dependent on both $S_o$ and C/N ratio. The form of the relationship between and ${\mu}$ and $S_o$ was observed to be similar to saturation kinetics at C/N = 100 but presented substrate inhibition at other C/N ratios. As $S_o$ was changed from 22.5 to 90 g/l, Y was observed to vary with C/N ratios but seemed to decrease as a wholes. $X_m$ was linearly related to $S_o$ at more than C/N = 50 but at less than C/N = 10 substrate inhibition was presented. P increased suddenly to $S_o$ = 45 g/l and then changed decreasingly at less than C/N = 50, but at more than C/N = 100 it changed increasingly. The effect of C/P ratio and C/S ratio on the yeast growth was also investigated at constant $S_o$ and C/N ratio. ${\mu}$ was dependent on C/P and C/S ratios, but Y, independent on them. But $X_m$ was reliant upon C/P ratio but not upon C/S ratio.

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Complexation of Cadmium(Ⅱ) with Soil Fulvic Acid : Effect of pH and Fulvic Acid Concentration (풀빅산과 카드뮴(Ⅱ)과의 착화합 반응 : 풀빅산의 농도와 pH의 영향)

  • Choe, Se Yeong;Jeong, Geun Ho;Jeon, Song Hui;Mun, Hui Jeong
    • Journal of the Korean Chemical Society
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    • v.38 no.8
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    • pp.585-589
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    • 1994
  • The complexation of cadmium(II) with a well characterized local soil fulvic acid was investigated at different solution pH and fulvic acid concentration using ion selective electrode. The stability constants were determined using a continuous distribution model based on Scatchard Plot; this model takes into consideration the diversitv of cation binding sites on a naturally occurring organic macromolecules even if the nature of the binding sites may not be known. The mean value of $logKi(\mu)$ was found to increase at higher pH(pH 4.0: ${\mu}=3.79{\pm}0.74$ l\;mol^{-1}; pH 6.0: ${\mu}=4.51{\pm}0.78$ l\;mol^{-1}$), and in more dilute fulvic acid concentration([FA] = 50 mgl$^{-1}: {\mu}=4.16{\pm}0.60$ l\;mol^{-1}; [FA]=151 mgl$^{-1}:{\mu}=3.75{\pm}0.5\;l\;mol^{-1}$). The intrinsic constant(logKint) for binding at the strongest site was measured to be around $4.72\;l\;mol^{-1}$ at both pH4.0 and 5.0, but was found to be increased to $6.03\;l\;mol^{-1}$ when pH was 6.0.

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The Effect of Bacillus-Fermented Scutellariae Radix Acupuncture Solution on Interleukin Production in Mouse Macrophage Stimulated by Lipopolysaccharide (바실러스균 발효황금약침액이 Lipopolysaccharide로 활성화된 마우스 대식세포의 인터루킨 생성에 미치는 영향)

  • Park, Wan-Su
    • Korean Journal of Acupuncture
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    • v.27 no.2
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    • pp.95-105
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    • 2010
  • Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on interleukin(IL) production in mouse macrophage stimulatedby lipopolysaccaride(LPS). Methods : Productions of interleukins were measured y High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$(multi-analyte profiling beads) technology. To begin with, cell culture supernatant was obtained after treatment with LPS(1 ${\mu}g/mL$) and SB for 24 hour. Then, it was incubated with the antibody-conj${\mu}g$ated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. After incubating for 30 minutes, Strepavidin-conjugated Phycoerythrin(SAPE) was then added. Incubating for another 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Results : The results of the experiment are as follows. SB significantly inhibited the LPS-induced production of IL-3($9.15{\pm}0.35$ pg/mL) by $6.92{\pm}0.05,\;7.21{\pm}0.11,\;6.96{\pm}0.33,\;and\;7.45{\pm}0.74$ pg/mL at the concentration of 25, 50, 100, and 200 ${\mu}g/mL$ in mouse macrophage RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced production of IL-5($7.30{\pm}0.48$ pg/mL) by $6.50{\pm}0.29,\;6.30{\pm}0.25,\;6.30{\pm}0.25,\;and\;5.80{\pm}0.25$ pg/mL at the concentration of 25, 50 100, and 200 ${\mg}g/mL$ in RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced productiion of IL-9($17.26{\pm}0.19$ pg/mL) by $15.01{\pm}0.43$ pg/mL at the concentration of 25 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced productioh of IL-13($187.80{\pm}2.90$ pg/mL) by $152.80{\pm}4.25,\;172.80{\pm}3.97,\;162.10{\pm}6.67,\;and\;165.30{\pm}11.80$ pg/mL at the concentration fo 25, 50, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-17($18.30{\pm}0.95$ pg/mL) by $13.30{\pm}1.25,\;13.80{\pm}1.11,\;13.30{\pm}0.75,\;and\;14.00{\pm}1.08$ pg/mL at the concentration of 25, 50 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-23($43.90{\pm}0.83$ pg/mL by $39.50{\pm}1.26,\;38.00{\pm}1.78,\;and\;39.60{\pm}2.49$ pg/mL at the concentration of 25, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). Conclusions : These results suggest that SB has anti-inflammatory activity related with its inhibition of IL-3, IL-5, IL-13, IL-17, and IL-23 production in macrophages.

