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Stimulatory Effect of Staphylococcal Protein A on Inflammatory Response in Human HaCaT Keratinocytes  

Kwon, Hyun-Jin (Department of Laboratory Medicine, Daegu Fatima Hospital)
Kim, Yeon-Jung (Seoul Medical Center Research Institute, Seoul Medical Center)
Jang, Sung-Hee (Seoul Medical Center Research Institute, Seoul Medical Center)
Bae, Bo-Kyoung (Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University)
Youn, Hwa-Young (Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University)
Lee, Hee-Woo (Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University)
Publication Information
Korean Journal of Microbiology / v.47, no.4, 2011 , pp. 348-355 More about this Journal
Abstract
Staphylococcus aureus is a major human pathogen that is associated with various types of local and systemic infection. Staphylococcal protin A (SPA), a highly expressed surface component of S. aureus, may have a role in virulence such as activating inflammation and interfering with immune clearance. We examined the effect of recombinant SPA on inflammatory response in human HaCaT keratinocytes. The recombinant SPA protein was prepared using the pET-28a Vector System in Escherichia coli. The expression of pro-inflammatory related adhesion molecules and cytokines in HaCaT cells incubated for 6, 12, and 24 h with SPA (2 ${\mu}g$/ml) was analyzed by comparative RT-PCR or ELISA. The expression of E-selectin, ICAM-1, MCP-1, IL-6 and IL-8 was significantly increased in HaCaT from 6 to 24 h after treatment with SPA. SPA showed the effect on the adhesion-promoting ability of U937 monocytes to HaCaT cells. Our data demonstrate that SPA stimulates inflammatory response of HaCaT cells, implicating an important factor for exacerbation of skin inflammation of immunologic disease.
Keywords
adhesion; HaCaT keratinocytes; inflammatory response; staphylococcal protein A; U937 monocytes;
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