• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1235-1243
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• 2020

The use of microalgal biomass as feedstock for biofuels has been discussed for decades as it provides a sustainable approach to producing fuels for the future. Nonetheless, its feasibility has not been established yet and various aspects of biomass applications such as CO₂ biofixation should also be explored. Therefore, in this study, the CO₂ biofixation and lipid/carbohydrate production potential of Chlorella sp. ABC-001 were examined under various nitrogen concentrations. The highest biomass productivity and CO₂ biofixation rate of 0.422 g/l/d and 0.683 g/l/d, respectively, were achieved under a nitrogen-rich condition (15 mM nitrate). Carbohydrate content was generally proportional to initial nitrate concentration and showed the highest value of 41.5% with 15 mM. However, lipid content showed an inverse relationship with nitrogen supplementation and showed the highest value of 47.4% with 2.5 mM. In consideration as feedstock for biofuels (bioethanol, biodiesel, and biogas), the sum of carbohydrate and lipid contents were examined and the highest value of 79.6% was achieved under low nitrogen condition (2.5 mM). For lipid-based biofuel production, low nitrogen supplementation should be pursued. However, considering the lower feasibility of biodiesel, pursuing CO₂ biofixation and the production of carbohydrate-based fuels under nitrogen-rich condition might be more rational. Thus, nitrogen status as a cultivation strategy must be optimized according to the objective, and this was confirmed with the promising alga Chlorella sp. ABC-001.

• 한국작물학회지
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• 제38권5호
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• pp.383-390
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• 1993

본 실험은 1992년 일본 동경농업대학 온실에서 질소 추비와 생장억제제 처리가 벼의 생육과 도복형질에 미치는 형태학적 특성과의 관계를 조사함과 동시에 내성 에틸렌 발생에 미치는 영향을 구명하기 위하여 실험을 수행한바 아래와 같은 결과를 얻었다. 1. 식물생장억제제인 Inabenfide는 초장은 감소시켰지만 수량구성요소, 엽록소 함량, $CO_2 동화량 및 분벽에는 변화가 없었다. 2. Inabenfide를 수잉기에 처리하였을 때 절간 신장을 감소시켰으나, 세 번째와 네 번째의 절간 직경과 줄기의 두께를 증가시켰다. 3. 질소추비는 대체로 초장과 $CO_2 동화률에는 뚜렷하게 영향을 나타내지 않았다. 4. 에틸렌 발생은 생장억제제에 대하여 많은 변화를 보여 주었으며 Inabenfide를 출수 15일 전에 처리하였을 때 에틸렌 발생은 무처리구보다 낮게 나타났다. 5. 질소 추비량에 따른 에틸렌 발생은 추비량이 많을 때 발생량이 높았으며 질소 추비와 에틸렌발생 사이에는 높은 상관관계가 있는 것으로 나타났다.

• 한국항공우주학회지
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• 제46권6호
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• pp.513-518
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• 2018

KUH-1에 적용되는 질소충전트레일러는 항공기의 주/전륜 완충기, 테일범퍼완충기, 브레이크 축압기, 타이어 등에 질소를 충전하는 장비이다. 명칭에서 알 수 있는 것과 같이 질소충전트레일러는 질소 충전과 더불어 이동성을 가지고 있는 장비이다. 기존 미국의 Tronair사에서 독점 공급하여 국내에서는 수입에 의존하던 품목이며, 본 국산화 개발품은 도입품의 성능을 만족할 뿐만 아니라 운용 시 도입품보다 용이하도록 설계를 추진하였다. 제15-4차 민관공동투자사업으로 선정되어 개발이 진행되었으며, 민관투자기업인 한국항공우주산업과 국방기술품질원의 개발관리 지원하에 개발요구도 분석 및 시험평가가 수행되었으며, 2017년 5월 군사용 적합 판정 결과 승인을 받았다.

