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http://dx.doi.org/10.5668/JEHS.2011.37.6.474

Comparison of Concentration of Urinary Metabolites of PAHs from Smokers and Nonsmokers  

Kho, Young-Lim (Department of Health, Environment & Safety, Eulji University)
Lee, Eun-Hee (Graduate School of Health Science, Far East University)
Publication Information
Journal of Environmental Health Sciences / v.37, no.6, 2011 , pp. 474-481 More about this Journal
Abstract
This study investigated urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) in the urine of smokers and non-smokers by liquid chromatography triple quordrupole tandem mass spectroscopy (LC/MS/MS). Compounds analyzed for urinary biomarkers of PAHs were five mono-hydroxylated PAHs metabolites; 1-naphthol, 2-naphthol, 1-hydroxypyrene(1-OHP), 3-phenanthrol, 2-fluorenol. Urine samples were pretreated by enzymatic hydrolysis and solid phase extraction method. Smokers were composed of 17 men and five women; non-smokers 17 men and 16 women. Smoking increased urinary concentrations of five PAHs metabolites significantly higher than those of nonsmokers. Statistically significant correlations among the five PAHs metabolites were shown. The results suggest that LC/MS/MS technology should be useful in the environmental health discipline.
Keywords
Biomarkers; Polycyclic aromatic hydrocarbons (PAHs); LC/MS/MS; Monohydroxylated PAHs; Smoke;
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