Browse > Article

Chromosomal Information of 1,144 Korean BAC Clones  

Park, Mi-Hyun (Center for Genome Science, National Institute of Health)
Lee, Hee-Jung (Center for Genome Science, National Institute of Health)
Kim, Kwang-Joong (Center for Genome Science, National Institute of Health)
Jeon, Jae-Pil (Center for Genome Science, National Institute of Health)
Lee, Hye-Ja (Center for Genome Science, National Institute of Health)
Kim, Jun-Woo (Center for Genome Science, National Institute of Health)
Kim, Hung-Tae (Center for Genome Science, National Institute of Health)
Cha, Hyo-Soung (Center for Genome Science, National Institute of Health)
Kim, Cheol-Hwan (Dong-A Seelfch, Co.Ltd,)
Choi, Kang-Yell (Department of Biotechnology, Yonsei University)
Park, Chan (Center for Genome Science, National Institute of Health)
Oh, Berm-Seok (Center for Genome Science, National Institute of Health)
Kim, Ku-Chan (Center for Genome Science, National Institute of Health)
Publication Information
Genomics & Informatics / v.4, no.4, 2006 , pp. 141-146 More about this Journal
Abstract
We sequenced 1,841 BAC clones by terminal sequencing, and 1,830 of these clones were characterized with regard to their human chromosomal location and gene content using Korean BAC library constructed at the Korean Science (KCGS). Sequence analyses of the 1,830 BAC clones was performed for chromosomal assignment: 1,144 clones were assigned to a single chromosome, 190 clones apparently assigned to more than one chromosome, and 496 clones to no chromosome. Evaluating gene content of the 1,144 BAC clones, we found that 706 clones represented 1,069 genes of which 415 genes existed in the BAC clones covering the full sequence of the gene, 180 genes covering a $50%{\sim}99%$, and 474 genes covering less than 50% of the gene coverage. The estimated covering size of the KBAC clones was 73,379 kilobases (kb), in total corresponding to 2.3% of haploid human genome sequence. The identified BAC clones will be a public genomic resource for mapped clones for diagnostic and functional studies by Korean scientists and investigators worldwide.
Keywords
Korean; BAC clone; genome; cancer;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Albertson, D.G. and Pinkel, D. (2003). Genomic microarrays in human genetic diseases and cancer. Hum Mol. Genet. 12, 145-152   DOI   ScienceOn
2 Deloukas, P., Matthews, L.H., Ashurst, J., Burton, J., Gilbert, J.G., Jones, M., Stavrides, G., Almeida, J.P., Babbage, A.K., Bagguley, C.L. et al. (2001). The DNA sequence and comparative analysis of human chromosome 20. Nature 414, 865-871   DOI   ScienceOn
3 Dunham, I., Shimizu, N., Roe, B.A., Chissoe, S., Hunt, A.R, Collins, J.E., Bruskiewich, R., Beare, D.M., Clamp, M., Smink, L.J. et al. (1999). The DNA sequence of human chromosome 22, Nature 402, 489-495   DOI   ScienceOn
4 Hattori, M. (2000). Human genome sequencing. Tanpakushitsu Kakusan Koso 45, 1978-1985
5 Kim, S.Y., Horrigan, S.K., Altenhofen, J.L., Arbieva, Z.H., Hoffman, R., Westbrook, C.A.(1998). Modification of bacterial artificial chromosome clones using Cre recombinase: introduction of selectable markers for expression in eukaryotic cells. Genome Res 8, 404-412   DOI
6 Roest, Crollius, H., Jaillon, O., Bernot, A., Dasilva, C., Bouneau, L., Fischer, C., Fizames, C., Wincker, P., Brottier, P., Quetier, F., Saurin, W., and Weissenbach, J. (2000). Estimate of human gene number provided by genome-wide analysis using Tetraodon nigroviridis DNA sequence. Nat. Genet. 25, 235-238   DOI   ScienceOn
