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Cadaveric Feasibility Study of Knotless Barbed Suture for Tracheal Anastomosis in Dogs

  • Hyeon-Ah Min;Chang-Hwan Moon;You-Jeong Jeong;Hae-Beom Lee;Dae-Hyun Kim;Seong Mok Jeong
    • Journal of Veterinary Clinics
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    • v.39 no.6
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    • pp.311-318
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    • 2022
  • Appropriate suture technique is crucial for successful tracheal anastomosis. However, standards for an ideal suture method have not yet been established. A previous study suggested tracheal anastomosis using barbed sutures that do not require knots; however, their use in small animals has not been reported. In this study, we aimed to compare knotless barbed sutures with conventional smooth sutures in terms of maximum tensile strength and suturing time in canine tracheal models to demonstrate the feasibility of using barbed sutures in tracheal anastomosis in dogs. Tracheal segments harvested from nine beagle dog cadavers were randomly assigned to three suture groups: barbed suture (B), smooth suture in simple interrupted pattern (SI), and smooth suture in simple continuous pattern (SC). The maximum tensile force and suturing time were compared according to the suturing method, and the mode of failure was evaluated. The average suturing time was 3.29 min in the B group; 4.41 min, SC group; and 8.99 min, SI group (p < 0.001). The average maximum tensile force in the SC group was 134.97 N, which was stronger than the SI (110.57 N) and B groups (103.10 N) (p < 0.05 and p < 0.01, respectively). The difference between the B and SI groups was not significant (p = 0.05). The B group demonstrated comparable mechanical strength and shorter suture time compared with the SI group. Therefore, tracheal anastomosis using barbed sutures could be an effective alternative to conventional smooth sutures in dogs.

Comparison of protein and amino acid content in selected cultivars of non-glutinous rice in Korea

  • So-Jung, Kim;Min Joo, Kim;Su Hyeon, Lee;Youngmin, Choi;Sung, Jeehye;Dongwon, Seo
    • Korean Journal of Agricultural Science
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    • v.48 no.4
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    • pp.1015-1026
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    • 2021
  • The objective of this study was to determine amino acid and protein contents of brown and milled non-glutinous rice of 13 cultivars in Korea. Protein contents of MRs (milled rices) were in order of Haepum (7.27%) > Hanareum No. 4 (7.14%) > Odae (6.84%). Protein contents of BRs (brown rices) were in order of Haepum (7.68%) > Odae (7.63%) > Hanareum No. 4 (7.60%). The amino acid content was the highest in Haepum (MR 5.76%, BR 6.49%), followed by Haedeul (MR 5.71%, BR 6.30%), and Odae (MR 5.63%, BR 6.29%). The essential amino acid contents of non-glutinous rices were in order of Haepum (MR 2.34%, BR 2.57%) > Haedeul (MR 2.31%, BR 2.48%) > Odae (MR 2.20%, BR 2.56%). The contents of amino acid and protein in BRs were considerably higher than those in MRs. Protein and most of amino acid contents were higher in Haepum than the other cultivars. The certificated reference material (CRM) 1849a (infant/adults nutritional formular) from National Institute of Standard and Technology (NIST) was used as the test sample to determine the precision and accuracy of the analytical method. The regression analyses revealed good correlations (correlation coefficient), greater than 0.99. The recovery values of the amino acids ranged from 93.17 to 99.59%. The limit of detection (LODs) ranged from 0.01 - 0.07 mg·100 g-1 and the limit of quantitation (LOQs) ranged from 0.03 - 0.21 mg·100 g-1 for all analytes.

