• Title/Summary/Keyword: 활성 경로

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Synthesis and Characterization of Water-borne Pressure Sensitive Adhesives Polymerized using Styrenated Phenol Type Surfactants (스티렌페놀계 계면활성제 기반 친환경 수계 점착제 합성 및 특성 분석)

  • Song, Young Kyu;Lee, Sang-Ho;Park, Young Il;Kim, Jin Chul
    • Journal of Adhesion and Interface
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    • v.21 no.4
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    • pp.156-161
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    • 2020
  • Waterborne pressure sensitive adhesives (PSA) has been received much attentions from both academia and industries as an environmental friendly-technology because it can significantly reduce use of hazardous organic volatile solvents. However, in the process of the mass production of waterborne PSAs, hazardous phenol type amphiphilic compounds have essentially been used as surfactants for the emulsion polymerization. For the reason, tremendous research efforts have been made to develop environment-friendly organic surfactant which can replace the phenol type surfactants. In this study, we verify the potential of a new class of surfactants based on the styrenated phenol derivatives as an alternative to the phenol type surfactants.

건강과 자연농업-제228호

  • Jeong, Jin-Yeong
    • THE HEALTH and ORGANIC FARMING
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    • no.228
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    • pp.1-12
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    • 2006
  • 학교급식,"친환경유기농 식단"이 대안/어린애들의 성조숙증에 경악하며/우리의 산야초/규산질비료에 대하여/벼 멀칭재배의 이론과 기술/"아이에게 과자 주느니 담배를 권하라"/노화의 주범, 활성산소를 없애자/아이들건강과 먹을거리 패러다임의 전환/아태 유기농업현황/"유기식품촉진 제도정비 절실"/두가지 비료로 유기농작물의 고품질 다수확 생산을 체계적으로 보다 쉽게 하는 방법/친환경 농산물 대량 소비처 발굴/벼 육묘시 발생하는 병해들/장마철,작물의 탄저병 방제/봉화 소천 친환경농업인/"유기농.축산자재 검증.인증"심포지엄/국내외 친환경농축산물의 생산 및 인증 실태/해충의 습성을 이용한 퇴치/6월중 새식구 명단/제주농업,몽골 땅에 유기농생산기지 건설/'06년도 경북도지부 지회장단 연찬회 개최/소비자 유기농업 교육 및 현장체험 행사/유기 및 일반 농산물의 품질 비교에 환한 연구/한국유기농업학회,친환경 유통.소비확대 방안 심포지엄/친환경농업 교과서 수록해야/전남여성농업인'친환경 실천대회'/당도진단/농촌진흥청,'친환경농자재 공시제 추진'

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Quercetin Inhibits Inflammation Responses via MAPKs and NF-κB Signaling Pathways in LPS-stimulated RAW264.7 Cells (마우스 대식세포 RAW264.7 세포에서 MAPK와 NF-κB 경로를 통한 quercetin의 염증 반응 저해 활성)

  • Woo Young, Won;Jeong Tae, Kim;Keun Ho, Kim;Ji Young, Hwang;Chung-Wook, Chung;Jong Sik, Kim
    • Journal of Life Science
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    • v.32 no.11
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    • pp.899-907
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    • 2022
  • Quercetin is one of bio-flavonoids which are abundant in fruits and vegetables and has been reported to have various pharmacological potentials such as anti-oxidation, anti-inflammation, anti-cancer, and anti-virus effects. In the present study, the anti-inflammatory effects and its working molecular mecha- nism of quercetin were investigated in mouse macrophage RAW264.7 cells. Quercetin significantly inhibited nitric oxide (NO) production in a dose-dependent manner without affecting cell viability and decreased inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 cells. In addition, quercetin decreased phosphorylation of p38, JNK, and ERK, and inhibited phosphorylation of NF-κB p65 protein and its inhibitor IκBα indicating that quercetin has the anti-inflammatory effects via regulation of MAPKs and NF-κB signaling pathway. We also detected expression changes of four kinds of pro-inflammatory cytokine genes (CSF2, IL-1β, IL-6, and TNF-α) with quantitative real-time PCR. The results showed that quercetin decreased the expression of four pro-inflammatory genes in LPS-stimulated RAW264.7 cells. Overall, our results showed that quercetin effectively suppressed inflammation responses induced by LPS in RAW264.7 cells via regulating MAPK and NF-κB pathway and down-regulating the expression of pro-inflammatory cytokine genes.