Aquaculture Recycling Effluent from a Pond System Treating Animal Excreta Ecologically (축산폐수 처리 연못시스템의 처리수 재활용 양어)

  • Yang, Hong-Mo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.4
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    • pp.339-344
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    • 2000
  • Utilization of animal excreta in aquaculture can have potentials of high fish production and low maintenance costs for fish farming and it can reduce water pollution caused by animal waste disposal. Integration of wastewater treatment pond system with aquaculture has been utilized in many countries. Ecologically balanced pond ecosystem is formed through the stabilization of wastes, the growth of aquatic plants, and the cultivation of fish. The most appropriate fish for rearing in these ponds are those which can feed directly on phytoplankton, especially algae. Carp were introduced into a tertiary pond - water depth of 2.2 m, water surface area of $130\;m^2$, volume of $148\;m^3$ - of a pond system treating milk cow excreta. The carp production was $125g{\cdot}m^{-2}year^{-1}$ which falls into upper range of $18\;-\;137g{\cdot}m^{-2}year^{-1}$ of treated sewage-fed carp farming of other countries. Average $BOD_5$ and T-N of the pond was 19.8 and $21.0\;mg{\cdot}L^{-1}$ respectively, and the ecological environment of it was suitable for growth of carp. Several carp of 100g were introduced in August into a secondary pond of the treatment system, whose average $BOD_5$ and T-N was 27.9 and $30.8\;mg{\cdot}L^{-1}$ respectively. They were died within one week, which may be attributed to the depletion of dissolved oxygen at dawn. Effluents from primary treatment can be used in fish pond with dilution and those from secondary treatment can be directly funnelled into it. Waste stabilization pond treating animal excreta can be utilized for fish rearing when its water quality maintains secondary treatment level.

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Effect of noise and reverberation on subjective measure of speech transmission performance for elderly person with hearing loss in residential space (주거 공간에서 고령자 청력손실을 고려한 소음 및 잔향에 따른 음성 전송 성능의 주관적 평가)

  • Oh, Yang Ki;Ryu, Jong-Kwan;Song, Han-Sol
    • The Journal of the Acoustical Society of Korea
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    • v.37 no.5
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    • pp.369-377
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    • 2018
  • This study investigated the effect of noise and reverberation on subjective measure of speech transmission performance for elderly person with hearing loss in residential space through listening test. Floor impact, road traffic, airborne, and drainage noise were employed as the residential noise, and several impulse responses were obtained through room acoustical computer simulation for an apartment building. Sound sources for the listening test consisted of residential noises and speech sounds for boh the young (the original sound) and the aged (the sound filtered out by filters with frequency responses of hearing loss of 65 years elderly person). In the listening test, subjects evaluated speech intelligibility and listening difficulty for the presented word ($L_{Aeq}$ 55 dB) at three noise levels ($L_{Aeq}$ 30, 40, 50 dB) and three reverberation times (0.5, 1.0, 1.5 s). Results showed that the residential space with noise level lower than equal to 50 dB ($L_{i,Fmax,AW}$) for jumping noise and 40 dB ($L_{Aeq}$) for road traffic, airborne, and drainage noise had speech intelligibility of 90 % and over and listening difficulty of 30 % and below. Speech intelligibility and listening difficulty for the aged sound source was shown to be 0 % ~ 5 % lower and 2 % ~ 20 % higher than those for the young sound source, respectively.

