• Journal of the Korean Society of Radiology
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• v.84 no.2
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• pp.512-517
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• 2023

Transanastomotic pancreatic duct stent placement during reconstruction following pancreaticoduodenectomy is widely performed to prevent postoperative pancreatic fistulas and duct stenosis. However, stent-related complications, such as stent occlusion and migration, may occur. Here, we report a rare case of a migrated pancreatic duct plastic stent. After pylorus-preserving pancreaticoduodenectomy, the stent migrated to the jejunum and served as a nidus of the stent-stone complex, which developed jejunal obstruction. The stent-stone complex was removed by explorative laparotomy.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1635-1643
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• 2009

The aim of this study was to provide new insight into the mechanism whereby the housekeeping enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) locates to cell walls of Lactobacillus plantarum 299v. After purification, cytosolic and cell wall GAPDH (cw-GAPDH) forms were characterized and shown to be identical homotetrameric active enzymes. GAPDH concentration on cell walls was growth-time dependent. Free GAPDH was not observed on the culture supernatant at any time during growth, and provoked cell lysis was not concomitant with any reassociation of GAPDH onto the cell surface. Hence, with the possibility of cw-GAPDH resulting from autolysis being unlikely, entrapment of intracellular GAPDH on the cell wall after a passive efflux through altered plasma membrane was investigated. Flow cytometry was used to assess L. plantarum 299v membrane permeabilization after labeling with propidium iodide (PI). By combining PI uptake and cw-GAPDH activity measurements, we demonstrate here that the increase in cw-GAPDH concentration from the early exponential phase to the late stationary phase is closely related to an increase in plasma membrane permeability during growth. Moreover, we observed that increases in both plasma membrane permeability and cw-GAPDH activity were delayed when glucose was added during L. plantarum 299v growth. Using a double labeling of L. plantarum 299v cells with anti-GAPDH antibodies and propidium iodide, we established unambiguously that cells with impaired membrane manifest five times more cw-GAPDH than unaltered cells. Our results show that plasma membrane permeability appears to be closely related to the efflux of GAPDH on the bacterial cell surface, offering new insight into the understanding of the cell wall location of this enzyme.

• Journal of Environmental Health Sciences
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• v.32 no.5 s.92
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• pp.522-531
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• 2006

This study has been conducted to evaluate the influence of ambient heavy metals to the air quality and to find their sources in Ulsan. Korea. The distribution characteristics of ambient heavy metals with wind direction were investigated by using pollution-rose diagram. Carcinogenic risk for five trace heavy metals (Pb. Cd, Cr, Mn, and Ni), which are classified to carcinogenic material by US EPA, are assessed according to the classification system of USEPA and WHO. According to pollution-rose calculation on pollutants sources, Pb and Cu in Deoksin, which is an adjacent to Onsan industrial complexes, were $0.1058\;and\;0.3242{\mu}g/m^3$. These concentration levels are the highest at all sampling sites. From this result we could confirm that it was affected by Onsan industrial complex that is located northeast of Deoksin.,And the maximum concentration of Cd, Cr, and Ni were $0.0306,\;0.0102,\;and\;0.0146\;{\mu}g/m^{3} in Yeocheon respectively. Because Yeocheon is in the Mipo industrial complexes, which have many combustors, incinerators, and manufacturing facilities compared to other regions. In carcinogenic risk assessment, Pb, Ni, and Mn concentration level at six sampling sites were lower than the risk level guideline values ($10^{-5}$) of WHO. However, $Cr^{6+}$ and Cd concentration in Yeocheon were 60% and 205% higher than the guideline values of WHO. Therefore it is very important that the emission from industrial complexes were carefully managed and controlled to improve air quality in residential area.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.3085-3091
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• 2013

Associations between ABCB1 and XPC genetic polymorphisms and risk of developing colorectal cancer (CRC) as well as clinical outcomes in CRCs with chemotherapy were investigated. A case-control study was performed on the ABCB1 C3435T, G2677T/A and XPC Lys939Gln polymorphisms in 428 CRC cases and 450 hospitalbased, age and sex frequency-matched controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays. We observed that the ABCB1 3435CT or CC+CT variants were significantly linked with increasing risk of developing CRC (adjusted OR (95% CI): 1.814 (1.237-2.660), P=0.0022; adjusted OR (95% CI): 1.605 (1.117-2.306), P=0.0102, respectively). Moreover, the distribution frequency of XPC AC genotype or AC+CC genotypes also showed a tendency towards increasing the suscepbility for CRC (P=0.0759 and P=0.0903, respectively). Kaplan-Meier curves showed that the ABCB1 C3435T variant was associated with a tendency toward longer progression-free survival (PFS) (n=343, Log-rank test: P=0.063), and the G2677T/A variant genotypes (GT+TT+GA+AA) with a tendency for longer OS in postoperative oxaliplatin-based patients (n=343, Log-rank test: P=0.082). However, no correlation of the XPC Lys939Gln polymorphism was found with PFS and OS in patients with postoperative oxaliplatin-based chemotherapy (n=343). Our study indicated that ABCB1 polymorphisms might be candidate pharmacogenomic factors for the prediction of CRC susceptibility, but not for prognosis with oxaliplatin chemosensitivity in CRC patients.

