• Title/Summary/Keyword: 희석농도

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Effect of a Bioactive Substance Extracted from Rheum undulatum on Control of Cucumber Powdery Mildew (대황에서 추출한 생리 활성 물질의 흰가루병 방제 효과)

  • 백수봉;경석헌;김종진;오연선
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.85-90
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    • 1996
  • 대황 추출물(RK)과 화학약품 1, 8-dihydroxy anthraquinone(AK)을 제제화하여 오이 흰가루병에 대한 약효, 약해 및 어독성을 조사하였다. 제제화한 RK와 AK 약제를 하우스에서 500배, 1000배 희석농도로 처리했을 경우 오이 흰가루병에 대하여 100%의 방제효과를 나타냈고, pot에서 2,000배, 3,000배, 5,000배 희석농도로 처리했을 때 모두 75% 이상의 방제효과를 나타냈다. 또 노지에서 4,000배, 5,000배 희석농도로 처리해도 75.3% 이상의 방제효과를 나타냈다. RK 약제는 250배 희석농도에서 오이에 약해가 없었으나 AK 약제는 250배 희석농도에서 약해가 있었다. 어독성은 두 약제 모두 반수치사농도(TLm)가 2ppm 이상으로 독성은 낮은 것으로 판정되었다.

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Screening and Utilization of Antifungal Plant against Ginseng Anthracnose (Colletotrichum gloeosporioides) (인삼 탄저병에 대한 항균성식물의 탐색과 이용)

  • 도은수;길기정
    • Korean Journal of Plant Resources
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    • v.14 no.3
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    • pp.206-212
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    • 2001
  • Crude extracts from 20 species of plants 16 families were evaluated for their antifungal activities against Colletotrichum gloeosporioides, and three plant extracts of them were applied to Ginseng in order to investigate the usefulness for Ginseng anthracnose control. Among the crude extracts from 20 species being tested, it of A. cepa, R. undulatum and C. japonica showed considerably antifungal activity against mycelial growth and conidial germination of C. gloeosporioides.. Ginseng anthranose was controlled at 2% concentration of A. cepa and R. undulatum and 1% C. japonica extract, but control value of three plant extracts was inferior to Mancozeb WP. Phytotoxic symptoms were observed in the leaves of ginseng with exogenous foliage application of 10% concentration of three plant extracts and 2% concentration C. japonica extract but not observed at 1% concentration.

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Changes of Sperm Motility in Black Seabream (Acanthopagrus schlegeli) by Osmolality of Diluents (희석액의 삼투질농도에 따른 감성돔 (Acanthopagrus schlegeli) 정자의 운동성 변화)

  • 장영진;임한규;장윤정
    • Journal of Aquaculture
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    • v.13 no.3
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    • pp.187-191
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    • 2000
  • The effects of osmolality on the sperm motility in black seabream (Acanthopagrus schlegeli) were studied. Sperm motility of black seabream was suppressed when the osmolality was equal to the seminal fluid. But sperm became motile when the osmolality increased in electrolyte solution (NaCl, KCl, $CaCl_2$, $MgCl_2$) and non-electrolyte solution (mannitol, glucose, fructose, sucrose). The changes of sperm motility index (SMI) by osmolality of diluents described a parabola. In all of the diluents, SMI was the highest at ca. 1,000 mOsm/kg, which is similar to the osmolality of seawater. Sperm motility was induced by osmolality of diluents, but exposure to hypotonic or hypertonic diluents was harmful to the sperm.

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Study on the Preservation of Liquid Boar Semen with $\textrm{BF}_5$ and Butschwiler Diluents (희석액 $\textrm{BF}_5$ 엔오투와 Butschwiler를 이용한 돼지 액상정액 보존에 관한 연구)

  • 천용민;박창식;서길웅;이규승
    • Journal of Embryo Transfer
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    • v.11 no.2
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    • pp.159-166
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    • 1996
  • 본 연구는 돼지 액상정액을 인공수정용 100ml 플라스틱 병에 보존하면서 BF5희석액과 Butschwiler 희석액 간에 보존 온도별 차이를 조사하고, BF5 희석액에서의 글리세롤 농도의 효과를 조사하여 돼지 액상정액을 좀더 장기간 사용할 수 있는 방법을 찾고자 실시하였다. 돼지 액상정액을 5$^{\circ}C$ 냉장고에 보존하면서 조사한 바에 의하면, 37$^{\circ}C$에서 0.5 및 2시간 배양후의 정자운동성은 전체 보존기간동안 BF5 희석액이 Butschwiler 희석액보다 유의하게 (P<0.05) 높게 나타났고, 정상첨체비율은 두 희석액간에 차이가 없었다. 돼지 액상정액을 15$^{\circ}C$에 보존하면서 조사한 바에 의하면, 3일부터 7일 보존시 까지 정자운동성과 정상첨체비율에 있어서 Butschwiler 희석액이 BF5 희석액보다 유의하데 높게 나타났다. BF5 희석액을 이용한 돼지 액상정액의 글리세롤 농도의 효과에 있어서는 최종 글리세롤 농도가 0, 2, 3, 및 5% 보다 1%일 때 가장 높은 정자운동성과 정상첨체비율을 나타내었다. 분만율, 복당 생존자돈수 그리고 출생시 평균 생시체중은 BF5 희석애과 Butschwiler 희석액간에 차이가 없었다. 이상의 연구 결과를 종합해 볼 때 BF5 희석액을 5$^{\circ}C$에서 Butschwiler 희석액은 15$^{\circ}C$에서 6-7일 동안 돼지 액상정액을 보존할 수 있었다.

