• 생명과학회지
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• 제23권8호
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• pp.1010-1018
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• 2013

본 연구에서는 온실가루이 방제를 위하여 감염된 이병체로부터 균주 분리 및 농촌진흥청 및 경상북도농업기술원에서 균주를 분양 받아 5 종의 균주를 확보하여, 최종적으로 활성이 가장 높은 분리균주 M130을 선발하였다. Beauveria bassiana M130을 이용하여 균주가 갖는 효소학적 활성을 분석하였다. Chitinase와 protease의 활성을 조사한 결과, 타 균주에 비하여 높은 효소학적 활성을 가지는 조건을 얻었으며, 선발된 균주의 포자생산의 최적화를 위한 방안으로 PDB (potato dextrose broth)배지와 미강추출물을 이용한 ERBM (extracted rice bran medium)을 비교하여, 더 높은 생육도와 포자 생성량을 보인 ERBM을 본 균주를 배양하는 기본배지로 선발하였다. ERBM을 이용한 액체배양시의 최적조건으로, 기본배지인 ERBM에 0.5% $(NH_4)_2SO_4$를 첨가하여 pH 5, $28^{\circ}C$에서 배양최적화를 하였다. 향후 곤충병원성곰팡이 Beauveria bassiana M130의 최적화 조건에 따라 배양을 한다면 포자의 생산량 및 방제효과가 높아질 것으로 사료된다.

• 한국식품영양학회지
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• 제29권5호
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• pp.785-794
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• 2016

This research was conducted to evaluate quality changes in traditional Doenjang and manufactured Doenjang during a storage period of 8 weeks. Low-salt Doenjang and commercial Doenjang were purchased from different manufacturers and proximate analysis as well as changes in isoflavone, polyphenol, flavonoid contents of the samples were investigated using a mass spectrophotometer. The salinity of traditional Doenjang, low salt Doenjang, and commercial Doenjang were $13.2{\pm}1.15$, $7.17{\pm}2.74$, $10.67{\pm}0.35%$, respectively and the salt concentrations of the soybean pastes did not change during storage. After 8 weeks at $35^{\circ}C$, chromatic values of all the paste samples decreased somewhat, with traditional Doenjang exhibiting fewer changes as compared to manufactured Doenjang. Amino acid nitrogen, acidity, microbial population all tended to increase with time, although some samples showed fluctuations during the test period. Moreover, the total isoflavone contents of traditional Doenjang increased with storage time while that of manufactured Doenjang tended to decrease. The isoflavone aglycone was shown to be the highest in traditional Doenjang, while isoflavone glycoside was abundant in manufactured Doenjang. Total flavonoid contents showed similar trends regardless of samples; initial contents of total flavonoid was 0.6 mg/g which increased to more than twice to 1.4 mg/g at the end of storage period. Composition profile of Doenjang extracts was analyzed using UPLC-Q-ToF.

• 한국초지조사료학회지
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• 제7권3호
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• pp.128-134
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• 1987

刈取가 알팔파根瘤의 着生 및 發達과 室素固定活性에 미치는 影響을 檢討하기 위하여 圃場實驗에서는 刈取區와 非刈取區로 구분하여 時期別로 Acetylene還元法에 의하여 根瘤의 室素固定活性을 測定하였다. 養液栽培實驗에서는 地上部의 50%와 90%刈取, 花芽의 除法, 根瘤의 50%와 100% 除法등을 組合處理後 알팔파 再生과 根瘤의 着生과 發達에 미치는 影響을 檢討하였다. 1. 圃場實驗에서 根瘤의 무게는 1回刈取後 30%, 2 回刈取後 25%의 減少가 있었고 2 回刈取後 30日부터는 減少가 없었다. 2. 7月初, 8月初, 9月初에 刈取區의 specific nodule activity(SNA)는 非刈取區보다 높았으며 total nodule activity도 刈取區가 非刈取區보다 높았다. 開花期의 刈取는 根瘤의 室素固定活性 유지에 도움이 되는 것으로 나타났다. 3. 養液栽培實驗에서는 地上部의 50% 刈取는 알팔카의 再生과 根瘤의 發達에 影響이 없었으나 地上部의 90% 刈取는 再生을 느리게 하고 根瘤의 脫落을 심하게 조장하고 着生된 根瘤나 새로이 着生된 根瘤의 發達을 나쁘게 한 것으로 보아 알팔파根瘤의 室素固定活性을 높은 狀態로 維持하기 위해서는 너무 낮은 刈取를 피하는 것이 바람직할 것으로 보여진다.

