• 제목/요약/키워드: 1-sequence covering

검색결과 28건 처리시간 0.028초

Chromosomal Information of 1,144 Korean BAC Clones

  • Park, Mi-Hyun;Lee, Hee-Jung;Kim, Kwang-Joong;Jeon, Jae-Pil;Lee, Hye-Ja;Kim, Jun-Woo;Kim, Hung-Tae;Cha, Hyo-Soung;Kim, Cheol-Hwan;Choi, Kang-Yell;Park, Chan;Oh, Berm-Seok;Kim, Ku-Chan
    • Genomics & Informatics
    • /
    • 제4권4호
    • /
    • pp.141-146
    • /
    • 2006
  • We sequenced 1,841 BAC clones by terminal sequencing, and 1,830 of these clones were characterized with regard to their human chromosomal location and gene content using Korean BAC library constructed at the Korean Science (KCGS). Sequence analyses of the 1,830 BAC clones was performed for chromosomal assignment: 1,144 clones were assigned to a single chromosome, 190 clones apparently assigned to more than one chromosome, and 496 clones to no chromosome. Evaluating gene content of the 1,144 BAC clones, we found that 706 clones represented 1,069 genes of which 415 genes existed in the BAC clones covering the full sequence of the gene, 180 genes covering a $50%{\sim}99%$, and 474 genes covering less than 50% of the gene coverage. The estimated covering size of the KBAC clones was 73,379 kilobases (kb), in total corresponding to 2.3% of haploid human genome sequence. The identified BAC clones will be a public genomic resource for mapped clones for diagnostic and functional studies by Korean scientists and investigators worldwide.

ON COVERING AND QUOTIENT MAPS FOR 𝓘𝒦-CONVERGENCE IN TOPOLOGICAL SPACES

  • Debajit Hazarika;Ankur Sharmah
    • 대한수학회논문집
    • /
    • 제38권1호
    • /
    • pp.267-280
    • /
    • 2023
  • In this article, we show that the family of all 𝓘𝒦-open subsets in a topological space forms a topology if 𝒦 is a maximal ideal. We introduce the notion of 𝓘𝒦-covering map and investigate some basic properties. The notion of quotient map is studied in the context of 𝓘𝒦-convergence and the relationship between 𝓘𝒦-continuity and 𝓘𝒦-quotient map is established. We show that for a maximal ideal 𝒦, the properties of continuity and preserving 𝓘𝒦-convergence of a function defined on X coincide if and only if X is an 𝓘𝒦-sequential space.

Lifting 기법을 이용한 Generalized Bilinear Cover Inequality (Generalized Bilinear Cover Inequality via Lifting)

  • 정광헌
    • 한국경영과학회지
    • /
    • 제42권3호
    • /
    • pp.1-12
    • /
    • 2017
  • In this paper, we generalize lifted inequalities to a 0-1 mixed-integer bilinear covering set with linear terms. This work is motivated by the observation that Generalized Bilinear Inequality (GBI) occurs in the Branch and Bound process. We find some conditions and prove the subadditivity of lifting functions for lifting to be sequence-independent. Using the theoretical results, we develop facet-defining inequalities for a GBI-defined set through three steps of lifting.

Lecanora neobarkmaniana (Lecanorales, Lecanoraceae), A New Lichen Species from South Korea

  • Jung Shin Park;Sang-Kuk Han;Soon-Ok Oh
    • Mycobiology
    • /
    • 제51권1호
    • /
    • pp.16-25
    • /
    • 2023
  • Lecanora is one of the largest genera of lichens worldwide. These lichens can be easily seen, and are commonly found on trees and rocks. Most Korean Lecanora species belong to the Lecanora subfusca group, which has well-defined superficial thallus, red-brown apothecia, and soredia. The new species of L. neobarkmaniana grows on rocks, farinose soredia coalescing, usually covering the whole thallus, and containing atranorin and zeorin. We used internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequence data to identify the phylogenetic relationship across Lecanora sequence data and found the species to form different clades. In this study, we reported some interesting findings and described the genetic relationship with other sorediate Lecanora species and the characteristics of the new species. An identification key for the Korean sorediate Lecanora species is given.

Infectious bursal disease virus(국내분리주)의 variable VP2 gene의 분석 (Sequence analysis of the variable VP2 gene of infectious bursal disease viruses isolated in Korea)

  • 권혁무;김대규;성환우
    • 대한수의학회지
    • /
    • 제39권3호
    • /
    • pp.545-553
    • /
    • 1999
  • A 474-base pair segment covering the hypervariable region of VP2 gene from six Korean infectious bursal disease virus(K-IBDV) isolates(K1, K2, SH/92, 225, 269, 310) and one attenuated IBDV(DAE) were amplified using RT-PCR, sequenced, and compared with published sequences for IBDV. K-IBDV isolates(K1, K2, SH/92, 225, 269) and foreign very virulent(vv) IBDV strains had 94.93~100% amino acid sequence similarity. K-IBDV isolate 310 and other K-IBDV isolates had 84.31~86.07% amino acid sequence similarity. Attenuated strain(DAE), like other attenuated strain, has substitution at positions 279(D to N) and 284(A to T) as well as in the serine-rich heptapeptide region. Five K-IBDV isolates except 310 isolate share unique amino acid residues at positions 222(A), 256(I), 294(I) which are not present in other standard and attenuated strains. At the two hydrophilic region, K-IBDV isolates except 310 isolate had identical amino acids comparing with Belgium vv IBDV 894VB but had four amino acid substitutions comparing with Chinese vv IBDV F9502. The SWSASGS heptapeptide is conserved in all K-IBDV isolates. The sequence of K-IBDV isolate 310 was markedly different from other IBDV strains, evolving from a separate lineage than the others. By phylogenetic analysis, Five K-IBDV isolates except 310 isolate were categorized in one group with foreign vv IBDV isolates but K-IBDV isolate 310 was categorized in a separate group which was differentiated from other compared IBDV strains.