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Ethanol Fermentation Characteristics in Chemostat Culture of Kluyveromyces fragilis (효모 Kluyveromyces fragilis의 연속 에탄올 발효 특성에 관한 연구)

  • 허병기;김진한류장수목영일
    • KSBB Journal
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    • v.4 no.3
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    • pp.253-258
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    • 1989
  • It was investigated that the influent sugar concentration and the dilution rate have an influence on the ethanol fermentation characteristics at steady state in a chemostat culture of K. fragilis using Jerusalem Artichoke juice as substrate. And also the optimum condition of high ethanol productivity and low residual sugar output in the ethanol production by K. fragilis, was clarified to be given at a dilution rate of 0.22/hr and at an influent sugar concentration of 85g/l.

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Effects of Oak Wood Vinegars on Mycelial Growth, Fruiting Body Production, and Mushroom Quality of Lentinula edodes (참나무류 목초액이 표고의 균사생장, 버섯 생산량 및 품질에 미치는 영향)

  • Shin, Keum Chul;Kim, Nam Kyu;Cho, Jong Won;Lee, Sang Yong;Lee, Jong Kyu
    • Journal of Korean Society of Forest Science
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    • v.97 no.1
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    • pp.35-44
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    • 2008
  • Mycelial growth of Lentinula edodes on solid or liquid culture media supplemented with differentconcentrations of oak wood vinegar varied depending on the types of wood vinegar or mushroom varieties used.Oak wood vinegar obtained from traditionally carbonizing kiln (TWV) inhibited mycelial growth of L. edodes atthe dilution level of less than $5{\times}10^{-2}$, but stimulated at $10^{-3}$ to $2{\times}10^{-3}$. Wood vinegar from mechanicallycarbonizing kiln (MWV) inhibited at $10^{-3}$, but stimulated at $2{\times}10^{-3}$. In liquid culture media, both wood vinegarinhibited at $5{\times}10^{-2}$, but stimulated at $2{\times}10^{-3}$. Sanjo-302-ho grown in liquid culture media at $2{\times}10^{-3}$, and Sanrim 2and 3-ho grown at $4{\times}10^{-3}$ showed relatively high degree of wood decay (DWD) and growing ability within wood(GAWW) when these isolates were inoculated onto oak wood logs. TWV completely inhibited mycelial growth ofgreen mold fungi, Trichoderma species, tested at $5{\times}10^{-1}$ dilution level, while MWV inhibited at $5{\times}10^{-1}$ to $5{\times}10^{-2}$dilution level. For Diatrype stigma, TWV inhibited mycelial growth at the dilution level of less than $5{\times}10^{-2}$, whileMWV did 80% of mycelial growth at $10^{-2}$, and 100% at $5{\times}10^{-1}$ dilution level. Fresh and dry weight of fruitingbodies harvested after soaking of wood logs into wood vinegar solutions with different concentrations werecompared, and were the highest at $2.5{\times}10^{-2}$ dilution level. Storage test of fruiting bodies at $10^{\circ}C$ for 10 daysshowed that fruiting bodies harvested after soaking in the solution with $2.5{\times}10^{-2}$ dilution level showed the bestfreshness by general test and color changes. In addition, shear force value of L. edodes fruiting bodies measuredby using texture analyzer showed that $2.5{\times}10^{-2}$ dilution level was the best concentration for keeping flesh texture.

Effect of Cooking Tools and Concentration of Egg Solution on Textural Characteristics in Coagulation of Egg Solution (조리기구와 난액의 농도가 알찜의 품질특성에 미치는 영향)

  • 이명숙;김기숙
    • Korean journal of food and cookery science
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    • v.7 no.1
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    • pp.53-59
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    • 1991
  • The purpose of this study is to investigate textural characteristics of egg solution coagulated with steamer and microwave oven, and egg solution diluted various concentration of it with water or milk. Textural Characteristics of the samples were evaluated by the Sensory test and Rheometer. The results obstained were as follow; 1. Rheometer measurement indicated that the thicker is concentration of egg solution, the harder is samples 2. In sensory evaluation on the various concentration of egg solution, the panels on the overall quality showed the most favorite tendency that the sample diluted with 50% of water and the one with 70% of milk in use steamer; the sample diluted with 30% of water and the one with 70% of milk in use microwave oven.