• 한국토양비료학회지
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• 제19권1호
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• pp.38-49
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• 1986

우리나라 대두작부형별(大豆作付型別)로 토착근류균(土着根瘤菌)의 분포상태(分布狀態)와 토양(土壤)의 이화학적특성(理化學的特性) 및 공존미생물(共存微生物)과의 상호관계(相互關係)에 관(關)하여 조사(調査) 비교(比較)한 결과(結果)는 다음과 같다. 1. 전국(全國) 60개(個) 밭 토양(土壤)의 토착근류균(土着根瘤菌)의 분포(分布)는 $2{\sim}197{\times}10^4cells/g$ 범위(範圍)였으며, 대두작부간(大豆作付間) 평균(平均) 토착근류균수(土着根瘤菌數)는 대두무재배지(大豆無栽培地)는 $20.5{\times}10^4cells/g$, 대두재배지(大豆栽培地)에서는 $35.6{\times}10^4cells/g$로서 대두무재배지(大豆無栽培地)보다 높은 균수밀도(菌數密度)를 보였다. 이들 토착근류균수(土着根瘤菌數)를 토성별(土性別)로 보면 양토(壤土)>사토(砂土)>사양토(砂壤土)>식양토(埴壤土)>식토(埴土) 순위(順位)로 많았으며, 9개(個) 농업기후대권간(農業氣候帶圈間)에는 경기만권(京畿彎圈)이 $10{\sim}15{\times}10^4cells/g$로 제일 적었고, 남부권(南部圈)이 $71{\sim}145{\times}10^4cells/g$로서 높은 분포밀도(分布密度)를 보였다. 2. 토착근류균수(土着根瘤菌數)는 일반토양미생물(一般土壤微生物)인 호기성총세균(好氣性總細菌) 및 총사상균수(總絲狀菌數)와는 정(正)의 상관관계(相關關係)를 보였으나 총방선균수(總放線菌數)와는 부(負)의 상관관계(相關關係)를 나타내었다. 3. 토착근류균수(土着根瘤菌數)와 토양화학성(土壤化學性)과의 상호관계(相互關係)에서 산도(酸度), 유효인산(有效燐酸), 치환성(置換性) 석회(石灰), 고토(苦土), 동(銅) 및 붕소함량(硼素含量)과는 유의(有意)한 정(正)의 상관관계(相關關係)를 보였으나, 치환성(置換性) 나트리움, 알루미늄, 활성철(活性鐵) 및 황산(黃酸)과는 부(負)의 상관관계(相關關係)를 보였다.

• 한국연초학회지
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• 제15권1호
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• pp.63-74
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• 1993