• 한국전기전자재료학회논문지
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• 제15권7호
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• pp.576-582
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• 2002

Nonvolatile semiconductor memory devices with reoxidized nitrided oxide(RONO) gate dielectrics were fabricated, and nitrogen distribution and bonding species which contribute to memory characteristics were analyzed. Also, memory characteristics of devices depending on the anneal temperatures were investigated. The devices were fabricated by retrograde twin well CMOS processes with $0.35\mu m$ design rule. The processes could be simple by in-situ process in growing dielectric. The nitrogen distribution and bonding states of gate dielectrics were investigated by Dynamic Secondary Ion Mass Spectrometry(D-SIMS), Time-of-Flight Secondary Ion Mass Spectrometry(ToF-SIMS), and X-ray Photoelectron Spectroscopy(XPS). As the nitridation temperature increased, nitrogen concentration increased linearly, and more time was required to form the same reoxidized layer thickness. ToF-SIMS results showed that SiON species were detected at the initial oxide interface which had formed after NO annealing and $Si_2NO$ species within the reoxidized layer formed after reoxidation. As the anneal temperatures increased, the device showed worse retention and degradation properties. It could be said that nitrogen concentration near initial interface is limited to a certain quantity, so the excess nitrogen is redistributed within reoxidized layer and contribute to electron trap generation.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.198-203
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• 2003

The effects of salmon carcasses on forest and stream ecosystems were determined by nitrogen stable isotope analysis in natural streams in Hokkaido, Northern Japan, where numerous chum salmon (Oncoryhncus keta) were migrated upstream ITom ocean to spawn in autumn. The leaves and soils surrounding riparian forest and stream dwelling invertebrates were collected before and after migration. The nitrogen stable isotope ratio $({\delta}^{15}N)$ of riparian vegetation (Salix spp.) were different depending on the presence of salmon and distance from the stream. The $({\delta}^{15}N)$ of stream dwelling invertebrates were different between salmon present and absent stream. This difference was tested using the experiment channel by implanting salmon carcasses. The nitrogen stable isotope ratio of epilithic algae and leaf shredding animals were nearly 3 higher in the salmon implanted treatment suggesting that around 20％ of salmon derived nitrogen was uptake either in algae and leaf shredding invertebrates. These results suggest that the salmon carcasses effects not only on stream primary production but also on primary consumers, which decompose leaves fertilized with nitrogen from carcasses.

• 생태와환경
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• 제50권4호
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• pp.459-469
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• 2017

본 연구는 박테리아 탈질법을 이용하여 질산성 질소의 질소 및 산소의 안정동위원소 분석법을 연구하였으며, 시료 농도, 미생물 배양시간에 따른 분석값의 변화에 대하여 고찰하였다. 탈질미생물법을 이용하여 시료 내 질산염 농도가 $0.1mg\;L^{-1}$까지 질산성 질소의 산소 및 질소 동위원소 분석이 가능하였고, 0.2‰까지 정확도를 확보하였다. 환경시료(지하수) 내 질산염의 기원을 추적하기 위하여 잠재적 질소 오염원(합성비료, 축산비료)의 동위원소비를 조사한 결과, 합성비료(${\delta}^{15}N-NO_3$ -5~10‰, ${\delta}^{18}O-NO_3$ 0~15‰)는 축산비료(${\delta}^{15}N-NO_3$ 10~23‰, ${\delta}^{18}O-NO_3$ 0~20‰)와 동위원소비가 현격한 차이를 보였다. 연구지역 지하수 동위원소비와 비교한 결과, 계절별로 서로 다른 질소 오염원의 영향을 받는 것으로 여겨진다. 과거 질산염의 안정동위원소를 분석하기 위해서 다양한 방법이 시도되었다. Revesz et al. (1997)은 양이온 교환 수지를 이용한 분리법을 보고하였으며, Silva et al. (2000)와 Fukada et al. (2003)은 음이온 교환 수지를 이용한 분리법을 보고하였고, McIlvin and Altabet (2005)는 카드뮴을 이용한 화학적 변환 방법을 보고하였다. 하지만 이러한 방법에 사용되는 시약은 독성이 강하고 복잡한 전처리 과정으로 인한 긴 전처리 시간을 소요함으로 인하여 분석비용이 비싸다는 단점이 있었다. 하지만 탈질미생물법은 소량의 질산염을 이용하기 때문에 기존 방법에 비해 분석에 사용되는 시료의 부피를 1/100까지 감소시켜 과거 분석이 어려웠던 빙하, 공극수, 해수 등에 대한 분석이 가능한 장점이 있다. 수생태계로 유입되는 다양한 질소 기원을 파악하기 위하여 탈질미생물법으로 분석된 안정동위원소비를 활용한다면 효율적인 수질 관리를 위한 해석기능을 제공할 것이다. 또한, 국내 최초로 지하수 환경시료에 적용함으로써 오염 기원 추적 기법을 확립하는 데 목적을 두고 있으며, 추후 정립된 분석기법을 토대로 환경분야에서 질산성 질소의 오염 인자 판별 연구에 널리 활용되기를 기대한다.

