• 제목/요약/키워드: 17 $\beta$-estradiol

검색결과 508건 처리시간 0.028초

호르몬을 투여한 Rat 난관상피세포(卵管上皮細胞)의 전자현미경적 관찰 (Electron Microscopic Observations of Oviductal Epithelium of the Rats Treated with Hormone)

  • 이재현
    • 대한수의학회지
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    • 제22권2호
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    • pp.99-109
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    • 1982
  • Morphological changes of the oviductal epithelium of the rat treated with hormones ($17{\beta}$-estradiol ${\mu}g$/day and progesterone 2.5mg/day) for ten days were observed transmission and scanning electron microscopically. The results obtained were as follows: 1. The cilia formation of ciliary cell(CC) was more accelerated by the treatment of estradiol than progesterone, but the balance of estrogen and progesterone was required for the maintenance of CC. The effect of hormone was different between the segments for the maintenance of CC. 2. The short secretory cell(SSC) was severely inhibited in the formation of secretory granules with single hormonal treatment but the activity of secretion was more inhibited by progesterone than by estradiol. 3. The long secretory cell(LSC) had not a great difference between estradiol and progesterone treatments as compared with the normal sexual cycle, but the formation of secretory granules was somewhat accelerated by progesterone treatment. 4. The formation of secretory granules of junctional cell (JC) was severely accelerated by estradiol treatment as compared with the normal sexual cycle. The formation of secretory granules during progesterone treatment, on the other hand, was inhibited completely, but the numbers of pinocytotic vesicles appeared at the cytoplasmic apical portion. 5. Three types of secretory cells, SSC, LSC and JC, on the rat oviductal epithelium could be suggested to have different cell tapes respectively from the morphological changes by hormone treatment.

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17Beta-Estradiol Regulates NUCB2/Nesfatin-1 Expression in Mouse Oviduct

  • Sun, Sojung;Shin, Jungwoo;Jang, Jiwon;Hwang, Seungyeon;Kim, Jeongwoo;Kong, Jinseong;Yang, Hyunwon
    • 한국발생생물학회지:발생과생식
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    • 제24권1호
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    • pp.43-52
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    • 2020
  • NUCB2/nesfatin-1 known to regulate appetite and energy homeostasis is expressed not only in the hypothalamus, but also in various organs and tissues. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the reproductive organs, including the ovaries, uterus, and testes of mice. However, it is yet known whether NUCB2/nesfatin-1 is expressed in the oviduct and how its expression is regulated. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the oviduct and its expression is regulated by gonadotropin. Immunohistochemical staining results showed that nesfatin-1 protein was localized in epithelial cells of the oviduct. As a result of quantitative real-time PCR (qRT-PCR) and Western blot, NUCB2/nesfatin-1 was detected strongly in the oviducts. During the estrus cycle, NUCB2/nesfatin-1 expression in the oviducts was markedly higher in the proestrus stage than in other estrus stages. In order to elucidate whether the expression of NUCB2 mRNA is controlled by the gonadotropins, we injected PMSG and hCG and measured NUCB2 mRNA level in the oviduct after injection. Its level was increased in the oviduct after PMSG injection, but no significant change after hCG injection. In addition, NUCB2 mRNA levels were markedly reduced after ovariectomy, while recovered after 17β-estradiol (E2) injection, but not by progesterone (P4). This study demonstrated that NUCB2/nesfatin-1 is highly expressed in the oviduct of mouse and its expression is regulated by E2 secreted by the ovaries. These results suggest that NUCB2/nesfatin-1 expressed by the oviduct may affect the function of the oviduct regulated by the ovaries.

