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Effect of Photoperiods on Sexual Maturation in Spotted seabass, Lateolabrax maculatus (점농어 Lateolabrax maculatus의 성 성숙에 미치는 광주기의 영향)

  • Lim, Sang-Gu;Kim, Young-Soo;Baek, Jae-Min;Kim, Jong-Hyun;Han, Hyoung-Kyun;Cho, Yong-Chul;Kang, Yong-Jin
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.5
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    • pp.935-940
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    • 2014
  • To investigate effect of on sexual maturation in spotted seabass, Lateolabrax maculatus the sexual maturation, photoperiods were established to natural photoperiod, 9L:15D, 11L:13D, 13L:11D and 15L:9D. After rearing for 90 days from August 11 to November 10, gonadosomatic index (GSI) and hormones were analyzed. In the female, GSI was $1.07{\pm}0.09%$ in the natural photoperiod, $1.93{\pm}0.06%$ in 9L:15D, while the GSI of male was $0.75{\pm}0.05%$ in the natural photoperiod, $1.89{\pm}0.12%$ in 9L:15D and $0.22{\pm}0.05%$ in 15L:9D and the lowest in 15L:9D, respectively. The blood estradiol (E2) of female was $226.0{\pm}13.6pg/ml$ in the natural photoperiod, and the highest value of $296.3{\pm}15.0pg/ml$ in 9L:15D. The male $17{\alpha}20{\beta}OHP$ was similar to the female with $196.3{\pm}17.0pg/ml$ in the natural photo period and $133.0{\pm}15.5pg/ml$ in 9L:15D. The testosterone (T) of male was $259.3{\pm}33.1pg/ml$ in the natural photoperiod, but the hightest value of $370.5{\pm}27.5pg/ml$ in 9L:15D. These results provide basic information from sexual maturation of spotted seabass.

Micropropagation from Corm Apical Meristems Culture of Freesia refrecta Hybrida (정단 분열 조직배양에 의한 후리지아의 미세번식)

  • 고정애;김명준;김현순;이진재;김영숙
    • Korean Journal of Plant Resources
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    • v.16 no.1
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    • pp.34-39
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    • 2003
  • Corm apical meristem cultures of thirteen glasshouse freesia cultivars were tested to investigate the possibility of micropropagation using MS basal medium supplemented with 2,4-D, NAA(0.1, 0.5, 1.0mg/L, respectively) and BA (0.5∼2.0mg/L). The majority of the tested cultivars could be induced callus and shoot buds in all culture condition. The combinations of NAA and BA appeared superior to that of 2,4-D and BA depending on cultivars for callus induction and shoot formation. Among the cultivars, 'Golden Yellow' showed the highest regeneration capacity on MS media with 0.5mg/L NAA and 1.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoot from calli were obtained through successive subculture on MS medium supplimented with 0.5mg/L BA. In that condition, more than 60 % shoot regeneration and average of 25.1 shoots per explant was achieved. Transformed shoots on half-strength MS medium without plant growth regulators rooted easily.

Nutrient Removal Characteristics on Organic Material Loadings in Submerged Flat Sheet Type Sequencing Batch Membrane Reactor (침지식 평판형 연속회분식 박반응기에서 유입 유기물 부하의 변화에 따른 영양염류의 제거 특성)

  • Kim, Seung-Geon;Lee, Ho-Won;Kang, Yeung-Joo
    • Membrane Journal
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    • v.20 no.3
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    • pp.241-248
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    • 2010
  • The effect of organic material loadings on nutrient removal characteristics were investigated in sequencing batch reactor, in which a flat sheet type microfiltration membrane with a pore size of $0.4\;{\mu}m$ was submerged. Three organic concentrations of 200 mg/L (Run-1), 400 mg/L (Run-2) and 800 mg/L (Run-3) were carried out continuously to identify their effect on the filtration performance and nutrient removal. The removal efficiencies of T-N and T-P were increased with the increase of COD/N and COD/P. The T-N removal efficiencies of Run-1, Run-2 and Run-3 were 28.1, 32.6 and 90.4%, the average concentrations of T-N in permeate were 32.0, 30.0, and 4.3 mg/L, respectively. The T-P removal efficiencies of Run-1, Run-2 and Run-3 were 13.6, 35.3 and 93.1%, the average concentrations of T-P in permeate were 3.11, 2.33, and 0.25 mg/L, respectively.