• 환경위생공학
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• 제16권3호
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• pp.42-53
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• 2001

This study were conducted to estimate property of nitrogen dioxide concentration at industrial areas in Korea. The nitrogen dioxide concentrations were measured for 50 dwellings at Ulsan and Yeochon, respectively using the nitrogen dioxide filter badge forming the passive sampler from summer time 1998 to winter time 1999. The major results obtained from this study were as follows: 1. The mean indoor, outdoor, and personal concentrations of nitrogen dioxide during summer time 1998 in Ulsan were 28.9 ppb, 24.8 ppb, 24.8 pub, respectively. 2. The mean indoor, outdoor, and personal concentrations of nitrogen dioxide during winter time 1999 in Yeochon were 11.4 ppb, 12.5 ppb, 15.5 ppb, respectively. 3. The mean concentration of nitrogen dioxide during winter time 1998 were higher than the corresponding levels during summer time 1997 in Ulsan. 4. The mean concentration of nitrogen dioxide in Ulsan were in general higher than the corresponding levels in Yeochon. 5. This results in slightly positive correlation among indoor, outdoor, and personal nitrogen dioxide concentrations for summer time in Ulsan, then it statistical significance is showed(p <.05). These results suggest that nitrogen dioxide concentrations in industrial areas seem to be affected by traffic density and conditions of industrialization.

• 농업과학연구
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• 제14권1호
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• pp.1-15
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• 1987

본(本) 실험(實驗)은 대두(大豆)에 대한 질소(窒素)의 추비효과(追肥效果)를 구명(究明)하고자 1986년(年)에 장엽(長葉)콩을 공시(供試)하여 파종기(播種期)(단작(單作)- 5월(月) 15일(日) 파종(播種), 맥후작(麥後作)- 6월(月) 18일(日) 파종(播種))를 주구(主區), 추비량(追肥量)(0, 3, 6, 9 kg/10 a)을 세구(細區), 추비시기(追肥時期)(배토기(培土期), 개화기(開花期))를 세세구(細細區)로 하여 포장실험(圃場實驗)을 실시(實施)하였던 바 그 얻어진 결과(結果)를 요약하면 다음과 같다. 1. 개화(開花) 및 성숙일수(成熟日數)는 배토기(培土期) 추비구(追肥區)가 개화기추비구(開花期追肥區)보다 1일간(日間) 지연(遲延) 되었다. 2. 주경절수(主莖節數)와 절간장(節間長)은 처리간(處理間)에 변이폭(變異幅)이 작고 일정(一定)한 경향(傾向)이 나타나지 않아서 유의성(有意性)이 인정(認定)되지 않았으나 분지절수(分枝節數)는 맥후작(麥後作)에 비(比)하여 단작(單作)에서 많았으며 두 파종기(播種期) 공히 추비량(追肥量)이 증가할수록 개화기(開花期) 추비구(追肥區)에서 보다 배토기추비구(培土期追肥區)에서 그 경향(傾向)이 현저(顯著)하였는데 특히 10 a 당(當) 6 kg 추비구(追肥區)의 분지절수(分枝節數)가 가장 많아서 고도(高度)의 유의성(有意性)이 인정(認定)되었다. 3. 개화(開花) 10, 25 및 40 일후(日後)의 지상부(地上部) 생체중(生體重)과 건물중(乾物重)은 두 파종기(播種期)에 관계(關係)없이 추비량(追肥量)이 많을수록 무거워서 높은 유의성(有意性)이 인정(認定)되었는데 같은 파종기(播種期)에 있어서는 개화기(開花期) 추비구(追肥區)에서 보다 배토기(培土期) 추비구(追肥區)에서 그 경향(傾向)이 더욱 뚜렷하였다. 4. 근류수(根瘤數) 및 근류생(根瘤生) 건물중(乾物重)은 단작(單作)보다 맥후작(麥後作)에서 현저(顯著)히 감소(減少)하였고 파종기(播種期)에 관계(關係)없이 무추비구(無追肥區)에 비(比)하여 질소추비량(窒素追肥量)이 많을수록 감소(減少)되는 경향(傾向)이었으며 개화기(開花期) 추비구(追肥區)보다 배토기(培土期) 추비구(追肥區)에서 그 감소(減少) 경향(傾向)이 더욱 현저(顯著)하여 고도(高度)의 유의성(有意性)이 인정(認定)되었다. 5. 개체당(個體當) 내수(萊數), 입수(粒數) 및 자실중(子實重)은 맥후작(麥後作)보다 단작(單作)에서 많았고 질소(窒素) 추비량(追肥量)이 많을수록 현저(顯著)히 증가하는 경향(傾向)이었는데, 개화기(開花期) 추비구(追肥區)보다 배토기(培土期) 추비구(追肥區)에서 많았고 특히 배토기(培土期)의 10 a 당(當) 6 kg 추비구(追肥區)에서 가장 높았다. 또한 10 a 당(當) 수량(收量)도 처리간(處理間)에 높은 유의성(有意性)이 인정(認定)되었는데 파종기(播種期)에 관계(關係)없이 배토기(培土期)의 10 a 당(當) 6 kg 추비구(追肥區)에서 가장 많았다.

