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Motion Compensation and B Picture

  • 전병문
    • Broadcasting and Media Magazine
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    • v.7 no.3
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    • pp.19-23
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    • 2002
  • 본 장에서는 H.26L 비디오 코덱에 적용되는 움직임 보상 기법(motion compensation) 및 B 픽쳐(Bi-predictive picture) 에 대해 다루기로 한다. 먼저 H.26L 비디오 코덱이 H.263 또는 MPEG-4 Part 2와 같은 종래 비디오 코덱들과 움직임 보상 관점에서 다른 점을 살펴보면, 복수개의 레퍼런스 픽쳐(multiple reference picture) 로부터 움직임 추정 (motion estimation) 밀 움직임 보상을 수행하고, 16x16 매크로 블록 크기부터 16x8, 8x16, 8x8블록 크기에서 매크로 블록 모드 타입이 결정되고 8x8 모드는 다시 8x4, 4x8, 4x4 단위에서 서브 모드 타입이 결정된다. 따라서 한 개의 매크로블록은 최대 16개의 모션벡터를 깆을 수 있다. 또한 복수개의 레퍼런스 픽쳐와 다양한 블록 타입을 적용함에 따라 모션벡터 예측(PMV: prediction of motion vector) 은 현재 블록과 이웃하는 블록들 사이의 레퍼런스 픽쳐 인덱스 비교 및 현재 블록 타입이 16x8또는 8x16 일 때 방향성 예측을 허용하는 새로운 기법들이 소개되어 있다. 그리고 코딩 효율을 높이기 위한 방법으로서 1/4 pel 단위의 움직임 보상을 하여 블록의 예측 정확도를 높이도록 하고 있다. 한편, H.26L에서의 B 픽쳐는 종래 비디오 코덱에 비해 확장된 정의를 갖고 있다. 예를 들어, 종래의 비디오 코덱에서는 B 픽쳐가 시간 스케일러빌러티(temporal scalability)에 사용됨에 따라 레퍼런스 픽쳐로서 사용될 수 없지만, H.26L은 B 픽쳐가 레퍼런스 픽쳐로서 사용되는 것을 허용하고 있다. 또한 종래의 B 픽쳐가 양방향에서 움직임 보상을 한 것과 달리 H.26L은 동일 방향에 존재하는 두개의 레퍼런스 픽쳐에서도 움직임 보상을 허용한다.

Development of a Novel Long-Range 16S rRNA Universal Primer Set for Metagenomic Analysis of Gastrointestinal Microbiota in Newborn Infants

  • Ku, Hye-Jin;Lee, Ju-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.24 no.6
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    • pp.812-822
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    • 2014
  • Metagenomic analysis of the human intestinal microbiota has extended our understanding of the role of these bacteria in improving human intestinal health; however, a number of reports have shown that current total fecal DNA extraction methods and 16S rRNA universal primer sets could affect the species coverage and resolution of these analyses. Here, we improved the extraction method for total DNA from human fecal samples by optimization of the lysis buffer, boiling time (10 min), and bead-beating time (0 min). In addition, we developed a new long-range 16S rRNA universal PCR primer set targeting the V6 to V9 regions with a 580 bp DNA product length. This new 16S rRNA primer set was evaluated by comparison with two previously developed 16S rRNA universal primer sets and showed high species coverage and resolution. The optimized total fecal DNA extraction method and newly designed long-range 16S rRNA universal primer set will be useful for the highly accurate metagenomic analysis of adult and infant intestinal microbiota with minimization of any bias.

Genetic Diversity and Molecular Phylogeny of Cyanobacteria from Sri Lanka Based on 16S rRNA Gene

  • Wanigatunge, R.P.;Magana-Arachchi, D.N.;Chandrasekharan, N.V.;Kulasooriya, S.A.
    • Environmental Engineering Research
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    • v.19 no.4
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    • pp.317-329
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    • 2014
  • The diversity of cyanobacteria in Sri Lanka was studied in different water reservoirs, paddy fields, brackish water and tsunami affected areas using light microcopy, 16S rRNA sequences, followed by phylogenetic analysis. Based on light microscopy, 24 genera were identified from environmental samples belonging to the orders Chroococcales, Oscillatoriales, Pleurocapsales and Nostocales. In cultures, 33 genera were identified from all five cyanobacterial orders, including Stigonematales. Based on 16S rRNA gene sequences and their morphology, two isolates were identified up to species level, 72 to genus level, one isolate up to family and 11 up to order level. Twelve isolates couldn't be assigned to any taxonomic level. The results of 16S rRNA gene sequences along with the phylogenetic analysis indicated that some cyanobacterial isolates could be accommodated to genus or order level. The 16S rRNA sequence analysis data in this study confirmed that order Nostocales and order Pleurocapsales cyanobacteria are monophyletic while orders Chroococcales, Oscillatoriales and Stigonematales cyanobacteria are polyphyletic. Polyphasic approach including the combination of light microscopy, cultures and the analysis of 16S rRNA gene sequences provide a promising approach to ascertain the diversity of cyanobacteria in different habitats.

