• Journal of Aquaculture
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• v.15 no.1
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• pp.39-42
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• 2002

Water temperature, at which Spisula sachdinensis egg successfully completed development ranges from 10 to $25^{\circ}C$. Within this range, the development duration is reduced with increasing temperature. The relationships between temperature (WT, $^{\circ}C$) and the required time (t: hour) from egg to each developmental stage are described as follows: 2-cell : 1/t = 0.0554 WT -0.3769; 8-cell : 1/t = 0.0210 WT -0.1142; Morula : 1/t = 0.0125WT -0.0712; Trochophore : 1/t = 0.0039 WT -0.0203; D-shaped larva : 1/t = 0.0024W T -0.0108. Biological minimum temperature for the egg development is 5.5$^{\circ}C$.

• Parasites, Hosts and Diseases
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• v.31 no.3
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• pp.249-258
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• 1993

Naegleria fowleri is the cause of primary amoebic meningoencephalitis in man, IL-2 levels after stimulation of T lymphocytes by PHA or N.fowleri lysates. the amounts of T lymphocyte subsets and the blastogenic responses of T lymphocytes in mice after Infected with pathogenic N. fowleri were studied comparing between two study groups, one $1{\;}{\times}{\;}10^4$ trophozoites inoculated mice and the other $1{\;}{\times}{\;}10^5$ trophozoites inoculated mice. All experimental samples were obtained on the day 7, 14 and 24 after inoculation. The mice inoculated with $1{\;}{\times}{\;}10^4$ trophozoites showed a 14.3% mortality rate, and 72.2% in the mice inoculated with $1{\;}{\times}{\;}10^5$ trophozoites. The IL-2 levels on day 14 of two experimental groups were significantly decreased as compared with the control group. Thy 1.2+T cells in the total spleen Iymphocytes of $1{\;}{\times}{\;}10^5$ trophozoites inoculated group on day 7 were significantly increased compared with the control group. There was no significant difference between $1{\;}{\times}{\;}10^4$ trophozoites inoculated group and the control group. $L3T4^{+}{\;}T$ cells and $Ly2^{+}$ T cells in the total spleen Iymphocytes of $1{\;}{\times}{\;}10^5$ trophozoites inoculated group on day 7 were sigrlificantly increased compared with the control group. The DNA S fraction of T cells in the spleen of $1{\;}{\times}{\;}10^5$ trophozoites inoculated group was significantly increased on day 7. The amount of S fractions of DNA were sequentially decreased on day 14 and 24 but they were also signiacantly increased compared with the control group. The results obtained in the experiments indicats that cell mediated immunity after N.fowleri infection acts on very important host's protection immunity around the 7th day after infection. IL-2 level was much suppressed on day 14 which resulted from the exhaustion of host immune response. It was observed that the level of IL-2 production ability and the amounts of T lymphocytes subsets and the blastogenic responses of T lymphocytes were not well correlated during the observation period.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.34 no.2
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• pp.144-158
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• 1998