Changes in the amount of ribulose 1, 5-bisphosphate carboxylase/oygenase(=RuBisCO) protein, namely fraction I protein, and the protease activity were determined in the 10th leaf of tobacco(Nicotiana tabacum, var. Ky-57) from 10 days after emergence through senescence at 5 days interval. The amount of RuBisCO per deveined leaf rapidly increased during the early growing season, reached a maximal quantity at the around 20 days after leaf emergence, when the leaf has gone through its most rapid expansion, and began gradually to decrease till 30 days after leaf emergence, thereafter significantly declined to 45 days that the leaf has been dried up partly. The pattern of the ratio of RuBisCO protein to soluble protein in quantity changed similar to that of RuBisCO contents in a leaf, that was 43%, 60%, and 21% at the around 10 days, 20 days, and 45 days, respectively. And RuBisCO contents was linearly correlated with the concentration of chlorophyll(r=0.98) throughout the life span of the leaf. So, it was assumed that the leaf color can be a useful indicator for judging whether RuBisCO contents higher or not in tobacco leaves without homogenization. On the other hand, the protease activities for degradation of casein were assayed at pH 5.5. 7.0. and 8.5 with crude extracts desalted on Sephadex G-25. The highest caseolytic activity was found at pH 5.5 throughout the life sawn of the leaf. Also, the activity at 5.5 became gradually to increase from 30 days after leaf emergence, when RuBisCO protein had became to disappear and remarkably increased in the last stage of senescence, although nitrogen contents of the leaf had reached low levels. The caseolytic activity at pH 5.5 was in negative correlation with RuBisCO contents throughout the life span of the leaf, but not in lineality between them. In other words, the caseolytic activity increased in a rapid exponential manner when RuBisCO contents had reached some low levels. These results showed that the leaf age, namely harvesting time, is a very important factor for the production of the tobacco leaf containing higher RuBisCO protein. It was concluded that the practical harvesting time is between 20 days and 30 days after the leaf emergence from the present results.

• 대한수의학회지
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• 제15권2호
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• pp.187-198
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• 1975

Although considerable research has been done on the blood chemistry of domestic animals, little work has been made of the changes associated with age. Moreover, the records about physiology of the goat were not much available in Korea, and a comprehensive survey of the blood values of the Korean native goat has not been made. The object of the present investigation was to make good this deficiency and to suggest standards for the blood chemical values of Korean native goats from birth to maturity. The goats were kept under average farming conditions in Korea. Blood samples were collected from the jugular vein at birth, at one and four days, at one, two, three and four weeks, and at two, three, six, nine and twelve months of age. The results obtained in this work were summarized as follows: 1. The highest concentration of serum glucose was observed at birth and it decreased gradually until three months of age, showing a steady state thereafter. 2. The concentration of total serum protein was a little higher at one day of age than at birth and showed a steady state until seven days of age; then it decreased slightly at two weeks of age and recovered at three weeks of age and showed a steady state until twelve months of age. The concentration of serum albumin was increased a little at one day of age and revealed a steady state thereafter. The concentration of serum globulin showed three phases - a fall during the first two weeks to reach minimum, a rise to the fourth week, and a fall at two months of age, showing a steady state thereafter. The lowest value of albumin fraction was observed at birth, then the value increased gradually until three months of age and revealed a stealer state thereafter. The highest value of globulin fraction was observed at birth, then the gradual decrease of the value was seen until three months of age; thereafter the value showed a steady state. 3. Albumin/globulin ratio was the minimum at birth, then it increased to reach a maximum at two weeks of age and decreased a little thereafter. 4. The concentration of total serum cholesterol showed a gradual increase during the first three months and fell to reach adult revel at six months of age. 5. Urea nitrogen in serum decreased during the first week to reach a minimum, then it increased at three months of age, and showed a fell to mature level at six mouths of age. 6. The concentration of serum creatinine was not affected by age. 7. The concentration of total serum calcium was a little higher during the first two weeks than the other period during the first year of life. 8. The concentration of serum inorganic phosphorus showed a gradual rise to reach a maximum and a gradual fall to reach adult level at nine months of age. 9. No significant sex differences of serum chemical values were recognized.