7 Bochukova, E.G., Jefferson, A., Francis, M.J., and Monaco, A.P. (2003). Genomic studies of gene expression: regulation of the Wilson disease gene. Genomics 81, 531-542   DOI   ScienceOn
8 Veltman, J.A., Schoenmakers, E.F., Eussen, B.H., Janssen, I., Merkx, G., van Cleef, B., van Ravenswaaij, C.M., Brunner, H.G., Smeets, D., and Van Kessel. A.G. (2002). High-throughput analysis of subtelomeric chromosome rearrangements by use of array-based comparative genomic hybridization. Am. J. Hum. Genet. 70, 1269-1276   DOI   ScienceOn
9 Lander, E.S., Linton, L.M., Birren, B., Nusbaum, C., Zody, M.C., Baldwin, J., Devon, K., Dewar, K., Doyle, M., FitzHugh, W. et al. (2001). Initial sequencing and analysis of the human genome. Nature 409, 860-921   DOI   ScienceOn
10 Hillier, L.W., Fulton, R.S., Fulton, L.A., Graves, T.A., Pepin, KH, Wagner-McPherson C, Layman, D., Maas, J., Jaeger, S., Walker, R. et al. (2003). The DNA sequence of human chromosome 7. Nature 424, 157-164   DOI   ScienceOn
11 McPherson, J.D., Marra, M., Hillier, L., Waterston, R.H., Chinwalla, A., Wallis, J., Sekhon, M., Wylie, K., Mardis, E.R., Wilson, R.K. et al.(2001). A physical map of the human genome. Nature 409, 934-941   DOI   ScienceOn
12 Hart, S.L., Arancibia-Carcamo, C.V., Wolfert, M.A., Mailhos, C., O'Reilly, N.J., Ali, R.R., Coutelle, C., George, A.J., Harbottle, R.P., Knight, A.M. et al. (1998). Lipid-mediated enhancement of transfection by a nonviral integrin-targeting vector. Hum. Gene Ther. 9, 575-585   DOI   ScienceOn
13 Heilig, R., Eckenberg, R., Petit, J.L., Fonknechten, N., Da Silva, C., Cattolico, L., Levy, M., Barbe, V., de Berardinis, V., Ureta-Vidal, A. et al. (2003). The DNA sequence and analysis of human chromosome 14. Nature 421, 601-601   DOI   ScienceOn
14 Mantripragada, K.K., Buckley, P.G., de Stahl, T.D., Dumanski, J.P. (2004). Genomic microarrays in the spotlight. Trends Genet 20, 87-94   DOI   ScienceOn
15 Wang, Z., Engle,r P., Longacre, A., and Storb, U. (2001). An efficient method for high-fidelity BAC/PAC retrofitting with a selectable marker for mammalian cell transfection. Genome Res. 11, 137-142   DOI
16 International Human Genome Sequencing Consortium. (2004). Finishing the euchromatic sequence of the human genome. Nature 431, 931-945   DOI   ScienceOn
17 Mejia, J.E., and Monaco, A.P. (1997). "Retrofitting vectors for Escherichia coli-based artificial chromosomes (PACs and BACs) with markers for transfection studies", Genome Res. 7, 179-186   DOI   ScienceOn
18 Kim, U.J., Birren, B.W., Slepak, T., Mancino, V., Boysen, C., Kang, H.L., Simon, M.I., Shizuya, H.(1996). Construction and characterization of a human bacterial artificial chromosome library. Genomics 34, 213-218   DOI   ScienceOn
19 Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). "Molecular Cloning: A Laboratory Manual", 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
20 Wade-Martins, R., Smith, E.R., Tyminski, E., Chiocca, E.A., and Saeki, Y. (2001). An infectious transfer and expression system for genomic DNA loci in human and mouse cells. Nat Biotechnol. 19, 1067-1070   DOI   ScienceOn
21 Park, M.H., Lee, H.J., Bok, J., Kim, C.H., Hong, S.T., Park, C., Kimm, K., Oh B., and Lee, J.Y. (2006). Korean BAC library construction and characterization of HLA-DRA, HLA-DRB3. J Biochem Mol Biol. 39(4):418-425   DOI
22 Shizuya, H., Birren, B., Kim, U.J., Mancino, V., Slepak, T., Tachiiri, Y, and Simon, M. (1992). Cloning and stable maintenance of 300-kilobasepair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794-8797