• Title/Summary/Keyword: 황색 포도상구균

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Antimicrobial resistance of cleaner fabrics using natural dyes (천연염료로 염색한 Cleaner제품의 항균내구성)

  • Ma, Jae-Hyuk;Choi, Jeong-Rak;Kim, Eun-Hee;Ryu, Man-Hyun;Koo, Kang
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2011.03a
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    • pp.59-59
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    • 2011
  • 현재 우리들 사회의 환경과 생활이 윤택해 짐에 따라 문화생활을 영위하게 되고, 건강에 대한 의식이 커져가고 있다. 이에 건강을 유지하고 증진시킬 수 있는 환경 친화적인 제품에 대한 관심 또한 높아지고 있다. 특히 기존의 합성염료보다 천연염료로 염색한 제품은 고부가가치를 창출하며 환경친화적이고 인체친화적이다. 이를 바탕으로 Cleaner제품에 천연염색을 하고 천연항균제를 처리하여 제품의 세탁 후 항균지속성에 관하여 검토하였다. 본 연구에서는 대나무섬유와 N/P극세사 혼방소재에 꼭두서니와 치자로 염색한 뒤, 욕비 1:30으로 고정하고, 5%천연항균제 용액을 제조하였다. 제조된 용액에 시료를 5분간 침지 후, Pick-up율 70%로 조정하였다. 그리고 $80^{\circ}C$에서 20분간 건조 후, $110^{\circ}C$에서 1분간 열처리 하였다. KS K IOS 105-C06의 방법으로 30회 세탁하였다. KS K 0693과 AATCC 100의 시험방법으로 Staphylococcus aureus ATCC 6538(황색포도상구균)과 Klebsiella pneumoniae ATTC 4352(폐렴구균)의 균주에 대한 항균성을 측정하였다. KS K 0693의 경우 Staphylococcus aureus ATCC 6538(황색포도상구균)와 Klebsiella pneumoniae ATTC 4352(폐렴구균)에서 모두 99.9%의 정균감소률을 나타내었고, 또한 AATCC 100에서도 Staphylococcus aureus ATCC 6538(황색포도상구균)과 Klebsiella pneumoniae ATTC 4352(폐렴구균)에서도 모두 99.9%의 정균감소률을 나타냈다.

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A study on the awareness of methicillin-resistant Staphylococcus aureus and infection control among dental hygiene students in Yeungnam (영남지역 치위생과 학생의 메티실린내성황색포도상구균과 감염관리의 인식에 관한 조사연구)

  • Lee, Seung-Hun;Kim, Dong-Yeol
    • Journal of Korean society of Dental Hygiene
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    • v.11 no.1
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    • pp.29-38
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    • 2011
  • Objectives : The purpose of this study was to survey the awareness of methicillin -resistant Staphylococcus aureus(MRSA) among dental hygiene students in Yeungnam South Korea. Also the knowledge of infection control and infection diseases was surveyed. Methods : The subjects in this study were 213 dental hygiene students in three different colleges in Yeungnam. This study was conducted by using the self-reported questionnaire. Results : In the first, most of the students(86.9%) did not know about MRSA and 85.7% of them got the information in the college. Secondly, the awareness of infectious disease according to infection control education didn't have statistically significant difference(p>0.05). In the third, the infection control attitude level that the gowns should be changed after the treatment of infectious patients was scored lower than the others questions. on the other hand, the level of 'The use of high vacuum suction', 'The change of wet mask', 'The history taking of infectious disease' was scored higher. Finally, the attitude level according to grade and clinical training had statistically significant difference(p<0.05). But the infection control education had not affected significantly(p>0.05). Conclusions : Despite the infection control education and clinical training, the awareness was found insufficient in infectious diseases and MRSA. Therefore, it should be strengthen that the dental hygiene students were more educated about infection control at college course before clinical training, and especially the danger of MRSA was more.