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Effects of Glucose and Ammonium Concentrations in Continuous Culture for Poly-$\beta$-hydroxybutyrate Production (Poly-$\beta$-hydroxybutyrate 생산을 위한 연속배양에서 포도당 및 암모늄 농도의 영향)

  • 이용우;유영제
    • Microbiology and Biotechnology Letters
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    • v.20 no.5
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    • pp.597-606
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    • 1992
  • Effects of dilution rate, inlet glucose and ammonium chloride concentrations on ,he performance of continuous culture of Alcaligenes eutrQPhus for poly-p-hydroxybutyrate (PHB) production were investigated. When inlet substrate concentrations were maintained constant (inlet glucose concentration = 20 g/l, inlet ammonium chloride concentration = 2 g/l), growth rate of residual biomass and PHB production rate showed its maximum at $0.1h^{-1}$ and $0.06h^{-1}$, respectively, and washout at $0.13h^{-1}$. PHB content decreased from 50% to 25% by increasing dilution rate, while specific PHB production rate increased continuously. Cell mass and PHB concentration gave its maximum values at inlet ammonium chloride concentration of 2 g/l and thereafter decreased, which showed the existence of substrate inhibition by ammonium. When inlet glucose concentration was 30 g/l, cell mass reached its maximum value, while PHB concentration increased continuously. The parameters of kinetic model were evaluated by the graphical and parameter estimation methods. The computer simulation results for the effects of dilution rate, inlet glucose and ammonium chloride concentrations fitted the experimental data very well.

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Development of Integrated Boration and Dilution Model for Boron Concentration Behavior Analysis (붕산농도 거동분석을 위한 종합적 붕산주입 및 희석모델 개발)

  • Chi, Sung-Goo;Park, Han-Kwon;Kuh, Jung-Eui
    • Nuclear Engineering and Technology
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    • v.24 no.1
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    • pp.30-39
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    • 1992
  • In this study, an integrated boration and dilution (INBAD) model is proposed to predict the required makeup flowrate for RCS boron concentration change and to analyze the boron concentration behavior at each subsystem within the RCS including CVCS during boration and dilution operation. The INBAD model is constructed by integrating an existing neutronic code and a boration and dilution model. The boration and dilution model has been developed for our specific purpose using the one-cell model and multi-cell model. In addition, in order to assess the boron concentration behavior more realistically, two important features such as variable pressurizer heater output and optional makeup mode (either direct or indirect injection) are implemented in this model. In order to demonstrate the usefulness of this model, the boron concentration behavior analysis at each subsystem were performed for both direct and indirect injection mode using YGN 3 and 4 design data. Also, the effect of pressurizer heater output on the primary loop boron concentration was investigated. The results showed that the boron concentration changes can be predicted accurately at each subsystem during boration and dilution operation.

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The Study on the Dilution Time of Radioactive Tracer in Estradiol Measurement (Estradiol 검사 시 방사성 추적자의 희석시간에 대한 고찰)

  • Lee, Hae Yeon;Seo, Han Kyung;Jang, Yi Sun;Kim, Hee Jeoung
    • The Korean Journal of Nuclear Medicine Technology
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    • v.21 no.2
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    • pp.44-48
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    • 2017
  • Purpose Estradiol (E2) is a steroid hormone mainly produced in women and is a useful indicator for diagnosis of gynecological diseases, menstrual cycle, menopause, and precocious puberty. E2 measurement is performed by diluting the $^{125}I$ radioactive tracer and tracer buffer in the kit. However, It was not precisely specified when the period of tracer is available after activating. The purpose of this study was to determine the appropriate dilution time based on the measurement value with dilution time. Materials and Methods From December 2016 to February 2017, 60 E2 samples with concentrations ranging from 8 to 4577 pg/mL were divided into low, medium, and high concentrations. Dilution of the $^{125}I$ tracer was performed on a 230 RPM agitator for 30 minutes, 1 hour 30 minutes, and 2 hours 30 minutes, respectively. 24 hour dilution was gently shaken and refrigerated. To verify the difference and significance of the results according to the dilution time, a test of normality was performed using SPSS 18.0 and analyzed by Kruskal-Wallis test. The measured value according to the dilution time was compared with the interquartile range of the absolute error. Results The results of Kruskal-Wallis test were not significant (P>0.05). Measurement results are showed as interquartile range of absolute error. At low concentration, it is 0.052 between 1 hour 30 minutes and 2 hours 30 minutes, and 0.105 between 30 minutes and 1 hour 30 minutes. At medium concentration, 0.062 between 30 minutes and 1 hour 30 minutes, and 0.038 between 1 hour 30 minutes and 2 hours 30 minutes. At high concentration, it is 0.029 between 1 hour 30 minutes and 2 hours 30 minutes, and 0.06 between 2 hours 30 minutes and 24 hours. Conclusion There were no statistically significant differences. However, the change in the measured value is the smallest between 1 hour and 30 minutes to 2 hours and 30 minutes. Therefore, we recommend diluting time between 1 hour 30 minutes and 2 hours 30 minutes.

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