Influence of 17β-Estradiol on 15-Deoxy-Δ12,14 Prostaglandin J2 -Induced Apoptosis in MCF-7 and MDA-MB-231 Cells

  • Yaacob, Nik Soriani;Nasir, Rabail;Norazmi, Mohd Nor
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권11호
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    • pp.6761-6767
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    • 2013
  • The nuclear receptor, peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$), is expressed in various cancer cells including breast, prostate, colorectal and cervical examples. An endogenous ligand of $PPAR{\gamma}$, 15-deoxy-${\Delta}^{12,14}$ prostaglandin $J_2$ (PGJ2), is emerging as a potent anticancer agent but the exact mechanism has not been fully elucidated, especially in breast cancer. The present study compared the anticancer effects of PGJ2 on estrogen receptor alpha ($ER{\alpha}$)-positive (MCF-7) and $ER{\alpha}$-negative (MDA-MB-231) human breast cancer cells. Based on the reported signalling cross-talk between $ER{\alpha}$ and $ER{\alpha}$, the effect of the $ER{\alpha}$ ligand, $17{\beta}$-estradiol (E2) on the anticancer activities of PGJ2 in both types of cells was also explored. Here we report that PGJ2 inhibited proliferation of both MCF-7 and MDA-MB-231 cells by inducing apoptotic cell death with active involvement of mitochondria. The presence of E2 potentiated PGJ2-induced apoptosis in MCF-7, but not in MDA-MB-231 cells. The $ER{\alpha}$ antagonist, GW9662, failed to block PGJ2-induced activities but potentiated its effects in MCF-7 cells, instead. Interestingly, GW9662 also proved capable of inducing apoptotic cell death. It can be concluded that E2 enhances $ER{\alpha}$-independent anticancer effects of PGJ2 in the presence of its receptor.

Effects of Progesterone and 17β-Estradiol under Presence or Absence of FBS on Plasminogen Activators Activity in Porcine Uterine Epithelial Cells

  • Hwangbo, Yong;Lee, Mi-Rim;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • 한국발생생물학회지:발생과생식
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    • 제22권4호
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    • pp.309-318
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    • 2018
  • The present study was conducted to investigate the regulatory mechanism of plasminogen activators (PAs) activation by $17{\beta}$-estradiol ($E_2$) and progesterone ($P_4$) in porcine uterine epithelial cells (pUECs). pUECs were collected from porcine uterine horn and cultured at 80% confluence. Then, 0.1% (v/v) DMSO, 20 ng/mL $E_2$, and $P_4$ with or without fetal bovine serum (FBS) treated to cultured cells for 24 hours. The supernatants were used for measurement of PAs activity and expression of urokinase-type PA (uPA), tissue-type PA (tPA), uPA specific receptor (uPAR), and type-1 PA inhibitor (PAI-1) mRNA were analyzed by real-time PCR. The expression of PAs-related genes was not affect by steroid hormones in both of serum treatment groups. However, PAs activity was increased by treatment of $E_2$ compared to 0.1% DMSO treatment in serum-free group (p<0.05). Then, $E_2$ and $P_4$ were diluted with 0.002% (v/v) DMSO for reduction of its effect and treated to cultured cells without FBS. Only tPA mRNA was significantly increased by $E_2$ treatment (p<0.05). PAs activity was enhanced in $E_2$ treated group compared to control groups (p<0.05). These results indicate that serum-free condition is more proper to evaluate effect of steroid hormones and activation of PAs in pUECs was mainly regulated by estrogen. These regulation of PAs activation may be associated with uterine remodeling during pre-ovulatory phase in pigs, however, further studies are needed to investigate precise regulatory mechanism.

유우의 번식과정에 따른 유즙중의 성호르몬 수준 변화에 관한 연구 (Studies on the Changes of Sex Hormone Concentrations in Milk during the Reproductive Stages of Dairy Cows)