Characteristics of Staphylococcus aureus Isolated from Patients with Diarrhea. (설사 환자에서 분리된 Staphylococcus aureus의 특성)

  • Park Eun Hee;Min Sang Gi;Lee Ju Hyeoun;Park Yon Koung;Jeong Gu Young;Bin Jae Hun
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.647-651
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    • 2005
  • The major causative bacteria of food poisoning were Salmonella spp. $(35.6\%)$, Staphylococcus aureus $(11.3\%)$ and Vibrio parahaemolyticus $(3.2\%)$ in our country. In this study we attempted isolation of S. aureus from stools of patients with diarrhea. Sixty-four strains $(9.1\%)$ were isolated from 704 the stools of patients with diarrhea. The enterotoxin was detected from 29 isolates $(45.3\%)$: 24 isolates $(37.5\%)$, 3 isolates $(4.7\%)$ and 2 isolates $(3.1\%)$ were A, B and C type, respectively. In the antibiotic susceptibility, 63 isolates $(98.4\%)$ were resistant to penicillin, 60 isolates $(93.8\%)$ to ampicillin, 35 isolates $(54.7\%)$ to erythromycin, 32 isolates $(50.0\%)$ to gentamycin, 22 isolates $(34.4\%)$ to tetracycline and 20 isolates $(31.3\%)$ to oxacillin. All of S. aureus isolates were susceptible to chloramphenicol and vancomycin, 20 isolates $(31.3\%)$ were methicillin-resistance S. aureus (MRSA). MRSA isolation rate was higher in male $(35.7\%)$ than female $(26.3\%)$. With the exception of two isolates which were resistant only to penicillin, sixty-one isolates were multiple antibiotic resistance.

Stimulatory Effect of Staphylococcal Protein A on Inflammatory Response in Human HaCaT Keratinocytes (사람의 피부상피세포에서 황색포도상구균의 독소인자인 Staphylococcal Protein A의 염증반응 촉진효과)

  • Kwon, Hyun-Jin;Kim, Yeon-Jung;Jang, Sung-Hee;Bae, Bo-Kyoung;Youn, Hwa-Young;Lee, Hee-Woo
    • Korean Journal of Microbiology
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    • v.47 no.4
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    • pp.348-355
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    • 2011
  • Staphylococcus aureus is a major human pathogen that is associated with various types of local and systemic infection. Staphylococcal protin A (SPA), a highly expressed surface component of S. aureus, may have a role in virulence such as activating inflammation and interfering with immune clearance. We examined the effect of recombinant SPA on inflammatory response in human HaCaT keratinocytes. The recombinant SPA protein was prepared using the pET-28a Vector System in Escherichia coli. The expression of pro-inflammatory related adhesion molecules and cytokines in HaCaT cells incubated for 6, 12, and 24 h with SPA (2 ${\mu}g$/ml) was analyzed by comparative RT-PCR or ELISA. The expression of E-selectin, ICAM-1, MCP-1, IL-6 and IL-8 was significantly increased in HaCaT from 6 to 24 h after treatment with SPA. SPA showed the effect on the adhesion-promoting ability of U937 monocytes to HaCaT cells. Our data demonstrate that SPA stimulates inflammatory response of HaCaT cells, implicating an important factor for exacerbation of skin inflammation of immunologic disease.

Evaluation of Microbiological Hazards of Hygiene by the Customers' Hands in University Foodservice Operation (대학급식소 고객의 손 위생에 대한 미생물학적 위해 평가)

  • Park, Hae-Jung;Bae, Hyun-Joo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.7
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    • pp.940-944
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    • 2006
  • The propose of this study was to evaluate the microbiological hazards of the customers' hands in university foodservice operation. A total of 190 customers' hands were sampled to test about aerobie plate counts, coliforms, E. coli and Staphylococcus aureus. The average number of aerobic plate counts was 3.11 log CFU/hand Ifrom 0.70 to 6.47 log CFU/hand) and that of coliform counts was 1.06 log CFU/hand (from not detected to 5.04 log CFU/hand). The mean level of aerobic plate counts (t=9.87, p<0.001) and coliforms (t=7.91, p<0.05) was significantly different by gender. E. coli was not detected, but Staphylococcus aureus was detected in 39 (20.5%) of 190 samples. The average of hand-washing frequency was 6.46 time per day. By the hand-washing frequency, the mean level of aerobic plate counts was significantly different (F=527, p<0.001), but coliforms was not significantly different. This study shows that it is needs to be given attention to hygiene handlers but also for customers in foodservice operations.

