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Determination of Soluble Carbohydrates in Soybean Seeds

  • Choung Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.5
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    • pp.319-324
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    • 2005
  • This study was conducted to identify the soluble carbohydrates in soybean seeds using on-line HPLC-RID-ES/MS and HPLC behavior, and to deter­mine their contents for high quality soybean breeding. The monosaccharide (glucose) and three oligosaccharides (sucrose, raffinose, and stachyose) were identified in Korean soybeans by their chromatographic behavior and results of on-line HPLC-RID-MS with Electro­spray Ionization mode. On the basis of HPLC with a RID detector, the 32 Korean major soybeans contain $0.37{\pm}0.26\%$ glucose, $4.55{\pm}0.91\%$ sucrose, $1.19{\pm}0.19\%$ raffinose, and $2.72{\pm}0.37\%$ stachyose on a dry basis. In 468 soybean germplasms, the ranges of glucose, sucrose, raffinose, and stachyose were $0.03 - 0.98\%$, $2.33 - 6.96\%$, $0.08 -1.87\%$ and $0.75 - 3.18\%$, respectively. Among 500 soybean samples, oligosaccharide contents of 32 Korean major cultivated soybeans and 468 soybean germplasms were varied $5.83 - 10.06\%$ and $3.66 - 10.32\%$, respectively. The composition of glucose, sucrose, raffinose, and stachyose in soluble carbo­hydrates of 500 soybean samples were $2.07 {\pm} 1.75\%$, $58.01{\pm}5.82\%$, $10.13{\pm}2.28\%$ and $29.80{\pm}4.54\%$, respectively. Sucrose appeared to be most prevalent in soy­bean soluble carbohydrates.

A Study on the Usefulness of Total Hip Replacement Arthroplasty Using Fluoroscopy (투시하에 시행한 고관절 확대계측 촬영술의 유용성에 관한 분석)

  • Lee, H.J.;Son, S.Y.;Um, J.Y.;Chin, J.H.;Kim, K.C.
    • Journal of radiological science and technology
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    • v.22 no.1
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    • pp.21-26
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    • 1999
  • To make a comparative analysis for clinical application of total hip replacement arthroplasty(THRA) using fluoroscopy, we have performed total hip replacement arthroplasty making 30 patients an object with general radiography and fluoroscopy respectively. The results are as follows : 1) Reexamination rate was 80% of patients and mean 3.9 sheets in the general radiography, where as it was 46.7% and 0.37 sheets in the fluoroscopy(p<0.01) 2) Add examination was 2 cases in the general radiography, but fluoroscopy was no add examination. 3) The total film sheet used was mean 10.16 in the general radiography and 6.73 in the fluoroscopy. 4) In the cause of reexmination and add examination, inaccurate position of patient accounted for 72.6% in the general radiography and poor exposure condition accounted for 45.5% in the fluoroscopy. Total hip replacement arthroplasty using the fluoroscopy decreased reexamination and add examination rate, for these reasons, this method was effective abatement of pain, exposured radiation dose, and examination time and so on.

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Prediction of Eggshell Ultrastructure via Some Non-destructive and Destructive Measurements in Fayoumi Breed

  • Radwan, Lamiaa M.;Galal, A.;Shemeis, A.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.7
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    • pp.993-998
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    • 2015
  • Possibilities of predicting eggshell ultrastructure from direct non-destructive and destructive measurements were examined using 120 Fayoumi eggs collected from the flock at 45 weeks of age. The non-destructive measurements included weight, length and width of the egg. The destructive measurements were breaking strength and shell thickness. The eggshell ultrastructure traits involved the total thickness of eggshell layer, thickness of palisade layer, cone layer and total score. Prediction of total thickness of eggshell layer based on non-destructive measurements individually or simultaneously was not possible ($R^2=0.01$ to 0.16). The destructive measurements were far more accurate than the non-destructive in predicting total thickness of eggshell layer. Prediction based on breaking strength alone was more accurate ($R^2=0.85$) than that based on shell thickness alone ($R^2=0.72$). Adding shell thickness to breaking strength (the best predictor) increased the accuracy of prediction by 5%. The results obtained indicated that both non-destructive and destructive measurements were not useful in predicting the cone layer ($R^2$ not exceeded 18%). The maximum accuracy of prediction of total score ($R^2=0.48$) was obtained from prediction based on breaking strength alone. Combining shell thicknesses and breaking strength into one equation was no help in improving the accuracy of prediction.