The efficacy and efficiency of percutaneous lidocaine injection for minimizing the carotid reflex in carotid artery stenting: A single-center retrospective study

  • Hyung Kyu Lee;Tae Joon Park;Sang Pyung Lee;Jin Wook Baek;Seong Hwan Kim;Aiden Ryou
    • Journal of Cerebrovascular and Endovascular Neurosurgery
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    • v.26 no.2
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    • pp.130-140
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    • 2024
  • Objective: To assess whether local anesthetic infiltration could minimize the carotid baroreceptor reflex (CBR) which has an incidence after carotid artery stenting (CAS) that varies from 29% to 51%. Methods: This retrospective single-center study included 51 patients (mean age, 70.47 years) who underwent CAS for carotid stenosis. The groups included patients who underwent CAS for asymptomatic ischemic stroke (n=41) or symptomatic disease (n=10). Preprocedural percutaneous lidocaine injections (PPLIs) were administered to 70.6% and 5.9% of patients who underwent elective CAS and emergency CAS, respectively. Results: Among patients who received PPLIs, the mean degree of stenosis was 80.5% (95% confidence interval [CI]: ±10.74, 51-98%). The mean distance from the common carotid artery bifurcation to the most stenotic lesion (CSD) was 8.3 mm (95% CI: ±0.97, 6.3-10.2 mm); the mean angle between the internal carotid artery and common carotid artery (CCA) trunk (IAG) was 65.6° (95% CI: ±2.39, 61-70°). Among patients who did not receive PPLIs, the mean degree of stenosis was 84.0% (95% CI: ±8.96, 70-99%). The mean CSD was 5.9 mm (95% CI: ±1.83, 1.9-9.9 mm); the mean IAG was 60.4° (95% CI: ±4.41, 51-70°). The procedure time was longer in the PPLI group than in the no PPLI group (28.19 [n=39] vs. 18.88 [n=12] days) (P=0.057); the length of intensive care unit stay was shorter in the PPLI group (20.01 [n=36] vs. 28.10 [n=5] days) (P=0.132). Conclusions: Targeted PPLI administration to the carotid bulb decreased aberrant heart rates and blood pressure changes induced by carotid stent deployment and balloon inflation. As CBR sensitivity increases with decreasing distance to the stenotic lesion from the CCA bifurcation, PPLIs may help stabilize patients during procedures for stenotic lesions closer to the CCA.

Influence of Panax Ginseng on Hepatic DNA Synthesis in Mice Receiving ACTH ( II ) (고려인삼이 ACTH를 받은 마우스의 간 조직 DNA 합성능에 미치는 영향(II))

  • Chang, Won-Sang;Hong, Yong-Ha;Kim, Kee-Yun
    • The Korean Journal of Physiology
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    • v.8 no.2
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    • pp.75-78
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    • 1974
  • It was planned to investigate, by observing incorporation of $[^3H]$ thymidine into liver cells, the influence of Panax Ginseng upon hepatic DNA synthesis in mice that received ACTH. Thirty male mice $(body\;weight:\;18{\sim}20\;g)$ were divided equally into the ginseng-ACTH and the saline-ACTH groups. Each animal of the ginseng-ACTH and the saline-ACTH groups received every day (subcutaneously) 0.05 m1/10 g body weight of ginseng extract (4 mg of ginseng alcohol extract in 1 ml of saline) and the same amount of saline, respectively, for 5 days. On the 5th experimental day, all animals received 0.01 unit of ACTH intraperitoneally one hour. after the last medication, and $1{\mu}Ci/g$ body weight of $[^3H]$ thymidine after one more hour. Five animals, at a time, of each group were sacrificed 1, 10, and 24 hours after thymidine administration, and their hepatic radioactivity was measured autoradiographically in terms of the % number of radioactive cells in 1,000 cell counts (Radioactive Index, R.1.). Following results were obtained: 1. The hepatic radioactive indices obtained from the saline-ACTH group 1, 10, and 24 hr after $[^3H]$ thymidine administration were $1.50{\pm}0.32,\;2.16{\pm}0.33\;and\;2.79{\pm}0.31\;(mean{\pm}S.D.)$, respectively. 2. The corresponding values obtained from the ginseng-ACTH group $(2.71{\pm}0.22,\;3.85{\pm}0.29,\;and\;5.06{\pm}0.31)$ were significantly higher than the values of the saline-ACTH group. It is inferred from the above results that the ginseng tends to prevent reduction in hepatic DNA synthesis caused by ACTH administration.

