• Parasites, Hosts and Diseases
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• 제50권3호
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• pp.263-267
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• 2012

The present study was performed to describe 2 human cases infected by the horsehair worm, Parachordodes sp., in Japan. Two gordiid worms were collected in the vomit and excreta of an 80-year-old woman in November 2009 in Kyoto city, and in the mouth of 1-year-old boy in December 2009 in Nara city, Japan, respectively. Both worms were males having bifurcated posterior ends and male gonads in cross sectional specimens. They were identified as Parachordodes sp. (Nematomorpha: Chordodidae) based on the characteristic morphologies of cross sections and areoles in the cuticle. DNA analysis on 18S rRNA partial sequence arrangements was also carried out and both worms were assumed to be close to the genus Paragordionus based on tree analysis, and far from Gordius sp. which has already been reported in humans in Japan. DNA sequencing of the Parachordodes worm does not appear on the database; therefore, more information on the gene sequences of the genus Parachordodes from humans, animals, or intermediates is required.

• 대한수의학회지
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• 제31권3호
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• pp.311-318
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• 1991

The DNA fragment representing for Pseudorabies gp50 and gp60(Shope) was cloned by recombinant techniques. The viral DNA was extracted from the infected cells and digested with Bam HI. The 6.8 Kb of Bam HI fragment was isolated from agarose gel and further digested with Nde I followed by Klenow treatment. The blunt ended 4.9Kb fragment was cloned into pTZ18R plasmid vector. The upstream region of gp50 was further manipulated to remove its 5' promoter region and create EcoRl site for possible eukaryotic expression system. The result of partial sequencing of cloned DNA indicated that Shope strain showed 95% homology with gp50 of Rice strain.

• Parasites, Hosts and Diseases
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• 제58권6호
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• pp.689-694
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• 2020

Strongyloidiasis is caused by Strongyloides stercoralis and is one of the most neglected tropical diseases in tropical and subtropical regions. Although several strongyloidiasis cases have been reported in Korea, genetic analysis of Korean isolates is still incomplete. In this study, a parasite was isolated from a 61-year-old man diagnosed with strongyloidiasis during the treatment of lymphoma on his retroperitoneal lymph node. Diffuse symmetric wall thickening from the ascending to descending colon and a nematode-infected intestine was observed following microscopic examination. Genomic DNA was isolated from a patient tissue block, and S. stercoralis was identified by PCR and sequencing (18S rDNA). In order to determine phylogenetic location of a Korean isolate (named KS1), we analyzed cox1 gene (500-bp) and compared it with that from 47 previous S. stercoralis isolates (28 human isolates and 19 canid isolates) from Asian countries. Our results showed that phylogenetic tree could clearly be divided into 5 different groups according to hosts and regions. KS1 was most closely related with the Chinese isolates in terms of genetic distance.

• 한국수산과학회지
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• 제50권4호
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• pp.447-452
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• 2017

In this study, we tried to identify a sea anemone collected from the coast of Gijang, Busan. The anemone was morphologically similar to species belonging to the genus Anthopleura, but its morphological characteristics did not allow for confirmed identification to species level. Multiple genes from mitochondrial cytochrome oxidase III, 12S and 16S rRNA, and nuclear 18S and 28S rRNA, were amplified for multilocus sequence typing (MLST) analysis using genomic DNA extracted from the sampled anemone and a different primer set. Based on the MLST analysis, the anemone obtained in this study was identified as Anthopleura artemisia. Also, the sequence of internal transcribed spacer-2 was most closely related to A. artemisia, indicating that this single region might be useful for anemone identification. This study shows significance of molecular identification for sea anemones, and will be helpful in studies of sea anemone identification using genotyping-by-sequencing.

