• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.234-240
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• 1983

The pathogenicity of free-living amoeba, Waegleria fcwleri, is influenced according to the strain, cultural condition and host (Culbertson et at., 1968; Carter, 1970; Wong et at., 1975), Phillips (1973) demonstrated that Entamoeba histolytica became avirulent after more than 2 year maintenance in axonic culture in vitro. This study was carried out to compare the difference in pathogenicity between two strains of N. fowleri, one of a prolonged maintenance in arsenic medium and the other one obtained by serial brain passage in mice. The 0 strain was that N. fowleri had cultivated axenically more than 7 years in CGVS medium. The 2-1 strain was obtained from the brain of mouse inoculated intranasally with a strain, which was from the mouse brain infected with 0 strain, and cultured for 15 weeks until the beginning of this experiment. White male mice weighing 18-22 g were used. Mice were anesthetized by an intraperitoneal injection of about 1 mg secobarbital, and inoculated intranasally with $10{\times}$10^4 live N. fowleri trophoBoites in a $5{\;}{\mu}l$ cell suspension. Sluggish behaviour, nervousness, rotation and leg paralysis were developed earlier and more frequently in the 2-1 experimental group than the control 0 group. Pathological changes such as inflammatory and necrotic lesion were observed in the olfactory and anterior portion of brain, and these changes were more extensive in the 2-1 group. The edematous and inflammatory changes in lung were demonstrated in mice died after 13th day post-inoculation. The experimental mice of 2-1 group began to die suddenly from 7th day post-inoculation, and the survival time in 2-1 group mice was shorter than 0 group mice. The typical primary amoebic meningoencephalitis was developed in the mice inoculated intranasally with N. fowleri. The prolonged maintenance of N. fowleri amoebae in axonic CGVS medium was observed to have lost their original pathogenicity for mice, but their pathogenicity was restored by serial brain passage in mice.

• Journal of fish pathology
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• v.9 no.1
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• pp.11-13
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• 1996

Ergasilus sieboldi, a parasitic Copepoda, was detected from the gill of Silurus asotus fingerling(7~10 cm long) cultured at a fish farm in Chungnam Province (Fig. 1 and 2). The infected fish did not show any particular external symptoms except a feeble swimming around the boarder of the pond. The isolated parasite was of 1.07 mm in body length with two egg sacks of 1.05 mm and one pair of hook-shaped secondary tentacle. Bychowsky(1963) described that this parasite infected the gill of Salmonidae, Serranidae, Cyprinidae and Siluridae and that the fish in the Baltic Sea, Black Sea. Caspian Sea. Japanese sea zones, Siberian rivers and Lake Bikal were infectd. Bychowsky further pointed out that heavily infected fish with this parasite become emaciated and slow in growth. and often die. As this parasite infected on the peri-branchial area. the infected fish showed a severe disturbance of respiration. Urawa et ai.(1980) studied on the development of Neoergasilus japonicus. an Ergasilidae, which infects Japanese freshwater fish. In Korea, Chun(1985) reported the identification of Ergasilus sieboldi from imported carp. and Neoergasilus and Pseudogasilus japonicus from bluegills. respectively. Chun also identified Pseudoergasilus zacconis in cultured ayu(Plecoglossus altivelis). However. this report deals with the detection of Ergasilus sieboldi from cultured catfish(Silurus asotus) for the first time in Korea.

• 전기의세계
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• v.24 no.4
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• pp.6-10
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• 1975