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Ego Structure in Life Process of the Aged in Korea (노년기의 의식구조에 관한 연구)

  • 유숙자
    • Journal of Korean Academy of Nursing
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    • v.10 no.2
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    • pp.95-115
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    • 1980
  • Current statistics reveal remarkable prolongation of the average longevity in this country for the past decade. Welfare of the aged is no longer sole concern of the person or/and family. but has aroused social concern on the community and national level. This study was designed to assess social, economic and emotional needs of the aged. and to identify problems they are confronting. Data were gathered through questioning 273 subjects living in Seoul from July 25, to August 31. 19 80. Frequencies and percentile scores were analysed to describe the fact. and the significance of int or-variable differences was tested by Chi-square method. Results are : 1. Majority of the subjects (male : 65.38%). (female : 62.13%)“talk about past experiences”to re-collect their past days, the difference between male and female respondents was not significant. 2. Except few who earn their pocket money (4.21%). majority were doing household errands (34.52% ) and looking after their garnd children (29.26 %). Main sources of their pocket money revealed to be their children (84.02%) and their own savings (24.64% ). Except few (15%)engaged with social activities directly or indirectly. leisure hours are spent in chatting with aged neighbors (44.81%). Highest in the rank order on the joyous moments for the aged revealed to be when the members of family living apart paying a visit (male : 37.5%, female : 63.72%)difference of male and female was significant ( P<0.05). Among female respondents. significant difference between age group was revealed (p<0.05). 3. Majority prefered sin91e houses (84.30% ). as residential environment. the suburban (36.26% ) area was the filet in the rank order : difference between age group and the educational status were not significant. Majority of respondents revealed to have their own room in the house. The first preference was given to live with their children (68.86%). Memory of the past (37.36% )revealed to be the highest in the rank order among the reasons why they dislike moving the house. 4. Majority favored current welfare benefts provided for the old age. however. the ideal way to live at their old age they responded was to live on their own savings (50.54%). 5. Majority revealed to be daunted occasionally (62.27%) by not being less active (34.16%) socially and by poor physical health(29.75%). Male and female differ in the causes of loneliness significantly (P <0.001) : retirement (37.89%) in ale and helpessness (43.05%) in female revealed the highest in the rank order. Majority talk over their feelings with aged neighbors to overcome the loneliness. 6 Majority were in favor of planting and looking after pet animal in the house. however. male and female differ in the kind significantly (p <0.001), 7. Majority think about death and dying occasionally or more (84.11% ). Many of the respondents believes in the life after life (53.49%) : female revealed to be significantly higher (p <0.01). and subjects with christian belief were significantly higher than non-christians (P<0.001). Attitude towards death and dying differs significantly between male and female (P <0. 001) and between christians ans and nonchristians (p <0.001). Highest preference was given to simple funeral (69.85%). Precious heritage that they would pass on to their descendants was onoscience and ethical value(57. 51%) : christian response as the first value was christian belief (52.38%).

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Single Oral Dose Toxicity Study of Prebrewed Armeniacae Semen in Rats

  • Park, Ji-Ha;Seo, Bu-Il;Cho, Su-Yeon;Park, Kyu-Ryul;Choi, Seung-Hoon;Han, Chang-Kyun;Song, Chang-Hyun;Park, Soo-Jin;Ku, Sae-Kwang
    • Toxicological Research
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    • v.29 no.2
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    • pp.91-98
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    • 2013
  • Armeniacae semen (AS) has been considered a toxic herb in the Korean medicine as it contains hydrogen cyanide and amygdalin, especially in its endocarp. Therefore, prebrewed AS that is devoid of endocarp has been traditionally used. In the present study, amygdalin content of the prebrewed AS was significantly lower ($2.73{\pm}0.32{\mu}g/ml$; p<0.01) than the content in the extract that contained the endocarps ($28.50{\pm}6.71{\mu}g/ml$); amygdalin content corresponded to 10% of the extract in the present study. Because of single oral dose toxicity of prebrewed AS according to the recommendation of Korea Food and Drug Administration Guidelines (2009-116, 2009), which was based on single oral dose toxicity study of prebrewed AS, mortality due to toxic principles was significantly reduced. In this study, 2,000 mg/kg of prebrewed AS led to death of 1 female rat and 1 male rat at the end of 2 hr of administration. Based on these results, the 50% lethal dose in both male and female rats was determined to be 9279.5 mg/kg. Seizure, loss of locomotion, and increases in respiration and heart rate were observed as prebrewed AS treatment-related toxicological signs; these signs were restrictedly manifested in the prebrewed AS (2,000 mg/kg)-treated rats. In addition, no changes were observed in body weight, organ weight, gross features, and histopathological parameters with 2,000 mg/kg of AS in both male and female rats. These findings serve as direct evidence that amygdalin in AS is the toxic principle, which can be reduced by the traditional prebrewing method involving the exclusion of endocarp.