A trial of Drug use Evaluation of oral levofloxacin in the Hospitalized Patients (입원환자의 경구용 levofloxacin 약물 사용 평가)

  • Ro, Eun-Sook;Park, Yun-Hee;Bang, Eun-Mi;Kim, Won-Kyu;Lim, Keum-Sook
    • Korean Journal of Clinical Pharmacy
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    • v.19 no.2
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    • pp.105-109
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    • 2009
  • In July 2008, The National Police Hospital has developed the CPOE system(Computerized Physician Order Entry system) which links the medicine master and introduced the new program for the injectable antimicrobial agents. After introducing new system, we wanted to check the management of oral antimicrobial agents. Because new system has limitation that new system control only the use of the injectable antimicrobial agents. So we tried to evaluate the use of oral levofloxacin, which was chosen by a medical specialist of the infection. We retrospectively analyzed the appropriateness of oral levofloxacin through EMR(Electric Medical Record) of 72 inpatients who received oral levofloxacin in National Police Hospital in December 2008. We applied the modified ASHP(American Society of Health- System Pharmacists) DUE(Drug Use Evaluation) criteria, which is composed of justification of drug use, critical indicators, complications and outcome measures. Acceptable cases of use of levofloxacin were 67(93.01%) and 57cases(79.2%) showed good outcome. However critical indicators were not performed very well. We want to suggest the new system for the management of the medical therapeutics by the pharmacist in charge.

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Prediction of Mechanical Behaviors of Bio-mechanical Materials (생체공학용 척추경 나사의 기계적 거동 예측)

  • Park, Joon-Sik;Choi, Jin Hwa;Cho, Myeong-Woo;Choi, Gil-Woon
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.3 no.1
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    • pp.72-78
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    • 2004
  • In this paper, mechanical behaviors of developed pedicle screw system, made of bio-mechanical materials(Ti-6Al-4V, Grade 5), ale predicted using FEM analysis. As a first step, morphologic construction of normal Korean spines and surgical operation convenience are considered to design optimum pedicle screw system. In this step, various design variables are considered as design parameters to develop optimized models. As a next step, tension and bending tests are performed to improve the structural performance of the developed system using finite element method. In this step, required Static compression and bending test specifications by ASTM F-04 25 04 01 are applied to understand the bio-mechanical behaviors of the designed spinal implant system under various load types. As the results of this research, it is possible to develop efficient pedicle screw system, having enough rigidity and fixation to stand any spinal damage under allowable stress conditions.

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Study on the Novel Materials Containing Nanoparticles and Isocyanate Group for Strength Improvement of Hydrogel Ophthalmic Lens

  • Lee, Min-Jae;Sung, A-Young
    • Journal of Integrative Natural Science
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    • v.11 no.2
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    • pp.113-120
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    • 2018
  • This study was planned to prepare the high strength hydrogel ophthalmic lens containing isocyanate group and nanoparticles. HDI with carbon nanoparticles were used as additives for the basic combination of HEMA, MA and MMA, and the materials were copolymerized with EGDMA as the cross-linking agent and AIBN as the initiator. The mixture was heated at $100^{\circ}C$ for an hour to produce the high performance hydrogel ophthalmic lens by cast mold method. Measurement of the physical characteristics of the produced material showed that the refractive index was in the range of 1.4027~1.4600, water content 25.21~44.01%, contact angle $54.18{\sim}72.94^{\circ}$, visible light transmittance 53.03~92.09%, and tensile strength 0.1024~0.2359 kgf and breaking strength was 0.0872~0.2825 kgf. The results showed an increase of refractive index while the decrease in water content. And also, the breaking strength was highest when the addition ratio of HDI was 5%(wt). As a result of the absorbance measurement, no significant difference was observed in all the samples, so it can be judged that the stabilization of nanoparticles in the polymer was maintained.

Analysis of Greenhouse Gas Emission associated with Clean Energy Agriculture System Development (청정에너지농업시스템 개발에 따른 실증단지의 온실가스배출량 분석)

  • Kim, Tae-Hoon;Yoon, Sung-Yee
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.643-658
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    • 2015
  • This study presents detailed emission of greenhouse gases of using Clean Energy Agriculture System according to a cradle-to-gate life-cycle assessment, including emission from energy use and leak of Biogas. Calculations were done with the PASS software and the covered gases are $CH_4$, $N_2O$ and $CO_2$, Total GHG fluxes of amount to $1719.03kgCO_2/day$, $39.63kgCO_2/day$ (2.31%) are from facility house process, $0.19kgCO_2/day$ (0.01%) are from transport process, $696.72kgCO_2/day$ (40.53%) are from Anaerobic digestion process, $846.61kgCO_2/day$ (49.25%) are from Heating and cooling system, $135.88kgCO_2/day$ (7.90%) are from Fertigation production process. The results suggest that for effective reduction of GHG emissions from Facility house using clean energy. Reduction targets should address both the production process as defined by IPCC sectors and the consumption process. An LCA assessment as presented here could be a basis for such efforts.