In order to study basic information on the developed electro-magnetic compass, experiments were carried out on board M. S. KAYA at the pier of Dong Kuk Steel Mill in Pusan and the Korean southern sea using a three-axis magnetic sensor from Jan. 21, 1995 to Feb. 14, 1996. The obtained results were as follows : 1. The amount of old metal on the pier was about 27,290tons~57,440tons with an average of 40,560tons, the artificial local disturbance at the pier was min. 27.1$\mu$T, max. 66.5$\mu$T, ave. 433$\mu$T for the horizontal component and min. -27.0$\mu$T, max. 45.1$\mu$T, ave. 3.7$\mu$T for the vertical component. Its direction of horizontal component was 305$^{\circ}$ with the ship's head up bearing at 225$^{\circ}$. 2. The ship's magnetic distribution on the starboard side on berthing at the pier was 17.4$\mu$T for the horizontal component and -6.2$\mu$T for the vertical component. On the ship's port side, it was 19.8$\mu$T for the horizontal component and 4.1$\mu$T for the vertical component. On the ship's starboard side at sea, the ship's magnetic distribution was 19.2$\mu$T for the horizontal component and 3.2$\mu$T for the vertical component. On the ship's port side, the readings were 22.0$\mu$T for the horizontal component and -1.8$\mu$T for the vertical component. The directions of these readings were nearly starboard side. 3. On the pier, the secular change of the artificial local disturbance decreased 8.3$\mu$T from 61.0$\mu$T to 52.7$\mu$T for the horizontal component and decreased 7.1$\mu$T from 8.9$\mu$T M 1.8$\mu$T for the vertical component. On the starboard side from its berth, the ship, s magnetic distribution increased 2.6$\mu$T from 14.8$\mu$T to 17.4$\mu$T for the horizontal component and increased -0.1$\mu$T from -6.1$\mu$T to -6.2$\mu$T for the vertical component. On the ship's port side from its berth, it increased 7.1$\mu$T from 12.7$\mu$T to 19.8$\mu$T for the horizontal component and increased 10.2$\mu$T from -6.1$\mu$T to 4.1$\mu$T for the vertical component. 4. While at sea, on the ship's starboard side, the Secular change of the ship's magnetic distribution increased 3.9$\mu$T from 15.3$\mu$T to 19.2$\mu$T for the horizontal component and increased 2.0$\mu$T from -5.2$\mu$T to -3.2$\mu$T for the vertical component. On the port side, the changes increased 11.4$\mu$T from 10.6$\mu$T to 22.0$\mu$T for the horizontal component and increased 4.9$\mu$T from -6.7$\mu$T to -1.8$\mu$T for the vertical component. Upon berthing at the pier, the deviation of the secular change increased westerly 1 degree W~ 2.5$^{\circ}$ W from 3.5$^{\circ}$ W~ 5$^{\circ}$ W M 6W with the ship's head up bearing at 225$^{\circ}$. While at sea, these increased westerly 2$^{\circ}$ ~ 3$^{\circ}$ from the Northeast to the South and increased easterly 1$^{\circ}$ ~ 8$^{\circ}$ from the Southwest to the North. 5. While at port, within 1 mile between the ship and berth of the pier, as we approached the pier, the westerly deviation increased and when we departed the pier easterly deviation increased. When approaching the pier, the deviation was smaller than the deviation when the ship was departing from the pier. When approaching the bearing at 225$^{\circ}$ with the ship's head up bearing, the varies of deviation was smaller than the varies when the ship's head up bearing was departing from it.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.6
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• pp.847-854
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• 2014

Different herbs and spices have been used as feed additives for various purposes in poultry production. This study was conducted to assess the effect of feed supplemented with black pepper (Piper nigrum), turmeric powder (Curcuma longa), coriander seeds (Coriandrum sativum) and their combinations on the performance of broilers. A total of 210 (Cobb) one-d-old chicks were divided into seven groups of 30 birds each. The treatments were: a control group received no supplement, 0.5% black pepper (T1), 0.5% turmeric powder (T2), 2% coriander seeds (T3), a mixture of 0.5% black pepper and 0.5% turmeric powder (T4), a mixture of 0.5% black pepper and 2% coriander seed (T5), and a mixture of 0.5% black pepper, 0.5% turmeric powder and 2% coriander seeds (T6). Higher significant values of body weight gain during the whole period of 5 weeks (p<0.001) were observed in broilers on T1, T3, T5, and T6 compared to control. Dietary supplements with T1, T2, T3, and T6 improved the cumulative G:F of broilers during the whole period of 5 weeks (p<0.001) compared with control. The dressing percentage and edible giblets were not influenced by dietary supplements, while higher values of relative weight of the liver (p<0.05) were obtained in T5 and T6 compared to control. The addition of feed supplements in T5 and T6 significantly increased serum total protein and decreased serum glucose, triglycerides and alkaline phosphatase concentrations compared with the control group (p<0.05). Broilers on T6 showed significant decrease in the serum glutamate pyruvate transaminase concentration (p<0.05) compared to control. The broilers having T5 and T6 supplemented feed had relatively greater antibody titre (p<0.001) at 35 d of age than control. It is concluded that dietary supplements with black pepper or coriander seeds or their combinations enhanced the performance and health status of broiler chickens.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.162-168
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• 2022