Study of dynamic mechanical behavior of aluminum 7075-T6 with respect to diameters and L/D ratios using Split Hopkinson Pressure Bar (SHPB)

  • Kim, Eunhye;Changani, Hossein
    • Structural Engineering and Mechanics
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    • v.55 no.4
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    • pp.857-869
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    • 2015
  • The aluminum 7075-T6 is known as an alloy widely used in aircraft structural applications, which does not exhibit strain rate sensitivity during dynamic compressive tests. Despite mechanical importance of the material, there is not enough attention to determine appropriate sample dimensions such as a sample diameter relative to the device bar diameter and sample length to diameter (L/D) ratio for dynamic tests and how these two parameters can change mechanical behaviors of the sample under dynamic loading condition. In this study, various samples which have different diameters of 31.8, 25.4, 15.9, and 9.5 mm and sample L/D ratios of 2.0, 1.5, 1.0, 0.5, and 0.25 were tested using Split Hopkinson Pressure Bar (SHPB), as this testing device is proper to characterize mechanical behaviors of solid materials at high strain rates. The mechanical behavior of this alloy was examined under ${\sim}200-5,500s^{-1}$ dynamic strain rate. Aluminum samples of 2.0, 1.5 and 1.0 of L/D ratios were well fitted into the stress-strain curve, Madison and Green's diagram, regardless of the sample diameters. Also, the 0.5 and 0.25 L/D ratio samples having the diameter of 31.8 and 25.4 mm followed the stress-strain curve. As results, larger samples (31.8 and 25.4 mm) in diameters followed the stress-strain curve regardless of the L/D ratios, whereas the 0.5 and 0.25 L/D ratios of small diameter sample (15.9 and 9.5 mm) did not follow the stress-strain diagram but significantly deviate from the diagram. Our results indicate that the L/D ratio is important determinant in stress-strain responses under the SHPB test when the sample diameter is small relative to the test bar diameter (31.8 mm), but when sample diameter is close to the bar diameter, L/D ratio does not significantly affect the stress-strain responses. This suggests that the areal mismatch (non-contact area of the testing bar) between the sample and the bar can misrepresent mechanical behaviors of the aluminum 7075-T6 at the dynamic loading condition.

Effect of Angiotensin-I Converting Enzyme Inhibitory from Hydrolysate of Soybean Protein Isolate (분리대두단백질 가수분해물의 Angiotensin-I Converting Enzyme 저해효과)

  • Back, Su-Yeon;Do, Jeong-Ryong;Do, Gun-Pyo;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.8-13
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    • 2010
  • The angiotensin converting enzyme (ACE) inhibition effect of soybean protein isolate hydrolysate was studied using protease. Soybean protein isolate was hydrolysed by seven enzymes (Alcalase 2.4 L, Flavourzyme 500 MG, GC 106, Multifect Neutral, Neutrase 0.8 L, Papain 30,000 and Protamex), enzyme concentrations (0, 0.5, 1.0 and 1.5%), at various hydrolysis times (0, 1, 2, 3, 4, 5 and 6 hr) and suspension concentrations (1, 5, 7, 10 and 15%). Absorbance at 280 nm, brix and ACE inhibitory activity of soybean protein isolate hydrolysates were investigated. Absorbance at 280 nm and brix of Alcalase 2.4 L treatment were higher than other enzyme treatments. The optimum condition of hydrolysis was Alcalase 2.4 L, 1% enzyme concentration, 5% suspension concentration for 4 hr. $IC_{50}$ value of ACE inhibitory activity of soybean protein isolate hydrolysate was $79.94 {\mu}g/mL$. These results suggest that soybean isolate protein hydrolysate from Alcalase 2.4 L may be of benefit for developing antihypertensive therapeutics.

Selection of Glyphosate Resistant Cell Lines in Alfalfa (Medicago sativa L.) (알팔파의 Glyphosate 저항성 세포주 선발)

  • 류점호
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.6
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    • pp.341-344
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    • 1997
  • Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.

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