• Current Research on Agriculture and Life Sciences
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• v.11
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• pp.71-79
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• 1993

This study was conducted to obtain the basic information for the classification of Perilla spp. based on their major morphological characteristics for the 20 local collections of perilla distributed in Korea. The results of this experiment were summarized as follows : Several morphological characteristics were investigated. Seed color was classified to four groups with greshywhite, greshybrown, brown and darkbrown. The 1,000 grain weight, 11 accessions were below 3.5g, four accessions was 3.6g to 4.5g and five accessions was 4.5g to 5.07g. There was two types of stem color, red and green, 15 accessions was green and 5 accessions including Andongjaerae, Yeupsil, Ockdong, Cheongsongjaerae and Chugjaso were red color. In fatty acid composition was 60% in average. Among 20 accessions, Euseongjaerae showed the highest content(65%) and Yeongyangjaerae the lowest(54%) linolenic acid content. In the cytogenetical studies on the four accessions including Andong, Yeupsil, Yeongkwang and Daeyeong, the number of chromosomes were 2n=38.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.327-333
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• 2005

Dihydroxy-acid dehydratase (DHAD, 2,3-dihydroxy-acid hydrolyase, EC 4.2.1.9) is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acid isoleucine and valine. Although the enzyme have been purified and characterized in various mesophiles including bacteria and eukarya, the biochemical properties of DHAD has bee not yet reported from hyperthermophilic archaea. In this study, we cloned, expressed, and purified a DHAD homologue from the thermoacidophilic archaeon Sulfolobus solfataricus P2, which grows optimally at $80\;^{\circ}C$ and pH 3, in E. coli. Characterization of the recombinant S. solfataricus DHAD (rSso_DHAD) revealed that it is the dimeric protein with a subunit molecular weight of 64,000 Da in native structure. rDHAD showed the highest activity toward 2,3-dihydroxyisovaleric acid among 17 aldonic acid substrates Interestingly, this enzyme also displayed 50 % activities toward some pentonic acids and hexonic acids when compared with the activity of this enzyme to the natural substrate. Moreover, rSso_DHAD indicated relatively higher activity toward D-gluconate than any other hexonic acids tested in substrates. $K_m$ and $V_{max}$ values of rSso_DHAD were calculated as $0.54\;{\pm}\;0.04\;mM$ toward 2,3dihydroxyisovalerate and $2.42\;{\pm}\;0.19\;mM$ toward D-gluconate, and as $21.6\;{\pm}\;0.4\;U/mg$ toward 2,3-dihydroxyisovalerate and $13.8\;{\pm}\;0.4\;U/mg$ toward D-gluconate, respectively. In the study for biochemical properties, the enzyme shows maximal activity between $70^{\circ}C$ and $80^{\circ}C$, and the pH range of pH 7.5 to 8.5. The half life time at $80^{\circ}C$ was 30 min. A divalent metal ion, $Mn^{2+}$, was only powerful activators, whereas other metal ions made the enzyme activity reduced. $Hg^{2+}$, organic mercury, and EDTA also strongly inhibited enzyme activities. Particularly, the rSso_DHAD activity was very stable under aerobic condition although the counterparts reported from mesophiles had been deactivated by oxygen.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.236-242
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• 1999