• Fisheries and Aquatic Sciences
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• v.21 no.8
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• pp.24.1-24.9
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• 2018

The Korean bottom trawl survey has been deployed on a regular basis for about the last decade as part of groundfish stock assessments. The regularity indicates that they sample groundfish once per grid cell whose sides are half of one latitude and that of one longitude, respectively, and whose inside is furthermore divided into nine nested grids. Unless they have a special reason (e.g., running into a rocky bottom), their sample location is at the center grid of the nine nested grids. Given data collected by the survey, we intended to show how to appropriately estimate not only the survey index of a fish stock but also its uncertainty. For the regularity reason, we applied the systematic sampling theory for the above purposes and compared its results with a reference, which was based on the simple random sampling. When using the survey data about 11 fish stocks, collected by the spring and fall surveys in 2014, the survey indices of those stocks estimated under the systematic sampling were overall more precise than those under the simple random sampling. In estimates of the survey indices in number, the standard errors of those estimates under the systematic sampling were reduced from those under the simple random sampling by 0.23~27.44%, while in estimates of the survey indices in weight, they decreased by 0.04~31.97%. In bias of the estimates, the systematic sampling was the same as the simple random sampling. Our paper is first in formally showing how to apply the systematic sampling theory to the actual data collected by the Korean bottom trawl surveys.

• Molecules and Cells
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• v.28 no.1
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• pp.37-42
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• 2009

Iron binding lactoferrin (Lf) is involved in the control of cell cycle progression. However, the molecular basis underlying the effects of Lf on cell cycle control, as well as its target genes, remains incompletely understood. In this study, we have demonstrated that a relatively low level of ironsaturated Lf, Lf($Fe^{3+}$), can stimulate S phase cell cycle entry, and requires Akt activation in MCF-7 cells. Lf($Fe^{3+}$) immediately induced Akt phosphorylation at Ser473, which subsequently induced the phosphorylation of two G1-checkpoint Cdk inhibitors, $p21^{Cip/WAF1}$ and $p27^{kip1}$. The Lf($Fe^{3+}$)-induced phosphorylation of Cdk inhibitors impaired their nuclear import behavior, thereby inducing cell cycle progression. However, the treatment of cells with a PI3K inhibitor, LY294002, almost completely blocked Lf($Fe^{3+}$)-stimulated cell cycle progression. LY294002 treatment abrogated Lf($Fe^{3+}$)-induced Akt activation, and prevented the cytoplasmic localization of $p27^{kip1}$. Higher levels of $p21^{Cip/WAF1}$ were also detected in the cytoplasmic sub-cellular compartment as a measure of cellular response to Lf($Fe^{3+}$). Consequently, the degree of phosphorylation of retinoblastoma protein was enhanced in response to Lf($Fe^{3+}$). Therefore, we conclude that Lf($Fe^{3+}$), as a potential antagonist of Cdk inhibitors, can facilitate the functions of E2F during progression to S phase via the Akt signaling pathway.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.39
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• pp.4.1-4.7
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• 2017

Background: This study is a quantitative evaluation of the influence of the mentolabial angle on perceived attractiveness and threshold values of desire for surgery. Methods: The mentolabial angle of an idealized silhouette male Caucasian profile image was altered incrementally between $84^{\circ}$ and $162^{\circ}$. Images were rated on a Likert scale by pretreatment orthognathic patients (n = 75), lay people (n = 75) and clinicians (n = 35). Results: A mentolabial angle of approximately $107^{\circ}$ to $118^{\circ}$ was deemed the most attractive, with a range of up to $140^{\circ}$ deemed acceptable. Angles above or below this range were perceived as unattractive, and anything outside the range of below $98^{\circ}$ or above $162^{\circ}$ was deemed very unattractive. A deep mentolabial angle ($84^{\circ}$) or an almost flat angle ($162^{\circ}$) was deemed the least attractive. In terms of threshold values of desire for surgery, for all groups, a threshold value of ${\geq}162^{\circ}$ and ${\leq}84^{\circ}$ indicated a preference for surgery, although clinicians were least likely to suggest surgery. The clinician group was the most consistent, and for many of the images, there was some variation in agreement between clinicians and lay people as to whether surgery is required. There was even more variability in the assessments for the patient group. Conclusions: It is recommended that in orthognathic and genioplasty planning, the range of normal variability of the mentolabial angle, in terms of observer acceptance, is taken into account as well as threshold values of desire for surgery. The importance of using patients as observers in attractiveness research is stressed.

