• Bulletin of the Korean Chemical Society
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• 제11권3호
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• pp.206-208
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• 1990

Cu(II) hexaazamacrotricyclic complexes $[Cu(L)](ClO_4)_2$ and $[(Cu(L)Cl]ClO_4$, where L = 1,3,6,9,11,14-hexaazatricyclo$[12.2.1.1^{6,9}]octadecane(L_1)$ or 1,3,6,10,12,15-hexaazatricyclo$[13.3.1.1^{6,10}]eicosane(L_2)$, have been prepared by the simple template condensation reactions of triamines, diethylenetriamine for $L_1$, and N-(2-aminoethyl)-1,3-propanediamine for $L_2$, with formaldehyde in the presence of $Cu(OAc)_2\;or\;CuCl_2$. The Cu(II) complexes of $L_1$ contain two 1,3-diazacyclopentane ring moieties and those of $L_2$ contain two 1,3-diazacyclohexane ring moieties that are fused to the 14-membered macrocyclic framework. Spectra indicate that complexes $[Cu(L)](ClO_4)_2\;and\;[Cu(L)Cl]ClO_4$ have square-planar and square-pyramidal chromophores, respectively. square-planar $[Cu(L)](ClO_4)_2$ are remarkably stable against ligand dissociation in acidic aqueous solutions. Square-pyramidal $[Cu(L)Cl]ClO_4$ complexes dissociate their axial Cl-ligands easily in aqueous solutions to form $[Cu(L)H_2O]^{2+}$ species. Infrared and UV/vis absorption spectra of the Cu(II) complexes reveal that Cu-N interactions and the ligand field strengths are significantly weaker in the complexes of $L_2$ than in the complexes of $L_1$.

• 수산해양기술연구
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• 제39권3호
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• pp.239-248
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• 2003

우리나라 주변해역에 서식하는 주요 어종의 체장식별에 필요한 음향반사강도의 data bank를 구축하기 위한 연구의 일환으로 대형음향수조에서 부세의 체장에 따른 음향반사강도를 측정하고, 그 반사강도의 체장 의존성 및 KRM 산란모텔에 의한 이론 반사강도와 측정치를 비교, 분석한 결과를 요약하면 다음과 같다. 1. 체장의 범위가 21.0-32.5 cm인, 총 13마리의 부세를 대상으로 구한 평균반사강도패턴에서 최대 반사강도는 tilt angle이 -13.35$^{\circ}$ 인 입사방향에서 나타났고, 이 때의 반사강도는 -35.13 ㏈ 이었다. 2. 부세의 체장 (fork length, cm)과 평균반사강도의 사이에는 다음의 회귀직선식이 성립하였다. TS=23. 76log(L) -73.45(r=0.47) TS=20log(L) -67.35이 결과로부터 부세의 반사강도는 체장의 2.376승에 비례하는 경향을 나타내었고, 체장변환계수는 체장의 2승에 근사시켜 구한 값 보다 6.1 ㏈ 더 낮게 나타났다. 이들의 결과는 향후 체장어군탐지기의 체장변환계수의 값으로서 활용될 것으로 판단된다. 3. 평균체장은 25.59 cm인 총 13마리의 부세에 대한 반사강도의 빈도분포로부터 구한 반사강도는 -41.23 ㏈이었고, 이 값으로부터 구한 체장변환계수는 -69.72 ㏈이었다. 4. KRM model에 의한 부세의 이론반사강도는 L/λ 비가 15보다 작은 영역에서는 파동상의 변화를 나타내면서 증가하는 경향을 나타내었고, 15보다 큰 영역에서는 매우 완만하게 증가하는 경향을 나타내었다. 5. 부세의 평균반사강도의 측정치는 KRM 산란모델에 의한 예측치보다 전반적으로 낮은 경향을 나타내었고, 보다 정량적인 분석을 위해서는 광범위한 주파수 범위에 대한 실험이 필요하다고 판단된다.