Postoperative Evaluation of Funnel Chest - The role of radiologic indices -

  • 윤태진
    • Journal of Chest Surgery
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    • v.25 no.12
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    • pp.1416-1421
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    • 1992
  • There are many objective indices of operative outcome of funnel chest, but there has been only a few report about the postoperative change of these indices and which is best correlated with subjective outcome. From January 1989 to December 1991, 74 patients with funnel chest underwent Ravitch operation and radiologic evaluation was performed on the twenty patients whose operative outcome were satisfactory by Humphreys` criteria and postoperative chest X-ray were available. Variable radiologic chest idices such as deformity grade [DG], lower vertebral index [LVI], upper vertebral index [UVI] and configuration index [CI] were measured preoperatively and 1, 6, 12 and 18 months pos-toperatively. The mean value of preoperative DG, LVI, UVI and CI were 5.32, 0.3, 0.26 and 1.01 respectively, and postoperative chages were as follows; DG; 3.13, 3.66, 3, 72, 4.0, LVI: 0.22, 0, 24, 0.25, 0.25 UVI; 0.27, 0.27, 0.27, 0.27 CI; 0.69, 0.77, 0.76, 0.79. The increments of CI were within the 90 percent confidence limit of normogram in most cases, but it was not true for LVI and UVI. And the differences between the observed and expected values of CI did not change significantly through the postoperative periods, which was not the case in LVI. We concluded from these results that 1] DG, LVI and CI were increased slowly during the postoperative follow up periods but the increments of CI were usually within the normal limits and so 2] CI can be used as a objective index of satisfactory outcome.

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Comparison of Ag-NORs stain and [3H]thymidine incorporation in antiproliferative effect of caffeine on NIH3T3 cells (Ag-NORs 염색법과 [3H]thymidine incorporation법에 의한 caffeine의 NIH3T3 세포증식 억제효과 비교측정)

  • Kim, Sung-ho;Lee, Cha-soo
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.459-464
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    • 1990
  • Inhibitory effect of caffeine on NIH3T3 cell proliferation was studied by using [$^3H$]thymidine incorporation and a modified one-step silver staining technique. The latter technique shows argyrophilic nucleolar organizer region-associated proteins (Ag-NORs), which are seen in nuclei as black dots. The result was compared with the counts of [$^3H$] thymidine incorporation. The results were summarized as follows; 1. The Ag-NORs numbers of NIH3T3 cells were $6.81{\pm}1.38$ at 24 hrs, $7.13{\pm}1.26$ at 48 hrs, $8.50{\pm}2.04$ at 72 hrs after incubation. 2. The numbers of Ag-NORs were significantly decrease in caffeine treated groups (p<0.01). 3. The counts of [$^3H$] thymidine incorporation were similar to the result of using Ag-NORs staining technique. It is concluded that Ag-NOR is a rapid, simple and compatible method to quantitate cell proliferation as compared with [$^3H$]thymidine incorporation.

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Physicochemical Factors Affecting Cooking and Eating Qualities of Rice and the Ultrastructural Changes of Rice during Cooking (쌀의 취반 및 식미특성에 영향을 주는 요인들과 취반 시 쌀의 배유 조직의 변화)

  • 이영은;오스만엘리자베쓰엠
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.6
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    • pp.637-645
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    • 1991
  • Physicochemical factors affecting cooking and eating quality of rice and their mechanisms were investigated. The stickiness of cooked rice was negatively correlated with amylose content(r=0.58, p<0.05) and protein content(r=-0.72, p<0.01), but not affected by crude fat content of rice. The ultrastructure of cooked rice grain showed the progressive gelatinization of starch from the periphery toward the center of the endosperm as water and heat energy diffused into. The rate of water diffusion appears to be dependent on the cell arrangement in the endosperm and the protein content of milled rice. Once water and heat reach the starch granules, the rate of in situ gelatinization of starches appears to be dependent on their own gelatinization temperature range and amylose content. Protein acts as a barrier for the swelling of starch and water diffusion in two ways : 1) by encasing starch granules in the starchy endosperm, and 2) by forming a barrier between the subaleurone layer and the starchy endosperm. Therefore, the separation and fragmentation of the outermost layers of the endosperm occurred more easily in the low-protein content rices, and was associated with increases of solids lost in cooking-water at 95$^{\circ}C$ and stickiness of cooked rice.

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