AMEX: Extending Addressing Mode of 16-bit Thumb Instruction Set Architecture (AMEX: 16비트 Thumb 명령어 집합 구조의 주소 지정 방식 확장)

  • Kim, Dae-Hwan
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.11
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    • pp.1-10
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    • 2012
  • In this paper, the extension of the addressing mode in the 16-bit Thumb instruction set architecture is proposed to improve the performance of 16-bit Thumb code. The key idea of the proposed approach is the introduction of new addressing modes for more frequent instructions by using the saved bits from the reduction of the register fields in less frequently used instructions. The proposed approach adopts efficient addressing modes from the 32-bit ARM architecture, which is the superset of the 16-bit Thumb architecture. To speed up access to a data list, scaled register offset addressing mode and post-indexed addressing mode are introduced for load and store instructions. Experiments show that the proposed approach improves performance by an average of 8.5% when compared to the conventional approach.

Performance Comparisons on the Strategies of Sleep Window Size Adjustment for Power Saving in IEEE 802.16e (IEEE 802.16e에서 전력 절약을 위한 수면 윈도우 크기 조정 방법들의 성능 비교)

  • Son, Dae-Hong;Roh, Byeong-Hee
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.11A
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    • pp.1113-1119
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    • 2007
  • In this paper, first, we proposed various possible candidate schemes for determining initial sleep window size and varying sleep window size in sleep mode for power saving class I of IEEE 802.16e. Then, we analyzed and compared the power saving performances of those schemes. The schemes used for the comparisons are EICV of IEEE 802.16e, and other comparable schemes such as EIED, EILD, LIED and LILD. Experimental results show that LILD and EIED outperform other comparable schemes. Especially, EIED shows the better performances for the case of high traffic intensity environments, while LILD does for the low traffic intensity case.

Identification and Comparison of the Nucleotide Sequence of 16S-23S rRNA Gene Intergenic Small SR(Spacer Region) of Lactobacillus rhamnosus ATCC 53103 with Those of L. casei, L. acidophilus and L. helveticus

  • Byun, J.R.;Yoon, Y.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1816-1821
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    • 2003
  • Reliable PCR based identification of lactobacilli has been described utilizing the sequence of 16S-23S rRNA intergenic spacer region. Those sequence comparisons showed a high degree of difference in homology among the strains of L. rhamnosus, L. casei, L. acidophilus and L. helveticus whose 16S-23S rRNA intergenic small SR's sizes were 222 bp, 222 bp, 206 bp and 216 bp respectively. The sequence of 16S-23S rRNA intergenic spacer region of L. rhamnosus ATCC 53103 revealed the close relatedness to those of L. casei strains by the homology ranges from 95.4% to 97.2%. 16S-23S rRNA intergenic spacer region nucleotide sequence of L. acidophilus showed some distant relatedness with L. rhamnosus ATCC 53103 with the homology ranges from 40.3% to 41.8% and that with L. helveticus was shown to be 30% of homology, which exists at the most distant phylogenetic relatedness. The identification of species and strain of lactobacilli was possible on the basis of these results. The common sequences among the 17 strains were CTAAGGAA located in the initiating position of the DNA and some discrepancies were found between the same strains based on these results.

Effect of Photoperiodic Regimes on Oviposition and Colony Development of the Bumblebee, Bombus ignitus

  • Yoon, Hyung-Joo;Kim, Sam-Eun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.2
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    • pp.151-154
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    • 2003
  • The effect of photoperiodic regimes on the oviposition and colony development of Bombus ignitus queens was examined with 0L, 8L, and 16L under $27^{\circ}C$ and 65% R. H. Among these photoperiod regimes, the oviposition rate at 8L and 16L was 80.2% and 83.1%, respectively, which was 12-15% higher than that at the dark condition (0L). Duration up to first oviposition at 8L and 16L was 17.5 days and 16.5 days, respectively, which was 2-3 days shorter than that at 0L. The colony foundation rate at 8L and 16L was 9.2% and 10.4%, respectively, which corresponded to 1.7-2.0-fold higher than the value at 0L. In addition, the rate of progeny-queen production at 8L and 16L was also two fold higher than that at 0L. Taken these together, the light conditions (8L and 16L) rather than dark condition (0L) were more suitable for oviposition and colony development for B. ignitus in the indoor rearing condition.