• 한국자원식물학회지
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• 제20권4호
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• pp.367-374
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• 2007

엽, 엽병과 화뢰 등의 치상 절편체 부위 모두에서 캘러스가 형성되었으며 특히 화뢰에서 캘러스 형성률이 71.6%로 가장 높았다. 화뢰배양시 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ TDZ의 혼용처리구에서 캘러스 형성이 77.8%로 양호했으며 신초 재분화율 역시 42.6%를 보였다. 캘러스 형성과 신초 재분화를 동시에 시킬 때 1/2 MS배지가 효과적이었는데 1/2 MS배지에서 캘러스 형성률은 46.3%, 신초 재분화율은 13.0%,그리고 발근율은 13.0%를 보였다. 캘러스를 통한 신초의 재분화를 위해 1/2 MS배지에 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ BA를 첨가했을 때 신초 재분화율은 74.1%를 보였고 절편체당 신초수는 2.4개로 좋았다. 발근율은 대조구에서 57.8%로 낮았으나 1.5mg ${\cdot}$ $L^{-1}$ NAA 첨가된 배지에서 97.8%로 높았다. 재분화된 기내 식물체를 원예용 상토에 순화했을 때 생존률이 86.1%로 높았으며 초장, 근장, 생체중 역시 양호하였다.

• 한국결정학회지
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• 제8권2호
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• pp.119-123
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• 1997

[Ni(L)]I1 (1) (L:3,5,10,12-Tetramethly-1,4,8,11-tetraazacyclotetradecane) 화합물을 합성하고 구조를 X-선 외귀법으로 규명하였다. 이 화합물은 직방정계, 공간군 Pcab, a=13.293(1) Å, b=28.550(7) Å, c=10.804(1) Å, z=8로 결정화되었다. 이 화합물의 구조는 최소 자승법으로 정밀화하였으며, 최종 신뢰도 R(Rw)값은 1851개의 회석반점에 대하여 0.043 및 0.046이였다. 이화합물의 결정구조는 약간 일그러진 반면 사각구조와 trans-III 형태를 갖는다.

• Journal of Applied Biological Chemistry
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• 제56권1호
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• pp.49-51
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• 2013

In a previous study, we reported that 13(E)-labd-13-ene-$8{\alpha}$,15-diol (13E) possesses antiviral and anticancer activities. In this study, the anticancer and antimicrobial activities of 13(E) were evaluated against 4 cancer cell lines and 6 bacteria. 13(E) showed inhibitory effect on a variety of cancer cell lines. The $IC_{50}$ values was 8.3-21.3 ${\mu}g/mL$. 13(E) was the most effective growth inhibitor of murine leukaemia cell lines P388, producing approximately 8.3 ${\mu}g/mL$ of $IC_{50}$ in the cytopathic effect (CPE) method. 13(E) also inhibited the growth of the gram-positive bacteria (Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes) and gram-negative bacteria (Vibrio parahaemolyticus, Escherichia coli and Salmonella enteritidis) with a range of minimum inhibitory concentration (MIC) values from 0.092 to 0.598 mg/mL and gram-negative bacteria were more sensitive to the compound (MIC, 0.092 mg/mL).

• 한국약용작물학회지
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• 제11권1호
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• pp.1-4
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• 2003

천연물로부터 항산화 활성물질을 목적으로 DPPH 자유 라디칼 소거활성 검색법을 이용하여 검색을 실사하고, 유의한 활성을 나타낸 산국 메탄올 추출물로부터 2종의 화합물을 분리하고 기기분석을 이용하여 화합물 1과 2의 구조를 apigenin과 acacetin-7-O-rutinoside (Linarin)으로 동정하였다. 이들 화합물은 각각 13.3 및 $42.1{\mu}g$의 농도에서 DPPH 라디칼을 50% 소거하는 활성을 나타내었으며, 화합물 1은 양성 대조양물인 L-ascorbic acid $(RC_{50}\;=\;13.1{\mu}g)$에 비하여 강한 활성을 나타내었다.

• 대한수의학회지
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• 제35권4호
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• pp.863-868
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• 1995

Electroacupuncture therapy was performed in 2 dogs with thoracolumbar disc disease. Case was a 3-year-old female chihuahua with a calcified disc between the 13th thoracic and 1st lumbar vertebrae. Treatment was done by electroacupuncture at San sho yu, Shen yu, Huan tiao and Fu yang-San yin jiao with the current of 0.5~8 volts, 30 Hz for 30 min. She could pass feces and urine 2 days, and walk 9 days after the beginning of the therapy. Case 2 Was a 6-year-old male cocker spaniel with 4 calcified discs(T12-T13, T13-L1, L2-L3 and L6-L7) and spondylosis(lumbosacral junction). Treatment was done by electroacupuncture at Tian ping, Bai hui and Pung kung yu with the current of 2~4 volts, 30 Hz for 20 min. He could pass feces 6 days, urine 8 days, and stand 21 days, after the beginning of the therapy.