반도체와 그의 응용소자는 지난 20여년간 눈부식 발전을 이룩하였다. 이는 주로 단결정의 제작기술 진보에 의한 것으로 본다. 그러나 최근 단결정과는 전연 다른 유리질반도체가 국제회의에서도 그 우수성을 의논하기에 이르렀다. 유리질 반도체가 주목을 끌게 된겻은 1968년 Ovshinsky가 "무질서 구조에 있어서 가역적 스위칭현상"이라는 논문이 발표되고 유리질 반도체를 사용한 Ovonic 스위칭 소자의 출현에 기인된다. 유리질 반도체가 전기스위칭 작용, 기억작용을 나타낸다고 하는 Ovshinsky의 발표는 전자제치로서의 응용에 대해 찬반되는 의견이 있었지만 물성적 연구의 교량적인 역할을했다고 할 수 있다. 이런 반도체에 속하는 재료는 호칭도 여러가지로 유리질반도체, 비정질반도체 무정형반도체등으로 불리어진다. 단결정체가 각 격자간에 장거리질서를 갖는 반면 유리질 반도체는 무질서한 구조로 각 격자간에 단거리 질서를 갖는 것이 단결정과는 본질적으로 다른 점이라 본다. 유리 반도체의 종류는 첫째, 원소성 유리반도체로서 Ge, Si, Se, Te 들과 같이 단일원소로 된 겻과, 둘째 IV, V, VI족 원소로 된 공유결합 합금인 As$_{2}$Se$_{3}$-As$_{2}$Te$_{3}$ 계 Ge Si As Te계등의 칼코게나이드 유리등으로 금지대는 어느 것이나 2eV이하이다. 셋째 이론결합인 SiO $Al_{2}$O$_{3}$ Ta$_{2}$O$_{3}$Si$_{3}$N$_{4}$등의 산화물 및 질화물로 대표되는 분자성 비정질 물질로서 금지대는 2eV보다 큰 세종류로 크게 분류할 수 있다. 분류할 수 있다. 한다. 단 개개의 문제에 관한 구체적인 해석 또는 검토에 관하여는 다음 기회에 미루기로하고, 우선 여기서는 당면문제로서 대처하지 않으면 안될 자동주파수제어문제및 계통의 경제운용문제만에 한정하여, 이것을 우리나라의 현상과 관련시켜 개설하고, 이들의 자동화에 관한 기본적인 문제를 간단히 적어 보겠다. 가능하다. 제작완료된 ASIC은 기능시험을 완료했으며 실제 line-of-sight(LOS) 시스템 구현에 적용중이다. 시대를 살아 갈 회원들이다. '컨텐츠의 시대'가 개막되는 것이며, 신세기통신과 SK텔레콤은 선의의 경쟁 과 협력을 통해 이동인터넷 서비스의 컨텐츠를 개발해 나가게 될 것이다. 3배가 높았다. 효소 활성에 필수적인 물의 양에 따른 DIAION WA30의 라세미화 효율에 관하여 실험한 결과, 물의 양이 증가할수록 그 효율은 감소하였다. DIAION WA30을 라세미화 촉매로 사용하여 아이소옥탄 내에서 라세믹 나프록센 2,2,2-트리플로로에틸 씨오에스터의 효소적 DKR 반응을 수행해 보았다. 그 결과 DIAION WA30을 사용하지 않은 경우에 비해 반응 전환율과 생성물의 광학 순도는 급격히 향상되었다. 전통적 광학분할 반응의 최대 50%라는 전환율의 제한이 본 연구에서 찾은 DIAION WA30을 첨가함으로써 성공적으로 극복되었다. 또한 고체 염기촉매인 DIAION WA30의 사용은 라세미화 촉매의 회수 및 재사용이 가능하게 해준다.해준다.다. TN5 세포주를 0.2 L 규모 (1 L spinner flask)oJl에서 세포간의 응집현상 없이 부유배양에 적응,배양시킨 후 세포성장 시기에 따른 발현을 조사한 결과 1 MOI의 감염조건 하에서는 $0.6\times10^6$cell/mL의 early exponential시기의 세포밀도에서 72시간 배양하였을 대 최대 발현양을

• Korean Journal of Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.87-96
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• 1968

As the raw material of fish paste, the experiment on shark meats was conducted from July to December 1967 at the Fisheries Research and Development Agency, Pusan, Korea. The result and the conclusion obtained by the experiment are as follows. 1. Much salt-soluble protein was found in the shark meat comparing with to the other fish. 2. Deodorization of shark meat, using less than $0.05\%$ of acetic acid was most preferable for the elasticity and flexibility of its product. 3. Keeping raw material at low temperature was necessary in maintaining its elasticity. 4. In setting shark fish paste, keeping the raw material at $35-40^{\circ}C$ for 40-70 minutes was the most desirable condition. 5. The best temperature and the duration of heating were 40-60 minutes at $89-90^{\circ}C$, Meanwhile, heating at high temperature was preferable for the storage for a long period, but the elasticity and quality has decreased a little accordingly. 6. For the increase of its palatability, adding $30\%$ of yellow corvenia meat or $20\%$ sea eel meat were more preferable. 7. Pre ervation of the product for 20 days at $20^{\circ}C$ or 50 days at $5^{\circ}C$ was possible by adding $0.2\%$ potassium sorbinate to the product as antiseptic.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.40 no.3
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• pp.176-181
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• 2004