LIPID PEROXIDATION AND ANTIOXIDANTS IN WORMERS EXPOSED TO LEAD (연 폭로 근로자들의 지질과산화 및 항산화상태에 관한 연구)

  • Lee, Won-Jin;Hwang, Cheon-Hyun;Kim, Cheong-Sik;Chang, Soung-Hoon;Kim, Yang-Ho;Kim, Hae-Joon
    • Journal of Preventive Medicine and Public Health
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    • v.31 no.3 s.62
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    • pp.449-459
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    • 1998
  • This study was started to find out if plasma malondialdehyde(MDA), $\alpha-tocopherol$ and erythrocyte superoxide dismutase(SOD) activity could be markers of biological activity resulting from exposed to lead in workers. Blood samples were randomly obtained from lead-exposed workers(n=29), $CO_2$, welders(n=60) and office workers(n=60). We used whole blood to analyse blood lead with atomic absorption spectrophotometer. Superoxide dismutase activity in erythrocyte was measured with spetrophotometer. MDA and $\alpha-tocopherol$ in plasma were measured with high performance liquid chromatography. Lead-exposed workers was significantly high in blood lead concentration$(29.37{\mu}g/d\ell)$ compared with welders$(6.42{\mu}g/d\ell)$ and office workers$(5.01{\mu}g/d\ell)$. The level of plasma MDA was significantly higher in the lead-exposed workers($1.87{\mu}mol/g$ cholesterol) than the welders($1.41{\mu}mol/g$ cholesterol) and office workers($1.41{\mu}mol/g$ cholesterol). Erythrocyte SOD activity in lead-exposed workers(56.80 U/g Hb) was significantly increased than those of welders(37.63 U/g Hb) and office workers(20.47 U/g Hb). The plasma $\alpha-tocopherol$ level of lead-exposed workers($4.93{\mu}g/g$ cholesterol) was statistically different from welders($4.25{\mu}g/g$ cholesterol) and office workers$4.28{\mu}g/g$ cholesterol). Neither age nor smoking was related to SOD or MDA level. Blood lead was significantly correlated with erythrocyte SOD activity(r=0.405), plasma MDA(r=0.296) and $\alpha-tocopherol$ (r=0.207). Plasma MDA was also significantly correlated with SOD (r=0.217). In multiple regression analysis, the change of MDA and SOD activity level related to the blood lead concentration. These results suggested that the increase of plasma MDA and erythrocyte SOD activity in lead-exposed workers had a close relationship with the oxidative stress induced by lead.

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Influence of Service Quality and Visit the Motivation on Visitors Satisfaction, Behavioral Intention in Museum. -Focused on National Museum of Korea (박물관 서비스 품질과 방문동기가 관람객 만족도, 행동의도에 미치는 영향 연구 -국립중앙박물관을 중심으로)