• Journal of Animal Science and Technology
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• 제47권3호
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• pp.481-490
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• 2005

Nitrate contamination in water system is a critical environmental problem caused by excessive application of chemical fertilizer and concentration of livestock. In order to prevent further contamination, therefore, it is necessary to understand the origin of nitrate in nitrogen loading sources and manage the very source of contamination. The objective of this study was to examine the nitrate contamination sources in different agricultural system by using nitrogen isotope ratios. Groundwater and runoff water samples were collected on a monthly basis from February 2003 to November 2003 and analyzed for nitrogen isotopes. The nitrate concentrations of groundwater in livestock fanning area were higher than those in conventional and organic fanning area and exceeded the national drinking water standard of 10mg N/ l. The ${\delta}^{15}N$ranges of chemical fertilizer and animal manure were - 3.7${\sim}$+2.3$\textperthousand$ and +12.5${\sim}$26.7$\textperthousand$, respectively. The higher ${\delta}^{15}N$ of animal manure than those of chemical fertilizer reflected isotope fractionation and volatilization of '''N. The different agricultural systems and corresponding average nitrate concentrations and ${\delta}^{15}N$ values were: conventional farming, 5.47mg/e, 8.3$\textperthousand$; organic fanning, 5.88mg/e, 10.1$\textperthousand$; crop-livestock farming, 12.5mg/e, 17.7%0. These data indicated that whether conventional or organic agriculture effected groundwater and runoff water quality. In conclusions, relationship between nitrate concentrations and ${\delta}^{15}N$ value could be used to make a distinction between nitrate derived from chemical fertilizer and from animal manure. Additional investigation is required to monitor long-term impact on water quality in accordance with agricultural systems.

• Asian-Australasian Journal of Animal Sciences
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• 제11권6호
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• pp.732-738
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• 1998

Three crossbred gilts weighing $61{\pm}2kg$ ($mean{\pm}SD$) and three gilts weighing $52{\pm}3kg $ on the day before the first treatment began (d -1) were used for each of two experiments (Exp. 1 and Exp. 2), respectively. In Exp. 1, all pigs were fed the experimental diet (CP 19%) from d -7 to the end of study (d 21) to verify that nitrogen retention is constant during the 21 -d period. In Exp. 2, pigs were fed the control diet (CP 15.5 %) from d -7 to d 8 and then the low-lysine diet from d 9 to d 16 in order to determine how rapidly dietary changes in amino acid composition results in a new equilibrium for nitrogen metabolism. The amount of urine nitrogen loss was not different over 21 days (p > 0.10). Rates of nitrogen retention were not different among pigs (p > 0.10) nor over time (p > 0.10). Average nitrogen retention during the period was 1.00 g/kg $BW^{0.75}$ per day. The apparent biological value was 41%, which did not change over the 3-week period (p > 0.10). The overall efficiency of nitrogen use for nitrogen retention was 35% (Exp. 1). The amount of nitrogen loss in urine and the efficiency of nitrogen utilization for nitrogen gain reached a new equilibrium within 2 to 3 d after the diet was changed. The low-lysine diet resulted in a 20% increase of nitrogen loss in urine (p < 0.001) and a 9% decline in efficiency of nitrogen use for nitrogen retention (p < 0.001). Nitrogen retention while the pigs were fed the control diet was also higher than the retention when pigs were fed the low lysine diet (p < 0.001). The efficiency of nitrogen use for nitrogen retention in pigs fed the control diet was 57% (Exp. 2), which was higher (p < 0.001) than that from pigs fed the low-lysine diets (52%).