  • PDF

Evaluation of DNA Microarray Approach for Identifying Strain-Specific Genes

  • Hwang, Keum-Ok;Cho, Jae-Chang
    • Journal of Microbiology and Biotechnology
    • /
    • 제16권11호
    • /
    • pp.1773-1777
    • /
    • 2006
  • We evaluated the usefulness of DNA microarray as a comparative genomics tool, and tested the validity of the cutoff values for defining absent genes in test genomes. Three genome-sequenced E. coli strains (K-12, EDL933, and CFT073) were subjected to comparative genomic hybridization with DNA microarrays covering almost all ORFs of the reference strain K-12, and the microarray results were compared with the results obtained from in silico analyses of genome sequences. For defining the K-12 ORFs absent in test genomes (reference strain-specific ORFs), we applied and evaluated the cutoff level of -1. The average sequence similarity between ORFs, to which corresponding spots showed a log-ratio of>-1, was $96.9{\pm}4.8$. The numbers of spots showing a log-ratio of <-1 (P<0.05, t-test) were 90 (2.5%) and 417 (10.6%) for the EDL933 genome and the CFT073 genome, respectively. Frequency of false negatives (FN) was ca. 0.2, and the cutoff level of -1.3 was required to achieve the FN of 0.1. The average sequence similarity of the false negative ORFs was $77.8{\pm}14.8$, indicating that the majority of the false negatives were caused by highly divergent genes. We concluded that the microarray is useful for identifying missing or divergent ORFs in closely related prokaryotic genomes.

Phylogenetic analysiccccccccc of the genus Stemphylium based on elongation factor -1 alpha and calmodulin gene squences

  • Kong, D.W.;Cho, H.S.;Yu, S.H.
    • 한국식물병리학회:학술대회논문집
    • /
    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
    • /
    • pp.117.2-117
    • /
    • 2003
  • The importance and diversity of the genus Stemphylium highlights the need for accurate identification of species. However, many Stemphylium isolates have been misidentified due to the use of spore size as the only identifying character. Molecular phylogenetic analyses were performed on fifty-four isolates covering 9 Stemphylium species collected in Korea. Phylogenetic analysis of the translation elongation factor -1 alpha (EF-1) and the calmodulin gene sequence data showed that Stemphylium species were segregated into seven distinct groups, most of w hichcorrelated with species identified by morphology. Analysis of EF-1 in particular was useful for establishing well- supported relationships among the species of Stemphylium.

  • PDF

The Role of Dynamic Contrast Enhanced MR Mammography in Differentiation between Benign and Malignant Breast Lesions

  • 한송이;차은숙;정상설;김학희;변재영;이재문
    • 대한자기공명의과학회:학술대회논문집
    • /
    • 대한자기공명의과학회 2002년도 제7차 학술대회 초록집
    • /
    • pp.135-135
    • /
    • 2002
  • Purpose: To assess diagnostic accuracy of dynamic contrast enhanced MR mammography in differentiating between benign and malignant lesions. Materials and methods: Ninety-three patients with suspicious mammographic, sonographic or palpable findings underwent pre- or postoperative contrast-enhanced MR imaging of breast using three dimensional fast low-angle shot (3D FLASH) sequence (16/4 msec[repetition time / echo time], 20 flip angle, 3mm slice thickness with no slice gap, 256 by 256 in-plane matrix) covering whole breasts. T1 weighted images were obtained before and after bolus administration of gadopentetate dimeglumine (0.15 mmol/kg). Subtraction images and time-signal intensity curves of region of interest were obtained sequentially and correlated with pathologic diagnoses of lesions.

  • PDF

Zero-one Integer Programming Approach to Determine the Minimum Break Point Set in Multi-loop and Parallel Networks

  • Moirangthem, Joymala;Dash, Subhransu Sekhar;Ramaswami, Ramas
    • Journal of Electrical Engineering and Technology
    • /
    • 제7권2호
    • /
    • pp.151-156
    • /
    • 2012
  • The current study presents a zero-one integer programming approach to determine the minimum break point set for the coordination of directional relays. First, the network is reduced if there are any parallel lines or three-end nodes. Second, all the directed loops are enumerated to reduce the iteration. Finally, the problem is formulated as a set-covering problem, and the break point set is determined using the zero-one integer programming technique. Arbitrary starting relay locations and the arbitrary consideration of relay sequence to set and coordinate relays result in navigating the loops many times and futile attempts to achieve system-wide relay coordination. These algorithms are compared with the existing methods, and the results are presented. The problem is formulated as a setcovering problem solved by the zero-one integer programming approach using LINGO 12, an optimization modeling software.