  • 김상근;이재근
    • 한국가축번식학회지
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    • 제9권1호
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    • pp.9-30
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    • 1985
  • The study was carried out to find out the changes of the sex hormone levels in the milk of Holstein cows during the reproductive stages such as the estrous cycle, pregnancy and periparturient period. The FSH, LH, estradiol-17$\beta$ and progesterone from the milk samples were assayed by radioimmunoassay methods. The results of this study were summarized as follows: 1. The levels of progesterone and estradiol-17$\beta$ were similar among inter-quarters, but they were higher in after milking than before milking times, with no statistical significance. 2. The milk progesterone levels during the estrous cycles reached a peak mean level of 3.55$\pm$0.26ng/$m\ell$ at 15 days after estrus and they did not show any differences among the length of estrous cycles. The estradiol-17$\beta$ levels during the estrous cycles showed a peak level of 36.40$\pm$2.38pg/$m\ell$ at estrus, and decreased(17.20$\pm$0.46 pg/$m\ell$ to 18.65$\pm$1.26pg/$m\ell$) at luteal phase. 3. The FSH levels during the estrous cycles ranged from 2.25$\pm$0.23mIU/$m\ell$ to 4.35$\pm$0.24mIU/$m\ell$ showing significant changes. The LH levels during the estrous cycles gradually increased and remained a peak level of 10.90$\pm$0.36mIU/$m\ell$ from 20 to 25 days after estrus. 4. The progesterone levels during the pregnancy were decreased from 30 to 60 days after artificial insemination, and therafter continuously increased until 240 days. The estradiol-17$\beta$ levels during the pregnancy were 24.56$\pm$1.19pg/$m\ell$ at day 30 after artificial inseminaton, and increased rapidly until 180 days. The levles were agagin decreased by 26.17$\pm$3.03pg/$m\ell$ until 210 days and markedly increased by 68.00$\pm$8.70pg/$m\ell$ until 240 days. 5. The prolactin levels during the pregnancy were 31.27$\pm$2.31ng/$m\ell$ and 42.60$\pm$2.37ng/$m\ell$ at day 150 and 240 after artificial insemination respectively. The LH levels during the pregnancy reached a peak of 27.47$\pm$7.90mIU/$m\ell$ at day 30 after artificial insemination, and thereafter gradually decreased. 6. The progesterone levels during the periparturient period reached a peak of 4.61$\pm$0.34ng/$m\ell$ at day 3 prepartum, and thereafter gradually decreased, and showed 2.05$\pm$0.60ng/$m\ell$ at day 7 postpartum. The estradiol-17$\beta$ levels during the periparturient period showed high level from 207.23$\pm$6.04pg/$m\ell$ at day 1 prepartum to 239.90$\pm$13.90pg/$m\ell$ at day 2 prepartum, and thereafter began to decline and reached 51.87$\pm$1.72pg/$m\ell$ at by 7 postpartum. 7. The prolactin levels during the periparturient period showed relatively higher level at the time of parturition. The LH levels during the periparturient period rnage from 6.32$\pm$0.32mIU/$m\ell$ to 13.90$\pm$1.37mIU/$m\ell$ showing significant changes. 8. The progesterone levels(4.6$\pm$0.8ng/$m\ell$) of the pregnant cows were significantly higher than those (1.84$\pm$1.4ng/$m\ell$) of nonpregnant cows. The cows of artificial insemination from 61 to 90 days after parturition showed higher progesterone levels. 9. During 20 to 25 days after artificial insemination, the accuracy of pregnancy diagnosis from milk progesterone levels were 94.4% for nonpregnant cows(<2.3ng/$m\ell$), and 75.0% for pregnant cows( 3.2ng/$m\ell$). The average overall accuracy of pregnancy prediction for nonpregnant and pregnant cows 83.3% 10. The results obtained this study suggest that the understanding of the endocrinological mechanisms by means of milk hormone analysis during the estrous cycle, pregnancy and parturition would give the basic information needed for increasing efficiency of reproduction. This study would not only provide an accurate method of the early pregnancy diagnosis by milk progesterone levels but also contribute to the research of providing the method of detecting of FSH levels in milk, which was difficult in blood serum.