To examine the seedling stand and growth as affected by early seeding dates of dry direct seeded rice in the Honam plain area of Korea, Dongjinbyeo was seeded at six seeding dates from early March to late April in rice fields of silty loam soil(Jeonbuk series) at the National Honam Agricultural Experiment Station (NHAES) for two years, 1996 and 1998. Seedling stand decreased slightly. with an early seeding date, but it produced more than the optimum seedling number except for the seeding of 25 March in 1996. Days to emergence was significantly longer, as seeding date was earlier, and days to emergence by early seeding was shortened only by 8 days because the mean air temperature was lower in 1996 than average, while in 1998, the reduction effect was nine to twenty five days because the mean air temperature was higher than average. In early seeding, various weeds occurred at the emergence date of rice and dominant weeds were Alopecurus aequailis, Ludwigia prostata and Rorippa islandica. NH$_4$$^{+}$ -N content in the soil at the 5th leaf stage and maximum tillering stage were lower, as the seeding date was earlier when nitrogen was split applied as basal and top dressed in 1996, while it was not significantly different among seeding dates when nitrogen was intensively applied as a top dressing in 1998. Tiller number at the maximum tillering stage and panicle number/m$^2$ were more, as seeding date was earlier in 1996, while it was not different in 1998. Filled grain rate and 1,000 grain weight was not different among the seeding dates. Milled rice yield was significantly decreased in the seeding before the middle of March, but in the seeding after late March, it was not varied when compared with the normal seeding date in 1996, while in 1998, there was no difference among seeding dates. From the above results, in consideration of seedling stand, weed occurance, rice growth and milled rice yield, the critical optimum early seeding time in the southern plain area may lie in early April. But it was suggested that when soil moisture is proper for seeding practices, seeding amount is increased and nitrogen is applied after plumule emergence of rice, milled rice yield may not be reduced in the seeding of middle or late March, compared with the seeding in April.l.

• Journal of Animal Science and Technology
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• v.44 no.4
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• pp.399-406
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• 2002

An experiment was conducted to investigate the effects of supplemental copper or/and zinc methionine chelates(Cu-Met or/and Zn-Met) on the performance, nutrient digestibility, serum IgG level, gizzard erosion, and the contents of Cu and Zn in liver and excreta of broiler chickens. One thousand d-old broiler chickens (Ross$^{(R)}$) were assigned to 4 treatments: control, 100 ppm Cu in methionine chelate(Cu-Met), 100 ppm Zn in methionine chelate(Zn-Met) and 100 ppm Cu plus 100 ppm Zn in methionine chelate(Cu-Zn-Met). Each treatment had five replications of 50 (25 male + 25 female) birds each. Average weight gains of chicks fed chelated Cu or/and Zn were significantly higher than that of chicks fed the control (P<0.05). Moreover, feed conversion rates of chicks were better in the chicks fed chelated Cu or/and Zn than in the chicks fed the control (P<0.05). The birds fed the chelated Cu and Zn(Cu-Zn-Met) tended to perform the best growth rate and feed conversion rate. Nutrient digestibilities were not affected by the dietary treatments. Serum IgG level of chicks fed Cu-Zn-Met was significantly higher than that of chicks fed the control (P<0.05). Gizzard erosion index was not significantly different among the treatments. The contents of Cu and Zn in liver were not significantly affected by the dietary treatments. The excreta contents of Cu or/and Zn were significantly high in the birds fed supplementary Cu or/and Zn. It was concluded that dietary supplementation of Cu or/and Zn in methionine chelated form improved growth and feed conversion efficiency of broilers.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.150-154
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• 2007

This study was carried out to increase germination by classifying seeds as seed coat colors of three species of genus Pinus. As result, the seeds classified as colors showed a difference. In seed length, Pinus densiflora (Pd) and P. thunbergii (Pt) had no difference while P. rigitaeda (Pr) had a difference between light yellow seeds and dark brown seeds (p<0.05). Only Pt was different in seed width. And in 1,000 seeds weight the three species showed a great difference while were not significantly different in seed moisture contents. In percent germination, all of the three species had a difference, especially that of Pr was very wide. Pd and Pt were not different in mean germination time but Pr showed a difference between light yellow seeds and dark brown seeds.

• Journal of Environmental Health Sciences
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• v.43 no.3
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• pp.167-175
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• 2017

Objectives: Children may be more exposed to certain environmental contaminants because they react with greater sensitivity and have different food intakes and breathing rate per unit of body weight compared to adults. The aim of this study was to determine general exposure factors such as skin surface area, period and frequency of exposure, and others among Korean children aged ${\leq}18$ years old. This study was carried out as the first of its kind in Korea. Methods: We developed 23 recommended exposure factors for Korean children aged 0 to 18 years by reclassifying raw data from the National Health and Nutrition Examination Survey and the National Statistical Information Service, as well as through investigation by experiments in the absence of related data. Results: Reflecting the activity patterns of about 9,000 children, the daily inhalation rates for long-term exposure ranged from $9.49m^3/day$ for children from birth to <2 years to $14.98m^3/day$ for children aged 16 to <18 years. The research found that Korean children spent an average of 22.21 hours indoors, 0.67 hours outdoors, and 1.12 hours in-transit every day. Young children (${\leq}2$ years old) spent approximately 34 more minutes outdoors on weekends than they did on weekdays. Conclusion: Various physiological variables in the human body reflect characteristics of children that can directly influence risk exposure. Therefore, the identification of general exposure factors based on Korean children is required for appropriate risk assessment.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.302-302
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• 2017