Effects of the Fermentation Periods on the Qualities and Functionality of the Vegetable Fermentation Broths (식물자원 발효액의 품질과 기능성에 미치는 발효기간의 영향)

  • Kim, Na-Mi;Lee, Jong-Won;Do, Jae-Ho;Park, Chae-Kyu;Yang, Jae-Won
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.6
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    • pp.293-299
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    • 2005
  • To determine optimal fermentation period of vegetables mixed with black sugar without innoculation of microorganisms, changes in chemical components, quality characteristics of the fermented broth and physiological functionality during the fermentation period were investigated, pH and $^{\circ}BX$ of the fermented broths were decreased gradually during the fermentation period. Except for radish, L and a color values of fermented broths were increased but b values were decreased during fermentation period. Viscosity of fermented broths of vegetables were decreased after 3 months of fermentation. ${\alpha}-Amylase$ activity in fermentation broth of brocolli, eggplant, cabbage, chicory, aralia, radish were increased to 460, 430, 180, 420, 560, 260 after six months fermentation period. In radish and tomato fermentation broth, invertase activity were increased to 200 and 460 units and cellulase activity were increased to 280 and 140 after six months fermentation period. The content of total phenolic compounds and electron donating ability were the highest after 2 to 4 months fermentation period and decreased thereafter. No significant level of tyrosinase inhibitory activity and SOD-like activity were observed. In the sensory evaluation test of aralia fermentation broth of droop, sweet and sour flavor and bitter, astringent taste were decreased during the fermentation period and droop tastes were highest in 3 months. In radish fermentation broth, radish flavor and pungent taste were decreased and sweet taste was increased during fermentation period. Acceptability in overall was the greatest after three months. Based on the results stated above, optimal fermentation period was appropriated 3 or 4 months.

Anti-inflammatory effect of a mixture of Astragalus membranaceus and Lithospermum erythrorhizon extracts by inhibition of MAPK and NF-κB signaling pathways in RAW264.7 cells (RAW264.7 대식세포에서 MAPK 및 NF-κB 신호전달 경로 억제를 통한 황기 및 지치 복합물의 항염증 효과)

  • Choi, Doo Jin;Kim, Geum Soog;Choi, Bo-Ram;Lee, Young-Seob;Han, Kyung Sook;Lee, Dong-Sung;Lee, Dae Young
    • Journal of Applied Biological Chemistry
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    • v.63 no.4
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    • pp.421-428
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    • 2020
  • This study investigated a mixture of Astragalus membranaceus (AM) and Lithospermum erythrorhizon (LE) extracts (ALM16), exerts anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells, and its underlying mechanism. ALM16 was prepared by mixing AM and LE extracts in a ratio of 7:3 (w/w). Cytotoxicity of ALM16 in RAW264.7 cells was not shown up to 200 ㎍/mL of ALM16. The results of this study showed that ALM16 does-dependently inhibits the production of nitric oxide, prostaglandin E2 and pro-inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor-α) in LPS-induced RAW264.7 cells. ALM16 not only markedly reduced the protein expression levels of inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 cells, but also inhibited the nuclear translocation and DNA-binding activity of nuclear factor-kappa B (NF-κB). In addition, ALM16 specifically inhibited the phosphorylation of c-Jun N-terminal kinase and extracellular signal-regulated kinases in LPS-stimulated RAW264.7 cells. In conclusion, these results suggest that ALM16 may exert anti-inflammatory effect by modulating mitogen-activated protein kinase and NF-κB signaling pathways.

Adhesive Properties of Emulsion PSA Polymerized Using Tween Series Nonionic Surfactants (Tween계 비이온 계면활성제를 이용하여 중합한 에멀션점착제의 접착물성)

  • Lim, Tae Kyun;Lee, Myung Cheon
    • Korean Chemical Engineering Research
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    • v.52 no.3
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    • pp.289-293
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    • 2014
  • In making emulsion type pressure sensitive adhesive (PSA), environmentally friendly Tween series nonionic surfactants were used to find out the possibility of substituting the traditional nonyl-phenyl nonionic surfactant (NP-40) by comparing their adhesive properties. Results exhibited that the PSA used Tween series nonionic surfactants showed much better adhesive properties in peel strength and holding power than that used NP-40.