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동자개 (Pseudobagrus fulvidraco)의 난모세포 성숙과 배란에 대한 스테로이드와 HCG의 in vitro효과 (Effects of Steroids and HCG on in vitro Maturation and Ovulation of Oocyte in Banded Catfish, Pseudobagrus fulvidraco)

  • 임상구;백혜자;한창희
    • 한국수산과학회지
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    • 제30권2호
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    • pp.203-210
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    • 1997
  • 동자개 난모세포의 성숙과 배란에 있어 스테로이드와 HCG(human chorionic gonadotropin)의 효과에 대한 실험이 in vitro에서 이루어졌으며, 난모세포들은 $17\alpha,\;20\alpha-dihydroxy4-pregnen-3-one\;(17\alpha\;20\alpha\;OHP),\;17\alpha-hydroxyprogesterone\;(17\alpha\;OHP),\;progesterone\;(P_4),\;estradiol-17{\alpha}E_2)$ 과 HCG가 첨가된 Leibovitz L15 배지에서 성숙되어졌다. 60시간 배양후에 난모세포의 성숙능력은 난핵포붕괴(germinal vesicle breakdown, GVBD) 비율에 의해 평가되었다. GVBD 비율은 $17\alpha\;20\alpha\;OHP,\;17\alpha\;OHP,\;P_4$ 그리고 HCG의 첨가에 의해 유의하게 (P<0.05) 증가하였으며, 그 중 $17\alpha\;20\alpha\;OHP$ HCG에서 가장 높은 GVBD 비율을 보였다. 난모세포들 $17\alpha\;20\alpha\;OHP,\;17\alpha\;OHP,\;P_4$$10\~1,000ng/ml$포함된 배지에서 16시간 배양한 결과, $17\alpha\;20\alpha\;OHP\;10\~100ng/m1(65\%)$의 GVBD 비율은 $17\alpha\;20\alpha\;OHP(40\%)$$P_4(35\%)$에서 보다 나은 효과를 보였다. GVBD유도에 대한 효과는 $17\alpha\;20\alpha\;OHP$에서 $10\~100\;ng/ml$배지, HCG를 첨가하여 60시간 배양한 배란유도 실험에서 $17\alpha\;20\alpha\;OHP\;10\~100ng/ml$에서, HCG는 $50\~500IU/ml$의 배지에서 배란율이 유의하게 증가하였다. 그러나 $17\alpha\;20\alpha\;OHP\;1,000ng/m1$와 HCG 5IU/ml의 배지에서는 대조구의 배란율과 차이를 보이지 않았다. 이러한 결과로 $E_2$를 제외한 스테로이드와 HCG는 동자개의 난모세포 성숙과 배란을 in vitro에서 유도할 수 있으며, $17\alpha\;20\alpha\;OHP$와 HCG는 다른 스테로이드에 비해 높은 율의 난모세포 성숙과 배란을 유도하였다.

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Effects of Essential Fatty Acids during In Vitro Maturation of Porcine Oocytes: Hormone Synthesis and Embryonic Developmental Potential

  • Kim, Kang-Sig;Park, Hum-Dai
    • 한국동물생명공학회지
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    • 제34권2호
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    • pp.75-85
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    • 2019
  • Omega-3 α-linolenic acid and omega-6 linoleic acid are essential fatty acids for health maintenance of human and animals because they are not synthesized in vivo. The purpose of this study was to evaluate the effect of α-linolenic acid and linoleic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of α-linolenic acid and linoleic acid were added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, oocyte nuclear-maturation rate, blastocyst rate, blastocyst quality, and levels of prostaglandin E2, 17β-estradiol, and progesterone in the spent medium. High doses (100 μM) of α-linolenic acid and linoleic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 μM α-linolenic acid and 10 μM linoleic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17β-estradiol / progesterone also significantly increased compared with control group (3.59 ± 0.22 vs. 2.97 ± 0.22, 3.4 ± 0.28 vs. 2.81 ± 0.19, respectively; p < 0.05). Our results indicated that supplementation with appropriate levels of α-linolenic acid and linoleic acid beneficially affects the change of hormone synthesis (in particular, an appropriate increase in the 17β-estradiol / progesterone synthesis ratio) for controlling oocyte maturation, leading to improved embryo quality. However, high doses of α-linolenic acid and linoleic acid treatment results in detrimental effects.