This study was conducted to evaluated the effects of different sowing time(October 22, February 20, March 3, March 13, and March 23) and sowing quantities(150kg/ha, 200kg/ha, 250kg/ha, and 300kg/ha) on growth of naked oat(Avena sativa L.) cultivar(Choyang-Gwiri) at a cultivation area in Iksan, south Korea. Heading times were delayed with later sowing times. In autumn seeding(Oct. 22) the ear was headed at April 30, in spring seeding(Feb. 20, Mar. 3, Mar. 13, and Mar. 23) heading times were respectively May 14, May 14, May 15, and May 19. Heading time of spring seeding was delayed about 3 weeks than autumn seeding. Ripening times were similar trends to the heading times. In autumn seeding ears were ripened at June 7, in spring seeding each times were respectively Jun. 15, Jun. 13, Jun. 20, and Jun. 20. Ripening time of spring seeding was delayed about 2 weeks than autumn seeding. Culm length and ear length were shortened in spring seeding, but number of plants per $m^2$ were increased. Number of grains per a ear were 106 in autumn seeding, but grains per a ear in spring seeding were respectively 88, 83, 83, and 73. Weight of 1,000 grains in spring seeding was heavier than that in autumn seeding, the weights were tend to light as later seeding times. Yield of grains was declined as later seeding times, yield of in autumn seeding was 2,900kg/ha, whereas that in spring seeding was 2,180kg/ha. The highest yield of spring seeding time was in Mar. 13, before this seeding time soil surfaces were severely dried as few rain fall, so germination was poor in those seeding times. As several seeding quantities were seeding, earing and ripening times were not different. but increasing seeding quantity, culm length was lengthened and ear length was shortened, number of plants per $m^2$ were increased and grains per a ear were reduced. Yield of grains were increased more seeding quantities, yield was highest up to 250kg/ha seeding quantity.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.327-327
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• 2017

The effect of different seedling ages and transplanting times on the growth and yield of Indica ${\times}$ Japonica rice for noodle processing was evaluated to develop a high yielding cultivation technology for increasing the competition against the imported foreign rice. Four seedling ages (10-, 20-, 30- and 33-day old) of two Indica ${\times}$ Japonica rice cultivars (cvs. Saemimyeon and Palbangmi) and three transplanting dates (May 20, May 30 and June 9) were used in the study. Our results showed that the growth and rice yield of the two cultivars were significantly affected by the different seedling ages and transplanting times. Dry matter production at the panicle heading of the two rice cultivars were generally higher in the 30-day old seedling than the other seedling age treatments and then gradually decreased as the transplanting time was delayed from May 20 to June 9. Similar high panicle number per square meter were recorded at the 30-day old seedling between May 20-May 30 transplanting times. In contrast, other yield parameters that includes spikelet number per panicle, 1,000-brown rice weight, and ripened grain ratio (except for the June 9 transplanting time of Palbangmi) were not significantly affected. The milled rice yield of Saemimyeon was higher than that of the Palbangmi regardless of seedling ages and it was also the highest at the 30-day old seedling with four seedling ages. The milled rice yield of Saemimyeon was only slightly decreased as the transplanting time was delayed from the May 20 to June 9 but Palbangmi had a significantly lower milled rice yield at June 9 transplanting due to the low ripened grain ratio. The result indicates that the most suitable seedling age for the cultivars tested was at 30-day old seedling for noodle processing rice and recommended transplanting times were between May 20 and May 30 for the high rice yield in the Yeongnam plain area.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.331-331
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• 2017