Antibacterial, Antioxidant Activities of Acer tegmentosum Maxim Ethanol Extract Against Staphylococcus aureus (벌나무 추출물의 황생포도상구균에 대한 항균 효과와 항산화 활성)

  • Jin-Woo Hwang;Chan Hwi Park;Hae-Yeon An;Ye-Won Jang;Hyun Kang;Sung-Gyu Lee
    • Journal of Plant Biotechnology
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    • v.49 no.4
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    • pp.339-346
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    • 2022
  • This study investigates the antibacterial and antioxidant activities of the ethanol extract of Acer tegmentosum Maxim (EATM) against Staphylococcus aureus. The total polyphenol and flavonoid content, 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging effect, and reducing power of the ferric reducing antioxidant power (FRAP) method were measured to verify the antioxidant activity of the EATM. The antibacterial activity against S. aureus using the EATM was verified by the paper disc method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The total polyphenol and flavonoid contents were 266.66 ㎍ GAE/mg and 6.46 ㎍ QE/mg, respectively. The DPPH and ABTS radical scavenging activity showed a concentration-dependent scavenging activity. The RC50 values of the EATM were 21.49 and 12.81 ㎍/mL, respectively. A FRAP analysis was conducted for evaluating the reducing power of the EATM and an efficacy of 0.73 ± 0.19 mM FeSO4 E/mg was observed. The antibacterial activity of EATM against S. aureus, determined using the paper disc method, showed an inhibitory ring of 3 mm at 2 mg. The MIC was confirmed at a concentration of ≥ 16 mg/mL, while the MBC was confirmed at 32 mg/mL. As the EATM shows antioxidant and antibacterial activities against S. aureus, it can be used as an effective antidote against atopic dermatitis.

Differences in the Antibiotic Resistance Pattern of Staphylococcus aureus Isolated by Clinical Specimens in a University Hospital in South Korea (일개 대학병원의 임상 검체에서 분리한 포도알균 항생제 내성 경향 차이)

  • Hong, Seong-No;Kim, Joon;Sung, Hyun-Ho
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.2
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    • pp.85-92
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    • 2018
  • Information on the prevalence of S. aureus and the current antimicrobial resistance profile is necessary in selecting the appropriate treatment of S. aureus infections in any part of the world. This study examined the frequency and antibiotic resistance list of S. aureus isolates obtained from clinical specimens at one hospital in Korea. A total of 1,746 gram positive cocci collected were identified as S. aureus. S. aureus isolates were obtained from different samples including sputum (N=565; 32.4%), endotracheal aspirate (358; 20.5%), wounds (329; 18.8%), blood (137; 7.8%), urine (67; 3.8%), and pus (59; 3.4%). All 1,545 S. aureus (100%) strains screened from sputum (565; 36.6%), endotracheal aspirate (388; 25.1%), wounds (329; 21.3%), blood (137; 8.9%), urine (67; 4.3%), and pus (59; 3.8%) were sensitive to glycopeptide (vancomycin, teicoplanin), oxazolidinone (linezolid) and stretogramin (quinupristin/dalfopristin). The prevalence of resistant S. aureus was significantly (P<0.01) lower in urine, blood, pus, wounds, and sputum than in endotracheal aspirates. As a result, there was a significant difference in the antibiotic resistance of S. aureus according to the clinical specimens.

급식소 조리종사자의 손 위생교육 효과 평가

  • 조현옥;배현주;전희정
    • Proceedings of the Korean Society of Food and Cookery Science Conference
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    • 2003.05a
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    • pp.100-100
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    • 2003
  • 단체급식소 조리종사자의 손 위생실태를 알아보기 위해 일반세균ㆍ대장균군ㆍ황색포도상구균에 대한 미생물 검사를 실시하고, 조리종사자의 개인위생 개선을 위해 개인위생의 중요성ㆍ각종 식중독의 발생현황 및 예방대책ㆍ손 위생의 중요성 등에 대해 단계별 위생교육 프로그램을 실시한 후 최종 미생물학적 검증을 통해 위생교육 효과를 평가하였다. 위생교육 전 손 위생실태는 작업 전의 경우 일반세균수는 4.04∼1.49 log CFU/hand, 대장균군수는 6.52 log CFU/hand 이하였고, 황색포도 상구균의 경우 전체의 10% 정도가 검출되었다. (중략)

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