Fekete-Szegö Problem for a Generalized Subclass of Analytic Functions

  • Orhan, Halit;Yagmur, Nihat;Caglar, Murat
    • Kyungpook Mathematical Journal
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    • v.53 no.1
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    • pp.13-23
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    • 2013
  • In this present work, the authors obtain Fekete-Szeg$\ddot{o}$ inequality for certain normalized analytic function $f(z)$ defined on the open unit disk for which $$\frac{{\lambda}{\beta}z^3(L(a,c)f(z))^{{\prime}{\prime}{\prime}}+(2{\lambda}{\beta}+{\lambda}-{\beta})z^2(L(a,c)f(z))^{{\prime}{\prime}}+z(L(a,c)f(z))^{{\prime}}}{{\lambda}{\beta}z^2(L(a,c)f(z))^{{\prime}{\prime}}+({\lambda}-{\beta})z(L(a,c)f(z))^{\prime}+(1-{\lambda}+{\beta})(L(a,c)f(z))}\;(0{\leq}{\beta}{\leq}{\lambda}{\leq}1)$$ lies in a region starlike with respect to 1 and is symmetric with respect to the real axis. Also certain applications of the main result for a class of functions defined by Hadamard product (or convolution) are given. As a special case of this result, Fekete-Szeg$\ddot{o}$ inequality for a class of functions defined through fractional derivatives are obtained.

Development of cryogenic liquid-vapor separator and liquid-level meter operating under high pressure condition (고압 상황에서 작동되는 극저온 기액 분리기와 액체 계면 측정기의 개발)

  • Lee, Cheon-Kyu;Hwang, Gyu-Wan;Jeong, Sang-Kwon
    • Progress in Superconductivity and Cryogenics
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    • v.13 no.1
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    • pp.51-55
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    • 2011
  • In this study, the liquid-vapor (L-V) separator equipped with liquid-level meter is developed. In the developed L-V separator, the flange is designed to be attachable so that the separator can be flexibly applied under any cases where the volumetric of L-V separator is varied by the system requirement. The leak-tightness between the attachable flange and the chamber is experimentally confirmed with the use of double groove indium wire sealing even under the high pressure up to 20 bar. In addition, the liquid-level meter is designed and fabricated to figure out the inner state of L-V separator. It consists with 19 carbon composition resistors in series. All resistors are separately calibrated in the temperature range from 77 K to 200 K. The performance of the L-V separator and the liquid-level meter is investigated with experimental tests, and the result is presented in this paper.

Physiological Responses of Starry Flounder Platichthys stellatus during Freshwater Acclimation with Different Speeds in Salinity Change (염분변화 속도를 달리한 담수순화 과정에서 강도다리 Platichthys stellatus의 생리학적 반응)

  • Kim, Young-Soo;Do, Yong-Hyun;Min, Byung-Hwa;Lim, Han-Kyu;Lee, Bok-Kyu;Chang, Young-Jin
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.28-33
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    • 2009
  • Physiological responses (hematological factors, cortisol, glucose, osmolality, $Na^+$, $K^+$ and $Cl^-$) in starry flounder Platichthys stellatus were investigated during freshwater acclimation in the conditions of different speeds in salinity change with acute-decrease (AD) or stepwise-decrease (SD I and II). In AD of acute-decrease salinity, hematocrit (Ht), red blood cell (RBC) and hemoglobin (Hb) were rapidly increased more than SD I of stepwise-decrease salinity. But in case of SD II, Ht, RBC and Hb were no significant difference from beginning to end of this experiments. In AD, cortisol level significantly increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $13.7{\pm}0.2{\mu}g/mL$ at 6 hours and recovered to the basal levels ($3.1{\mu}g/mL$) at 10 days. In SD I, cortisol level was significantly increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $13.6{\pm}0.6{\mu}g/mL$ at 6 hours and recovered to the basal levels ($3.1{\pm}0.4{\mu}g/mL$) at 10 days. In SD II, cortisol level was a little increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $10.5{\pm}2.5$, $10.8{\pm}5.6{\mu}g/mL$ at 6, 12 hours and recovered to the basal level at 48 hours. Glucose level of AD, SD I, II were no significant difference from beginning to end of this experiments. Osmolality was $286.8{\pm}3.3\;mOsm/kg$ at the beginning. In SD II of stepwise-decrease, osmolality was no significant difference during rearing in freshwater (FW). But AD of stepwise-decrease and SD I of stepwise-decrease, osmolality was a little decreased end of this experiments. In AD of acute-decrease, only $Cl^-$ level was showed no significant difference from beginning to end of experiment and $Na^+$, $K^+$ levels were decreased. In case of SD I, $Cl^-$ level was showed no significant difference from beginning to end of experiment and $Na^+$, $K^+$ levels were decreased.