This study was conducted to know the influence of air temperature and sunshine duration on yield, chemical component, pigment color difference and starch characteristics of two colored rice cultivars in the plain area of Yeongnam province in Korea. The $L^*$, $a^*$, and $b^*$ value of brown rice in Hongjinju and Josaengheugchal rice cultivars was significantly different at continuous cultivated years, 2015 and 2016. The $L^*$, $b^*$ value of two colored rice was significantly increased in 2016 compared to 2015. The $a^*$ value of Josengheugchal rice cultivar was also significantly higher at 2016 than at 2015. It can be noticed the $a^*$, $b^*$, $L^*$ values in Josaengheugchal varied more than those in Hongjinju. Air temperature during ripening period in 2016 was higher than 2015, especially minimum temperature was too high to proper maturation for rice quality. In Josaengheugchal rice cultivar, sunshine duration after heading was longer in 2016 than in 2015. On the contrary, Hongjinju rice cultivar was ripened under condition of insufficient sunshine duration in 2016. The short growing duration by high temperature and long shiny duration made the lack of pigment for Josaengheugchal brown rice. In Hongjinju rice cultivar, shorten sunshine duration and higher night temperature were the source of the pigment deficiency. The grain size of rice which produced in 2016 was bigger than that of 2015 in both rice cultivars. The 1,000 grain weight of rice from 2016 was also bigger than that of 2015. Head rice ratio was high in the rice cultivars produced in 2015. Protein of milled rice in 2016 was more decreased than that of 2015 in Josaengheugchal rice cultivar, it showed reverse result in Hongjinju rice cultivar. Amylose contents of milled rice in 2016 were more decreased than that of 2015 in Hongjinju rice cultivar. Branch chain length distribution of amylopectin was shown a distinct difference between Josaengheugchal and Hongjinju rice flours by each produced year. Josaengheugchal rice cultivar produced in 2015 had a higher amount of short chains than that of 2016 rice starches. In Josaengheugchal rice cultivar, the pasting temperature and peak, trough, breakdown, final viscosity increased in rice flour which produced at 2016, whereas the setback viscosity and peak time showed lower value than those of rice from 2015. The most pasting properties (except of setback viscosity) of rice starch in Hongjinju rice cultivar grown in 2015 were higher than those of rice cultivar produced in 2016.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.250-250
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• 2017

Studies were conducted to select the optimum sowing date and planting density of sesame in the middle area of Korea. To select the optimum sowing date, sesame seeds sowed from 20 April to 7 July with 15-day intervals. To select the optimum planting density, sesame seeds investigated under four different planting distances ($30{\times}10,\;30{\times}15,\;30{\times}20$, and $30{\times}25cm$)respectively in the experimental field. As seeding date was delayed, days to emergence were shortened flowering and maturing date were delayed. Delayed sowing date resulted in decreased length, capsule setting stem length and number of capsules, and branchs per plant. Number of Capsules was high sowing date on 5 May in the range of 90~95ea/plant in sesame. Also yield of sesame seeds was most high on 5 May in the range of 142kg/10a by sowing date. Sowing date up to 5 May showed no effect on grain yield, but from 5 June to 5 July decreased 27%, 68% and 86%, respectively. For all planting distances, weight of 1,000 grain was not significantly different. However, number of branches and capsules tended to increase. Number of Capsules was high planting distance of $30{\times}20cm$ and $30{\times}25cm$ in the range of 146.7~165.7ea/plant in the Geonbaekkae. Areumkkae also showed the same tendency on planting distance of $30{\times}20cm$ and $30{\times}25cm$ in the range of 122.0~147.5ea/plant, respectively. Yield of Geonbaekkae and Areumkkae seeds was most high 116kg/10a, 117kg/10a, respectively on planting distance $30{\times}20cm$. Decreased in the planting distance of sesame has increased the incidence of disease and lodging. Based on the results, we suggest a planting distance of $30{\times}20cm$ maximal growth and yield of sesame in the middle area of Korea. Considering growth characteristics, sesame yield ability, the optimum sowing date was 5 May and optimum planting pattern was founded to be two rows planting in one ridge and planting densities was $30{\times}20cm$.