• Journal of Embryo Transfer
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• v.15 no.1
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• pp.103-108
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• 2000

The object of this study was to find simple and effective methods for the speculation of vitality and scorsome status of bovine spermatozoa. The eosin-nigrosin staining, trypan blue staining, and naphthol yellow S-erythrosin B staining was ofter used for the speculation of vitality and/or acrosome status of bovine spermatozoa, respectively. This study has shown that the combined trypan blue-naphthol yellow S-erythrosin B staining is more accurate and effective for the examination of acrosome status and vitality of bovine spermatozoa.

• Journal of Preventive Medicine and Public Health
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• v.41 no.1
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• pp.61-67
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• 2008

Objectives : Polycyclic aromatic hydrocarbons (PAH) and toluene have been reported to induce reactive oxygen species and oxidative stress. This study was performed to investigate the effects of low level exposure to PAHs or toluene on the lipid peroxidation level in elementary school children and the elderly in a rural area. Methods : Forty seven elementary school children and 40 elderly people who were living in a rural area and not occupationally exposed to PAH or toluene were the subjects of this study. Information about active or passive smoking and diet was obtained using a self-administered questionnaire. The urinary 1-hydroxypyrene (1-OHP), 2-naphthol, hippuric acid and thiobarbituric acid reactive substance (TBARS) concentrations were measured, and these values were corrected with the urinary creatinine concentration. Results : In school children, the geometric means of the urinary 1-OHP, 2-naphthol, hippuric acid and TBARS levels were $0.02\;{\mu}mol/mol$ creatinine, $0.47\;{\mu}mol/mol$ creatinine, 0.14 g/g creatinine and $0.95\;{\mu}mol/g$ creatinine, respectively. Those values for the elderly were $0.07{\mu}mol/mol$ creatinine, $1.87{\mu}mol/mol$ creatinine, 0.11 g/g creatinine and $1.18\;{\mu}mol/g$ creatinine, respectively. The mean levels of urinary 1-OHP, 2-naphthol and TBARS were significantly higher in the elderly subjects than in the children. The urinary TBARS level was not correlated with the urinary 1-OHP, 2-naphthol and hippuric acid, but they were correlated with the age of the subjects. Conclusions : These results suggest that low level inhalation exposure to PAH or toluene does not markedly increase lipid peroxidation, and age is a significant determinant of lipid peroxidation.

• Journal of the Korean Applied Science and Technology
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• v.7 no.1
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• pp.107-114
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• 1990

Optical resolution of aminoalcohols had been achived efficiently by use of (R)-2,2'-dihydroxy-l,1'-binaphtholphosphoric acid as the resolving agent in various organic solvents. Racemic aminoalcohols were resolved very easily and high enantiomer yield (about 70% e,e) in THF. On the other hand, absolute configuration of resolved aminoalcohol was (R)-configuration but one of the Valinnol and Phhenylglycinol was anti-type because of sterichindrance.Optical resolution of aminoalcohols had been achived efficiently by use of (R)-2,2'-dihydroxy-1,1'-binaphtholphosphoric acid as the resolving agent in various organic solvents. Racemic aminoalcohols were resolved very easily and high enantiomer yield (about 70% e,e) in THF. On the other hand, absolute configuration of resolved aminoalcohol was (R)-configuration but one of the Valinnol and Phhenylglycinol was anti-type because of sterichindrance.

• Bulletin of the Korean Chemical Society
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• v.24 no.3
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• pp.291-294
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• 2003

A highly interferent-resistive membrane, poly-5-amino-1-naphthol (poly-5A1N), underlied beneath enzymeembedded poly-1,3-phenylendiamine (poly-m-PD) network for miniturized continuous monitoring glucose sensors. The enzyme layer was prepared from a mixed solution of glucose oxidase (GOx) and m-PD monomer by simple electrolysis. The mass change of poly-5A1N was monitored by electrochemical quartz crystal microbalance (EQCM) in situ and the corresponding thickness was measured. Successive electropolymerization of poly-5A1N and poly-m-PD create a several tens nm-thick bilayer showing excellent selectivity for $H_2O_2$ and low activity loss of immobilized enzymes.

• Bulletin of the Korean Chemical Society
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• v.32 no.1
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• pp.153-156
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• 2011

A concise synthesis of (${\pm}$)-rhinacanthin A is achieved in two steps by epoxidation of dehydro-$\alpha$-lapachone, followed by chemo- and regioselective reduction. Dehydro-$\alpha$-lapachone was also synthesized in two steps starting from 4-methoxy-1-naphthol by ethylenediamine diaetate (EDDA)-catalyzed benzopyran formation and a CAN-mediated oxidation reaction. $\beta$-Lapachone was synthesized in three steps from 4-methoxy-1-naphthol by benzopyran formation, catalytic hydrogenation, and Jones oxidation. As additional reactions, synthesis of pyranonaphthoquinone derivatives with the pyranokunthone B skeleton has been achieved in a single step from readily available 2-hydroxy-6-methoxy-1,4-naphthoquinone and 2-hydroxy-7-methoxy-1,4-naphthoquinone.