How do dense cores embedded in a pc scale filamentary clouds form, by gas flow motions along filamentary clouds and/or contracting motions by themselves?

  • Kim, Shinyoung;Lee, Chang Won;Myers, Philip C.;Caselli, Paola;Kim, Mi-Ryang
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.41.2-42
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    • 2020
  • Understanding how the filamentary structure plays a role in the formation of the prestellar cores and stars is a key issue to challenge. We have observed two prestellar cores in surrounding filamentary environments in 13CO, C180 (3-2) and HCO+ (4-3) molecular lines with the Heterodyne Array Receiver Program (HARP) of the James Clerk Maxwell Telescope (JCMT), in order to search for the evidence related to the possible flow motions along the filament and/or the radial accretion (or infalling motions) of gas material toward the dense cores from their surrounding filamentary cloud. In L1544, the velocity gradient of 1.6 km s-1 pc-1 toward the core was measured in a small branch of filament lying on a radial direction of main filament while no velocity gradient along the main axis of filament in both 13CO and C18O lines. In L694-2, we found the velocity gradient of 0.6 km s-1 pc-1 along the filament in only 13CO lines. The projected accretion rate of ~6 M◉ Myr-1 was estimated in both cases. The infall (or radially contracting) velocity of gas material was measured ~0.16 km s-1 in both 13CO and HCO+ lines and in both L1544 and L694-2, which leads to estimate a mass infall rate of ~20 M◉ Myr-1. Our analysis suggests that our targets are at a stage where the gravitational contraction dominates the mass accretion through the surrounding filamentary cloud. This is consistent with the fact that our targets are highly evolved prestellar cores on a verge of star formation. More detailed results will be presented at the meeting.

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Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes (에탄올 발효방법에 따른 음식물류 폐기물의 바이오에탄올 생산성 비교)

  • Kang, Hee-Jeong;Li, Hong-Xian;Kim, Yong-Jin;Kim, Seong-Jun
    • KSBB Journal
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    • v.25 no.5
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    • pp.471-477
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    • 2010
  • In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 $h^{-1}$), 6 h (0.07 $h^{-1}$), 3 h (0.13 $h^{-1}$). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 $h^{-1}$.

Effect of Pectinase Treatment on the Extraction Yield Improvement from Rubus coreanus Juice and Physicochemical Characteristics during Alcohol Fermentation (Pectinase 처리가 복분자 과즙의 추출 수율 향상과 알코올 발효 중 이화학적 특성에 미치는 영향)

  • Jeong, Eun-Jeong;Kim, Hyeong-Eun;Shin, Dong-Hwa;Kim, Yong-Suk
    • Food Science and Preservation
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    • v.14 no.6
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    • pp.702-708
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    • 2007
  • The effects of pectinase treatment and other processing conditions on juice yield from Rubus coreanus, and physicochemical changes during alcohol fermentation, were investigated. The yield from R. coreanus increased by 8.60% with Pectinex 100L treatment (500 ppm, 30 min) compared to a control group. The soluble solid content in the group fermented at $24^{\circ}Brix$ by addition of sucrose (24B-group), and the group fermented at $8^{\circ}Brix$ by addition of 16% sucrose after 4 days of fermentation (8B-group) decreased to $8.2{\sim}8.3$ and $7.7{\sim}8.0%$ after 10 days of fermentation, respectively, and no significant differences were observed with Pectinex 100L treatment. Initial titratable acidity in the enzyme treatment was slightly higher ($1.18{\sim}1.22%$) than for the control group (1.02%). The initial $L^*$ and $b^*$ values of R. coreanus juice decreased with enzyme treatment, and the $a^*$ value increased, but the color difference (${\Delta}E$) between the control and enzyme treatment gradually decreased with fermentation time. The ethanol contents in the 24B-group and the 8B-group were $16.01{\sim}16.22%$ and $13.29{\sim}13.52%$, respectively, after 10 days of fermentation. The methanol contents in the enzyme treatment and the control were $0.359{\sim}0.404$ and $0.520{\sim}0.604$ mg/mL, respectively, and within standard regulations (1 mg/mL).