The Brewing of Kochuzang (Red Pepper Paste) from Different Starch Sources - Part I. Proximate Component and Enzyme Activity during Koji Prepartion - (전분질(澱粉質) 원료(原料)를 달리한 고추장의 양조(釀造) - 제1보(第1報). 제국과정(製麴過程)중의 일반성분(一般成分)과 효소력(酵素力) -)

  • Lee, Taik-Soo;Cho, Han-Ok;Kim, Chul-Soo;Kim, Jong-Goon
    • Applied Biological Chemistry
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    • v.23 no.3
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    • pp.157-165
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    • 1980
  • Kojis were prepared from the different starch raw materials such as glutinous rice, barley rice, wheat flour and sweet potato, and were tested in an effort to reduce production cost and to improve quality of Kochuzang(red pepper paste). During the starting period of Koji preparation, there were less significant changes in pH; however, pH decreased somehow in the case of sweet potato, whereas it increased for the other Kojis. In general, the highest acidity was obtained after 72 hours of Koji preparation. Total nitrogen, soluble nitrogen and amino-nitrogen content increased in order of wheat flour, barley rice, and glutinous rice; Kojis they were markedly produced between 72 and 96 hours of Koji preparation. The maximum amount of reducing sugar was observed between 48 and 72 hours of Koji preparation during this period the reducing sugar content varied widely depending on starch source. Sweet potato Koji produced the highest level of ethyl alcohol content after 72 hours of Koji preparation; for the other Koji the same trends were observed after 24 hours. The starch liquefying activities have reached the highest level after 96 hours and for glutinous rice and barley rice; however, they kept on increasing until 120 hours for wheat flour and sweet potato. Koji Starch liquefying and saccharogenic amylase activities tended to increase in order of sweet potato, glutinous rice, barley rice and wheat flour. Kojis Various protease activities were measu-red during the Koji preparation, and they increased in order of alkali, neutral and acidic protease.

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Functional analysis of various effective microorganisms used in the farmer's fields (농업현장에서 활용되는 농업용 미생물의 기능분석)