• Bulletin of the Korean Chemical Society
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• v.29 no.10
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• pp.1932-1936
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• 2008

A new analytical method using 1-(2-pyridylazo)-2-naphthol modified $SiO_2$ nanoparticles as solid-phase extractant has been developed for the preconcentration of trace amounts of mercury(II) in different water samples. Conditions of the analysis such as preconcentration time, effect of pH, sample volumes, shaking time, elution conditions and effects of interfering ions for the recovery of analyte were investigated. The adsorption capacity of nanometer $SiO_2$-PAN was found to be 260 ${\mu}molg^{-1}$ at optimum pH and the detection limit (3$\sigma$) was 0.48 ${\mu}gL^{-1}$. The extractant showed rapid kinetic sorption. The adsorption equilibrium of mercury(II) on nanometer $SiO_2$-PAN was achieved just in 5 mins. Adsorbed mercury(II) was easily eluted with 5 mL of 6 M hydrochloric acid. The maximum preconcentration factor was 50. The method was applied for the determination of trace amounts of mercury(II) in various water samples and industrial effluents.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2005.05a
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• pp.276-277
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• 2005

PHAs와 같은 유해유기화학물질의 환경노출에 따른 건강영향을 파악하기 위해서 요중대사산물의 배설량, 유전적감수성 등의 생체지표분석법을 동시에 측정하여 정확한 노출량을 평가하고자 하였다. 또한 직업적, 비직업적 노출인구에 영향을 미칠 수 있는 흡연, 음주, 식이 등의 생활습관과의 상호관련성도 평가하였다. 그 결과 비교적 소량의 PAHs에 노출되는 일반인구에서 요중 1-OHP 및 2-naphthol의 배설량에 가장 큰 영향을 미치는 인자는 흡연이었으며 요중 1-OHP의 배설량은 음주여부, GSTM1 유전자의 다형성에 의해서도 영향을 받음을 알 수 있었다.

• Journal of the Korean Chemical Society
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• v.29 no.3
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• pp.197-204
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• 1985

The dependence of polarographic parameters on pressure and temperature for the reduction of 2.0 ${\times}\;10^{-4}$M 1-(2-pyridylazo)-2-naphthol (PAN) and 5.0 ${\times}\;10^{-4}$M 4-(2-pyridylazo)-resorcinol(PAR) in buffered methanol-water mixed solution at the dropping mercury electrode, has been discussed. In this experiment the temperature is varied from 25$^{\circ}C$ to 35$^{\circ}C$ and the pressure is ranging from 1 atmosphere to 1,800 atmospheres. The reduction half-wave potentials are shifted to the more positive potentials with increase in pressure. The diffusion currents of two depolarizers become considerably larger with increase in pressure from 1 atmosphere to about 1,000 atmospheres but are getting smaller above 1,000 atmospheres. The slopes of log plot become much larger with increase in pressure, which indicates the more irreversible reduction. The reduction currents of two depolarizers are found to be diffusion controlled over all pressure ranges. The linear relationships between diffusion current and the concentration of two depolarizers are good agreement over all pressure ranges (1 atm∼1,800atm.).

• Journal of Environmental Health Sciences
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• v.37 no.6
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• pp.474-481
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• 2011

This study investigated urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) in the urine of smokers and non-smokers by liquid chromatography triple quordrupole tandem mass spectroscopy (LC/MS/MS). Compounds analyzed for urinary biomarkers of PAHs were five mono-hydroxylated PAHs metabolites; 1-naphthol, 2-naphthol, 1-hydroxypyrene(1-OHP), 3-phenanthrol, 2-fluorenol. Urine samples were pretreated by enzymatic hydrolysis and solid phase extraction method. Smokers were composed of 17 men and five women; non-smokers 17 men and 16 women. Smoking increased urinary concentrations of five PAHs metabolites significantly higher than those of nonsmokers. Statistically significant correlations among the five PAHs metabolites were shown. The results suggest that LC/MS/MS technology should be useful in the environmental health discipline.

• Journal of the Korean Chemical Society
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• v.37 no.8
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• pp.723-729
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• 1993

Cu(II) ion-responsive chemically modifed electrodes (CMEs) were constructed by incorporating 1-(2-pyridylazo)-2-naphthol (PAN) into a conventional carbon-paste mixture of graphite powder and Nujol oil. Cu(II) ion was chemically deposited on the surface of the PAN-chemically modified electrode in the absence of an applied potential by immersion of the electrode in a buffer solution (pH 3.2) containing Cu(II) ion, and then reduced at a constant potential in 0.1 M KNO$_3$. And a well-defined voltammetric peak could be obtained by scanning the potential to the positive direction. The electrode surface could be regenerated with exposure to acid solution and reused for the determination of Cu(II) ion. In 5 deposition / measurement / regeneration cycles, the response could be reproduced with 6.1${\%}$ relative standard deviation. In case of using the differential pulse voltammetry, the calibration curve for Cu(II) was linear over the range of 2.0 ${times}$ 10$^{-7}$ ∼ 1.0 ${times}$ 10$^{-6}$ M. And the detection limit was 6.0 ${times}$ 10$^{-8}$ M. Studies of the effect of diverse ions showed that Co, Ni, Zn, Pb, Mg and Ag ions added 10 times more than Cu(II) ion did not influence on the determination of Cu(II) ion, except EDTA and oxalate ions.