This study was carried out to investigate the effects of planting densities on the growth characteristics, dry matter yield, and feed value of "Geukdong 6" (a new variety of corn for feed). The experimental design was arranged in a randomized block design with three replications. Treatments consisted of six planting densities, 60 cm × 25 cm (T1), 60 cm × 30 cm (T2), 70 cm × 25 cm (T3), 70 cm × 30 cm (T4), 80 cm × 25 cm (T5) and 80 cm × 30 cm (T6). All treatments were sown on May 14, 2021, and the harvest was on October 3 (late flowering). Plant length and the number of tillers were the highest in T5 (p<0.05), but the number of leaves and stem diameter were the highest in T6 than in the other treatments (p<0.05). Leaf length, leaf width, and dead leaf were not significantly different among the treatments. Organic matter was highest in T6, and crude protein was highest in T5 (p<0.05). The ether extract was not significantly different among the treatments. Crude fiber, NDF, and ADF were highest in T2 with relatively higher planting density (p<0.05). Calcium and phosphorus were not significantly different among the treatments. TDN content was the highest in T3 (p<0.05). Sugar degree (Brix), fructose, glucose, dextran, isomerose, and inverted sugar were not significantly different among the treatment. Fresh yield, dry matter yield and TDN yield were higher in order of T6 > T5 > T4 > T3 > T2 > T1 (p<0.05). Relatively feed value was higher in order of T3 > T6 > T5 > T1 > T4 > T2 (p<0.05). Based on the above results, planting density could be recommended from 80 cm × 30 cm for efficient production of "Geukdong 6".

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.9
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• pp.1449-1457
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• 2018

Objective: The objective was to evaluate feed intake, digestibility, body weight change and carcass characteristics of sheep fed a basal diet of hay supplemented with banana leaves and silver leaf desmodium. Methods: Thirty yearling lambs with an average initial body weight of $15.85{\pm}1.6kg$ were grouped into six blocks of five rams in each block. The treatments were: hay alone (T1), hay+100% banana leaf (T2), hay+67% banana leaf+33% desmodium leaf (T3), hay+33% banana leaf+67% desmodium leaf (T4), andhay+100% desmodium leaf (T5). Three hundred grams of treatment diets were offered daily on as fed basis. The feeding and digestibility trials lasted for 84 and 7 days, respectively, followed by carcass evaluation. Results: The total dry matter (DM) intake for T3, T4, and T5 were greater (p<0.05) than those fed T1 and T2 diets. The lowest (p<0.05) organic matter (OM) intake was recorded in rams reared on T1 diet. The total crude protein (CP) intake was in the following order: T5>T4>T3>T2>T1. Ram lambs receiving supplementary diets had higher (p<0.05) DM, OM, CP, neutral detergent fiber and acid detergent fiber digestibility compared with the control diet. The empty body weight and slaughter weight was highest (p<0.05) in rams receiving T3, T4, and T5 diets. The average daily gain and feed conversion efficiency was highest (p<0.05) in rams receiving the supplementary diets. The dressing percentage on the basis of hot carcass weight linearly increased with increasing levels of desmodium. Rams reared on supplementary diet had higher (p<0.05) rib eye area compared with the control diet. Conclusion: In conclusion, when banana leaf is used as a supplement to poor quality grass, better body weight gain was obtained when fed in combination with desmodium.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.86-92
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• 2008

This study was conducted to determine removal effect on phytoplankton (chlorophyll-${\alpha}$ as whole algae) and cyanobacteria by a fish Pseudorasbora parva and macroinvertebrate Palaemon paucidens in September 2006. Three treatments with 25 (T1), 50 (T2) and 100 (T3) individuals along with control (C1, no input fish), and two treatments with 25 (T4) and 50 (T5) individuals along with control (C1) were made for fish and macroinvertebrate, respectively. The initial concentrations of chlorophyll-${\alpha}$ $(Chl_i)$ in each 10L test tank were set up for the levels of $95{\sim}100{\mu}g\;L^{-1}$ and the daily values were monitored in the test tank during 7 days. In the lab tests, P. parva did not show Chl-${alpha}$ removal effect; the removal rate of Chl-${alpha}$ for P. parva was -58% in T1, -56% in T2, and 61% in T3 during the test period. In contrast, P. paucidens. in the treatments of T4 and T5 removed the phytoplankton effectively and the removal effect were appeared to be 33% and 22%, respectively. Also, P. paucidens showed high feeding efficiency in the removal of cyanobacteria. The levels of cyanobacteria were greatly lowed from 6,048 to 927 cells $mL^{-1}$ in T4 and from 6,539 to 1,053 cells $mL^{-1}$ in T5, resulting in 85% and 84% in the removal effect, respectively. Our results for biomanipulation tests suggest that P. paucidens may be used as a potential candidate organism for algae control in spite of the preliminary results by laboratory tests.