  • Choi, Eun-Jung;Anandham, R.;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Jee, Hyeong-Jin;Suh, Jang-Sun;Kim, Yong-Ki
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2009.12a
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    • pp.310-311
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    • 2009
  • 농업현장에서 활용되는 농업용미생물의 효능을 평가하고 현장실용화를 제고할 목적으로 본 시험을 수행하였다. 8개 지자체를 대상으로 친환경농업 특성화 지원사업 현황을 조사하고, 지자체별 미생물 활용수준을 평가하였으며, 각 지자체별로 활용되는 미생물을 수집하여 항균활성, siderophore 생성, IAA 생성, 질소고정, 인산가용화 능력 및 작물생육에 미치는 영향을 분석하였다. 지자체에서 활용하는 미생물은 주로 유산균, 고초균, Bacillus속 세균, 광합성균, 효모 및 EM 등으로 몇가지 종류의 미생물을 활요하는 것으로 조사되었고, 배양을 위한 배지는 대부분 균주를 개발한 대학 또는 산업체에서 공급받는 것으로 나타났으며, 배양기술 측면에서 볼 때 단순 배양 보급하는 지자체가 2개소, 산-학-연간 공급네트워크 및 대량배양체계를 구축하여 미생물을 활용하는 지자체가 7개소, 미생물 대량배양체계를 구축하고 병해충 관리연구를 수행하는 지자체가 1개소 그리고 미생물 선발, 특성검정 및 효과 평가 등 개발 및 응용을 추진하는 지자체가 1개소인 것으로 나타나 지자체에 따라 활용기술 수준은 큰 차이를 보였다. 지자체 공급 농업미생물의 항균활성을 조사한 결과, 고초균, 효모, 유산균, 광합성균, Bacillus속 세균 모두에서 균주에 따라 항균활성을 보였는데, 특히 Bacillus 속 세균이 높은 빈도로 황균활성을 보이는 것으로 나타났다. 철 킬레이트 능력 (Siderophore 형성능)에 있어서는 5개 광합성 균주 중 1균주가, 5종의 효모 중 1균주만이 활성을 보인 반면, Bacillus속 세균(고초균 포함)의 경우에는 11개 균주 모두 활성을 보였다. 작물 생육과 밀접한 관계가 있는 IAA(Indole acetic acid)의 생성능에 있어서는 광합성균이 5균주 중 4균주가 활성을 보여 비교적 생성능이 높은 것으로 나타났으며, Bacillus 속 세균의 경우에는 11균주 중 8개 균주가 활성을 보여 광합성균 다음으로 높은 반도를 나타냈다. 효모의 경우에는 5균주 중 1개 균주가 IAA를 생성하는 것으로 나타났다. 질소고정능에 있어서는 광합성균은 5균주 중 3균주, Bacillus 속세균 11균주 중 6균주가, 효모는 5균중 1균주가 활성을 보인 반면, 유산균 중에는 단 한 균주도 질소 고정능을 보이지 않았다. 연작재배에 따라 토양 내에 축적되는 인산의 가용화능을 분석한 결과, 수집한 대부분의 유산균은 높은 활성을 보인 반면 기타 미생물은 인산가용화능이 거의 없는 것으로 나타났다. 결론적으로 Bacillus 속 세균은 항균활성이 높고, 작물생육과 밀접한 IAA 생성 및 질소고정능력을 보유 하고 있는 것으로 나타나 친환경 작물재배시 병 발생을 줄이고 작물생장을 촉진할 수 있는 미생물로 평가되었다. 농업현장에서 기능성 및 효능을 고려하여 미생물을 잘 활용하면 병 발생 관리 및 생산성 향상에 도움이 되리라 판단된다.

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Studies on the Microflora and Enzyme Activity in Processing of Accelerated Low Salt-Fermented Anchovy by adding koji (코오지를 이용한 속성 저식염 멸치젓의 미생물상과 효소활성)

  • 백승화;임미선;김동한
    • The Korean Journal of Food And Nutrition
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    • v.9 no.4
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    • pp.392-397
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    • 1996
  • To produce low salt fermented anchovy by an accelerated method with Asp. oryzae and Bacillus sp. koji, enzyme activity and variation of microflora during the 60 day fermentation were examined. Bacterial counts changed a little during the fermentation with the highest on day 40 for proteolytic and anaerobic bacteria and on day 20 for aerobic bacteria. Proteolytic, lipolytic, aerobic, and anaerobic bacteria counts were higher in the Bacillus sp. koji added anchovy paste than in others. The protease and lipase activities reached the highest point on day 20 and 30, respectively, and decreased gradually afterwards. The protease activity was higher in Asp. oryzae koji than in bacillus sp. koji, but the lipase activity was to the contrary.

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유류오염토양 정화를 위한 biopile의 현장적용

  • Jo Jang-Hwan;Park Jeong-Gu;Seo Chang-Il;Jeon Gwon-Ho
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.155-158
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    • 2005
  • 본 연구에서는 현장규모의 biopile을 제작하여 유류로 오염된 토양을 정화하였다. 오염토양의 TPH농도는 평균 2,800mg/kg(최고 3,590mg/kg)이었으며, 오염토양의 양은 $746m^3$이었다. Biopile $(17{\times}20{\times}2.5m)$을 약 3개월간 운전하며 일정 시간 간격으로 토양가스($O_2,\;CO_2)$ 및 VOCs) 및 토양의 TPH농도를 분석하였다. 지속적인 공기의 주입/추출에 의해 biopile내부는 호기조건을 유지하였으며, 미생물의 활성도 증가에 따라 이산화탄소의 농도가 증가하는 경향을 나타내었다. 또한 토양가스 분석결과 휘발성 유기오염물질(VOCs)은 약 40일 경과 후 90% 제거되었으며, 90일 경과 후 토양의 TPH 제거율은 98%로 나타났다.

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