• 한국식품과학회지
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• 제43권6호
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• pp.735-741
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• 2011

메밀 속성장 유래 세포외효소(protease, amylase, cellulase, lipase, xylanase) 분비능이 우수한 균주를 분리하여 혈전 용해능, 항균 활성 및 내염성을 분석하였다. 선발된 균주 중 HJ0-3을 비롯한 13개 균주는 표준 균주 B. subtilis KACC 10114보다 우수한 protease 활성을 보였다. Amylase 분비능의 경우, HJ18-4을 포함한 10개 균주는 표준 균주 B. subtilis KACC 10114보다 우수한 효소 활성을 보였다. HJ11-19 등 5균주는 표준 균주 B. subtilis KACC 10114보다 cellulase 분비능이 우수함을 확인할 수 있었다. 선별된 23개 균주 중에서 HJ5-21(21.12%), HJ18-4(86.11%), HJ18-9(31.16%), HJ18-13(63.80%)가 높은 혈전 용해능을 보였다. 선별된 23개 균주 중, 5개 균주(HJ18-4, HJ18-9, HJ18-13, HJ25-6, HJ25-11)는 3개 이상의 병원성 균에 대해 항균 활성을 나타내었고, 특히 HJ18-4는 모든 병원성 균에 대하여 항균활성을 나타내었으나 표준 균주 B. subtilis KACC 10114는 항균 활성이 나타나지 않았다. 메밀 속성장 유래 분리 균주 중 세포외효소 활성 및 혈전 용해능, 항균 활성이 우수한 균주 HJ18-4(AB601598)는 API kit 분석결과 B. subtilis(89.6%), 16S rDNA 염기서열 분석으로는 B. subtilis(99%)로 동정되었다. HJ18-4는 표준 균주 B. subtilis KACC 10114와 마찬가지로 NaCl의 농도가 높아질수록 생육이 억제되나 5%와 10%의 NaCl 농도에서 표준 균주 B. subtilis KACC 10114 보다 HJ18-4가 우수한 생육 특성을 나타내었다. 이는 HJ18-4가 염 농도 5-10%에서 우수한 생육특성을 가짐으로써 저염 및 고염 장류 제조에 있어서 starter로 사용이 가능함을 보여주었다.

• 한국환경과학회지
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• 제17권4호
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• pp.469-477
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• 2008

The aerobic photosynthetic bacterium, which produces bacterial carotenoids was isolated and identified from coastal marine environments. This bacterium was identified by 16S rDNA sequencing and designated as Erythrobacter longus SY-46. E. longus SY-46 was Gram negative and rod shape, and the optimal culture conditions were $25^{\circ}C$, pH 7.0, and 3.0% NaCl concentration, respectively. The carbon and nitrogen sources required for the optimal growth were lactose and tryptone, respectively. Fatty acid compositions of E. longus SY-46 were $C_{18:1}$(78.32%), v-linolenic acid($C_{18:3n9.12.15c}:3.83%$), margaric acid($C_{17:0}$: 3.38%), palmitic acid($C_{16:0}$: 3.07%), and docosahexaenoic acid($C_{22:6n3}$: 2.21%). In addition, E. longus SY-46 showed the characteristic absorption peaks of bacterial carotenoids(in the region of 450 to 480 nm) and bacteriochlorophyll(770 to 772 nm). Major carotenoids of E. longus SY-46 were polyhydroxylated xanthophylls such as fucoxanthin and zeaxanthin.

• 생명과학회지
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• 제18권1호
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• pp.58-62
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• 2008

기능성 한천올리고당 생산에 사용할 수 있는 유전자원을 확보하기 위하여 동해안 기장 해수에서 한천분해활성을 보이는 해양유래 세균 SL-12를 분리하였으며 165 rDNA염기서열 분석으로 해양기원의 Glaciecola 속과 가장 유사한 균주임을 확인하였다. SL-12 균주가 생성하는 한천분해효소(agarase)의 최적 pH는 pH 7.0 (20 mM sodium phosphate 완충용액)이고 최적 온도는 $30^{\circ}C$로 나타났다. 분리 된 Glaciecola sp. SL-12 균주가 생산하는 한천분해효소의 분해산물에 대한 TLC 분석결과, 기능성 한천올리고당을 생산하는 ${\beta}$-agarase로 판명되어 산업적 활용 가능성이 높은 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제23권7호
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• pp.897-904
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• 2013