  • So, Eun-Hye;Lee, Kyoung-Rul
    • The Journal of the Korea Contents Association
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    • v.14 no.1
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    • pp.515-527
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    • 2014
  • The purpose of the study is to find factors to measure Museum Service Quality (SERVQUAL) and Visitors' motivation. The survey was analyzed with 214 questionnaires out of 230 ones and it was conducted by those who have once visited the National Museum of Korea. Pre-test was conducted from May, 2013. Deriving adequate Service Quality Measures in Museum, factor analysis was applied. From 29th of July 2013 to 23rd of August 2013, surveys were conducted through online and off-line. Using the SPSS 20.0 statistics, Population analysis was applied frequently. In order to ensure the reliability, cronbach ${\alpha}$ value was evaluated. Fit of the research model and the suitability of the path coefficients were evaluated through AMOS to ensure the validity and reliability. As a result, in terms of Analysis on Service Quality, it influenced significantly on the degree of satisfaction. Visitors' motivation also had partial influence on the degree of satisfaction. Moreover, Behavioral Intentions were also influenced by the degree of satisfaction.

Phenolic contents and physiological properties of Pleurotus ostreatus by drying method and 30% fermented ethanol extraction for different periods (느타리버섯류의 자실체 건조 방법 및 주정 추출 기간별 페놀성 성분 함량 및 생리활성 효능 비교)

  • Yeob, So-Jin;Park, Hye-Sung;Kang, Suk-Min;Han, Jae-Gu;Lee, Kang-Hyo;Cho, Jae-Han
    • Journal of Mushroom
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    • v.14 no.4
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    • pp.215-219
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    • 2016
  • The total polyphenol and physiological activities of Pleurotus ostreatus 30% fermented ethanol using different drying methods and extraction periods were investigated. Based on the observed polyphenol content and physiological activity, freeze-drying showed better results than hot air-drying method for P. ostreatus extracted with 30% fermented ethanol for more than 15 days. The total phenolic compound content of 'Gosol' following thefreeze-drying method for 15 days showed the highest value of $0.49{\pm}0.02mg/mL$. Freeze-drying with extraction for 30 days for ASI 2344 showed the highest antioxidant activity based on the DPPH radical scavenging rate of $35.50{\pm}3.29%$. Freeze-drying 'Gosol' for 30 days resulted inthe highest anti-inflammatory and nitrite scavenging activity of $48.40{\pm}3.38%$. Our results showed that P. ostreatus is a functional food.

Effects of Seeding Dates on Lodging in Water Seeding of Rice (벼 담수표면 직파재배에서 파종기가 도복에 미치는 영향)

  • 송동석;김용재;이성춘
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.2
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    • pp.157-167
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    • 1996
  • The method of direct seeding on flooded paddy surface in rice is known to be the most labor saving cultural practice in rice. However, this method has a problem in practical use such as severe lodging occurring at the reproductive growth stage. The objectives of this study were observated degree of field lodging and variation of lodging-related characteristics with different seeding dates. The number of seedlings per m$^2$ were from 91 to 144 plants, and seedling ratios were from ranged from 61.7% to 91.8%. Days from seeding to flowering were shortened from 5 to 15 days by the later seeding dates. Heading dates of Ilpumbyeo, Seoanbyeo, Daecheongbyeo and Donjinbyeo on June 9 showed slightly elapsed on the critical stable heading time from, August 28 to 29. The culm length was effective in longer clum varieties than semidwarf varieties. The degree field lodging (degree of lodging: 0~9< 9 : complete lodging) in Obongbyeo and S101 with semidwarf varieties were 0.17, whereas 1.25 in Dongjinbyeo with long culm. The lodging resistant varieties and later seeding dates shortened the length of fibrous in the clum, and thickened fibrous in the clum, respectively. Obongbyeo and Sl0l showed stronger resistance to field lodging. The lodging resistant varieties, Obongbyeo and Sl0l, showed lower values of lodging index from 1.03 to 1.15 than those of lodging susceptible varieties, Daecheongbyeo and Palgongbyeo ranged from 1.42 to 1.70. Bending moment with leaf sheath were greater in lodging resistant varieties (Obongbyeo and Sl0l),1510.0~1930.4g.cm, than those in the lodging susceptible varieties (Daecheongbyeo and Palgongbyeo), 1127.2~1287.6g.cm.

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