Species Identification of Five Penaeid Shrimps Using PCR-RFLP and SSCP Analyses of 16S Ribosomal DNA

  • Khamnamtong, Bavornlak;Klinbunga, Sirawut;Menasveta, Piamsak
    • BMB Reports
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    • v.38 no.4
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    • pp.491-499
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    • 2005
  • DNA-based molecular markers for differentiation of five penaeid shrimps (Penaeus monodon, P. semisulcatus, Feneropenaeus merguiensis, Litopenaeus vannamei and Marsupenaeus japonicus) were developed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-stranded conformation polymorphism (SSCP) of 16S ribosomal (r) DNA. Differentiation of P. monodon, P. semisulcatus and L. vannamei can be unambiguously carried out by PCR-RFLP of 16S $rDNA_{560}$ whereas P. semisulcatus and M. japonicus shared a BABB mitotype. These shrimps were successfully discriminated by SSCP analysis of 16S $rDNA_{560}$. Nevertheless, the amplification success for L. vannamei and F. merguiensis was not consistent when tested against larger sample sizes. As a result, 16S $rDNA_{560}$ of an individual representing the most common mitotype of each species was cloned and sequenced. The new primer pair was designed and tested against the large sample sizes (312 bp product, N = 185). The amplification success was consistent across all species. PCR-RFLP of 16S $rDNA_{312}$ was as effective as that of 16S $rDNA_{560}$. Differentiation of all shrimp species were successfully carried out by SSCP analysis.

Comparison of p16INK4a Immunocytochemistry with the HPV Polymerase Chain Reaction in Predicting High Grade Cervical Squamous Intraepithelial Lesions

  • Indarti, Junita;Fernando, Darrell
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.9
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    • pp.4989-4992
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    • 2013
  • Aim: To compare p16INK4a immunocytochemistry with the HPV polymerase chain reaction in predicting high grade cervical squamous intraepithelial lesions. Materials and Methods: This diagnostic case-control study was conducted from January 2010 until December 2010. We obtained 30 samples, classified according to the degree of cervical intraepithelial neoplasia (CIN): 11 samples for CIN 1, 9 samples for CIN 2, and 10 samples for CIN 3. HPV PCR, p16INK4a immunocytochemistry, and histopathological examination were performed on all samples. Statistical analysis was conducted using SPSS 20.0. Results: In predicting CIN 2-3, we found p16INK4a to have similar specificity and positive predictive value as HPV PCR (95%, 97.2% vs 96.7%), but better sensitivity (87.5% vs 72.5%) and negative predictive value (82.1% vs 67.6%). The most prevalent types of high-risk HPV in our study were HPV 33, 35, 58, 52, and 16. Conclusions: p16INK4a has better diagnostic values than HPV PCR and may be incorporated in the triage of ASCUS and LSIL to replace HPV PCR. Genotype distribution of HPV differs in each region, providing a challenge to develop HPV vaccines based on the epidemiology of HPV in that particular region.

A Study on Performance Improvement of Convolution coded 16 QAM Signal Reception with Maximum ratio combining Diversity in Fading Channel (페이딩 채널에서 선택 합성 다이버시티 기법과 길쌈 부호화 기법을 채용한 16 QAM 신호의 수신 성능 개선에 관한 연구)

  • Lee, Ho-Young;Kim, Eon-Gon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2008.10a
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    • pp.467-472
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    • 2008
  • In this paper, we analyzed the error rate Performance of convolution coded 16 QAM signal with Optimum Threshold Detection with selective combining diversity in Rician Fading Environments. The performance of 16-QAM signal with CTD (conventional threshold detection) which employs convolution coding technique was analyzed and the performance improvement of convolution coded 16-QAM signal with OTD (optimum threshold detection) which is varied according to fading parameter "K" and AWGN in Rician Fading channel was simulated. As a result of analysis, it was shown the effect of performance improvement to overcome the environment of mobile radio data communication channel.

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