• 한국잡초학회지
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• 제15권1호
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• pp.63-72
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• 1995

제초제(除草劑) Napropamide의 분해능(分解能)이 뛰어난 균주(菌株)를 선발(選拔)하고 그의 분해특성(分解特性)을 구명(究明)할 목적(目的)으로 농약(農藥) 무처리토양(無處理土壤)으로부터 세균(細菌)을 분리(分離)한 후(後) 이들 균(菌)을 Napropamide 함유(含有) NB배지(培地)에 접종(接種)하여 생존력(生存力)과 Napropamide 분해능(分解能)을 조사(調査)하였으며, 분해능(分解能)이 우수(優秀)한 세균(細菌)에 대해 Napropamide를 탄소원(炭素源) 혹은 질소원(窒素源)으로서의 이용여부(利用與否)와 반복처리(反覆處理)에 의한 세균(細菌)의 Napropamide 분해양상(分解樣相)을 조사(調査)하였는 바 그 결과는 다음과 같다. 공시토양(供試土壤)인 식양토(埴壤土)에서 분리(分離)한 세균(細菌)은 그람양성균(陽性菌) 및 그람음성균(陰性園)이 각각(各各) 4속(屬) 10균주(菌株)씩이었고, 이들 세균(細菌)의 Napropamide에 대한 생존력시험(生存力試驗)에서 공시균주(供試菌株) 모두 100ppm에서는 활발(活撥)하게 증식(增植)되었고 1500ppm까지 생존력(生存力)이 뛰어난 균주(菌株)는 Staphylococcus속(屬) 2균주(菌株), Corynebacterium속(屬) III, 기타(其他) 속(屬) II이었다. 공시균주중(供試菌株中) 20%이상(以上) Napropamide 분해능(分解能)을 가진 세균(細菌)은 Staphylococcus속(屬) II와 Actinobacillus속(屬) I이었고, 이들 두 균주(菌株)는 Napropamide를 단일(單一) 질소원(窒素源)으로서는 이용(利用)하지 못하였으나, Staphylococcus속(屬) II가 Napropamide를 단일(單一) 탄소원(炭素源)으로 이용(利用)하는 것으로 나타났다. Napropamide의 반응처리(反覆處理)에 따른 Staphylococcus속(屬) II의 공시약제(供試藥劑)의 분해(分解)는 1회처리(回處理)에서 급속(急速)히 분해(分解)되었고 2회연용처리(回連用處理) 후(後)에는 분해(分解)가 지연(遲延)되었으나 3회연용처리(回連用處理)에서는 다시 급속(急速)한 분해(分解)가 일어났다.

• 한국잡초학회지
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• 제15권1호
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• pp.13-18
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• 1995

담수직파(湛水直播) 논에 발생(發生)되는 괴불을 효율적(效率的)으로 방제(防除)하기 위(爲)해 괴불의 발생(發生) 생태(生態)를 조사(調査)하고 약제(藥劑)를 이용(利用)한 방제(防除) 방법(方法)을 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 괴불의 발생(發生)은 적색광(赤色光)인 660nm광하(光下)에서 발생율(發生率)이 높았고 450nm인 청색광(靑色光)에서는 괴불발생(發生)이 억제(抑制)되었다. 2. 시비량(施肥量)이 적은 처리구(處理區)가 괴불의 발생량(發生量)이 높았고 비료(肥料)와 토양(土壤)의 혼화비율(混和比率)이 낮은 처리구(處理區)에서 괴불의 발생(發生)이 많았다. 3. 카바에이트계(系)와 트리아진계(系) 제초제(除草劑)가 괴불발생(發生) 억제효과(抑制效果)가 높았으나 발생(發生)된 괴불에 대(對)해서는 카바에이트계(系)는 발생(發生) 억제효과(抑制效果)가 없었고 트리아진계(系) 제초제(除草劑)는 발생전(發生前) 및 발생후(發生後)에도 높은 괴불 방제효과(防除效果)를 보였으며 쿠퍼등(等) 살균제(殺菌劑)도 괴불에 대(對)해 방제효과(防除效果)가 높았다. 4. 포장(圃場)에서의 괴볼 방제효과(防除效果)는 pyrazosulfuron molinate simetryne GR와 dimepiperate bensulfuron dimethametryn GR가 높은 괴불 방제효과(防除效果)를 보였고 담수직파(湛水直播)벼의 약해증상(藥害症狀)은 보이지 않았다.