In order to examine the mesh selectivity and optimum mesh size of trap nets for purple shell, Rapane venosa, the field experiments were carried out during Jun. 27 to 29, 2003 at the coastal area of Mal-Do, Kunsan, Jeonbuk province. The experimental fishing gears were used in two set of trap nets, which one set was consisted of 210 trap nets that were coverd with 35mm, 50mm and 65mm in mesh size. The analysis of mesh selectivity curve was done by Kitahara's method. The results obtained are summerized as follows : 1. The total number of catch by the experimental fishing of trap nets for purple shell was 1,682, and it was consisted of 1,268 purple shells (75.4%), 225 Glossaulax reiniana (13.4%), 113 green lings (6.7%) and 76 other fishes (4.5%). 2. The value of maximum 1/m on the mesh selectivity curve was estimated at 1.79. 3. The value of 1/m on the 50% selection range was estimated at 1.24${\sim}$2.72, and the selection width was 1.48.. 4. The optimum mesh size of trap nets for purple shell based on the catch prohibition shell height(50mm) was estimated 40.3mm, and the 50% selection range of shell height of purple shell was 50.0${\sim}$109.6mm.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.2
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• pp.110-116
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• 1970

The fresh_water shrimp, Palaemon modestua is one of the most important types of bait f[r fishing and can be found abundantly in the Nak-Dong River. The larval development of Palaemon modestua in the laboratory was reported by Liu (1949), Kown and Uno (1968), but they didn't give any ecological features in the natural environments. Shrimps were collected from the Nak-Dong River near Pusan, once a month from July to December 1953 and from February to June 1969 respectively. The following is a summary of the results: 1. They breed from April to September, mainly in July. 2. A generation of this type of shrimps appears to be approximately one year and after breeding they die off up untill the end of October. 3. The sex ratio (female=100) is $44.2\~95.5$ (mean 78.5) and goes down during spawning seasons. 4. The minimum size of the ovigerous female is approximately 0.5 gram in body weight, 29mm in body length and 7mm in carapace length. 5. Most shrimps of $31\~41mm$ In body length lay eggs. 6. The relationship between the boby length (X) and the number of eggs (Y) is: $$Y=0.0000203937X^{3.5668}$$ 7. The relationship between the body length (X) and the body weight (Y) is: $$Y=0.099801X^{2.85407}$$ in female. $$Y=0.004198X^{3.40614}$$ in Male. 8. The relationship between the carapace length (X) and the body weight (Y) is: $$Y=5.56008X^{2.25503}$$ in female. $$Y=5.09541X^{2.28875}$$ in male. 9. The relationship between the carapace length (X) and the body length (Y) is: Y= 5.07540+3.36057X in female. Y=4.90514+3.47791X in male.

• 한국해양학회지
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• v.3 no.1
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• pp.16-25
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• 1968

This study was undertaken to assess the annual cycle of primary production and plant pigments in a downstream of the Han River. Measurements were carried out at three week intervals during April 1966 and March 1967, and ancillary data include water temperature, transparency, pH, dissolved oxygen and phytoplankton cell number. The seasonal cycle in water temperature profile shows the hihgest in the end of August with 27$^{\circ}C$, lowest in the middle of February with 0.2$^{\circ}C$. The transparency with Secchi disk reading varied from a maximum 4.0m in fall and a minimum 0.5m or less in early spring and flood season of summer. The pH of the river water varied from 6.5 to 7.3, averaged 6.91 in the surface water and 6.98 in the bottom water, showed little seasonalvariability. The dissolved oxygen in the surface water ranged from 5.93-9.64ml/L, while in the bottom water it ranged from 5.54-9.72 ml/L, and the oxygen saturation never fall below 94%. None thermal, the distribution of pH and content of oxygen, stratifications occurred. An apparent seasonal cycle of primary productivity was observed with remarkably high levels in the spring and fall, the lowest level in the winter. The range of net carbon assimilations showed 3.1-112.6 mgC/㎥/day or 15-427 mgC/㎡/day in spring, 37.0-271.2 mgC/㎥/day or 115-329 mgC/㎡/day in summer, 27.2-168.0 mgC/㎥ /day or 139-415 mgC/㎡/day in fall and 0.5-10.9 mgC/㎥/day or 5-19 mg/㎡/day in winter, respectively. Amount of chlorophyll ${\alpha}$ ranged from a minimum concentration of 0.2-0.3 mg/㎥ in the middle of February and a maximum 4.1-6.7 mg/㎥ in the middle of June. A general increase trend in chlorophyll ${\alpha}$ concentration was noted with increase of the river water temperature.