Seven bacterial isolates (viz., AB05, AB10, AB11, AB12, AB14, AB15, and AB17) were derived from the rhizosphere and evaluated in terms of plant growth-promoting activities and the inhibition of Phytophthora infestans affecting tomatoes in Korea. According to 16S rDNA sequencing, a majority of the isolates are members of Bacillus, and a single isolate belongs to Paenibacillus. All seven isolates inhibited P. infestans by more than 60% in vitro. However, AB15 was the most effective, inhibiting mycelial growth of the pathogen by more than 80% in vitro and suppressing disease by 74% compared with control plants under greenhouse conditions. In a PGPR assay, all of the bacterial isolates were capable of enhancing different growth parameters (shoot/root length, fresh biomass, dry matter, and chlorophyll content) in comparison with non-inoculated control plants. AB17-treated plants in particular showed the highest enhancement in fresh biomass with 18% and 26% increments in the root and shoot biomass, respectively. However, isolate AB10 showed the highest shoot and root growth with 18% and 26% increments, respectively. Moreover, the total chlorophyll content was 14%~19% higher in treated plants.

• ALGAE
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• 제38권2호
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• pp.127-139
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• 2023

Cryptic stages of diverse macroalgae present in natural substrata, "the bank of microscopic forms", were isolated into clonal cultures and identified based on both morphological characteristics and DNA barcoding. Approximately 120 clonal isolates from 308 natural substratum samples were collected from the entire coastline of Kuwait. Amongst these isolates, 77 (64%) were identified through DNA barcoding using the nuclear ribosomal small subunit, RuBisCO spacer (ITS2, tufa, rbcL, psaA, and psbA) and sequencing. Twenty-six isolates (34%) were identified in the division Chlorophyta, 18 (23%) as Phaeophyceae, and 33 (43%) as Rhodophyta. For all DNA sequences in this study, species-level cut off applied was ≥98% homology which depend entirely on the markers used. Three putative new records of Chlorophyta new for the Arabian Gulf were made: Cladophora laetevirens (Dillwyn) Kützing, Ulva torta (Mertens) Trevisan and Ulvella leptochaete (Huber) R. Nielsen, C. J. O'Kelly & B. Wysor in Nielsen, while Cladophora gracilis Kützing and Ulva ohnoi M. Hiraoka & S. Shimada are new records for Kuwait. For Phaeophyceae, Ectocarpus subulatus Kützing and Elachista stellaris Areschoug were new records for the Gulf and Kuwait. In the Rhodophyta, Acrochaetium secundatum (Lyngbye) Nägeli in Nägeli & Cramer, Ceramium affine Setchell & N. L. Gardner, Gelidium pusillum var. pakistanicum Afaq-Husain & Shameel and Dasya caraibica Børgesen are new records for the Gulf and Kuwait, while the red alga Stylonema alsidii (Zanardini) K. Drew is a new record for Kuwait. Several isolates identified corresponded to genera not previously reported in Kuwait and / or the Arabian Gulf, such as Porphyrostromium Trevisan, a new genus from the Bangiales, and two unidentified species for the Planophilaceae Škaloud & Leliaert. The isolates cultivated from substrata enhance understanding of the marine macroalgal diversity in the region and confirmed that the Germling Emergence Method is suitable for determining the actual diversity of a given study area through isolation from cryptic life-history phases.

• 한국균학회지
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• 제50권4호
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• pp.347-355
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• 2022

A fungal isolate belonging to the phylum Ascomycota was isolated and identified as Talaromyces veerkampii in 2017 during a survey of fungal diversity in field soils in Korea. This fungal isolate was identified and described based on macro- and micromorphological and molecular characterization. The identification was also based on partial 18S-ITS1-5.8S-ITS2-28S rDNA and calmodulin (CaM)-encoding gene sequencing data. Talaromyces veerkampii has not been previously reported in Korea. Thus, we report here a newly discovered species from soil in Korea along with its morphological and molecular characteristics.