• Mycobiology
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• v.38 no.2
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• pp.102-107
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• 2010

We identified a gene for $\beta$-1,3-glucan synthesis (GBG1), a nonessential gene whose disruption alters cell wall synthesis enzyme activities and cell wall composition. This gene was cloned by functional complementation of defects in $\beta$-1,3-glucan synthase activity of the the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. Disruption of the GBG1 gene did not affect cell viability or growth rate, but did cause alterations in cell wall synthesis enzyme activities: reduction of $\beta$-1,3-glucan synthase and chitin synthase III activities as well as increased chitin synthase I and II activities. GBG1 disruption also showed altered cell wall composition as well as susceptibility toward cell wall inhibitors such as Zymolyase, Calcofluor white, and Nikkomycin Z. These results indicate that GBG1 plays a role in cell wall biogenesis in S. cerevisiae.

• Journal of Korean Neurosurgical Society
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• v.60 no.1
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• pp.15-20
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• 2017

Objective : Diagnosing tumor progression and pseudoprogression remains challenging for many clinicians. Accurate recognition of these findings remains paramount given necessity of prompt treatment. However, no consensus has been reached on the optimal technique to discriminate tumor progression. We sought to investigate the role of magnetic resonance perfusion (MRP) to evaluate tumor progression in glioma patients. Methods : An institutional retrospective review of glioma patients undergoing MRP with concurrent clinical follow up visit was performed. MRP was evaluated in its ability to predict tumor progression, defined clinically or radiographically, at concurrent clinical visit and at follow up visit. The data was then analyzed based on glioma grade and subtype. Resusts : A total of 337 scans and associated clinical visits were reviewed from 64 patients. Sensitivity, specificity, positive and negative predictive value were reported for each tumor subtype and grade. The sensitivity and specificity for high-grade glioma were 60.8% and 87.8% respectively, compared to low-grade glioma which were 85.7% and 89.0% respectively. The value of MRP to assess future tumor progression within 90 days was 46.9% (sensitivity) and 85.0% (specificity). Conclusion : Based on our retrospective review, we concluded that adjunct imaging modalities such as MRP are necessary to help diagnose clinical disease progression. However, there is no clear role for stand-alone surveillance MRP imaging in glioma patients especially to predict future tumor progression. It is best used as an adjunctive measure in patients in whom progression is suspected either clinically or radiographically.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.12
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• pp.1863-1870
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• 2018

Objective: The Bayesian first-order antedependence models, which specified single nucleotide polymorphisms (SNP) effects as being spatially correlated in the conventional BayesA/B, had more accurate genomic prediction than their corresponding classical counterparts. Given advantages of $BayesC{\pi}$ over BayesA/B, we have developed hyper-$BayesC{\pi}$, ante-$BayesC{\pi}$, and ante-hyper-$BayesC{\pi}$ to evaluate influences of the antedependence model and hyperparameters for $v_g$ and $s_g^2$ on $BayesC{\pi}$.Methods: Three public data (two simulated data and one mouse data) were used to validate our proposed methods. Genomic prediction performance of proposed methods was compared to traditional $BayesC{\pi}$, ante-BayesA and ante-BayesB. Results: Through both simulation and real data analyses, we found that hyper-$BayesC{\pi}$, ante-$BayesC{\pi}$ and ante-hyper-$BayesC{\pi}$ were comparable with $BayesC{\pi}$, ante-BayesB, and ante-BayesA regarding the prediction accuracy and bias, except the situation in which ante-BayesB performed significantly worse when using a few SNPs and ${\pi}=0.95$. Conclusion: Hyper-$BayesC{\pi}$ is recommended because it avoids pre-estimated total genetic variance of a trait compared with $BayesC{\pi}$ and shortens computing time compared with ante-BayesB. Although the antedependence model in $BayesC{\pi}$ did not show the advantages in our study, larger real data with high density chip may be used to validate it again in the future.