• 한국해양학회지
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• v.3 no.1
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• pp.26-38
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• 1968

Nutrients and principal ions contents in downstream water of the Han River, near Seoul, measured at somewhat three week intervals over a period of two years were shown its characteristic seasonal cycles. The annual ranges of nutrients were 0.5-7.7 $\mu\textrm{g}$ at/L of ammonia, 0.12-0.90 $\mu\textrm{g}$ at/L of nitrite, 2.9-35.9 $\mu\textrm{g}$ at/L of nitrate, 8.8-42.0 $\mu\textrm{g}$ at/L of tatal soluble nitrogen, 0.09-0.57 $\mu\textrm{g}$ at/L of phosphorus, and 3.0-15.3 mg/L of silicate, and these were lower in spring and fall closely correlate with phytoplankton blooms and higher in winter except ammonia and silicate contents. Annual variations of BOD and COD were 0.10-2.14 ml/L, 0.88-6.82 mg/L, respectively. The ionic concentrations of Cl, Ca and Mg were not high of which Cl 5.0-13.1 mg/L, Ca 3.7-9.1 mg/L and Mg 1.5-10.4 mg/L of annual ranges. Fe ranged 18.5-82.8 $\mu\textrm{g}$/L, Mn 15-58 $\mu\textrm{g}$/L, Cu 0.3-4.7 $\mu\textrm{g}$/L annually In general, the Han River water is low in nutrients, mineral contents, and may be said not yet polluted, excellent quantity and quality of river water.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.4 no.2
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• pp.47-54
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• 1971

The difference in growth rate of p. orientalis cultured on muddy bottom region and sandy bottom region in a pond filled with sea water were studied. For the investigation of the growth rate, the body length and the body weight were determined. Five individuals from each experimental region were sampled in random. The samples were collected at ten-day intervals. The survival rate, the qredator of p. orientalis, and the water conditions during the experimental period were also determined. The results in this study are summerized as follows: 1. The growth rate of p. orientalis which was cultured in the muddy bottom region was greater than that in the sandy bottom region. This is attributable in either case regardless of whether they were cultured with or without food, 2. The relationship between the culturing period (X) and the body length (Y) is : Muddy bottom, Food Y=0.58497X+25.05210 Sandy bottom, Food Y=0.51030X+26.57900 Muddy bottom, No food Y=0.22352X+32.79360 Sandy bottom, No food Y=0.l1418X+31.20740 3. The relationship between the culturing period (X) and the body weight (Y) is : Muddy bottom, Food Y=0.09062X-2.11140 Sandy bottom, Food Y=0.06992X-1.19640 Muddy bottom, No food Y=0.01615X+0.51150 Sandy bottom, No food Y=0.00495X+0.56150 4. When the predator is not present, $84\%$ of P. orientalis may survive. 5. Acanthogobius flavimanus is one of the predator of P. orientalis. 6. It is considered that the variation of water conditions of the pond, in which water had been exchanged 12 times per month, did not affect to the survival rate of P. orientalis.

• Applied Biological Chemistry
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• v.11
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• pp.67-76
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• 1969

The reduced pressure storage of apple, American Summer Pairman, was investigated comparing with the other storage methods and the following results were obtained. 1. The reduced pressure storage was better to extend the storage life and freshness of apples than the controlled atomoshere storage. 2. Decreasing the pressure of chamber atmosphere to about 20 cmHg showed the best result among several pressure conditions. 3. As for the surface treatment of the apples, poly-ethylene film wrapping showed to be the most favorable method in a short-term experiment. In a long-term experiment, however, poly-ethylene film wrapping seemed to cause apples rot. Poly-vinyl acetate coating seemed to keep the apples from color changing, but it caused an unpleasant odor and a peculiar taste. No significant effect was observed in Gibberellin. 4. Total sugar and reduced sugar of apples were decreased after a certain period of increasing. Acid and Vitamin C, however, gradually decreased from the beginning of storage.