• Food Science of Animal Resources
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• v.37 no.1
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• pp.10-17
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• 2017

This effect of Monascus and Laccaic acid on the chemical composition, physical, texture and sensory properties of sausage were investigated during storage. Eight treatments (T) of sausage such as T1 (12 ppm sodium nitrite), while T2, T3, T4, T5, T6 and T7 were formulated with different ratios of Monascus/Laccaic acid: 63/7.0, 108/12, 135/15, 59.5/10.5, 102/18 and 127.5/22.5 ppm, respectively. The batch formulated without nitrite or Monascus and laccaic acid was served as control (C). The control sausages had higher pH values compared to the treated ones at 3, 10 and 28 d storage (p<0.05). After 10 d storage, the pH values decreased in treated sausage samples (p<0.05). The T1 and T4 presented the lowest yellowness and lightness values, respectively over the storage period. The redness values were increased as increasing Monascus and Laccaic acid amounts (T2-T4, T5-T7). The addition of Monascus and Laccaic acid had significantly higher hardness and springiness values (p<0.05) compared with the control in 3, 19 or 28 d storage. The results indicated that the addition of Monascus and Laccaic acid could improve the redness of the products.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.43-52
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• 2004

This study was carried out to determine the effect of corn silage and roughage feeding systems on milk yield and milk compositions. The experimental design was allotted into 3 treatments according to the feeding systems of rice straw(rice straw＋concentrate: T1), corn silage(corn silage ＋ concentrate: T2) and TMR treatment(Total mixed rate : roughage ＋ concentrate: T3). This research was carried out from Oct. 1988 to Mar. 1999 at Kimcheon Kyungbook. The results obtained were summarized as follows: 1. The milk yield increased upon T2(31.9$\pm$3.2kg) ＞ T3(29.6$\pm$3.8kg) ＞ T1 treatment(22.5$\pm$2.0kg)(P<0.05), but cows with T2 and T3 produced the highest at third parity while T1 at forth parity. 2. The milk fat percentage was the highest at T3 treatment(3.79$\pm$0.31%), and on the other hand T1 treatment appeared the lowest percentage. Cow at 2nd parity produced the highest milk fat contend over the other parity. 3. There was no significant difference in protein content between treatments and parity, but T2 and T3 were higher than T1 4. Treatment did not affact content of SNF even though T2 and T3 of the SNF revealed to higher than T1. T5 was T3(12.51$\pm$0.57%)>T1(11.71$\pm$0.62%)>T2 treatment(11.52$\pm$0.55%). These were not significant. 5. Somatic cell counts were the highest at T1(39.6 ${\times}$ $10^4$cell/ml), but T2 treatment was the lowest as 28.7 ${\times}$ $10^4$ cell/ml. These results indicates that com silage(T2) and TMR treatment(T3) could be recommended

• Journal of Microbiology and Biotechnology
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• v.33 no.7
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• pp.909-914
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• 2023

While searching for the bacteria which are responsible for degradation of pesticide in soybean field soil, a novel bacterial strain, designated 5-5^T, was isolated. The cells of the strain were Gram-staining-positive, aerobic and non-motile rods. Growth occurred at 10-42℃ (optimum, 30℃), pH 5.5-9.0 (optimum, pH 7.0-7.5), and 0-2% (w/v) NaCl (optimum, 1%). The predominant fatty acids were C_15:0 anteiso, C_17:0 anteiso, and summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c). The predominant menaquinone was MK-9 (H₂). Diphosphatidylglycerol, glycolipids, phosphatidylinositol, and phosphatidylglycerol were the major polar lipids. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 5-5^T is a member of the genus Sinomonas and its closest relative is Sinomonas humi MUSC 117^T, sharing a genetic similarity of 98.4%. The draft genome of strain 5-5^T was 4,727,205 bp long with an N50 contig of 4,464,284 bp. Genomic DNA G+C content of strain 5-5^T was68.0 mol%. The average nucleotide identity (ANI) values between strain 5-5^T and its closest strains S. humi MUSC 117^T and S. susongensis A31^T were 87.0, and 84.3 % respectively. In silico DNA-DNA hybridization values between strain 5-5^T and its closest strains S. humi MUSC 117^T and S. susongensis A31^T were 32.5% and 27.9% respectively. Based on the ANI and in silico DNA-DNA hybridization analyses, the 5-5^T strain was considered as novel species belonging to the genus Sinomonas. On the basis of the results from phenotypic, genotypic and chemotaxonomic analyses, strain 5-5^T represents a novel speciesof the genus Sinomonas, for which the name Sinomonas terrae sp. nov. is proposed. The type strain is 5-5^T (=KCTC 49650^T =NBRC 115790^T).