• 대한핵의학회지
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• 제9권1호
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• pp.51-58
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• 1975

It is a great value to find an early detection of involvement of ureteric obstruction in the carcinoma of cervix. Little or no knowledge of the condition of the kidneys or the lower urinary tract are able to elucidate by the biochemical studies such as blood nitrogen or urine creatinine in carcinoma of cervix. Findings of urography delineates the condition of urinary tract stasis in the renal pelvis and ureters, however, slight stasis maybe difficult to demonstrate. On the other hand isotope nephrography is accepted as a sensitive method to observe renal function especially in regarding to the excretory function of kidney. It was attempted to analysis the findings of urography conjunction with isotope nephrography in 50 cases of unselected patients with invasive carcinoma of cervix through pre and post irradiation follow up studies. Urography was done as a routine procedure and.analysed emphasising changes of collecting systems and ureter condition. Isotope nephrography was carried out by means of $15{\mu}ci\;I^{131}$-Hippuran injected intravenously and the curves were analysed as follows. Parameter were; time of maximum amplitude ($T_{max}$), half time of maximum amplitude ($T\frac{1}{2}$), Kac and Kex value calculated from these two parameters in Tobe's method. The excretion index by Aurell defines the ratio between the maximum activity and the activity measured on the slope of the third phase ten minites after it has reached its maximum. Results: 1. 28.8% had an abnormal IVP suggestive of ureteric involvement before irradiation therapy and the patient of stage III and IV were the great part. 2. 21.7% had abnormal findings of per-irradiation renogram whom showed normal IVP. The other group showed normal IVP which group also showed normal renogram prior irradiation. 3. The more severe the ureteric involvement, the change of excretion index was greater. 4. Even in stage I and II patient, abnormal renogram was revealed in 12 cases (39.4%) among 31 cases. 5. All cases of TAH showed abnormal findings of IVP and renogram. 6. No. definite change of renogram was obtained just after the irradiation therapy (point $A:8000{\sim}9000rads,\;B:5000{\sim}6000rads,\;Co:11000{\sim}13000rads$). Each 3 month follow up study was performed and comparing with preirradiation study which showed significant changes of excretion index of renogram were 42.8% in $6{\sim}9$ month follow-up and 75% in $9{\sim}12$ month, respectively. 7. It seems to be important to observe the parameter Kex and excretion index of renogram to determine early abnormality of kidney excretory function by means of post-irradiation follow up study.

• 한국해양학회지
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• 제28권1호
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• pp.52-68
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• 1993

철과 킬레이트 화합물이 적도용승계에서 일차생산에 미치는 영향이 1989년 TOGA와 EPOCS 항해 기간 중 연구되었다. 식물플랑크톤 현존량의 변화와 철 결핍으로 인한 영 향은 광합성 전자전달계의 저해제인 DCM에 의한 생체형광의 변화를 이용하여 추정하였 다. 질소 신생산은 안정동위원소인 /SUP 15/N KNO$_3$의 흡수를 이용하여 측정하였다. 표층 질산염의 농도가 5 uM 이상인 용승해역에서는 대조군과 킬레이트화된 철 처리군 사이에 생체형광과 질산염 흡수능력에 유의성 있는 차이를 보였다. 그러나 CFC(세포형 광용량)에 있어서는 영양염에 의한 제한이 대조군과 처리군 사이에 유의성 있는 차이 가 나타나지 않았다. 표층 질산염 농도가 낮은 (0.5 uM 이하) 용승해역 바깥은 대조군 과 처리군 사이에 생체형광과 CFC 값에 유의성 있는 차이가 보이지 않았다. 적도 용승 계에서 1차생산과 질소 신생산은 철의 가용도에 의해 제한을 받는 것이 자명하다. 그 러나 CFC 값에 의하면 이 해역에 자생하는 식물플랑크톤의 생리작용은 철 결핍에 의한 영향을 받지않는 것으로 사료된다.