• Bulletin of the Korean Chemical Society
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• 제29권10호
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• pp.1905-1910
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• 2008

The reaction of 3,14-dimethyl-2,6,13,17-tetraazatetracyclo[16.4.0$0^{1.18}.0^{7.12}$]docosane ($L^1$) with formaldehyde in warm methanol yielded 3,14-dimethyl-2,6,13,17-tetraazatetracyclo[16.4.$1^{2.6}.0^{1.18}.0^{7.12}$]tricosane ($L^7$) containing one 1,3-diazacyclohexane subunit. In methanol, $L^7$ readily reacts with $Cu^{2+}$ ion to form [$CuL^7(H_2O)$]$^{2+}$ which is extremely inert against methanolysis. In the solution containing $Ni^{2+}$ ion, however, $L^7$ reacts with methanol to yield [$NiL^3$]$^{2+}$ ($L^3$ = 2-(methoxymethyl)-5,16-dimethyl-2,6,13,17-tetraazatricyclo[16.4.$0^{1.18}.0^{7.12}$]- docosane), in which one N-$CH_2OCH_3$ pendant arm is appended. The copper(II) complex [$CuL^7(H_2O)$]- $(ClO_4)_2{\cdot}3H_2O\;(I{\cdot}3H_2O)$ has a severely distorted trigonal bipyramidal coordination geometry with a 4-5- 6-5 chelate ring sequence. The crystal structure of [$NiL^3$]$(PF_6)_2{\cdot}2H_2O$ (IIb) shows that the N-$CH_2OCH_3$ pendant arm is not coordinated to the metal ion in the solid state. In various solvents (S), however, the nickel(II) complex exists as a mixture of [$NiL^3$(S)]$^{2+}$, in which the N-$CH_2OCH_3$ group as well as S is coordinated to the metal ion, and [$NiL^3$]^{2+}.

• 대한본초학회지
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• 제28권1호
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• pp.33-42
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• 2013

Objective : Morus alba Linne Root Bark (MRAL) is a medicinal herb in Korean Medicine, known for its anti-inflammatory and anti-allergic properties. However, its mechanisms of action and the cellular targets have not yet been found and the study was developed to investigate the allergic suppressive effect of MRAL. The purpose of this study is to investigate the allergic suppressive effects of MRAL on activation of MC/9 mast cells. Methods : Cytotoxic activity of MRAL (50, 100, 200, 400 ${\mu}g/mL$) on MC/9 mast cells measured using EZ-Cytox cell viability assay kit (WST reagent). The levels of interleukin-5 (IL-5), IL-13 and IL-4, IL-5, IL-6, IL-13 mRNA expression were measured by enzyme-linked immunosorbent assay (ELISA) and real-time PCR respectively. The expression of transcription factors such as GATA-1, GATA-2, NFAT, AP-1 and NF-${\kappa}B$ p65 DNA binding activity were measured by western blot and electrophoresis mobility shift assay (EMSA). Results : Our results indicated that MRAL (50 ${\mu}g/mL$, 100 ${\mu}g/mL$) significantly inhibited PMA/Ionomycin-induced production of IL-5 and IL-13 and the expression of IL-4, IL-5, IL-6 and IL-13 mRNA in MC/9 mast cells. Moreover, MRAL (50 ${\mu}g/mL$, 100 ${\mu}g/mL$) inhibited PMA/Ionomycin-induced GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos protein expression and NF-${\kappa}B$ p65 DNA binding activity in MC/9 mast cells. Conclusions : In conclusion, we suspect the anti-allergenic activities of MRAL, may be related to the regulation of transcription factors GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF-${\kappa}B$ p65 DNA binding assay causing inhibition of Th2 cytokines IL-5 and IL-13 in mast cells.

• 한국결정학회지
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• 제10권1호
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• pp.33-38
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• 1999

The complex [Ni(L2)(H2O)]Cl·H2O (1) (L2=3,14-dimethyl-2,6,13,17-tetraazartricyclo [14,4,01.18,07.12]docosane-N-acetic acid) has been synthesized and characterized by X-ray crystallography. 1 crystallizes in the triclinic system, space group P, with a=11.274(1), b=13.851(1), c=17.159(6) , α=90.24(2), β=101.10(2), γ=92.11(1)o V=2682.5(11) 3, Z=4, R1=0.042 and wR2=0.111 for 9432 observed reflections with [I>2σ(I)]. The central nicke(II) ion is six-coordinated octahedral geometry with bonds to the four amine nitrogen atoms the carboxylic oxygen atom of the macrocyclic ligand and to the water molecule occupying a position trans to the pendant arm.